Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,950,966:1 +C 16.1% coding (135/567 nt) yecD → isochorismatase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,950,9651.C16.1% 622.6 / 129.7 192coding (134/567 nt)yecDisochorismatase family protein
Reads supporting (aligned to +/- strand):  ref base . (77/84);  new base C (19/12);  total (96/96)
Fisher's exact test for biased strand distribution p-value = 2.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.21e-01

Only 100 of 201 total aligned reads displayed.

TGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGT  >  NC_000913/1950866‑1951064
                                                                                                    |                                                                                                   
  gtgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTcgcg                                                                                                   <  1:2544065/100‑1 (MQ=255)
  gtgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTcgcg                                                                                                   >  2:511109/1‑100 (MQ=255)
   tgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCgcgc                                                                                                  >  2:218486/1‑100 (MQ=255)
   tgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCgcgc                                                                                                  >  2:2556751/1‑100 (MQ=255)
      tCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAg                                                                                               <  1:4213100/100‑1 (MQ=255)
      tCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAg                                                                                               <  1:820434/100‑1 (MQ=255)
      tCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGCGAAATTTCGCCGCCAg                                                                                               <  1:1830563/100‑1 (MQ=255)
       cGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGc                                                                                              <  2:1041344/100‑1 (MQ=255)
       cGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGCGAAATTTCGCCGCCAg                                                                                               <  2:408929/99‑1 (MQ=255)
       cGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGCGAAATTTCGCCGCCAGc                                                                                              <  1:4383512/100‑1 (MQ=255)
       cGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGCGAAATTTCGCCGCCAGc                                                                                              <  2:3245599/100‑1 (MQ=255)
       cGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGCGAAATTTCGCCGCCAGc                                                                                              <  2:3348482/100‑1 (MQ=255)
        gATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGCGAAATTTCGCCGCCAGc                                                                                              <  1:3126745/99‑1 (MQ=39)
         aTTTACAAGAAGGCATCTTACCGTTTGCCGGTGGGCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCGGGCGGCGAAATTTCGCGCCAGCgg                                                                                            <  1:2466661/100‑1 (MQ=255)
          tttACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGt                                                                                           <  2:1396591/100‑1 (MQ=255)
           ttACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTc                                                                                          <  2:24641/100‑1 (MQ=255)
             aCAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAg                                                                                        >  1:3653673/1‑100 (MQ=255)
              cAAGGAGGCAGCTTACCTTTTGCCGGTGGTCGACATACTGCGGATGATGTAGGTAAGCGCGCCGGGAAGGTGGCGGCGAAATTTCGCGCCAGCGGTCAGc                                                                                       <  1:903642/100‑1 (MQ=255)
              cAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGc                                                                                       >  1:1236135/1‑100 (MQ=255)
              cAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGc                                                                                       >  2:2009411/1‑100 (MQ=255)
               tagaagGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTTATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGcc                                                                                      <  1:2438853/99‑1 (MQ=255)
                agaagGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGccc                                                                                     >  2:632814/1‑100 (MQ=255)
                agaagGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGcca                                                                                     >  2:2438864/1‑99 (MQ=255)
                   agGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCgtg                                                                                  >  2:1175129/1‑100 (MQ=255)
                   agGCATCGTACCTTTTGCCGGTGCTCCACATACGGCCGATGAGGTAGTTAATCGCGCCGGGAAGCGGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCgtg                                                                                  <  2:1841680/100‑1 (MQ=255)
                         cTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTg                                                                            >  1:1961521/1‑100 (MQ=255)
                          ttACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTgg                                                                           >  2:1973364/1‑100 (MQ=255)
                          ttACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTgg                                                                           <  2:1500282/100‑1 (MQ=255)
                          ttACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTgg                                                                           >  2:1095252/1‑100 (MQ=255)
                          ttACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTgg                                                                           <  2:3642802/100‑1 (MQ=255)
                          ttACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTgg                                                                           <  2:3725370/100‑1 (MQ=255)
                           tACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTggn                                                                          <  1:3556680/100‑2 (MQ=255)
                           tACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGt                                                                          <  2:2235610/100‑1 (MQ=255)
                           tACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGt                                                                          >  2:2819308/1‑100 (MQ=255)
                            aCCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTg                                                                         >  2:1653051/1‑100 (MQ=255)
                             ccTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAACCCGTGGTTCTGGTgc                                                                        >  1:3615427/1‑100 (MQ=255)
                              cTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTgcg                                                                       >  2:864192/1‑100 (MQ=255)
                              cTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTgcg                                                                       <  1:2191334/100‑1 (MQ=255)
                                tttGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGcgcg                                                                     >  2:459666/1‑100 (MQ=255)
                                 ttGCCGGTGGTCCACATACTTCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGcgcg                                                                     <  1:1652883/99‑1 (MQ=255)
                                  tGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGtt                                                                   >  1:4407796/1‑100 (MQ=255)
                                  tGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGtt                                                                   >  1:1894789/1‑100 (MQ=255)
                                   gCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGttn                                                                  <  2:838180/100‑2 (MQ=255)
                                   gCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTg                                                                  >  2:3865085/1‑100 (MQ=255)
                                   gCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTg                                                                  >  2:3955030/1‑100 (MQ=255)
                                   gCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTg                                                                  <  1:2972840/100‑1 (MQ=255)
                                         ggtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGt                                                            <  2:785756/100‑1 (MQ=255)
                                          gtCCAGATACGGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTGCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTc                                                           <  1:3752640/100‑1 (MQ=255)
                                          gtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGt                                                            <  1:986419/99‑1 (MQ=255)
                                          gtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTc                                                           <  2:4326072/100‑1 (MQ=255)
                                          gtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTc                                                           <  2:2397025/100‑1 (MQ=255)
                                            ccACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCt                                                          >  1:1063038/1‑99 (MQ=255)
                                            ccACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTg                                                         <  1:1183544/100‑1 (MQ=255)
                                               caTACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCg                                                      <  2:1431894/100‑1 (MQ=255)
                                                 tACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGAt                                                    >  2:4392574/1‑100 (MQ=255)
                                                 tACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGAt                                                    >  1:2419574/1‑100 (MQ=255)
                                                   cTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTa                                                  >  2:1154403/1‑100 (MQ=255)
                                                   cTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTa                                                  <  1:2259067/100‑1 (MQ=255)
                                                    tGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTAc                                                 <  1:1095249/100‑1 (MQ=255)
                                                     gCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGAGTTGGCTGGTCTGCCGATTa                                                  >  1:1432773/1‑98 (MQ=255)
                                                        gATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTg                                             >  2:1698281/1‑100 (MQ=255)
                                                              gTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCAt                                       <  1:652135/100‑1 (MQ=255)
                                                               tAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCAtt                                      >  1:3395338/1‑100 (MQ=255)
                                                                aGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGTGCCAGCGGTCAGCCCGTGTTTCTGGTGCGAGTTGGCTGGTCTGCCGTTTACGCTGAAg                                          <  1:390841/95‑1 (MQ=255)
                                                                 gTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTaa                                    <  1:521305/100‑1 (MQ=255)
                                                                 gTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTaa                                    >  1:2497930/1‑100 (MQ=255)
                                                                    aaTCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGACGCATTaaaa                                  >  2:4129271/1‑99 (MQ=255)
                                                                    aaTCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAAc                                 >  1:3495289/1‑100 (MQ=255)
                                                                    aaTCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAAc                                 >  2:1821252/1‑100 (MQ=255)
                                                                     aTCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACa                                >  2:3810692/1‑100 (MQ=255)
                                                                       cgcgCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGc                              >  1:309522/1‑100 (MQ=255)
                                                                        gcgcCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGcc                             >  2:2883601/1‑100 (MQ=255)
                                                                         cgcCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCg                            <  2:2417541/100‑1 (MQ=255)
                                                                           gctgaccgCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGt                          >  1:269396/8‑100 (MQ=37)
                                                                           ccGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGt                          >  1:769209/1‑100 (MQ=255)
                                                                           ccGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGt                          <  1:1329100/100‑1 (MQ=255)
                                                                             gggAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCg                        <  1:545526/100‑1 (MQ=255)
                                                                             gggAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCg                        <  2:2342332/100‑1 (MQ=255)
                                                                              ggAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGa                       <  2:2701271/100‑1 (MQ=255)
                                                                               accgCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCCTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGAt                      >  1:2354835/4‑100 (MQ=38)
                                                                                ccgCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATg                     >  2:2541479/3‑100 (MQ=39)
                                                                                 cgCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATgc                    >  1:2874537/2‑100 (MQ=39)
                                                                                  gCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATgcg                   >  1:2066698/1‑100 (MQ=255)
                                                                                  gCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATgcg                   >  2:4220884/1‑100 (MQ=255)
                                                                                  gCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATgcg                   >  2:2095893/1‑100 (MQ=255)
                                                                                   cTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATgcgc                  <  2:2634459/100‑1 (MQ=255)
                                                                                    tGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGcc                 >  2:3283864/1‑100 (MQ=255)
                                                                                     ggcggcGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGccc                >  2:4242313/1‑100 (MQ=255)
                                                                                      ncggcgAAATTTCGCGCCAGCGGTCGGCCGGTGTTTATGGTGCGCGTTGGCTGGTCTCCCGATCACGCTTAAGCATTAAAACAGCCTGTCGATgcgc                  >  1:1706225/2‑97 (MQ=255)
                                                                                      gcggcgCACTTTCGCGCCGGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGCTGCATTAAAACAGCCGGTCGATGCGcc                 <  1:4292816/98‑1 (MQ=255)
                                                                                        ggcgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGACGCATTAAAACAGCCGGTCGATGAGccat               >  2:3806137/1‑96 (MQ=255)
                                                                                          cgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCAcc           >  2:3757131/1‑100 (MQ=255)
                                                                                          cgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCAcc           >  2:4114185/1‑100 (MQ=255)
                                                                                          cgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCAcc           >  2:4292140/1‑100 (MQ=255)
                                                                                          cgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCAcc           >  1:2603017/1‑100 (MQ=255)
                                                                                             aaTTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGc        >  2:3064330/1‑100 (MQ=255)
                                                                                             aaTTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGc        >  1:2003188/1‑100 (MQ=255)
                                                                                             aaTTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGc        >  1:1037445/1‑100 (MQ=255)
                                                                                               tttCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTa      >  2:2006722/1‑100 (MQ=255)
                                                                                                   gcgcCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGT  <  1:857186‑M1/100‑1 (MQ=255)
                                                                                                    |                                                                                                   
TGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGT  >  NC_000913/1950866‑1951064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: