Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 256,454 C→G 16.1% intergenic (+19/‑73) gpt → / → frsA xanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase/fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913256,4540CG16.1% 504.9 / 76.7 185intergenic (+19/‑73)gpt/frsAxanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase/fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
Reads supporting (aligned to +/- strand):  ref base C (77/78);  new base G (16/14);  total (93/92)
Fisher's exact test for biased strand distribution p-value = 8.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 186 total aligned reads displayed.

GTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAAC  >  NC_000913/256355‑256552
                                                                                                   |                                                                                                  
 ttgATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACt                                                                                                   >  1:3725014/1‑100 (MQ=255)
    atatCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGcc                                                                                                >  2:3491460/1‑100 (MQ=255)
    atatCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGcc                                                                                                <  1:2926108/100‑1 (MQ=255)
    atatACCGCAAGATACCTAGATTGAACAGCCGTTGTATATGGGAGTCGTATTCGTCCCGCCAATCTCCGTTCACTAATCTTTTCAACGCCTGACACTGcc                                                                                                <  1:742205/100‑1 (MQ=255)
     tatCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCTTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCg                                                                                               <  1:4156002/100‑1 (MQ=255)
     tatCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGcc                                                                                                >  1:1207106/1‑99 (MQ=255)
       tCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCggg                                                                                             <  1:3553170/100‑1 (MQ=255)
       tCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCggg                                                                                             <  1:683795/100‑1 (MQ=255)
        cccTCAAGATACCTGGTTTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTg                                                                                                  <  1:279221/94‑1 (MQ=255)
        cccGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGc                                                                                            >  1:2087660/1‑100 (MQ=255)
         ccGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCg                                                                                           <  2:3066130/100‑1 (MQ=255)
         ccGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCg                                                                                           >  1:3801602/1‑100 (MQ=255)
         ccGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCg                                                                                           <  2:3887564/100‑1 (MQ=255)
         ccGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCg                                                                                           <  2:2233912/100‑1 (MQ=255)
           gCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCgtt                                                                                         <  2:3748806/100‑1 (MQ=255)
           gCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCgtt                                                                                         <  2:2324690/100‑1 (MQ=255)
            cAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCgttg                                                                                        >  2:1159000/1‑100 (MQ=255)
            cAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCgttg                                                                                        <  2:2370328/100‑1 (MQ=255)
             aaGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCgttga                                                                                       <  2:523217/100‑2 (MQ=39)
             aaGATACCCGGATTCACCAGCCGTGGGATATGGGCGTCGTATCCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCgttgt                                                                                       <  1:1914208/100‑1 (MQ=255)
              aGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCgttgaa                                                                                      <  2:1411732/100‑3 (MQ=38)
               gATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCTCTGCCGGGCGTTGTTc                                                                                     >  2:124645/1‑100 (MQ=255)
                 tACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCgttgaaaag                                                                                   <  1:915176/100‑6 (MQ=38)
                 tACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATCCGGCCCGCCAATCTCCGGTCGCTAATATTTTCAACGCCCGGCAGTGCCAGGCgttgaaaa                                                                                    <  1:427839/99‑5 (MQ=37)
                    cTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCttttt                                                                                >  2:1795990/1‑100 (MQ=255)
                    cTGGATTGAACAGCCGTGGGATACGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCttttt                                                                                <  2:2259829/100‑1 (MQ=255)
                       gATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAAc                                                                             <  2:4210003/100‑1 (MQ=255)
                       gATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAAc                                                                             <  1:1026651/100‑1 (MQ=255)
                       gATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAAc                                                                             <  1:2079534/100‑1 (MQ=255)
                       gATTGAACAGCCGTGGGATATGGGCGTCGTATGCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAAc                                                                             <  1:1943664/100‑1 (MQ=255)
                        aTTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACt                                                                            <  2:2375968/100‑1 (MQ=255)
                        aTTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACt                                                                            <  2:2794786/100‑1 (MQ=255)
                          tgcaCAGCCGTGGGATATGGGCGTAGTATTCGTCCCGCCAATATCCGGTCGCTTATCTTTTCTACTCCTGGCACTGCCGGTCGTTGTTCTGTTTatc                                                                             <  1:1462844/94‑3 (MQ=255)
                          tGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTc                                                                          <  2:117420/100‑1 (MQ=255)
                           gAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCa                                                                         >  1:1717806/1‑100 (MQ=255)
                            aaCAGCCGTGGGAGATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTAGCACTGCAGGGCGTTGTTCTTTTTAACTTCAg                                                                        >  2:2673880/1‑100 (MQ=255)
                                gCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCgg                                                                    >  2:2042155/1‑100 (MQ=255)
                                   gTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGtt                                                                 >  1:1243327/1‑100 (MQ=255)
                                   gTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGtt                                                                 <  1:2553557/100‑1 (MQ=255)
                                   gTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGtt                                                                 >  1:1838997/1‑100 (MQ=255)
                                   gTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGt                                                                  >  1:2911160/1‑99 (MQ=255)
                                        atatGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAAt                                                            <  1:1573124/100‑1 (MQ=255)
                                         tatGGGCGTCGTATTCGTCCCGCCAATCTCCGGTAGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATa                                                           >  1:176531/1‑100 (MQ=255)
                                          ttGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAg                                                          <  1:3769141/99‑1 (MQ=255)
                                           tGGGCGTCGTATTCGTCCCGCCAATCTCCGGCCGATAATCTTTTCAACGCCTGGCAGTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAga                                                         >  2:3769167/1‑99 (MQ=255)
                                            gggCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGtt                                                        <  2:4339928/100‑1 (MQ=255)
                                             ggCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGttt                                                       >  2:3794267/1‑100 (MQ=255)
                                             ggCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGttt                                                       >  2:3072242/1‑100 (MQ=255)
                                             ggCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGttt                                                       >  2:1338084/1‑100 (MQ=255)
                                                gtcgtATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTcc                                                     <  2:702244/99‑1 (MQ=255)
                                                ggcgtATTCGTCCCGCCGATCTCCTGTCGCTAATCTTTTCAACGCCTGTCACTGCCGGGCGTTGTTC‑‑TTTGACTTCAGGCGGGTTACAATAGTTTcc                                                     <  1:1642593/95‑1 (MQ=255)
                                                 tcgtATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCag                                                   <  2:413436/100‑1 (MQ=255)
                                                     aTTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagtaag                                               <  1:1349219/100‑1 (MQ=255)
                                                     aTTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagtaag                                               <  1:4095810/100‑1 (MQ=255)
                                                     aTTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGACGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagtaag                                               <  1:3059873/100‑1 (MQ=255)
                                                       tCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATACTTTCCagtaagta                                             <  2:2997654/100‑1 (MQ=255)
                                                           cccGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCt                                         >  2:1739269/1‑100 (MQ=255)
                                                            ccGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTg                                        >  2:3261746/1‑100 (MQ=255)
                                                             cGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTgg                                       <  2:726411/100‑1 (MQ=255)
                                                               ccAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAg                                     <  2:3962850/100‑1 (MQ=255)
                                                                cAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAgg                                    >  2:4228959/1‑100 (MQ=255)
                                                                  aTCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCt                                  <  2:1935717/100‑1 (MQ=255)
                                                                     tcCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCa                               <  1:4359183/100‑1 (MQ=255)
                                                                      ccGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCAt                              >  2:1927902/1‑100 (MQ=255)
                                                                      ccGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCAt                              >  1:3425724/1‑100 (MQ=255)
                                                                      ccGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCAt                              >  1:3112901/1‑100 (MQ=255)
                                                                       cGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATc                             <  1:2299658/100‑1 (MQ=255)
                                                                        ggTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATcc                            >  1:984170/1‑100 (MQ=255)
                                                                          tCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCAt                          <  1:278038/100‑1 (MQ=255)
                                                                           cGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATg                         <  1:3406230/100‑1 (MQ=255)
                                                                           cGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATg                         <  2:513431/100‑1 (MQ=255)
                                                                           cGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATg                         >  1:1702201/1‑100 (MQ=255)
                                                                           cGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTGTCCAGTAAGTATTCTGGAGGCTGCATCCATg                         >  2:1301403/1‑100 (MQ=255)
                                                                              tAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGaca                      <  2:1457223/100‑1 (MQ=255)
                                                                               aaTCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGacac                     >  2:2505250/1‑100 (MQ=255)
                                                                                aTCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGAcaca                    >  1:101279/1‑100 (MQ=255)
                                                                                 tCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGacagat                   >  2:3682484/1‑97 (MQ=255)
                                                                                 tCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAg                   >  2:2019167/1‑100 (MQ=255)
                                                                                 aaagaacAACGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAg                   >  1:461184/7‑100 (MQ=37)
                                                                                  cTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACATTAGTTTCCCGTAAGTATTCTGGAGGCTGCATCAATGACACAgg                  >  1:3644854/1‑100 (MQ=255)
                                                                                  cTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAgg                  >  2:4053060/1‑100 (MQ=255)
                                                                                  cTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAgg                  <  2:1856427/100‑1 (MQ=255)
                                                                                  cTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAgg                  >  1:2206267/1‑100 (MQ=255)
                                                                                    gaacAACGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACATTAGTTTCGAGTAAGTATTCTGGAGGCTGCATCCATGACACAgg                  >  1:3953487/4‑98 (MQ=38)
                                                                                    gaacAACGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATTCCACAGGc                 >  1:490994/4‑99 (MQ=37)
                                                                                    gaacAACGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCa                >  2:1547699/4‑100 (MQ=38)
                                                                                    gaacAACGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCa                >  2:4252801/4‑100 (MQ=38)
                                                                                      tCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATTACACAGGCa                >  1:2169924/1‑98 (MQ=255)
                                                                                      tCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCaaa              <  2:3458346/100‑1 (MQ=255)
                                                                                       cAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAAc             >  2:1077265/1‑100 (MQ=255)
                                                                                       cAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAAc             >  2:2834410/1‑100 (MQ=255)
                                                                                        aaCGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAAcc            <  1:2369210/100‑1 (MQ=255)
                                                                                         aCGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCt           >  2:1482027/1‑100 (MQ=255)
                                                                                          cGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTg          <  2:1353898/100‑1 (MQ=255)
                                                                                            ccTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAg        <  1:3794240/100‑1 (MQ=255)
                                                                                               ggCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGa     <  2:3362954/100‑1 (MQ=255)
                                                                                               ggCACTGCCGGGCGTTGTTCTTGTTAACTTCAGGCGGGTTACAATCGTTTCCAGTAAGTATTCTGGATGCTGCATCCATGACACAGACAAACCTGAGCGa     >  1:597219/1‑100 (MQ=255)
                                                                                                 cACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGaaa   >  1:2854725/1‑100 (MQ=255)
                                                                                                 cACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGaaa   >  1:344004/1‑100 (MQ=255)
                                                                                                  aCTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAAc  <  2:1629647/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
GTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAAC  >  NC_000913/256355‑256552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: