BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,081,057	A→G	16.0%	V69A (GTG→GCG) ‡	sbmC ←	DNA gyrase inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,081,057	0	A	G	16.0%	386.7 / 61.1	169	V69A (GTG→GCG)	sbmC	DNA gyrase inhibitor
Reads supporting (aligned to +/- strand): ref base A (72/69); new base G (14/13); total (87/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 183 total aligned reads displayed.

ACTACACGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACC  >  NC_000913/2080958‑2081155

aCTACACGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCa                                                                                                    <  2:1594930/100‑1 (MQ=255)

 cTACACGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCac                                                                                                   >  2:2078505/1‑100 (MQ=255)

   acacGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCacga                                                                                                 >  2:969352/1‑100 (MQ=255)

   acacGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCacga                                                                                                 >  2:3601195/1‑100 (MQ=255)

    cacGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCacgac                                                                                                >  2:3236674/1‑100 (MQ=255)

    cacGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCacgac                                                                                                >  2:2415371/1‑100 (MQ=255)

    cacGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCacgac                                                                                                <  2:3399210/100‑1 (MQ=255)

    cacGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCacga                                                                                                 <  1:1420781/99‑1 (MQ=255)

      cGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACgg                                                                                              <  2:4161024/100‑1 (MQ=255)

      cGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACgg                                                                                              >  2:158341/1‑100 (MQ=255)

         gCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGgtgt                                                                                           >  1:3111243/1‑100 (MQ=255)

          cTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGTAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTc                                                                                          <  2:3111261/100‑1 (MQ=255)

          cTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTc                                                                                          >  1:2512783/1‑100 (MQ=255)

           tACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGACTGACGCCCTCAGTGTTTTCGGGAAGCGTAAACTAACCCGGCACCGTCACGAACGTGTCg                                                                                         >  1:710351/1‑100 (MQ=255)

           tACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCg                                                                                         <  2:112407/100‑1 (MQ=255)

           tACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGACACCGTCGTGACGGTGCCg                                                                                         <  2:1749983/100‑1 (MQ=255)

            aCCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCGTGACGGTGCCg                                                                                         <  1:4159925/99‑1 (MQ=255)

            aCCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCga                                                                                        >  1:112407/1‑99 (MQ=255)

            aCCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGc                                                                                        <  2:743817/100‑1 (MQ=255)

            aCCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGACACCGTCGTGACGGTGCCgg                                                                                        <  1:3837800/100‑2 (MQ=255)

             ccGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGACACCGTCGTGACGGTGCCggg                                                                                       <  1:183748/100‑3 (MQ=255)

              cGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAg                                                                                      >  2:2108147/1‑100 (MQ=255)

               gCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCGTGACGgtgccgggtt                                                                                     <  2:2793698/100‑8 (MQ=255)

               gCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGc                                                                                     >  2:4113072/1‑100 (MQ=255)

               gCCACCGCATACTGACCACCTGTAATTGCTGTCAGAATGACGCCCTCACTGTTTCCGGGAAGCGTAAAGTAACCCGGCACGGTCGTGACGGTGCCggg                                                                                       <  1:1282928/98‑3 (MQ=255)

                ccACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCg                                                                                    >  1:3422275/1‑100 (MQ=255)

                   ccGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTCAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTaa                                                                                 >  1:1682382/1‑100 (MQ=255)

                   ccGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTaa                                                                                 >  1:2448639/1‑100 (MQ=255)

                        tACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAAttttt                                                                            >  2:1352967/1‑100 (MQ=255)

                         aCTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAAtttt                                                                             <  1:1827542/98‑1 (MQ=255)

                          cTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTct                                                                          >  1:2887414/1‑99 (MQ=255)

                            gACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTcggc                                                                        <  1:3931227/100‑1 (MQ=255)

                              ccaccTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTcggcgg                                                                      >  1:3424171/1‑100 (MQ=255)

                               caccTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCggg                                                                     >  1:2986360/1‑100 (MQ=255)

                               caccTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCggg                                                                     >  2:3853793/1‑100 (MQ=255)

                                  cTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGgtgt                                                                  >  2:137301/1‑100 (MQ=255)

                                  cTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGgtgt                                                                  >  1:1050176/1‑100 (MQ=255)

                                   tGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGAGTAAAGTACCCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTcg                                                                          >  1:3458960/1‑91 (MQ=255)

                                    gTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTACAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGttt                                                                >  2:81926/1‑100 (MQ=255)

                                     tAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTc                                                               <  1:3264049/100‑1 (MQ=255)

                                          tCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCAGGTGTTTCATCTg                                                          <  1:637590/100‑1 (MQ=255)

                                           cTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTgg                                                         >  2:3587901/1‑100 (MQ=255)

                                           cTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTgg                                                         <  2:4334762/100‑1 (MQ=255)

                                            tGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGa                                                        >  2:830807/1‑100 (MQ=255)

                                             gTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTTGAAGCGTAATTTTTCGGCGGGTGTTTCATCTGGa                                                        >  1:3665800/1‑99 (MQ=255)

                                               cAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTg                                                     <  2:4370824/100‑1 (MQ=255)

                                                aGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTCATTTTTCGGCGGGTGTTTCATCTGGATTg                                                     <  1:1500268/99‑1 (MQ=255)

                                                 gAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGt                                                    >  1:2436663/1‑99 (MQ=255)

                                                  aaTGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCg                                                  <  2:2524472/100‑1 (MQ=255)

                                                  aaTGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCg                                                  <  1:579266/100‑1 (MQ=255)

                                                  aaTGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCCTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCg                                                  >  1:802423/1‑100 (MQ=255)

                                                    tGACGCCCTCTCTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTa                                                <  1:1187506/100‑1 (MQ=255)

                                                     gACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTaa                                               <  2:3116303/100‑1 (MQ=255)

                                                      aCGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACGCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAAt                                              <  1:2329393/100‑1 (MQ=255)

                                                      aCGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAAt                                              <  2:2611636/100‑1 (MQ=255)

                                                       cGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATa                                             <  2:2004997/100‑1 (MQ=255)

                                                         cccTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGa                                           >  2:2286267/1‑100 (MQ=255)

                                                          ccTCACTGTTTTCGGGAAGCGTAAAGTAACCCTGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGAc                                          >  2:2892665/1‑100 (MQ=255)

                                                          ccTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGAc                                          <  2:1194662/100‑1 (MQ=255)

                                                          ccTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGAc                                          <  1:2601736/100‑1 (MQ=255)

                                                           cTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACa                                         >  2:3859415/1‑100 (MQ=255)

                                                            tCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAg                                        <  2:2649021/100‑1 (MQ=255)

                                                                 gTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAAcc                                   <  1:3145571/100‑1 (MQ=255)

                                                                 gTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAAcc                                   >  2:4078752/1‑100 (MQ=255)

                                                                  ttttCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAAccc                                  <  2:2614574/100‑1 (MQ=255)

                                                                  ttttCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAAccc                                  <  1:986369/100‑1 (MQ=255)

                                                                   tttCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCa                                 >  1:3857000/1‑100 (MQ=255)

                                                                          aaGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTc                          <  1:103912/100‑1 (MQ=255)

                                                                            gccttaaGTAACCCGGCACAGTGACGACGCTGTCGCAGCGTAATTTTGCGGCCGGTCTTTCATCTGTATTGTCTTCATAGACAGCAACCCACTCCTTCgg                        <  1:1340847/95‑1 (MQ=255)

                                                                            gCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCgg                        <  2:2168786/100‑1 (MQ=255)

                                                                            gCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCgg                        <  2:3876853/100‑1 (MQ=255)

                                                                             cGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGc                       <  2:1860014/100‑1 (MQ=255)

                                                                              gTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGTTGTTTGTTTCATATGGCTTGTCGTAATAGACAGCAACCCACTCCTTCGGca                      >  1:4033343/1‑100 (MQ=255)

                                                                              gTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGca                      >  2:2012052/1‑100 (MQ=255)

                                                                              gTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGca                      >  2:307191/1‑100 (MQ=255)

                                                                                aaaGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGcaca                    >  2:1864864/1‑100 (MQ=255)

                                                                                 aaGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACaa                   <  2:1822025/100‑1 (MQ=255)

                                                                                  aGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAat                  >  1:4283739/1‑100 (MQ=255)

                                                                                   gTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAat                  >  1:2209740/1‑99 (MQ=255)

                                                                                    cgctgcgacACCGTCGTGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAGTAGACAGCAACCCACTCCTTCGGCACAatat                >  1:2971557/9‑100 (MQ=255)

                                                                                     gctgcgacACCGTCGTGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAatat                >  1:1076891/8‑99 (MQ=255)

                                                                                     gctgcgacACCGTCGTGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATAtt               >  2:2431339/8‑100 (MQ=255)

                                                                                     aaCCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATAtt               >  2:4227898/1‑100 (MQ=255)

                                                                                      ctgcgacACCGTCGTGACGGTGTCGCAGCGTAATTTTTCTTTGTGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATAttt              >  1:545760/7‑100 (MQ=255)

                                                                                      ctgcgacACCGTCGTGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAatatct              >  1:2669562/7‑98 (MQ=255)

                                                                                      ctgcgacACCGTCGTGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATAttt              >  1:1799128/7‑100 (MQ=255)

                                                                                      ctgcgacACCGTCGTGACGGTGCCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATAttt              >  2:4269391/7‑100 (MQ=255)

                                                                                      aCCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATAtt               >  2:2556196/1‑99 (MQ=255)

                                                                                        gcgacACCGTCGTGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATAttttt            >  1:3977699/5‑100 (MQ=255)

                                                                                         cgacACCGTCGTGACGGTGTCGCAGCGTAATTCTTCGGCGGGCGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCCCAatct                >  1:3623555/4‑93 (MQ=255)

                                                                                         cGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTg           <  2:4113975/100‑1 (MQ=255)

                                                                                         cGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTg           <  1:2176114/100‑1 (MQ=255)

                                                                                          ggCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGc          <  2:4380354/100‑1 (MQ=255)

                                                                                           gCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCt         <  2:1408386/100‑1 (MQ=255)

                                                                                              ccGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATc      >  2:3716465/1‑100 (MQ=255)

                                                                                              ccGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATc      <  1:3928533/100‑1 (MQ=255)

                                                                                               cGTTACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCt     <  1:1823588/100‑1 (MQ=255)

                                                                                                gTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTa    >  2:1059940/1‑100 (MQ=255)

                                                                                                gTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTa    >  2:1060160/1‑100 (MQ=255)

                                                                                                  cACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTAcc  >  2:3375864/1‑100 (MQ=255)

ACTACACGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACC  >  NC_000913/2080958‑2081155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`