Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,446,357:1 +T 15.4% intergenic (+151/+21) ydbL → / ← feaR DUF1318 family protein/transcriptional activator for tynA and feaB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,446,3571.T15.4% 440.7 / 86.0 136intergenic (+151/+21)ydbL/feaRDUF1318 family protein/transcriptional activator for tynA and feaB
Reads supporting (aligned to +/- strand):  ref base . (61/54);  new base T (6/15);  total (67/69)
Fisher's exact test for biased strand distribution p-value = 5.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.87e-01

Only 100 of 141 total aligned reads displayed.

TTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCA  >  NC_000913/1446258‑1446456
                                                                                                    |                                                                                                   
ttttCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCa                                                                                                      <  1:2686587/100‑1 (MQ=255)
ttttCCTAAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCa                                                                                                      <  2:3957284/100‑1 (MQ=255)
 tttCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAg                                                                                                    >  2:1305166/1‑100 (MQ=255)
    ccTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTAc                                                                                                 >  2:726461/1‑100 (MQ=255)
     cTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACa                                                                                                >  1:2376203/1‑100 (MQ=255)
      tGAACCAAGGTGAGTATTCAAAACCCCCTGTTAAATTGCCTTTTGCGCTGAAAAGTGCAGAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACAt                                                                                               <  1:1802402/100‑1 (MQ=255)
      tGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACAt                                                                                               >  1:4108386/1‑100 (MQ=255)
       gAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATCGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATg                                                                                              >  1:793755/1‑100 (MQ=255)
         aaCAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCa                                                                                            <  1:851527/100‑1 (MQ=255)
             aGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGc                                                                                        >  1:1712597/1‑100 (MQ=255)
              ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCATATATCTGTTTTAACTAATTGGCGTTGCATTACATGCAACgtc                                                                                       <  2:2972689/100‑3 (MQ=255)
              ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGcc                                                                                       <  2:3605598/100‑1 (MQ=38)
              ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGcc                                                                                       <  1:2471556/100‑1 (MQ=38)
              ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGcc                                                                                       <  1:2006214/100‑1 (MQ=38)
              ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCGACGcc                                                                                       <  1:4109107/100‑1 (MQ=255)
              ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGcc                                                                                       >  1:2874687/1‑100 (MQ=255)
              ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGcc                                                                                       <  2:1694770/100‑1 (MQ=255)
              ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGcc                                                                                       <  2:3269075/100‑1 (MQ=255)
               gggAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCa                                                                                      <  2:627356/98‑1 (MQ=38)
               gTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCa                                                                                      <  2:133600/100‑1 (MQ=255)
               gTGAATATTCAAAAACTCCTGTATAATTGCAATAAGAACAGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGcc                                                                                       <  1:1069855/99‑1 (MQ=16)
                tGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCaa                                                                                     <  2:1234444/100‑1 (MQ=38)
                tGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCaa                                                                                     >  2:1143477/1‑100 (MQ=255)
                tGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCaa                                                                                     <  1:3674764/100‑1 (MQ=255)
                  aaTATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAtt                                                                                   >  1:1192165/1‑100 (MQ=255)
                   atatTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCAAtta                                                                                  <  2:3374410/100‑1 (MQ=38)
                   atatTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCAAtta                                                                                  <  1:2470970/100‑1 (MQ=38)
                    tatTCAAAAACTCCTGTCAAATTGCCTGTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttag                                                                                 <  1:1576766/100‑1 (MQ=255)
                     atTTAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCAAttagt                                                                                <  1:3901543/100‑1 (MQ=37)
                     atTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCAAttagt                                                                                <  2:675491/100‑1 (MQ=37)
                      ttCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagtt                                                                               <  1:2631029/100‑1 (MQ=255)
                         aaaaaCTCCTGTCAAATTGTCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagtt                                                                               <  1:3496499/97‑1 (MQ=255)
                         aaaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAATTATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGc                                                                            <  1:638133/100‑1 (MQ=255)
                         aaaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGc                                                                            >  1:3266178/1‑100 (MQ=255)
                         aaaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGc                                                                            >  2:70946/1‑100 (MQ=255)
                          aaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCg                                                                           <  2:2101654/100‑1 (MQ=255)
                          aaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCg                                                                           >  1:3346901/1‑100 (MQ=255)
                           aaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCAAttagttaaaac                                                                          <  2:1120710/100‑5 (MQ=25)
                            cgatcttGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGa                                                                         <  2:2647805/94‑1 (MQ=255)
                               tCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTTTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAAtt                                                                      >  1:2629728/1‑100 (MQ=255)
                                 cTGTCAAATTGCATTTTGAACTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTa                                                                    <  1:2788781/100‑1 (MQ=255)
                                  tGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTAc                                                                   >  1:3378282/1‑100 (MQ=255)
                                   gTCAAATTGCCTTTTGCGCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACg                                                                  <  1:2931400/100‑1 (MQ=255)
                                    tCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGt                                                                 <  2:1524287/100‑1 (MQ=255)
                                     cAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTc                                                                <  2:3378302/100‑1 (MQ=255)
                                     cAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTc                                                                <  1:2423861/100‑1 (MQ=255)
                                     cAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGAAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTc                                                                >  1:2140175/1‑100 (MQ=255)
                                       aaTTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGa                                                              >  2:2408362/1‑100 (MQ=255)
                                       aaTGGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTAGCGTTGAAGTACATGCAACGCCAATTAGTTAGCGGAATTTAAGTCg                                                               >  1:1918297/1‑99 (MQ=255)
                                        aTTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGAt                                                             <  1:381595/100‑1 (MQ=255)
                                             attttGCCCTGGCTAATGCATAGTGGCATCTCTGTTTTAACTAATTGGCGTTGCAGTACTTTCAGCACGAACTAGTACTCGAAATGTACGTGGCTACTCg                                                        >  1:1668689/2‑100 (MQ=255)
                                               tttGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGcc                                                      <  1:3609559/100‑1 (MQ=255)
                                               tttGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGc                                                       <  2:1312116/99‑1 (MQ=255)
                                                 tGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTg                                                    >  1:4248921/1‑100 (MQ=255)
                                                  gCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTgg                                                   >  2:3527683/1‑100 (MQ=255)
                                                    ccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCg                                                 <  2:2425386/100‑1 (MQ=255)
                                                    ccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCg                                                 >  2:591932/1‑100 (MQ=255)
                                                     cTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGt                                                <  2:2346522/100‑1 (MQ=255)
                                                     cTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGACTGGCGt                                                >  1:670231/1‑100 (MQ=255)
                                                          aaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATcc                                           >  1:1487297/1‑100 (MQ=255)
                                                           aaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATccc                                          >  1:503504/1‑100 (MQ=255)
                                                           aaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATcc                                           <  2:1463897/99‑1 (MQ=255)
                                                               cATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAg                                      >  1:280185/1‑100 (MQ=255)
                                                               cATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAg                                      >  2:4062690/1‑100 (MQ=255)
                                                                 tAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCg                                    >  2:1160475/1‑100 (MQ=255)
                                                                   gTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAGGCGtt                                  <  1:2281289/100‑1 (MQ=255)
                                                                        atatCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTa                             <  2:127407/100‑1 (MQ=255)
                                                                        atatCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTa                             >  2:640930/1‑100 (MQ=255)
                                                                          atCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTaaa                           >  2:2786056/1‑100 (MQ=255)
                                                                           tCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAAt                          <  2:411147/100‑1 (MQ=255)
                                                                            cTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATa                         >  2:3045668/1‑100 (MQ=255)
                                                                             tGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAc                        >  1:2224495/1‑100 (MQ=255)
                                                                              gTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAcc                       >  2:1627871/1‑100 (MQ=255)
                                                                              gTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAcc                       >  1:3822418/1‑100 (MQ=255)
                                                                                tttAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGt                     >  1:203147/1‑100 (MQ=255)
                                                                                 ttAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTGAATACCGtt                    >  2:1422783/1‑100 (MQ=255)
                                                                                  tAACTAATTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTg                   <  1:942765/100‑1 (MQ=37)
                                                                                  tAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTg                   <  2:3664719/100‑1 (MQ=255)
                                                                                  tAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTg                   >  1:1135892/1‑100 (MQ=255)
                                                                                   aaCTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGa                  >  2:3326940/1‑100 (MQ=255)
                                                                                      naaTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAAGTTACGTCGATACTCGCCTGGCTTCATACCATAGCGTTGCTTACATACCTTTGaaaa               >  1:3271499/2‑100 (MQ=255)
                                                                                       aaTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaaaa              >  2:2363108/1‑100 (MQ=255)
                                                                                        aTTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAAt             >  2:2848097/1‑100 (MQ=38)
                                                                                        aTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAAt             <  2:4166086/100‑1 (MQ=255)
                                                                                        aTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAAt             <  2:58493/100‑1 (MQ=255)
                                                                                           ggCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc          >  2:749994/1‑100 (MQ=38)
                                                                                           ggCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc          >  1:1174086/1‑100 (MQ=38)
                                                                                           ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc          >  2:3553918/1‑100 (MQ=255)
                                                                                           ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc          <  2:3719721/100‑1 (MQ=255)
                                                                                           ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc          >  2:507872/1‑100 (MQ=255)
                                                                                             cGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGActc        >  1:1143067/1‑100 (MQ=255)
                                                                                             cGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGActc        <  1:726460/100‑1 (MQ=255)
                                                                                              gTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGActct       >  1:79288/1‑100 (MQ=255)
                                                                                                tGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTgg     <  2:3505125/100‑1 (MQ=255)
                                                                                                 gCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGt    >  2:170366/1‑100 (MQ=255)
                                                                                                 gCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGt    <  1:3045650/100‑1 (MQ=255)
                                                                                                 gCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGt    >  2:2151628/1‑100 (MQ=255)
                                                                                                  cAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGt    >  2:1818252/1‑99 (MQ=255)
                                                                                                  cAGTACATGCAACGCCAATTAGGTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTc   <  2:3491087/100‑1 (MQ=255)
                                                                                                   aGTACATGCAACGCCAATTAGTTAGCGGGATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCa  >  2:750795/1‑100 (MQ=255)
                                                                                                    |                                                                                                   
TTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCA  >  NC_000913/1446258‑1446456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: