Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,323,291 T→A 17.1% intergenic (+40/‑156) atoC → / → atoD fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,323,2910TA17.1% 490.3 / 80.1 186intergenic (+40/‑156)atoC/atoDfused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (80/74);  new base A (16/16);  total (96/90)
Fisher's exact test for biased strand distribution p-value = 8.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 188 total aligned reads displayed.

AGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGC  >  NC_000913/2323192‑2323389
                                                                                                   |                                                                                                  
aGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAAt                                                                                                    >  1:3575795/1‑100 (MQ=255)
    gCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTc                                                                                                <  2:2949877/100‑1 (MQ=255)
     ccGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCt                                                                                               <  1:1676161/100‑1 (MQ=255)
     ccGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGAGGTATAACACCAAAACTTGCTATGCAGAAATTTGCACGGTGCGCAAtt                                                                                                   >  1:4408714/1‑96 (MQ=255)
      cGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTg                                                                                              >  2:41234/1‑100 (MQ=255)
      cGTGCATTGATGTATAAACTCCAGGAAGACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTg                                                                                              <  1:2328874/100‑1 (MQ=255)
       gTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGc                                                                                             <  1:1665562/100‑1 (MQ=37)
       gTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGc                                                                                             <  1:1695458/100‑1 (MQ=37)
       gTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGc                                                                                             <  2:1440018/100‑1 (MQ=25)
        tGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCa                                                                                            >  2:1095153/1‑100 (MQ=255)
        tGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCa                                                                                            <  2:4021385/100‑1 (MQ=255)
         gCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCAt                                                                                           <  1:2844824/100‑1 (MQ=25)
          cATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATa                                                                                          <  1:493473/100‑1 (MQ=255)
           aTTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAg                                                                                         <  2:4227855/100‑1 (MQ=25)
           aTTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAg                                                                                         <  2:1514298/100‑1 (MQ=25)
            ttGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGc                                                                                        <  2:3362274/100‑1 (MQ=25)
            ttGATGTATAAACTCCAGGAATACGGTAGCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGc                                                                                        <  1:1380853/100‑1 (MQ=25)
             tGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGcc                                                                                       >  2:181097/1‑100 (MQ=255)
                tGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCt                                                                                    <  2:190102/100‑1 (MQ=255)
                tGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCt                                                                                    <  2:1859006/100‑1 (MQ=255)
                tGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCt                                                                                    <  1:1038279/100‑1 (MQ=255)
                  tataAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCa                                                                                  >  2:2444339/1‑100 (MQ=255)
                   ataAACTCCAGGAATACGGTATCGATCCGGCGGATGTATATCACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCa                                                                                  >  2:2828715/1‑99 (MQ=255)
                     aaaCTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATtc                                                                               >  1:222557/1‑100 (MQ=255)
                     aaaCTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATtc                                                                               <  1:1953477/100‑1 (MQ=255)
                      aaCTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATtct                                                                              >  1:1160077/1‑100 (MQ=255)
                      aaCTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATtct                                                                              <  2:971847/100‑1 (MQ=255)
                        cTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTcc                                                                            <  1:805518/100‑1 (MQ=255)
                         tCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCt                                                                           <  2:2944017/100‑1 (MQ=255)
                         tCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCt                                                                           <  2:1649957/100‑1 (MQ=255)
                         tCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCt                                                                           <  2:2501435/100‑1 (MQ=255)
                          ccAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCtt                                                                          >  1:3690661/1‑100 (MQ=255)
                           cAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTTCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCt                                                                           <  1:2714760/98‑1 (MQ=255)
                           cAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTta                                                                         <  1:2884193/100‑1 (MQ=255)
                            aGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTtat                                                                        <  2:2875688/100‑1 (MQ=255)
                             ggAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTtata                                                                       <  2:20263/100‑1 (MQ=255)
                               aaTACTGTATCGATCCGGCGGATGTATACCACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTtat                                                                        <  1:873653/97‑1 (MQ=255)
                                   cGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATccc                                                                 <  2:3890819/100‑1 (MQ=255)
                                   cGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATcc                                                                  <  1:1768062/99‑1 (MQ=255)
                                       aTCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATc                                                             >  2:4288827/1‑100 (MQ=255)
                                        tCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATcc                                                            >  2:4247652/1‑100 (MQ=255)
                                         cGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTATGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCa                                                           >  2:3088388/1‑100 (MQ=255)
                                         agATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCa                                                           >  1:3228814/2‑100 (MQ=255)
                                          gATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCaa                                                          >  1:3268925/1‑100 (MQ=255)
                                           aTCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTACACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCGTATAAATCCCCATCCAAt                                                         <  1:389063/100‑1 (MQ=255)
                                            tCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAAtt                                                        <  1:1409818/100‑1 (MQ=255)
                                              cggcggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTa                                                      <  2:1398342/100‑1 (MQ=255)
                                               ggcggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTAt                                                     >  1:636608/1‑100 (MQ=255)
                                                gcggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATc                                                    >  2:2158028/1‑100 (MQ=255)
                                                 cggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATcc                                                   >  2:72033/1‑100 (MQ=255)
                                                     tGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATACCCATCCAATTTATCCCTTc                                               >  1:3398518/1‑100 (MQ=255)
                                                     tGTATAACACCAAAACTTGCTACGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTc                                               >  2:73283/1‑100 (MQ=255)
                                                      gTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCa                                              <  1:4236783/100‑1 (MQ=255)
                                                      gTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAACTTATCCCTTCa                                              <  2:2080754/100‑1 (MQ=255)
                                                      gTATAACACCAAAACTTGCTACGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCa                                              <  1:73283/100‑1 (MQ=255)
                                                       tataACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCat                                             >  2:415537/1‑100 (MQ=255)
                                                       tataACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCat                                             >  2:3402505/1‑100 (MQ=255)
                                                         taACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCatat                                           <  2:1400608/100‑1 (MQ=255)
                                                          aaCACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTCTCCCTTCatat                                           >  2:2569075/1‑99 (MQ=255)
                                                          aaCACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATAtt                                          >  2:2160339/1‑100 (MQ=255)
                                                           acacCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTc                                         >  2:2602022/1‑100 (MQ=255)
                                                            cacCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCa                                        >  2:546120/1‑100 (MQ=255)
                                                             acCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCaa                                       >  2:3568763/1‑100 (MQ=255)
                                                             acCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCaa                                       >  2:596128/1‑100 (MQ=255)
                                                             acCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCATTATAAATCCCCATCCAATTTATCCCTTCATATTCaa                                       >  1:185106/1‑100 (MQ=255)
                                                              ccAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAt                                      >  2:1335867/1‑100 (MQ=255)
                                                              ccAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAt                                      >  1:3818420/1‑100 (MQ=255)
                                                              ccAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAt                                      >  2:2262563/1‑100 (MQ=255)
                                                               cAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAtt                                     >  1:341303/1‑100 (MQ=255)
                                                                aaaaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAtt                                     <  1:2444328/99‑1 (MQ=255)
                                                                   aCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGtt                                 >  2:1370512/1‑100 (MQ=255)
                                                                    cTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGtt                                 <  1:3488460/99‑1 (MQ=255)
                                                                      ggCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTaaa                              >  2:814084/2‑100 (MQ=25)
                                                                      ggCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATAATCCATATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTaaa                              >  2:3023300/2‑100 (MQ=21)
                                                                       gCTATGCAGAAATTTGCACAGTGCGCACTTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATCTTCAATTAGt                                  >  1:2487840/1‑95 (MQ=255)
                                                                       gCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAAt                             >  2:3663887/1‑100 (MQ=255)
                                                                       gCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAAt                             >  2:2643602/1‑100 (MQ=25)
                                                                       gCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAAt                             >  2:1538638/1‑100 (MQ=25)
                                                                         tATGCAGAAATTTGCACAGTTCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTGCATATTCAATTAGTTAAATa                            <  1:4192920/99‑1 (MQ=255)
                                                                         tATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCGATCCACTTTATCCCCTCATATTCAATTAGTTAAATaa                           >  1:2971193/1‑100 (MQ=21)
                                                                           tGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACt                         >  2:3698283/1‑100 (MQ=255)
                                                                           tGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACt                         >  2:2986864/1‑100 (MQ=25)
                                                                            gCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCTCCATCCAATTTATCCCTTCATATTCAATTAGTTCAATAACTa                        >  1:507336/1‑100 (MQ=25)
                                                                            gCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTAATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTa                        >  2:918746/1‑100 (MQ=25)
                                                                            gCAGAAAATTGCGCACTGTGAAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCACCATCCAATTTACCCATTCATATTCAATTAGTCAAATAACTa                        >  1:2073822/1‑100 (MQ=21)
                                                                             cAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTaa                       >  1:2151082/1‑100 (MQ=255)
                                                                               gAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAAt                     >  2:3252124/1‑100 (MQ=255)
                                                                                 aaTTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATcc                   <  1:1064427/100‑1 (MQ=255)
                                                                                   tttGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCaa                 <  1:1513439/100‑1 (MQ=255)
                                                                                   tttGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTAACCCTTCATATTCAATTAGCTAAATAACTAAATCCaa                 >  2:3856554/1‑100 (MQ=255)
                                                                                    ttGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCaat                >  1:1961062/1‑100 (MQ=255)
                                                                                    ttGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCaat                <  1:596126/100‑1 (MQ=255)
                                                                                       cacaGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCaataat             <  1:104805/100‑1 (MQ=255)
                                                                                         caGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAAtct           >  1:4086174/1‑100 (MQ=255)
                                                                                         caGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAAtct           <  2:2726467/100‑1 (MQ=255)
                                                                                              cgcAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTc      >  1:4324264/1‑100 (MQ=255)
                                                                                                cAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTg    >  2:1602301/1‑100 (MQ=255)
                                                                                                 aaTTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTgg   >  2:2543035/1‑100 (MQ=255)
                                                                                                  aTTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGc  <  1:1207051/100‑1 (MQ=255)
                                                                                                  aTTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGc  <  1:472621/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
AGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGC  >  NC_000913/2323192‑2323389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: