New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4599652 =132 (0.840)7 (0.050) 6/164 NT 5.4% intergenic (‑211/+43) opgB/yjjA OPG periplasmic biosynthetic phosphoglycerol transferases I (membrane‑bound) and II (soluble)/putative DUF2501 family periplasmic protein
?NC_000913 4599685 = 125 (0.860)intergenic (‑244/+10) opgB/yjjA OPG periplasmic biosynthetic phosphoglycerol transferases I (membrane‑bound) and II (soluble)/putative DUF2501 family periplasmic protein
Rejected: Frequency below cutoff threshold.

GAAAAGGTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4599757‑4599652
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑acgcggcGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCACCTTTTCTGCCAGCGGAGTTGTTCCGATGTTAT  >  NC_000913/4599685‑4599783
                                                                                                                                                                                                             
GAAAAGGTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGA                                                                                                           >  2:3461307/1‑100
 AAAAGGTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGAC                                                                                                          <  2:2103265/100‑1
  AAAGGTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACG                                                                                                         <  1:3649429/100‑1
      GTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGC                                                                                                     <  1:1871301/100‑1
      GTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGC                                                                                                     >  1:2456493/1‑100
      GTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGC                                                                                                     >  2:237347/1‑100
                                   AAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGC                                                                        >  2:3924433/1‑100
                                                                CCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGTCTTT                                           >  2:3554046/1‑100
                                                                  GCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGG                                         <  1:3924403/100‑1
                                                                      AACGCCGCGTTTTAGCTGTTACCCGGCTGACGCCGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAATACCAGCTCGCAGGCTTTGGTTTT                                     >  1:307734/1‑100
                                                                       ACGCCGCGTTTTAGCTGTTACCCGGCTGAGGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTC                                    >  1:573608/1‑100
                                                                         GCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCGTTTTGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCAC                                  >  1:1880836/1‑100
                                                                         GCCGCGTTTTAGCTGTTACCCGGCTGACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCAC                                  >  2:2088310/1‑100
                                                                                                   ACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCACCTTTTCTGCCAGCGGAGTTGTTCCGA        <  2:922579/100‑1
                                                                                                   ACGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCACCTTTTCTGCCAGCGGAGTTGTTCCGA        <  2:75002/100‑1
                                                                                                    CGCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCACCTTTTCTGCCAGCGGAGTTGTTCCGAT       <  1:1217715/100‑1
                                                                                                     GCGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCACCTTTTCTGCCAGCGGAGTTGTTCCGATG      <  1:1156410/100‑1
                                                                                                      CGGCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCACCTTTTCTGCCAGCGGAGTTGTTCCGATGT     <  2:4195357/100‑1
                                                                                                        GCGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCACCTTTTCTGCCAGCGGAGTTGTTCCGATGTTA   <  1:3685498/100‑1
                                                                                                         CGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCACCTTTTCTGCCAGCGGAGTTGTTCCGATGTTAT  >  2:3364327/1‑100
                                                                                                                                                                                                             
GAAAAGGTGAAAACCAAAGCCTGCGATCTGGTGTTAAAACAGGGGCTGAACTTCATTTCCTGAACCGCGAAACGCCGCGTTTTAGCTGTTACCCGGCTGACGCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4599757‑4599652
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑acgcggcGTTTCGCGGTTCAGGAAATGAAGTTCAGCCCCTGTTTTAACACCAGATCGCAGGCTTTGGTTTTCACCTTTTCTGCCAGCGGAGTTGTTCCGATGTTAT  >  NC_000913/4599685‑4599783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.