Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,183,350 Δ1 bp 19.6% intergenic (+27/+463) zupT → / ← ribB zinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,183,3500A.19.6% 553.7 / 131.7 199intergenic (+27/+463)zupT/ribBzinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (85/73);  new base . (27/12);  total (112/87)
Fisher's exact test for biased strand distribution p-value = 1.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

Only 100 of 202 total aligned reads displayed.

CTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGC  >  NC_000913/3183251‑3183449
                                                                                                    |                                                                                                   
  aTGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATg                                                                                                   <  2:838462/100‑1 (MQ=255)
  aTGGCGTACTATATGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATg                                                                                                   >  2:2894791/1‑100 (MQ=255)
   tGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATgg                                                                                                  <  2:4144022/100‑1 (MQ=255)
    ggCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGa                                                                                                 >  1:3051951/1‑100 (MQ=255)
     gCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGAc                                                                                                <  2:701033/100‑1 (MQ=255)
      cGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACa                                                                                               <  2:1461919/100‑1 (MQ=255)
       gTACTATGTGGAATGTCAGTGATGGAATTCACTTTAGTACTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAAAAGTTATACAGCGCATGGACaa                                                                                              >  1:3479598/1‑100 (MQ=255)
           tATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTc                                                                                          <  2:1150642/100‑1 (MQ=39)
           tATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTc                                                                                          <  1:2916070/100‑1 (MQ=39)
           tATGTGGAATGTCAGTGATGGGATACAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTc                                                                                          <  1:271432/100‑1 (MQ=38)
           tATCTGGAATGTCAGTGATGAGATGCAGTTTAGCGCTGCTACAAACGGCGGGAACTGGGTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACt                                                                                            <  1:1209185/98‑1 (MQ=25)
            aTGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCt                                                                                         >  1:3775641/1‑100 (MQ=255)
            aTGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCt                                                                                         <  2:3401031/100‑1 (MQ=255)
            aTGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCt                                                                                         >  1:4286585/1‑100 (MQ=255)
              gtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTg                                                                                        >  1:2983940/1‑99 (MQ=255)
              gtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGc                                                                                       >  1:869788/1‑100 (MQ=255)
                ggAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAActgctgat                                                                                      <  1:2090242/99‑3 (MQ=38)
                 gAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCTgatgt                                                                                    <  2:3192283/100‑5 (MQ=37)
                   aTGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCt                                                                                  <  2:3051969/100‑1 (MQ=255)
                     gTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTc                                                                                >  1:3630762/1‑100 (MQ=255)
                      tCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCa                                                                               >  2:2127474/1‑100 (MQ=255)
                      tCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCa                                                                               <  2:3794126/100‑1 (MQ=255)
                        aGTGATGGAATTCAGTTTAGTGCTGCTACAAACGGCGCGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCTTTTTGACAACTTCTGCCAGCTTCACg                                                                             <  1:2050127/100‑1 (MQ=255)
                         gTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGa                                                                            >  2:4367300/1‑100 (MQ=255)
                            aTGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAg                                                                         >  2:4004916/1‑100 (MQ=255)
                             tGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAg                                                                         <  2:3553489/99‑1 (MQ=255)
                              gggATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAAGAACATCAGGAGTTGTCCAGCGCATGGACCACTTCTGCCAGCTTCACGAAAGcc                                                                       >  1:1781675/1‑100 (MQ=255)
                              gggATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGcc                                                                       <  2:3288049/100‑1 (MQ=255)
                                gATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCtt                                                                     >  1:4354721/1‑100 (MQ=255)
                                gATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCtt                                                                     >  2:1404480/1‑100 (MQ=255)
                                gATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCtt                                                                     >  1:769093/1‑100 (MQ=255)
                                  ttCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTAt                                                                   <  1:1517819/100‑1 (MQ=255)
                                   tCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTAtt                                                                  <  1:1650586/100‑1 (MQ=255)
                                   tCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTAtt                                                                  <  2:1805224/100‑1 (MQ=255)
                                    cAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTc                                                                 <  1:1470133/100‑1 (MQ=255)
                                      gTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTa                                                               >  2:2854471/1‑100 (MQ=255)
                                      gTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTa                                                               >  2:2609550/1‑100 (MQ=255)
                                      gTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTa                                                               >  2:3858884/1‑100 (MQ=255)
                                      gTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTa                                                               >  2:254189/1‑100 (MQ=255)
                                        ttAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAcc                                                             <  2:3042525/100‑1 (MQ=255)
                                        ttAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAcc                                                             <  2:789366/100‑1 (MQ=255)
                                        ttAGTGCTGCTACAAACGGCGGGAAGTGGATAAAAACAACGTCAGAAGGTGTCCAGCGCATGGACAGCTTCTGCCAGTTTCACGAGAGCCTTATTCTAcc                                                             <  1:2647342/100‑1 (MQ=255)
                                               tgctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCgg                                                      >  2:2243196/1‑100 (MQ=255)
                                               tgctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCgg                                                      >  2:2768374/1‑100 (MQ=255)
                                                gctACAAACGGCTTGAATCGGTTAAAACCAACATCTGCAATTCTCCAGCGCATGGACAACCTCTGCCCGCTGCACGAATGCCTTATACTACCTTTTCGGc                                                     <  1:94885/100‑1 (MQ=255)
                                                  tACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTg                                                   <  1:2127467/100‑1 (MQ=255)
                                                   aCAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCgg                                                      <  1:2583937/96‑1 (MQ=255)
                                                    cAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGCTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtc                                                 <  1:3157173/100‑1 (MQ=255)
                                                         ggcggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttc                                            <  2:1262439/100‑1 (MQ=255)
                                                           cggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttctc                                          >  1:447798/1‑100 (MQ=255)
                                                           cggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttctc                                          >  1:1728302/1‑100 (MQ=255)
                                                           cggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttct                                           >  2:3018336/1‑99 (MQ=255)
                                                              gAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCg                                       <  2:4194981/100‑1 (MQ=255)
                                                              gAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCg                                       <  2:4097171/100‑1 (MQ=255)
                                                               aaTTGGTTAAAAACAACATCAGAAGTTGTCCAGCGTATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGt                                      <  1:3551353/100‑1 (MQ=255)
                                                               aaTTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGt                                      >  1:2238590/1‑100 (MQ=255)
                                                                   ggTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAACCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTg                                  >  1:1629371/1‑100 (MQ=255)
                                                                    gTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGt                                 >  1:1653183/1‑100 (MQ=255)
                                                                    gTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGt                                 >  1:4075988/1‑100 (MQ=255)
                                                                    gTTAAAAACAACATCAGAAGATGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGt                                 >  2:4150919/1‑100 (MQ=255)
                                                                      tAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGttt                               >  1:1280003/1‑100 (MQ=255)
                                                                      tAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGGCTCTTCTCTCGTACTGttt                               <  1:1805368/100‑1 (MQ=255)
                                                                         aaaCAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAg                            <  1:3886966/100‑1 (MQ=255)
                                                                         aaaCAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAg                            >  1:1105074/1‑100 (MQ=255)
                                                                         aaaCAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAg                            >  1:2876063/1‑100 (MQ=255)
                                                                         aaaCAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGACAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAg                            >  1:2480681/1‑100 (MQ=255)
                                                                           acaacaTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAgat                          >  2:525797/1‑98 (MQ=255)
                                                                            caacaTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAt                         >  2:3219483/1‑100 (MQ=255)
                                                                            caacaTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAt                         <  1:4184354/100‑1 (MQ=255)
                                                                               caTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTg                      <  2:30948/100‑1 (MQ=255)
                                                                               caTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTg                      >  2:1000788/1‑100 (MQ=255)
                                                                                ngcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                     >  1:303/3‑100 (MQ=38)
                                                                                ggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                     >  2:1890081/3‑100 (MQ=38)
                                                                                ggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCATTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTGTAAGTATTTGt                     >  1:3293585/3‑100 (MQ=37)
                                                                                 gcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGtt                    >  1:1913560/2‑100 (MQ=38)
                                                                                  cAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTc                   >  2:61864/1‑100 (MQ=38)
                                                                                  cAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTc                   >  2:2458922/1‑100 (MQ=255)
                                                                                    gAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg                 >  2:758203/1‑100 (MQ=39)
                                                                                    gAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg                 >  1:4094438/1‑100 (MQ=39)
                                                                                    gAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg                 >  1:3660426/1‑100 (MQ=39)
                                                                                    gAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg                 >  2:473328/1‑100 (MQ=39)
                                                                                    gAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg                 >  2:3687443/1‑100 (MQ=39)
                                                                                    gAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCGCGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg                 >  1:2365671/1‑100 (MQ=255)
                                                                                    gAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg                 >  1:3923181/1‑100 (MQ=255)
                                                                                     aaGTTGTCCAGCGCATGGACATCTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgc                <  1:2012652/100‑1 (MQ=255)
                                                                                     aaGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgc                <  1:371922/100‑1 (MQ=255)
                                                                                      aGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgcg               >  1:598334/1‑100 (MQ=39)
                                                                                      aGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgcg               >  1:2785119/1‑100 (MQ=39)
                                                                                      aGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgcg               <  2:1632036/100‑1 (MQ=255)
                                                                                      aGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTCTAAGTATTTGTTCcgcg               >  1:2402651/1‑100 (MQ=255)
                                                                                        ttGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCt             <  2:2774708/100‑1 (MQ=255)
                                                                                          gTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTc           <  2:3598109/100‑1 (MQ=255)
                                                                                          gTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTc           >  1:3009399/1‑100 (MQ=255)
                                                                                          gTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTc           >  1:2266873/1‑100 (MQ=255)
                                                                                           tCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCa          <  2:4319828/100‑1 (MQ=255)
                                                                                           tCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCa          <  1:3231214/100‑1 (MQ=255)
                                                                                             cAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAg        >  2:3356828/1‑100 (MQ=255)
                                                                                              aGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGc       >  2:1034937/1‑100 (MQ=255)
                                                                                                 cgcATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAg     >  1:3580986/1‑100 (MQ=255)
                                                                                                    aTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGc  <  1:3303160/100‑1 (MQ=255)
                                                                                                    |                                                                                                   
CTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGC  >  NC_000913/3183251‑3183449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: