Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,652,855:1 (G)6→7 100% coding (362/846 nt) sseA → 3‑mercaptopyruvate sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,652,8491.G95.4% 450.2 / 11.1 131coding (356/846 nt)sseA3‑mercaptopyruvate sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base . (6/0);  new base G (53/72);  total (59/72)
Fisher's exact test for biased strand distribution p-value = 7.21e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

AGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑GGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAAT  >  NC_000913/2652750‑2652943
                                                                                                    |                                                                                                 
aGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCt                                                                                                    >  2:4382194/1‑100 (MQ=255)
  gATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑gg                                                                                                <  1:3641842/100‑1 (MQ=255)
    tAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑gggg                                                                                             <  1:539703/100‑1 (MQ=255)
    tAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑gggg                                                                                             >  2:3108849/1‑100 (MQ=255)
    tAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑gggg                                                                                             <  1:537751/100‑1 (MQ=255)
      aGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑gggggg                                                                                          >  2:3595166/1‑100 (MQ=255)
       gCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑ggggggg                                                                                         >  1:1717835/1‑99 (MQ=255)
        cACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑ggggggg                                                                                         >  1:3112112/1‑98 (MQ=255)
         aCCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑gggggggtg                                                                                       >  1:4013319/1‑97 (MQ=255)
         aCCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑gggggggtg                                                                                       <  2:3051914/100‑4 (MQ=255)
         aCCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑gggggggtg                                                                                       <  1:2115195/100‑4 (MQ=255)
         aCCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑gggggggtg                                                                                       <  2:3151597/100‑4 (MQ=255)
          ccTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑gggggggtgg                                                                                      <  2:2936503/100‑5 (MQ=255)
           cTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑ggggggggtgg                                                                                     <  2:3963097/100‑6 (MQ=255)
             gATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACt                                                                                    <  2:1431878/100‑1 (MQ=255)
                 gTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCa                                                                                >  2:1833596/1‑100 (MQ=255)
                 gTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCa                                                                                <  2:1806477/100‑1 (MQ=255)
                 gTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCa                                                                                >  2:3929388/1‑100 (MQ=255)
                   cTATGACGAAGGTAATCTTTTCTCCGCCCCACTAGCATGGTGGAGGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCa                                                                                <  2:3576792/98‑1 (MQ=255)
                   cTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAgg                                                                              <  2:1859611/100‑1 (MQ=255)
                   cTATGACGAAGGTAATCTTTTCTCAGCACCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGTTTCTGGGGGGGTGGACTTGCAg                                                                               >  2:2352631/1‑99 (MQ=255)
                   cTATGACGAAGGTAATCTTTGCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAg                                                                               <  1:1845053/99‑1 (MQ=255)
                    tATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGc                                                                             >  2:2547273/1‑100 (MQ=255)
                    tATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGc                                                                             <  1:3438803/100‑1 (MQ=255)
                     aTGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCt                                                                            <  2:1651688/100‑1 (MQ=255)
                      tGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTg                                                                           >  1:3343639/1‑100 (MQ=255)
                       gACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTgg                                                                          >  1:3650640/1‑100 (MQ=255)
                       gACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTgg                                                                          <  2:3029347/100‑1 (MQ=255)
                        aCGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTgagatcggaa                                                                         >  1:926933/1‑91 (MQ=255)
                        aCGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGc                                                                         <  2:2597008/100‑1 (MQ=255)
                        aCGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGc                                                                         <  2:3833614/100‑1 (MQ=255)
                        aCGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGc                                                                         <  2:2090305/100‑1 (MQ=255)
                         cGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCa                                                                        >  2:2692489/1‑100 (MQ=255)
                          gAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAg                                                                       <  2:2893428/100‑1 (MQ=255)
                          gAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAg                                                                       <  2:3459349/100‑1 (MQ=255)
                             ggTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAgcgc                                                                    >  2:4121460/1‑100 (MQ=255)
                              gTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGcgcg                                                                   <  2:3525581/100‑1 (MQ=255)
                               tAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGcgcg                                                                   >  1:933917/1‑99 (MQ=255)
                                 aTCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCaagatcgga                                                                >  1:3863190/1‑92 (MQ=255)
                                 aTCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgatg                                                                >  2:3287887/1‑100 (MQ=255)
                                 aTCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgatg                                                                >  2:1499636/1‑100 (MQ=255)
                                   cTTTTCTCAGCCCCCCGAGCAAGGTGGATGCTGCGCACCTTTGGAGTAGAGAAAGTGTCGACTATGGGGGGGTAGACTTGCGGCATGCCAGCGTggtgat                                                              >  1:182397/1‑100 (MQ=255)
                                    ttttCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATc                                                             <  2:3202118/100‑1 (MQ=255)
                                    ttttCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATc                                                             <  2:2691457/100‑1 (MQ=255)
                                     tttCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATct                                                            >  1:1712803/1‑100 (MQ=255)
                                     tttCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGGGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATct                                                            <  1:3140613/100‑1 (MQ=255)
                                      ttCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATttg                                                           >  1:1887394/1‑100 (MQ=255)
                                      ttCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATctg                                                           >  2:1607686/1‑100 (MQ=255)
                                       tctcAGCGCCACGAGCATGGTGGATGCTGCGCACCTTGGGTGTAGAGAAAGTGTCGATTCTGGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATct                                                            <  1:2728461/99‑1 (MQ=255)
                                       tctcAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATctgc                                                          <  2:3266835/100‑1 (MQ=255)
                                       tctcAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATctgc                                                          >  1:3399145/1‑100 (MQ=255)
                                        ctcAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATctgct                                                         >  1:4341870/1‑100 (MQ=255)
                                        ctcAGCCCCACGAGCATGGTGGATGCTGCGCACCCCTGGCGCAGAGAAAGTGTCGAACCT‑‑GGAGGGGTGGACTTGCAGGCGGGCAGCGCGATAAActg                                                           >  1:1465334/1‑98 (MQ=255)
                                         tcAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAACGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATctgctg                                                        >  1:3537053/1‑100 (MQ=255)
                                         tcAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATctgctg                                                        <  1:3963133/100‑1 (MQ=255)
                                         tcAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATctgctg                                                        >  1:1258355/1‑100 (MQ=255)
                                         tcAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATctgctg                                                        <  2:3897770/100‑1 (MQ=255)
                                          cAGCCCCACGAGCATGGTGGATGCTGCGCACCTGTGGTGTAGAGAAAGTGTCGATTCT‑‑GGGGGAGTGGACTTGCAGGCTTGCAGCGCGATGATCTGCTGt                                                       >  1:1717968/1‑100 (MQ=255)
                                           aGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGtt                                                      <  2:3690084/100‑1 (MQ=255)
                                            gCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGtt                                                      <  2:2001877/100‑1 (MQ=255)
                                            gCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGtt                                                      >  1:2337543/1‑99 (MQ=255)
                                             ccccACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTag                                                    <  2:3628601/100‑1 (MQ=255)
                                              cccACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTaga                                                   >  2:3846985/1‑100 (MQ=255)
                                               ccACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTagaa                                                  >  1:3190689/1‑100 (MQ=255)
                                                  cGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAgaagaa                                               >  2:864905/1‑100 (MQ=255)
                                                    aGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAgg                                             <  2:1156560/100‑1 (MQ=255)
                                                    aGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAgg                                             <  1:4121430/100‑1 (MQ=255)
                                                    aGCATGGTGGATGCTGCGCACCGTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTTCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAgg                                             <  1:2347666/100‑1 (MQ=255)
                                                     gCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGt                                            <  1:4281069/100‑1 (MQ=255)
                                                      cATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTg                                           >  2:71537/1‑100 (MQ=255)
                                                        tggtggATGCTGCGCACCGTTGGTGTAGAGAAAGGGCCGAGTCTGGGGGGGTGGACTTGCGGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGc                                          <  1:2731554/99‑1 (MQ=255)
                                                         ggtggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAgg                                             >  1:257801/1‑95 (MQ=255)
                                                         ggtggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAg                                        >  1:4377200/1‑100 (MQ=255)
                                                         ggtggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAg                                        >  2:4123591/1‑100 (MQ=255)
                                                          gtggATGCTGCGCACCTTTGGTGTAGCGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGt                                       <  2:3512388/100‑1 (MQ=255)
                                                           tggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTa                                      <  1:3846957/100‑1 (MQ=255)
                                                           tggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTa                                      <  1:2195320/100‑1 (MQ=255)
                                                              aTGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTagag                                   <  1:739574/100‑1 (MQ=255)
                                                                gctgcGCACCGTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGGTGTTCTGCTGTTAGAAGAAGGTGCAGTAGAGCt                                 <  1:2902689/100‑1 (MQ=255)
                                                                 ctgcGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTg                                >  1:1209076/1‑100 (MQ=255)
                                                                        ccTTTGGTGTAGAGAAAGTGGCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGCTCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAg                         <  1:2714517/100‑1 (MQ=255)
                                                                         cTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAgg                        <  1:3703133/100‑1 (MQ=255)
                                                                         cTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAgg                        <  2:822112/100‑1 (MQ=255)
                                                                           ttGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGgag                      <  1:2215930/100‑1 (MQ=255)
                                                                           ttGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGgag                      <  1:1349699/100‑1 (MQ=255)
                                                                           ttGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGgag                      <  1:3529278/100‑1 (MQ=255)
                                                                             ggTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGgagan                    <  2:3779443/100‑2 (MQ=255)
                                                                             ggTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGagag                    <  1:2036156/100‑1 (MQ=255)
                                                                              gtgtAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGt                   >  2:3891056/1‑100 (MQ=255)
                                                                              gtgtAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGt                   <  2:3370289/100‑1 (MQ=255)
                                                                               tgtAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGtt                  <  2:3192082/100‑1 (MQ=255)
                                                                                   gagaAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAAc              <  2:3547860/100‑1 (MQ=255)
                                                                                   gagaAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAAc              >  2:4255320/1‑100 (MQ=255)
                                                                                   gagaAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAAc              >  1:722277/1‑100 (MQ=255)
                                                                                    agaAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAAcg             <  2:1339515/100‑1 (MQ=255)
                                                                                        aGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAAcgccgc         <  2:2120773/100‑1 (MQ=255)
                                                                                        aGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAAcgccgc         >  1:4291570/1‑100 (MQ=255)
                                                                                           gtCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGtt      >  1:3764659/1‑100 (MQ=255)
                                                                                           gtCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGtt      <  2:1258359/100‑1 (MQ=255)
                                                                                            tCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGttt     <  1:1586625/100‑1 (MQ=255)
                                                                                                    |                                                                                                 
AGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑GGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAAT  >  NC_000913/2652750‑2652943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: