Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 120,154 Δ1 bp 19.6% intergenic (+19/+24) ampE → / ← aroP ampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913120,1540T.19.6% 606.6 / 142.9 214intergenic (+19/+24)ampE/aroPampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter
Reads supporting (aligned to +/- strand):  ref base T (84/88);  new base . (19/23);  total (103/111)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

Only 100 of 220 total aligned reads displayed.

GCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGG  >  NC_000913/120055‑120253
                                                                                                   |                                                                                                    
gCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGCTGGTGTAAAGATTATTGCCCTCACCCt                                                                                                      >  2:4210733/1‑100 (MQ=255)
     ttCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACg                                                                                                <  2:2310238/100‑1 (MQ=255)
     ttCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACg                                                                                                <  1:1840980/100‑1 (MQ=255)
      tCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGATGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACgg                                                                                               >  2:3115988/1‑100 (MQ=255)
       cAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACggg                                                                                              <  1:3115970/100‑1 (MQ=255)
          tGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGa                                                                                           <  2:1952603/100‑1 (MQ=255)
           ggCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAg                                                                                          <  1:1365979/100‑1 (MQ=255)
           ggCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAg                                                                                          <  1:58890/100‑1 (MQ=255)
           ggCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAg                                                                                          <  2:3583174/100‑1 (MQ=255)
            gCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAgg                                                                                         <  1:31755/100‑1 (MQ=255)
             cGAAGAAAGCGTCGTGCGTGGTCGTTGTGGTGATTGCAATACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAggg                                                                                        <  1:1577702/100‑1 (MQ=255)
             cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAggg                                                                                        >  2:3772812/1‑100 (MQ=255)
             cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAggg                                                                                        <  1:1864757/100‑1 (MQ=255)
             cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAggg                                                                                        <  2:3448400/100‑1 (MQ=255)
             cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTCCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAggg                                                                                        <  2:3224548/100‑1 (MQ=255)
              gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCATTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAg                                                                                          <  2:566749/97‑1 (MQ=255)
              gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAGGGc                                                                                       <  2:205161/100‑1 (MQ=255)
              gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAGGGc                                                                                       <  2:3051588/100‑1 (MQ=255)
               aagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCg                                                                                      <  2:8850/100‑1 (MQ=255)
                 gaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAGGGcaat                                                                                    <  1:3324880/100‑4 (MQ=38)
                    aaCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagag                                                                                 >  1:2829299/1‑100 (MQ=255)
                     aCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagaga                                                                                <  1:1322323/100‑1 (MQ=255)
                     aCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagaga                                                                                >  1:3479179/1‑100 (MQ=255)
                      ccTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagagag                                                                               >  2:1589022/1‑100 (MQ=255)
                      ccTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagagag                                                                               >  1:1996931/1‑100 (MQ=255)
                      ccTCGTTCGGGGTCGTGGTGGTGATTGCACTGCTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagagag                                                                               <  1:531477/100‑1 (MQ=255)
                       cTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAgagaga                                                                              <  1:925265/100‑1 (MQ=255)
                         cgttcgtGGTCGTGGTGGTGATTGCACTACTGACGATTTCCGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGAtt                                                                            >  1:655586/1‑100 (MQ=255)
                         cgttcgtGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGAtt                                                                            <  2:146030/100‑1 (MQ=255)
                          nttcgtGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTa                                                                           >  2:2240419/2‑100 (MQ=255)
                             cgtggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATg                                                                        <  2:3479202/100‑1 (MQ=255)
                             cgtggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATg                                                                        <  2:3303536/100‑1 (MQ=255)
                             cgtggtcgtggtGGTGATTGCACTACTGACGAGTTACGGTGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATg                                                                        <  1:2991105/100‑1 (MQ=255)
                                ggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATgcgc                                                                     <  1:1069604/100‑1 (MQ=255)
                                ggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATgcgc                                                                     >  1:1457742/1‑100 (MQ=255)
                                     tggtggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCttttn                                                                <  1:2240409/100‑2 (MQ=255)
                                       gtggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACg                                                              >  2:1833333/1‑100 (MQ=255)
                                        tggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACgg                                                             <  2:2513830/100‑1 (MQ=255)
                                         ggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGc                                                            <  2:2393075/100‑1 (MQ=255)
                                         ggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGc                                                            >  1:1018772/1‑100 (MQ=255)
                                         ggtgATTGCACTACTGACGATTTACGGGGCGGTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGc                                                            <  1:3061893/100‑1 (MQ=255)
                                            gATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCttt                                                         >  1:2067263/1‑100 (MQ=255)
                                               tGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGc                                                      >  1:3646690/1‑100 (MQ=255)
                                                gCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCg                                                     <  2:319432/100‑1 (MQ=255)
                                                gCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCg                                                     <  2:234968/100‑1 (MQ=255)
                                                gCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCg                                                     >  1:1165473/1‑100 (MQ=255)
                                                 cACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGACCTCACCCTGTACGGGTGAGGGCGTGGAGAGATTAATGCGCTTTTACGGCGTTGGCgg                                                    >  2:3804542/1‑100 (MQ=255)
                                                   ctactGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGtt                                                  <  2:1969327/100‑1 (MQ=255)
                                                   ctactGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGtt                                                  <  2:2849111/100‑1 (MQ=255)
                                                    ttctGACGATTTACGGGGTGTTGGTTTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGAGCTTTTACGGCTTTGGCGGt                                                   <  1:2162880/96‑1 (MQ=255)
                                                      ctGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtc                                               <  2:2590792/100‑1 (MQ=255)
                                                      ctGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtc                                               <  2:2962743/100‑1 (MQ=255)
                                                        gACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtctc                                             >  2:767980/1‑100 (MQ=255)
                                                         aCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTctct                                            <  1:4376758/100‑1 (MQ=255)
                                                           gATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCttt                                          <  2:2884890/100‑1 (MQ=255)
                                                            aTTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTa                                         >  1:3906921/1‑100 (MQ=255)
                                                            aTTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTa                                         >  1:1196122/1‑100 (MQ=255)
                                                             tttACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTaa                                        >  1:1234967/1‑100 (MQ=255)
                                                               tACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAAc                                      <  1:3389470/100‑1 (MQ=255)
                                                                aCGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACa                                     <  1:2918770/100‑1 (MQ=255)
                                                                 cGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAg                                    >  2:4088210/1‑100 (MQ=255)
                                                                  ggggCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGa                                   <  2:41712/100‑1 (MQ=255)
                                                                  ggggCGTTGGGGTAAAGATTATTGCCCTCGCCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGa                                   <  1:3925319/100‑1 (MQ=255)
                                                                   gggCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACACAt                                  >  1:2274761/1‑100 (MQ=255)
                                                                         tGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGa                            <  1:2125744/100‑1 (MQ=255)
                                                                         tGGTGTAAAGATTATTGCCCTCACCCTGGACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGa                            <  2:3733390/100‑1 (MQ=255)
                                                                          ggTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGAt                           >  1:3538640/1‑100 (MQ=255)
                                                                          ggTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGAt                           >  1:3994045/1‑100 (MQ=255)
                                                                            tgtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATAc                         <  1:3782655/100‑1 (MQ=255)
                                                                            tgtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATAc                         >  2:2103205/1‑100 (MQ=255)
                                                                            tgtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTATCTCTTTAAACAGATAGCCGATAc                         >  2:369819/1‑100 (MQ=255)
                                                                             gtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATAcc                        <  1:1952619/100‑1 (MQ=255)
                                                                             gtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATAcc                        >  2:2890634/1‑100 (MQ=255)
                                                                             gtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATAcc                        >  2:4098123/1‑100 (MQ=255)
                                                                              tAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCt                       <  2:3400173/100‑1 (MQ=255)
                                                                              tAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCt                       <  2:966870/100‑1 (MQ=255)
                                                                              tAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCt                       >  1:4279196/1‑100 (MQ=255)
                                                                               aaaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAgagatcggaag                      >  2:917186/1‑90 (MQ=255)
                                                                               aaaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTa                      <  1:124880/100‑1 (MQ=255)
                                                                               aaaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTa                      >  2:1430077/1‑100 (MQ=255)
                                                                               aaaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGCTGTCTCTTTAAACAGATAGCCGATACCTa                      >  2:2561635/1‑100 (MQ=255)
                                                                                 aGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAac                    >  2:1367733/1‑100 (MQ=255)
                                                                                  gATTATTGGCCTCACCCTGTACGGGTGAGGGCGTAGAGAGGTTAATGCGCTTTTGCGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAac                    <  1:3146372/99‑1 (MQ=255)
                                                                                   attattGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAacac                  <  2:2919343/100‑1 (MQ=255)
                                                                                        tGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCa             <  2:2770576/100‑1 (MQ=255)
                                                                                        cgCCCTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCa             >  1:997772/2‑100 (MQ=39)
                                                                                         gCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAg            >  1:2465118/1‑100 (MQ=255)
                                                                                         gCCCTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAg            >  1:3084554/1‑100 (MQ=255)
                                                                                          cccTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc           >  2:349326/1‑100 (MQ=255)
                                                                                          cccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc           >  2:3644635/1‑100 (MQ=255)
                                                                                          cccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc           >  2:1068910/1‑100 (MQ=255)
                                                                                          cccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc           >  1:2761107/1‑100 (MQ=255)
                                                                                          cccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc           >  2:2181607/1‑100 (MQ=255)
                                                                                           ccTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGcc          >  1:406744/1‑100 (MQ=255)
                                                                                             tCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCAt        >  2:3898737/1‑100 (MQ=255)
                                                                                             tCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCAt        >  1:1342194/1‑100 (MQ=255)
                                                                                              cACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATa       <  2:1165477/100‑1 (MQ=255)
                                                                                                cccTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATAcc     >  2:2582020/1‑100 (MQ=255)
                                                                                                  cTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCgg   >  2:1263163/1‑100 (MQ=255)
                                                                                                  cTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCgg   <  2:1656207/100‑1 (MQ=255)
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GCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGG  >  NC_000913/120055‑120253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: