BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,856,334	C→A	16.0%	E199* (GAG→TAG) ‡	ydjH ←	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,856,334	0	C	A	16.0%	425.1 / 62.5	161	E199* (GAG→TAG)	ydjH	putative kinase
Reads supporting (aligned to +/- strand): ref base C (65/70); new base A (14/12); total (79/82)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-01

Only 100 of 163 total aligned reads displayed.

TCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAAT  >  NC_000913/1856237‑1856433

tCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCgg                                                                                                   <  2:244566/100‑1 (MQ=255)

  tttACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCa                                                                                                 <  2:347812/100‑1 (MQ=255)

    tACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCaaa                                                                                               >  2:4344231/1‑100 (MQ=255)

      tccGTTGTAATCACCACCGTTGTTACGCCGCACGCAAGAAAGGCGTCAGCAATTTCATCCAGTGTCTCTTGCCCGGTGCGTAATTTTGCCTCGGCAAAAt                                                                                             >  1:1970481/2‑100 (MQ=255)

      cccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAAt                                                                                             <  2:1397128/100‑1 (MQ=255)

      cccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAAt                                                                                             >  2:487177/1‑100 (MQ=255)

      cccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTCTGCCTCGGCAAAAt                                                                                             >  1:3009521/1‑100 (MQ=255)

       ccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAAtt                                                                                            >  2:1769629/1‑100 (MQ=255)

       ccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAAtt                                                                                            <  1:2843945/100‑1 (MQ=255)

         gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAg                                                                                          <  1:575246/100‑1 (MQ=255)

         gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAAtt                                                                                            <  1:1322295/98‑1 (MQ=255)

          ttttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAgg                                                                                         <  2:3124244/100‑1 (MQ=255)

          ttttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAgg                                                                                         <  1:3907886/100‑1 (MQ=255)

          ttttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTact                                                                                         <  1:1577797/100‑3 (MQ=255)

          ttttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTact                                                                                         <  2:2614622/100‑3 (MQ=255)

          ttttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTact                                                                                         <  2:1131434/100‑3 (MQ=255)

           tttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGa                                                                                        <  2:1319440/100‑1 (MQ=255)

           tttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGa                                                                                        <  2:1588393/100‑1 (MQ=255)

           tttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTactc                                                                                        <  1:1479548/100‑4 (MQ=255)

           tttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTactc                                                                                        <  1:491835/100‑4 (MQ=255)

             tAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGaaa                                                                                      <  2:3009539/100‑1 (MQ=255)

             tAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTactcac                                                                                      <  1:1129363/100‑6 (MQ=39)

              aaTCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGaaan                                                                                     <  1:2607994/100‑2 (MQ=255)

              aaTCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAAc                                                                                     <  2:3687986/100‑1 (MQ=255)

               aTCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTactcaccg                                                                                    >  2:4289557/1‑93 (MQ=255)

                tCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAg                                                                                   <  1:3908786/100‑1 (MQ=255)

                tCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAg                                                                                   >  2:2542217/1‑100 (MQ=255)

                tCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTactcaccgg                                                                                   <  2:1510069/100‑9 (MQ=38)

                 caccacCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGa                                                                                  >  2:2381359/1‑100 (MQ=255)

                   ccaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAACTTAGGAAACAGATa                                                                                >  1:4324850/1‑100 (MQ=255)

                   ccaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATa                                                                                >  2:591368/1‑100 (MQ=255)

                       cGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCg                                                                            <  2:2790898/100‑1 (MQ=255)

                       cGTTTTTACGCCGCACGCAAGAAAGCAGGCAGCAATTTCATCCAGTGTCTCTTTCCCGGAGGGTACTTTTGCATCGGCAAAATTATGCAACAGATAAt                                                                              >  1:2957088/1‑98 (MQ=255)

                        gTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGa                                                                           >  2:1824619/1‑100 (MQ=255)

                         tttttACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGAc                                                                          >  2:3073977/1‑100 (MQ=255)

                          ttttACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACa                                                                         >  1:1310935/1‑100 (MQ=255)

                            ttACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAAGTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACa                                                                         <  1:2283798/98‑1 (MQ=255)

                              aCGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAAc                                                                     <  1:3140350/100‑1 (MQ=255)

                                gccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTc                                                                   <  1:1005125/100‑1 (MQ=255)

                                gccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTc                                                                   >  2:731973/1‑100 (MQ=255)

                                 ccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCTGTGAGTAATTTTGCCTCGGCCAAATTAGGAAACAGATAATCGACATAACTCa                                                                  >  2:167118/1‑100 (MQ=255)

                                    cacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATg                                                               <  1:1101457/100‑1 (MQ=255)

                                    cacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATg                                                               >  2:411585/1‑100 (MQ=255)

                                      cgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAACATTAGGAAACAGATAATCGACATAACTCAATGCt                                                             >  1:2323145/1‑100 (MQ=255)

                                       gcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCtt                                                            <  2:398942/100‑1 (MQ=255)

                                          aGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGc                                                         >  2:651795/1‑100 (MQ=255)

                                                cAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAatat                                                   <  2:2013229/100‑1 (MQ=255)

                                                 aGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatc                                                  >  1:1183482/1‑100 (MQ=255)

                                                  gTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatca                                                 <  2:76975/100‑1 (MQ=255)

                                                   tCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatcat                                                >  1:1719079/1‑100 (MQ=255)

                                                    cAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatcatc                                               >  1:342614/1‑100 (MQ=255)

                                                    cAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatcatc                                               >  2:2097039/1‑100 (MQ=255)

                                                    cAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatcatc                                               >  1:3122006/1‑100 (MQ=255)

                                                    cAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatcatc                                               >  2:2772311/1‑100 (MQ=255)

                                                     aGCAATTTCATCCAGTGTCTCTTTCCCGGTGGGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATcc                                              <  2:164751/100‑1 (MQ=255)

                                                      gCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCa                                             >  2:322259/1‑100 (MQ=255)

                                                      gCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCa                                             >  1:172838/1‑100 (MQ=255)

                                                         aTTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCg                                          >  2:460568/1‑100 (MQ=255)

                                                              aTCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttca                                     >  2:1930661/1‑100 (MQ=255)

                                                               tCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcat                                    <  2:4017370/100‑1 (MQ=255)

                                                               tCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcat                                    >  1:565032/1‑100 (MQ=255)

                                                                ccAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcatt                                   <  2:4027212/100‑1 (MQ=255)

                                                                ccAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcatt                                   <  2:107801/100‑1 (MQ=255)

                                                                ccAGTGTCACTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcatt                                   <  1:1926384/100‑1 (MQ=255)

                                                                  aGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcattca                                 >  2:1913490/1‑100 (MQ=255)

                                                                  aGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcattca                                 >  1:3509957/1‑100 (MQ=255)

                                                                      tctcGTACCCGGTGAGTAATTTTGCATCTCCAAAATTAGGAAACAGATAATCGATATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAAc                               <  1:3975591/98‑1 (MQ=255)

                                                                       ctctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAAc                               <  1:1552515/97‑1 (MQ=255)

                                                                         ctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCgg                          >  1:2751122/1‑100 (MQ=255)

                                                                         ctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCgg                          >  2:1031600/1‑100 (MQ=255)

                                                                           ttCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGtt                        <  2:2209193/100‑1 (MQ=255)

                                                                           ttCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGtt                        >  2:2657894/1‑100 (MQ=255)

                                                                            tCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGttt                       <  2:2011013/100‑1 (MQ=255)

                                                                               tgtttcctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCCATGCTTCGCAAACACCATCCAGCGTTTCATTCAACCGCAGttt                       >  1:377512/8‑97 (MQ=25)

                                                                               cGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGAt                    <  2:888182/100‑1 (MQ=255)

                                                                                ggTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTg                      <  1:2861278/97‑1 (MQ=255)

                                                                                ggTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATc                   <  2:3122024/100‑1 (MQ=255)

                                                                                ggTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATc                   <  1:1019007/100‑1 (MQ=255)

                                                                                 tttcctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCa                  >  1:669288/6‑100 (MQ=39)

                                                                                   tcctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCata                >  2:4090929/4‑100 (MQ=255)

                                                                                   gAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCata                >  1:2032272/1‑100 (MQ=255)

                                                                                    aGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCatat               >  1:3825488/1‑100 (MQ=255)

                                                                                     tccgatctGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCatatn              <  1:3477294/93‑2 (MQ=255)

                                                                                     gTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATc              >  2:583882/1‑100 (MQ=255)

                                                                                     gTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCACAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATc              >  2:2038995/1‑100 (MQ=255)

                                                                                     ctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCATTGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCatat               >  1:3741210/2‑99 (MQ=255)

                                                                                     ctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATc              >  2:1291289/2‑100 (MQ=255)

                                                                                     ctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATc              >  2:3052891/2‑100 (MQ=255)

                                                                                     ctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATc              >  1:2599539/2‑100 (MQ=255)

                                                                                       aaTTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCgg            >  2:1592292/1‑100 (MQ=255)

                                                                                        aTTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGc           <  1:216702/100‑1 (MQ=255)

                                                                                          tttGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGca          <  1:3015337/99‑1 (MQ=255)

                                                                                          gttGCCTCGGCAAAATTAGGAAACAGATACTCGACATAACTCAATGCTTCGCAAATATCATACAGCGTTTCATTCAACCGCGGTTTGATCata                >  1:3720382/2‑93 (MQ=255)

                                                                                             gCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATcagatcgga      >  2:3477317/1‑92 (MQ=255)

                                                                                             gCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGTcaca        >  1:3872340/1‑98 (MQ=255)

                                                                                             gCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGa      <  1:3034771/100‑1 (MQ=255)

                                                                                              ccTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGAt     <  1:710781/100‑1 (MQ=255)

                                                                                               cTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATa    <  2:446778/100‑1 (MQ=255)

                                                                                                 cGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAat  >  1:4284359/1‑100 (MQ=255)

                                                                                                 cGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAat  >  2:2766383/1‑100 (MQ=255)

TCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAAT  >  NC_000913/1856237‑1856433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`