Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,410 (T)7→6 100% coding (1280/1332 nt) gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4040T.82.8% 465.4 / 117.5 163coding (1274/1332 nt)gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base T (20/8);  new base . (57/78);  total (77/86)
Fisher's exact test for biased strand distribution p-value = 6.42e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 182 total aligned reads displayed.

ATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTAT  >  NC_000913/2470306‑2470500
                                                                                                  |                                                                                                
  tttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttt                                                                                                <  1:3749755/99‑1 (MQ=255)
   ttATGTGTCATTTTTCGGGGAAGGATTTAGAGATTTGAGCAAGCATTTATTTGGAATGTATTTTTATTTCCTCCTTTGCTTATACATAACAGTCGttttt                                                                                              <  1:2647174/100‑1 (MQ=255)
     aTGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGtttttta                                                                                            <  2:1519252/100‑2 (MQ=255)
     aTGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGtttttta                                                                                            <  1:856357/100‑2 (MQ=255)
      tgtgTCTTTGTTCGTGGAAGGATATAGATGTTTAAGCTAGCATTTATTTTGAATCTTTTTTTTGTTCGATCTTTGCTTAGAAATAACAGTCGtttttta                                                                                            <  1:3925502/99‑2 (MQ=255)
      tgtgTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaa                                                                                           <  1:1919214/100‑3 (MQ=255)
      tgtgTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaa                                                                                           <  1:1878238/100‑3 (MQ=255)
        tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaatt                                                                                         >  2:3034526/1‑96 (MQ=255)
        tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaatt                                                                                         <  1:2852838/100‑5 (MQ=255)
         gtCATTTTTCAGGTAAGGATATATTGATTTTAGCCAGCATTTATTTGGAATGTATTTTTCGTTCAACTTTTGCTTATACATAACAGTCGttttttaat                                                                                          <  1:653435/98‑4 (MQ=255)
             tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATaa                                                                                   <  1:1133581/100‑1 (MQ=255)
              ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATaaa                                                                                  <  2:92469/100‑1 (MQ=255)
              ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATaaa                                                                                  >  2:4178349/1‑100 (MQ=255)
              ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATaaa                                                                                  <  2:2823608/100‑1 (MQ=255)
                  cGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTTTTTTTTTTTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAaataa                                                                              >  1:3611857/1‑100 (MQ=255)
                   ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAaataat                                                                             >  1:1568333/1‑100 (MQ=255)
                      gAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTc                                                                           <  2:2943994/99‑1 (MQ=255)
                       aaGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCaa                                                                         <  2:2702239/100‑1 (MQ=255)
                        aGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCaaa                                                                        <  1:831430/100‑1 (MQ=255)
                         ggATATAGAGATTTAAGCAAGCTTTTATTTAGAATGTATGTATCGTTCAACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCaa                                                                         >  1:1650218/1‑98 (MQ=255)
                         ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAg                                                                       <  1:4197415/100‑1 (MQ=255)
                         ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAg                                                                       <  2:609914/100‑1 (MQ=255)
                         ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAg                                                                       <  1:3643109/100‑1 (MQ=255)
                          gATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGa                                                                      <  1:2201157/100‑1 (MQ=255)
                          gATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGa                                                                      >  2:3979347/1‑100 (MQ=255)
                          gATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGa                                                                      <  2:3854833/100‑1 (MQ=255)
                            tataAAGATTTAATCAAGCATTTATTTGAAATGCATTTTTCATTAAACCTTTACTTATAAATAACATTCTTTTTTTATTTTATAAAATAATTCAAAGaaa                                                                    <  1:1588476/100‑1 (MQ=255)
                             ataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAAt                                                                   >  2:62383/1‑100 (MQ=255)
                              taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTTTTTTTATTTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATc                                                                  >  1:2934235/1‑100 (MQ=255)
                              taGAGATTTAAGCAAGCATTTATTTGGAATGTATCTTTCGTTCGACATTAGCTTATACATAACAGTCGTTTTTTAACTTATAAAATAATTCAAAGaaa                                                                    >  1:4174015/1‑98 (MQ=255)
                               agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCa                                                                 <  2:1818989/100‑1 (MQ=255)
                               agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCa                                                                 <  2:3547861/100‑1 (MQ=255)
                               agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCa                                                                 <  2:690302/100‑1 (MQ=255)
                                gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                >  2:238133/1‑100 (MQ=255)
                                  gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTTTTTTTTTTTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCATGa                                                              >  1:2070631/1‑100 (MQ=255)
                                  gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                              <  2:513264/100‑1 (MQ=255)
                                   aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACTTTTGATTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAc                                                             <  1:995424/100‑1 (MQ=255)
                                   aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAc                                                             <  1:2261297/100‑1 (MQ=255)
                                    tttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACa                                                            >  2:3284456/1‑100 (MQ=255)
                                       aagcaagcATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                         <  1:3346411/100‑1 (MQ=255)
                                         gcaagcaTTTATTTGGAATGTATTTTTTTTTTTTTTTTTTCTTATACATAACAGTCGTTTTTTAATTTTTATTATTATTCAAAGAAATCAAGACAAt                                                          >  1:3649234/1‑97 (MQ=255)
                                         gcaagcaTTTATTTGGAATGTATTTTTTTTTTGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAg                                                        >  1:1724464/1‑99 (MQ=255)
                                          caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGc                                                       >  1:3315295/1‑99 (MQ=255)
                                          caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                      >  2:2625836/1‑100 (MQ=255)
                                          caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                      >  1:3656969/1‑100 (MQ=255)
                                           aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTACTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                     <  1:174926/100‑1 (MQ=255)
                                            agcaTTTATTTGGAATGTATTTTTCGTTTGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATTAAGACAATAGCGa                                                     >  1:3430648/1‑99 (MQ=255)
                                             gcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATg                                                   >  1:1262554/1‑100 (MQ=255)
                                               atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTa                                                 >  1:2229655/1‑100 (MQ=255)
                                                tttatttGGAATGTATTTTTCGTTCGACCTTTGCCTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACACTAGCGATGTaa                                                <  1:2317820/100‑1 (MQ=255)
                                                   atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                             >  2:1174593/1‑100 (MQ=255)
                                                     ttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCAc                                           <  1:1543564/100‑1 (MQ=255)
                                                     ttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCAc                                           <  1:434206/100‑1 (MQ=255)
                                                       ggAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTa                                         >  1:2095612/1‑100 (MQ=255)
                                                       ggAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTa                                         <  2:1626419/100‑1 (MQ=255)
                                                       ggAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTa                                         >  2:2301356/1‑100 (MQ=255)
                                                       ggAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTa                                         >  1:3251913/1‑100 (MQ=255)
                                                         aaTGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAg                                       <  2:3426913/100‑1 (MQ=255)
                                                          aTGTATTTTTTTTTTTACTTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGTACTTAg                                       >  1:2441488/1‑99 (MQ=255)
                                                          aTGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGt                                      <  1:3064597/100‑1 (MQ=255)
                                                          aTGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGt                                      <  2:299958/100‑1 (MQ=255)
                                                          aTGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGt                                      <  1:4002162/100‑1 (MQ=255)
                                                             tATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTa                                   <  1:1158926/100‑1 (MQ=255)
                                                             tATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTa                                   <  2:3142156/100‑1 (MQ=255)
                                                                ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAAt                                <  2:2777405/100‑1 (MQ=255)
                                                                     gttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGC                           <  2:1806020‑M1/100‑1 (MQ=255)
                                                                     gttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGC                           <  2:1724471‑M1/100‑1 (MQ=255)
                                                                     gttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTAAAATTGCGC                           >  1:2024746‑M1/1‑100 (MQ=255)
                                                                      ttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGGAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCG                          <  2:2070638‑M1/100‑1 (MQ=255)
                                                                      ttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCG                          >  2:3125017‑M1/1‑100 (MQ=255)
                                                                      ttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCG                          >  2:3152527‑M1/1‑100 (MQ=255)
                                                                       tcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGC                         >  2:1232070‑M1/1‑100 (MQ=255)
                                                                        cgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCC                        <  2:1258008‑M1/100‑1 (MQ=255)
                                                                         gACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCA                       <  2:15788‑M1/100‑1 (MQ=255)
                                                                           ccTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAAt                     >  2:976700/1‑100 (MQ=255)
                                                                                 cTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAtggc               >  1:3118173‑M2/1‑96 (MQ=255)
                                                                                  ttATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAtggcg              >  2:2031355‑M2/1‑95 (MQ=255)
                                                                                  gatctcataacAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAtggcg              >  1:2009163‑M2/6‑95 (MQ=255)
                                                                                   tataCATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAtggcgc             >  2:1196582‑M2/1‑94 (MQ=255)
                                                                                    ataCATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAtggcgcg            >  1:4316121‑M2/1‑93 (MQ=255)
                                                                                    ataCATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAtggcgcg            >  2:3189682‑M2/1‑93 (MQ=255)
                                                                                    ataCATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAtgattggcgcg            <  2:193225‑M1/100‑12 (MQ=255)
                                                                                    ataCATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAtgattggcgcg            >  1:960419‑M1/1‑89 (MQ=255)
                                                                                    ataCATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAtgattggcgcg            <  2:4050202‑M1/100‑12 (MQ=255)
                                                                                     taCATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAtggcgcgc           >  2:2558517‑M2/1‑92 (MQ=255)
                                                                                     taCATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAtggcgcgc           >  2:4039420‑M2/1‑92 (MQ=255)
                                                                                     taCATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAtggcgcgc           <  2:4389161‑M2/100‑9 (MQ=255)
                                                                                     taCATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAtgattggcgcgc           <  2:13316‑M1/100‑13 (MQ=255)
                                                                                       cATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAtggcgagatc         <  2:2009170‑M2/100‑11 (MQ=255)
                                                                                       cATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAtgattggcgcgcaa         <  2:1483020‑M1/100‑15 (MQ=255)
                                                                                       cATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGcac         >  2:724822/1‑100 (MQ=255)
                                                                                       cATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGcac         >  2:567872/1‑100 (MQ=255)
                                                                                       cATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGcac         >  2:1835627/1‑100 (MQ=255)
                                                                                        aTAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCATGAttggcgcgcaat       >  1:779791‑M2/1‑88 (MQ=255)
                                                                                          aaCAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCATGAttggcgcgcaattt     >  1:765567‑M2/1‑86 (MQ=255)
                                                                                           aCAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGc     <  1:1101265/100‑1 (MQ=255)
                                                                                           aCAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGc     <  1:2886804/100‑1 (MQ=255)
                                                                                            cAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCt    <  2:1546650/100‑1 (MQ=255)
                                                                                             aGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCATGAttggcgcgcaatttaaa  >  1:727192‑M2/1‑83 (MQ=255)
                                                                                             aGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCta   <  2:2377910/100‑1 (MQ=255)
                                                                                                  |                                                                                                
ATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTAT  >  NC_000913/2470306‑2470500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: