BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,081,058	C→T	15.8%	V69M (GTG→ATG) ‡	sbmC ←	DNA gyrase inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,081,058	0	C	T	15.8%	416.4 / 53.9	169	V69M (GTG→ATG)	sbmC	DNA gyrase inhibitor
Reads supporting (aligned to +/- strand): ref base C (73/69); new base T (14/13); total (87/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 187 total aligned reads displayed.

CTACACGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCA  >  NC_000913/2080959‑2081157

cTACACGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCac                                                                                                     >  1:2796700/1‑100 (MQ=255)

cTACACGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCac                                                                                                     >  2:2078505/1‑100 (MQ=255)

  acacGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCacga                                                                                                   >  2:969352/1‑100 (MQ=255)

   cacGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCacgac                                                                                                  <  1:3848421/100‑1 (MQ=255)

   cacGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCacgac                                                                                                  >  1:3139563/1‑100 (MQ=255)

   cacGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCacga                                                                                                   <  1:1420781/99‑1 (MQ=255)

     cGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACgg                                                                                                <  2:4161024/100‑1 (MQ=255)

        gCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGgtgt                                                                                             >  2:1106950/1‑100 (MQ=255)

          tACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCg                                                                                           >  2:3380681/1‑100 (MQ=255)

          tACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGACACCGTCGTGACGGTGCCg                                                                                           <  2:1749983/100‑1 (MQ=255)

           aCCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCGTGACGGTGCCg                                                                                           <  1:4159925/99‑1 (MQ=255)

           aCCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCga                                                                                          >  1:112407/1‑99 (MQ=255)

           aCCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGc                                                                                          <  2:743817/100‑1 (MQ=255)

            ccGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGACACCGTCGTGACGGTGCCggg                                                                                         <  1:183748/100‑3 (MQ=255)

            ccGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCCCACTGTTTTCGGGAAGCGTAAAGTAACCCGACACCGTCGTGACGGTGCCgg                                                                                          <  1:3893234/99‑2 (MQ=255)

             cGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAg                                                                                        >  2:2108147/1‑100 (MQ=255)

             cGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGACACCGTCGTGACGgtgccgggt                                                                                        <  1:1812540/100‑7 (MQ=255)

              gCCACCGCATACTGACCACCTGTAATTGCTGTCAGAATGACGCCCTCACTGTTTCCGGGAAGCGTAAAGTAACCCGGCACGGTCGTGACGGTGCCggg                                                                                         <  1:1282928/98‑3 (MQ=255)

               ccACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCGTGACGgtgccgggtta                                                                                      <  2:3156562/100‑9 (MQ=255)

               ccACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCg                                                                                      <  1:3051395/100‑1 (MQ=255)

               ccACCGCATACTGACCACCTGTAAGTGCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCg                                                                                           <  1:714167/95‑1 (MQ=255)

                     cATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAAttt                                                                                <  2:2675244/100‑1 (MQ=255)

                        aCTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAAtttt                                                                               <  1:1827542/98‑1 (MQ=255)

                           gACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTcggc                                                                          <  1:3931227/100‑1 (MQ=255)

                              caccTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCggg                                                                       <  2:434007/100‑1 (MQ=255)

                              caccTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCggg                                                                       >  2:3853793/1‑100 (MQ=255)

                                 cTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGgtgt                                                                    >  2:1246222/1‑100 (MQ=255)

                                 cTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGgtgt                                                                    >  1:1050176/1‑100 (MQ=255)

                                  tctAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGtt                                                                   >  2:2990796/3‑100 (MQ=255)

                                  tGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCCCGACGGTGTCGCAGCGTGATTTTTCGGCGGGTGtt                                                                   >  2:6035/1‑100 (MQ=255)

                                  tGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGtt                                                                   <  1:590834/100‑1 (MQ=255)

                                  tGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGAGTAAAGTACCCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTcg                                                                            >  1:3458960/1‑91 (MQ=255)

                                   gTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTACAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGttt                                                                  >  2:81926/1‑100 (MQ=255)

                                   gTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGttt                                                                  >  2:2359874/1‑100 (MQ=255)

                                     aaTTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCa                                                                <  1:3708163/100‑1 (MQ=255)

                                         tCTGTCAGAATGACGCCCTCCCTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTg                                                            <  2:2618503/100‑1 (MQ=255)

                                         tCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTg                                                            <  1:3351386/100‑1 (MQ=255)

                                          cTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTgg                                                           <  2:4334762/100‑1 (MQ=255)

                                           tGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGa                                                          >  2:830807/1‑100 (MQ=255)

                                           tGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGa                                                          <  1:3993558/100‑1 (MQ=255)

                                              cAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTg                                                       >  1:3630227/1‑100 (MQ=255)

                                                gAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGt                                                      >  1:2436663/1‑99 (MQ=255)

                                                gAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTc                                                     >  2:2433559/1‑100 (MQ=255)

                                                 aaTGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCg                                                    <  2:387572/100‑1 (MQ=255)

                                                  aTGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCg                                                    <  1:2616793/99‑1 (MQ=255)

                                                  aTGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGt                                                   >  2:1370210/1‑100 (MQ=255)

                                                   tGACGCCCTCTCTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTa                                                  <  1:1187506/100‑1 (MQ=255)

                                                    gACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTaa                                                 <  2:3116303/100‑1 (MQ=255)

                                                     aCGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACGCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAAt                                                <  1:2329393/100‑1 (MQ=255)

                                                     aCGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAAt                                                <  2:2611636/100‑1 (MQ=255)

                                                          cTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACa                                           <  2:200155/100‑1 (MQ=255)

                                                          cTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACa                                           >  2:3859415/1‑100 (MQ=255)

                                                          cTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACa                                           >  2:2006213/1‑100 (MQ=255)

                                                            cACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGc                                         >  2:3093590/1‑100 (MQ=255)

                                                            cACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGc                                         <  1:1459866/100‑1 (MQ=255)

                                                            cACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGc                                         <  1:3236656/100‑1 (MQ=255)

                                                                 ttttCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAAccc                                    <  1:986369/100‑1 (MQ=255)

                                                                    tCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACt                                 <  2:3249112/100‑1 (MQ=255)

                                                                      gggAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTcc                               >  2:4091566/1‑100 (MQ=255)

                                                                      gggAACCGTAAAGTAACCCGGCACCGTGACGACGGTGTCGCAGCGTAATTTTTCGGCGGGGGTTTGCTCAGGACTGTCGTAATCaacaacaac                                      >  1:3422634/1‑93 (MQ=255)

                                                                       ggAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCt                              >  2:3403938/1‑100 (MQ=255)

                                                                         aaGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTc                            <  1:103912/100‑1 (MQ=255)

                                                                          aGCGTAAAGTTACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTCGACTGTCGTAATAGACAGCAACCCACTCCTTCg                           <  1:2893432/100‑1 (MQ=255)

                                                                          aGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCg                           <  1:248767/100‑1 (MQ=255)

                                                                          aGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCg                           <  2:1591432/100‑1 (MQ=255)

                                                                           gCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCgg                          <  2:2168786/100‑1 (MQ=255)

                                                                            cGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCgg                          <  2:2560400/99‑1 (MQ=255)

                                                                            cGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGc                         <  1:830806/100‑1 (MQ=255)

                                                                            cGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGc                         <  2:1860014/100‑1 (MQ=255)

                                                                             gTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGTTGTTTGTTTCATATGGCTTGTCGTAATAGACAGCAACCCACTCCTTCGGca                        >  1:4033343/1‑100 (MQ=255)

                                                                             gTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGca                        >  2:2430016/1‑100 (MQ=255)

                                                                             gTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGca                        >  2:2033269/1‑100 (MQ=255)

                                                                                aaGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACaa                     <  2:1822025/100‑1 (MQ=255)

                                                                                 aGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAat                    <  2:2796717/100‑1 (MQ=255)

                                                                                 aGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAat                    >  1:4283739/1‑100 (MQ=255)

                                                                                  gTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAat                    >  1:2209740/1‑99 (MQ=255)

                                                                                   cgctgcgacACCGTCGTGACGGTGTCGCAGCGTAATTTTTTTTTTTTTTTTTTATCTGGATTGTCGTAATAGTCAGCAAGTTACTCTTTTGGCACAatgt                  >  1:2597260/9‑98 (MQ=255)

                                                                                   cgctgcgacACCGTCGTGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAGTAGACAGCAACCCACTCCTTCGGCACAatat                  >  1:2971557/9‑100 (MQ=255)

                                                                                    gctgcgacACCGTCGTGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAatat                  >  1:1076891/8‑99 (MQ=255)

                                                                                    aaCCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATAtt                 >  2:4227898/1‑100 (MQ=255)

                                                                                     ctgcgacACCGTCGTGACGGTGTCGCAGCGTAATTTTTCTTTGTGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATAttt                >  1:545760/7‑100 (MQ=255)

                                                                                     ctgcgacACCGTCGTGACGGTGCCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCCAAATAttt                >  1:70785/7‑100 (MQ=255)

                                                                                     ctgcgacACCGTCGTGACGGTGCCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATAttt                >  2:4269391/7‑100 (MQ=255)

                                                                                      cccGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATAtttt               >  1:1758557/1‑100 (MQ=255)

                                                                                       gcgacACCGTCGTGACGGTGCCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATAttttt              >  1:167823/5‑100 (MQ=255)

                                                                                        cGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTg             <  2:115831/100‑1 (MQ=255)

                                                                                        cGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTg             <  1:2176114/100‑1 (MQ=255)

                                                                                        cGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTg             <  2:4113975/100‑1 (MQ=255)

                                                                                         ggCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGc            <  2:4380354/100‑1 (MQ=255)

                                                                                         cgatctgTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGc            <  2:3601190/94‑1 (MQ=255)

                                                                                          gCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCt           <  2:1408386/100‑1 (MQ=255)

                                                                                          gCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCt           <  2:2539760/100‑1 (MQ=255)

                                                                                            aCCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTAt         >  2:3915099/1‑100 (MQ=255)

                                                                                             ccGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATc        >  2:3716465/1‑100 (MQ=255)

                                                                                               gTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGcagatcg      >  1:3601165/1‑94 (MQ=255)

                                                                                               gTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTa      >  2:1059940/1‑100 (MQ=255)

                                                                                               gTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTa      >  2:146600/1‑100 (MQ=255)

                                                                                                   cgacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCca  <  1:1352962/100‑1 (MQ=255)

                                                                                                   cgacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCca  <  1:2279379/100‑1 (MQ=255)

                                                                                                   cgacgGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCca  >  1:3954940/1‑100 (MQ=255)

CTACACGAGCTACCGCCACCGCATACTGACCACCTGTAATTTCTGTCAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCA  >  NC_000913/2080959‑2081157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`