Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,483,900 T→A 17.5% V49D (GTC→GAC)  evgA → response regulator in two‑component regulatory system with EvgS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,483,9000TA17.5% 385.8 / 69.0 160V49D (GTC→GAC) evgAresponse regulator in two‑component regulatory system with EvgS
Reads supporting (aligned to +/- strand):  ref base T (71/61);  new base A (14/14);  total (85/75)
Fisher's exact test for biased strand distribution p-value = 8.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 172 total aligned reads displayed.

TTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTA  >  NC_000913/2483801‑2483998
                                                                                                   |                                                                                                  
  cGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGtca                                                                                                  >  2:4105546/1‑100 (MQ=255)
  cGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGtca                                                                                                  >  2:3396763/1‑100 (MQ=255)
  cGTAATTGAGTGATCAAAAACGATATTGAAATCTTAGTAGAGTTTACTGAAGGCGGAAGTGCCCTTCAGCGGGTGGAAACACTTAAGCCTGATATCGt                                                                                                    <  1:1104922/98‑1 (MQ=255)
    tAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGtcatc                                                                                                <  2:3080148/100‑1 (MQ=255)
         tATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGa                                                                                           >  2:1220753/1‑100 (MQ=255)
         tATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGa                                                                                           >  1:4335510/1‑100 (MQ=255)
         tATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGa                                                                                           <  1:4020486/100‑1 (MQ=255)
         tATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGa                                                                                           <  1:190513/100‑1 (MQ=255)
           ttaagcAAATACGATATTGAAATCTTAGCAGAGTTTACTGAATGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTTAGCCTGATATCGTCATCATTGATg                                                                                         <  1:4021592/95‑1 (MQ=255)
           ttGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAAAACTTAAGCCTGATATCGTCATCATTGATg                                                                                         >  1:4306957/1‑100 (MQ=255)
            tGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGt                                                                                        >  1:627451/1‑100 (MQ=255)
            tGAGCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCTGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGt                                                                                        <  1:480606/100‑1 (MQ=255)
             gATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTc                                                                                       >  2:1121331/1‑100 (MQ=255)
             gATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTc                                                                                       >  1:4170350/1‑100 (MQ=255)
             gATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTc                                                                                       >  2:570329/1‑100 (MQ=255)
              aTCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCg                                                                                      >  1:283132/1‑100 (MQ=255)
               tCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGa                                                                                     >  1:653952/1‑100 (MQ=255)
               tCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGa                                                                                     >  1:1373463/1‑100 (MQ=255)
               tCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGa                                                                                     <  1:1450513/100‑1 (MQ=255)
                cAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGat                                                                                    >  2:1985138/1‑100 (MQ=255)
                cAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGat                                                                                    >  1:3696387/1‑100 (MQ=255)
                cAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGat                                                                                    >  2:44491/1‑100 (MQ=255)
                 aaaaaCGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGata                                                                                   <  1:1733791/100‑1 (MQ=255)
                 aaaaaCGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGata                                                                                   <  2:2777764/100‑1 (MQ=255)
                  aaaaCGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGatat                                                                                  >  2:188921/1‑100 (MQ=255)
                  aaaaCGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGatat                                                                                  <  2:600341/100‑1 (MQ=39)
                   aaaCGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATc                                                                                 <  1:2659585/100‑1 (MQ=39)
                      cGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcagg                                                                              <  1:2072330/100‑4 (MQ=38)
                      cGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcagg                                                                              <  2:2374495/100‑4 (MQ=38)
                      cGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcag                                                                               <  2:1466193/99‑3 (MQ=38)
                       gATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCg                                                                             >  1:3020944/1‑100 (MQ=255)
                       gATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcaggc                                                                             <  2:3578902/100‑5 (MQ=38)
                       gATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcaggc                                                                             <  2:3429799/100‑5 (MQ=38)
                       gATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcagg                                                                              <  1:4120332/99‑4 (MQ=38)
                        atatTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCgg                                                                            <  1:3141465/100‑1 (MQ=255)
                        atatTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCgg                                                                            <  2:2018603/100‑1 (MQ=255)
                        atatTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcaggct                                                                            <  2:580523/100‑6 (MQ=37)
                          atTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAg                                                                          <  2:3984148/100‑1 (MQ=255)
                            tGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGGTATCGTCATCATTGATGTCGATATCCCCGGAGtt                                                                        >  1:2325059/1‑100 (MQ=255)
                              aaaTCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTaa                                                                      >  1:2251376/1‑100 (MQ=255)
                               aaTCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAAc                                                                     <  1:398226/100‑1 (MQ=255)
                               aaTCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAAc                                                                     <  2:3573491/100‑1 (MQ=255)
                                 tCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCCGCGGGTGGAAACCCATAAGCCTGATATCCTCATCATTGCTGTCGATATtccacgagctga                                                                      >  1:4357082/1‑88 (MQ=255)
                                  cTTATCAGAATGAACCGAAGCCTGAAGTGCCGTTTAGCTTTGGCAAACACTTAAGCCTGATATCGTCATTATTGACGTCGCGACCCCTGGAGTTAACgg                                                                   <  1:175868/99‑1 (MQ=255)
                                  cTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGt                                                                  >  2:2165840/1‑100 (MQ=255)
                                      gCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATGGATGTCGAAATCCCCGGAGTTAACGGTATcc                                                              >  1:2975756/1‑100 (MQ=255)
                                          agTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgt                                                          <  1:2157843/100‑1 (MQ=255)
                                          agTTGACTGAAGGCGGAAGTGCCGTTCAGCGAGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgt                                                          <  1:4236509/100‑1 (MQ=255)
                                           gTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtg                                                         <  2:3253235/100‑1 (MQ=255)
                                           gTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtg                                                         >  1:566126/1‑100 (MQ=255)
                                            ttGACTGAAGGCGGAAGTGCCGTTCAGCGTGTGGAAACACTTAAGCCTTATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtgt                                                        <  1:2020132/100‑1 (MQ=255)
                                               aCTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAg                                                     <  2:975425/100‑1 (MQ=255)
                                               aCTGAAGGCGGAAGCGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAg                                                     >  2:4264924/1‑100 (MQ=255)
                                                cTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGa                                                    >  1:223560/1‑100 (MQ=255)
                                                 tGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGaa                                                   <  1:1493715/100‑1 (MQ=255)
                                                  gAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATACAGGTGTTAGaaa                                                  >  1:1445479/1‑100 (MQ=255)
                                                   aaGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAAc                                                 <  1:2285511/100‑1 (MQ=255)
                                                    aGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACg                                                >  2:3330080/1‑100 (MQ=255)
                                                     ggCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGc                                               <  2:134253/100‑1 (MQ=255)
                                                     ggCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGc                                               >  2:3203123/1‑100 (MQ=255)
                                                             tGCGGTTGAGCGGGTGGAAACACTTAATCCGGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAg                                           <  2:3997176/96‑1 (MQ=255)
                                                             tGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAg                                       >  2:3622349/1‑100 (MQ=255)
                                                              gCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgc                                      >  1:1707150/1‑100 (MQ=255)
                                                              gCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgc                                      >  2:384804/1‑100 (MQ=255)
                                                               ccGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgcg                                     <  1:1207209/100‑1 (MQ=255)
                                                                cGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgcgc                                    >  1:2425371/1‑100 (MQ=255)
                                                                 gTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGcc                                   <  2:693287/100‑1 (MQ=255)
                                                                 gTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGcc                                   <  2:2696772/100‑1 (MQ=255)
                                                                   tCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCaa                                 >  2:1796620/1‑100 (MQ=255)
                                                                     aGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAata                               >  1:978203/1‑100 (MQ=255)
                                                                         ngTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCACCAATATAGc                           >  2:3579285/2‑100 (MQ=255)
                                                                           tGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCgg                         <  2:2286629/100‑1 (MQ=255)
                                                                                 cacTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattat                   >  2:3357920/1‑100 (MQ=255)
                                                                                 cacTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattat                   >  1:4034341/1‑100 (MQ=255)
                                                                                   cTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattatta                 <  2:2130994/100‑1 (MQ=255)
                                                                                   cTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattatta                 <  2:3020962/100‑1 (MQ=255)
                                                                                    ttAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattattat                <  2:4269884/100‑1 (MQ=255)
                                                                                    ttAAGCCTGATATCGTCATCATTGATGTCAATATCCCCGGAGTTAACGGTATACAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattatta                 <  1:348996/99‑1 (MQ=255)
                                                                                     ctccggggATATCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattattatt               >  1:3741614/8‑100 (MQ=37)
                                                                                     ctccggggATATCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattattatt               <  2:3741640/93‑1 (MQ=37)
                                                                                       ccggggATATCGACATCAATGATGACGATATCTCCGGAGTTAACGGTATCCAGGTGTTAGAAACGATGAGGAAGCGCCAATATAGGTGAATtattattat             >  1:13924/6‑100 (MQ=25)
                                                                                       ccggggATATCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATtattattat             >  2:1078194/6‑100 (MQ=38)
                                                                                       aGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATtattattat             >  2:2223639/1‑100 (MQ=255)
                                                                                        gCCTGATATCGTCATGATAGAGGACGATATGCCCTGAGGTAACGGAAGCCAGGTGCTAGAAACGCTGAGGAAGAGACAATATAGCGGAattgtta                 >  1:1465381/1‑95 (MQ=255)
                                                                                        gCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTCTCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGAGGAattatcattatc            >  1:3496286/1‑100 (MQ=255)
                                                                                        cggggATATCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATc            >  2:3535612/5‑100 (MQ=38)
                                                                                        cggggATATCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATc            >  1:3264787/5‑100 (MQ=38)
                                                                                        cggggATATCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATc            >  2:2415965/5‑100 (MQ=38)
                                                                                         ccTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCg           <  2:2577172/100‑1 (MQ=255)
                                                                                         ccTGATATCGTCATCATTGATGTCGATATCAACGGAGTTAACGGTATCCAGGTGTTAGAAACGCAGAGGAAGCGCCAATATAGCGGAATTATTATTATc            >  1:1991469/1‑99 (MQ=255)
                                                                                           ggATATCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGACAATATAGCGGAATTATTATTATCGtc         >  1:3169344/2‑100 (MQ=38)
                                                                                           agATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGAGTTAGAAACGCTGAGGAGGCGACAATATAGTGGAATTATTATTATCg           >  1:2146136/2‑98 (MQ=255)
                                                                                            gATATCGTCATCATTAATGTCAATATCCCCGGAGTTAACGGTAACCAGGTGTTATAAACGCAAAGGAAGCGCAAATATAGCGGAATTATTATTATCGt          >  1:3618377/1‑98 (MQ=255)
                                                                                              tatCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTcc      >  1:3138656/1‑100 (MQ=255)
                                                                                              tatCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTcc      <  1:2086068/100‑1 (MQ=255)
                                                                                              tatCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGt          >  1:106585/1‑96 (MQ=39)
                                                                                              tatCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTcc      >  2:2773531/1‑100 (MQ=255)
                                                                                               atCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCg     >  1:4073365/1‑100 (MQ=255)
                                                                                                tCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGc    >  2:2277329/1‑100 (MQ=255)
                                                                                                 cGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCGCCGCt   >  2:380213/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
TTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTA  >  NC_000913/2483801‑2483998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: