Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,483,906 T→A 17.5% I51N (ATT→AAT)  evgA → response regulator in two‑component regulatory system with EvgS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,483,9060TA17.5% 379.0 / 71.8 159I51N (ATT→AAT) evgAresponse regulator in two‑component regulatory system with EvgS
Reads supporting (aligned to +/- strand):  ref base T (71/60);  new base A (14/14);  total (85/74)
Fisher's exact test for biased strand distribution p-value = 8.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.35e-01

Only 100 of 170 total aligned reads displayed.

ATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGA  >  NC_000913/2483807‑2484005
                                                                                                   |                                                                                                   
aTTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCCGCGGGTGGAAACACTTAAGCCTGATATCGTcatcat                                                                                                     <  2:3116269/100‑1 (MQ=255)
   tATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGa                                                                                                  <  1:190513/100‑1 (MQ=255)
     ttaagcAAATACGATATTGAAATCTTAGCAGAGTTTACTGAATGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTTAGCCTGATATCGTCATCATTGATg                                                                                                <  1:4021592/95‑1 (MQ=255)
     ttGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAAAACTTAAGCCTGATATCGTCATCATTGATg                                                                                                >  1:4306957/1‑100 (MQ=255)
      tGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGt                                                                                               >  1:627451/1‑100 (MQ=255)
       gATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTc                                                                                              >  2:1121331/1‑100 (MQ=255)
       gATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTc                                                                                              >  2:1167013/1‑100 (MQ=255)
        aTCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCg                                                                                             >  1:283132/1‑100 (MQ=255)
         tCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGa                                                                                            >  1:1373463/1‑100 (MQ=255)
         tCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGa                                                                                            <  1:1450513/100‑1 (MQ=255)
          cAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGat                                                                                           >  2:44491/1‑100 (MQ=255)
          cAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGat                                                                                           >  2:1985138/1‑100 (MQ=255)
          cAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGat                                                                                           <  2:2441724/100‑1 (MQ=255)
           aaaaaCGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGata                                                                                          <  1:1733791/100‑1 (MQ=255)
            aaaaCGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGatat                                                                                         >  2:188921/1‑100 (MQ=255)
            aaaaCGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGatat                                                                                         <  2:600341/100‑1 (MQ=39)
                cGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcagg                                                                                     <  2:2374495/100‑4 (MQ=38)
                cGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcagg                                                                                     <  1:2072330/100‑4 (MQ=38)
                cGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcag                                                                                      <  2:1466193/99‑3 (MQ=38)
                 gATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCg                                                                                    >  1:3020944/1‑100 (MQ=255)
                 gATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcaggc                                                                                    <  1:2282636/100‑5 (MQ=38)
                 gATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcagg                                                                                     <  1:4120332/99‑4 (MQ=38)
                  atatTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCgg                                                                                   <  2:3344098/100‑1 (MQ=255)
                    atTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAg                                                                                 <  2:3984148/100‑1 (MQ=255)
                        aaaTCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTaa                                                                             >  1:2250052/1‑100 (MQ=255)
                         aaTCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAAc                                                                            <  2:3573491/100‑1 (MQ=255)
                         aaTCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAAc                                                                            <  1:920296/100‑1 (MQ=255)
                          aTCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACg                                                                           >  1:1419162/1‑100 (MQ=255)
                           tCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCCGCGGGTGGAAACCCATAAGCCTGATATCCTCATCATTGCTGTCGATATtccacgagctga                                                                             >  1:4357082/1‑88 (MQ=255)
                           tCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACgg                                                                          >  1:355048/1‑100 (MQ=255)
                            cTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGt                                                                         <  1:3149980/100‑1 (MQ=255)
                              tAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGCGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTAt                                                                       >  1:4039336/1‑100 (MQ=255)
                                gCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATGGATGTCGAAATCCCCGGAGTTAACGGTATcc                                                                     >  1:2975756/1‑100 (MQ=255)
                                    agTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgt                                                                 <  1:2157843/100‑1 (MQ=255)
                                    agTTGACTGAAGGCGGAAGTGCCGTTCAGCGAGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgt                                                                 <  1:4236509/100‑1 (MQ=255)
                                     gTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtg                                                                >  1:566126/1‑100 (MQ=255)
                                     gTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtg                                                                <  2:3253235/100‑1 (MQ=255)
                                      ttGACTGAAGGCGGAAGTGCCGTTCAGCGTGTGGAAACACTTAAGCCTTATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtgt                                                               <  1:2020132/100‑1 (MQ=255)
                                      ttGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtgt                                                               <  2:2669135/100‑1 (MQ=255)
                                         aCTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAg                                                            <  2:975425/100‑1 (MQ=255)
                                         aCTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAg                                                            <  1:3923866/100‑1 (MQ=255)
                                           tGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGaa                                                          <  1:1493715/100‑1 (MQ=255)
                                            gAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATACAGGTGTTAGaaa                                                         >  1:1445479/1‑100 (MQ=255)
                                             aaGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAAc                                                        <  2:402061/100‑1 (MQ=255)
                                             aaGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAAc                                                        <  1:2285511/100‑1 (MQ=255)
                                               ggCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGc                                                      >  2:3526442/1‑100 (MQ=255)
                                               ggCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGc                                                      <  2:134253/100‑1 (MQ=255)
                                                gCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGca                                                     <  1:4221566/100‑2 (MQ=255)
                                                 cGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGc                                                      >  1:3630314/1‑98 (MQ=255)
                                                 cGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTg                                                    <  1:2384172/100‑1 (MQ=255)
                                                   gAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAg                                                  <  2:2834004/100‑1 (MQ=255)
                                                       tGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAg                                              >  2:3622349/1‑100 (MQ=255)
                                                       tGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAg                                              >  2:480600/1‑100 (MQ=255)
                                                        gCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgc                                             >  2:384804/1‑100 (MQ=255)
                                                        gCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgc                                             >  1:1707150/1‑100 (MQ=255)
                                                         ccGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgcg                                            <  1:1207209/100‑1 (MQ=255)
                                                           gTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGcc                                          <  1:1854042/100‑1 (MQ=255)
                                                           gTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGcc                                          <  2:2696772/100‑1 (MQ=255)
                                                             tCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCaa                                        >  2:1796620/1‑100 (MQ=255)
                                                              cAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAat                                       >  2:606770/1‑100 (MQ=255)
                                                               aGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAata                                      >  1:978203/1‑100 (MQ=255)
                                                                gCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAatat                                     >  1:3992759/1‑100 (MQ=255)
                                                                   ngTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCACCAATATAGc                                  >  2:3579258/2‑100 (MQ=255)
                                                                   ngTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCACCAATATAGc                                  >  2:3579285/2‑100 (MQ=255)
                                                                     tGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCgg                                <  2:2286629/100‑1 (MQ=255)
                                                                             cTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattatta                        <  2:2130994/100‑1 (MQ=255)
                                                                             cTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattatta                        >  2:2088909/1‑100 (MQ=255)
                                                                             cTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattatta                        <  2:3020962/100‑1 (MQ=255)
                                                                              ttAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattattat                       <  2:4269884/100‑1 (MQ=255)
                                                                               ctccggggATATCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattattatt                      >  1:3741614/8‑100 (MQ=37)
                                                                                 ccggggATATCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATtattattat                    >  2:1078194/6‑100 (MQ=38)
                                                                                 aGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATtattattat                    >  2:2223639/1‑100 (MQ=255)
                                                                                  gCCTGATATCGTCATGATAGAGGACGATATGCCCTGAGGTAACGGAAGCCAGGTGCTAGAAACGCTGAGGAAGAGACAATATAGCGGAattgtta                        >  1:1465381/1‑95 (MQ=255)
                                                                                  gCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTCTCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGAGGAattatcattatc                   >  1:3496286/1‑100 (MQ=255)
                                                                                  cggggATATCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATc                   >  1:3264787/5‑100 (MQ=38)
                                                                                  cggggATATCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATc                   >  2:3535612/5‑100 (MQ=38)
                                                                                   ggggATATCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCg                  >  2:3398150/4‑100 (MQ=38)
                                                                                   ccTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCg                  <  2:573871/100‑1 (MQ=255)
                                                                                   ccTGATATCGTCATCATTGATGTCGATATCAACGGAGTTAACGGTATCCAGGTGTTAGAAACGCAGAGGAAGCGCCAATATAGCGGAATTATTATTATc                   >  1:1991469/1‑99 (MQ=255)
                                                                                     tGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGtc                <  1:2543781/100‑1 (MQ=255)
                                                                                     ggATATCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGACAATATAGCGGAATTATTATTATCGtc                >  1:3169344/2‑100 (MQ=38)
                                                                                     agATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGAGTTAGAAACGCTGAGGAGGCGACAATATAGTGGAATTATTATTATCg                  >  1:2146136/2‑98 (MQ=255)
                                                                                      gATATCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGtct               >  2:3761201/1‑100 (MQ=39)
                                                                                        tatCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTcc             <  2:4184874/100‑1 (MQ=255)
                                                                                        tatCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTcc             <  1:44491/100‑1 (MQ=255)
                                                                                        tatCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTcc             <  1:2086068/100‑1 (MQ=255)
                                                                                        tatCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTcc             >  1:3138656/1‑100 (MQ=255)
                                                                                        tatCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGt                 >  1:106585/1‑96 (MQ=39)
                                                                                        tatCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTcc             >  2:2773531/1‑100 (MQ=255)
                                                                                         atCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCg            >  1:2225538/1‑100 (MQ=255)
                                                                                         atCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCg            >  1:4073365/1‑100 (MQ=255)
                                                                                          tCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGc           >  2:2277329/1‑100 (MQ=255)
                                                                                           cGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCGCCGCt          >  2:380213/1‑100 (MQ=255)
                                                                                                 catTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAAt    >  2:3027173/1‑100 (MQ=255)
                                                                                                 catTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAAt    >  2:2373971/1‑100 (MQ=255)
                                                                                                 catTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAAt    >  2:2162472/1‑100 (MQ=255)
                                                                                                 catTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAAt    >  1:2674968/1‑100 (MQ=255)
                                                                                                 catTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAAt    <  2:705263/100‑1 (MQ=255)
                                                                                                   ttGATGTCGGTATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGTTGCGCCAATATAGCGGAATTATTATTATCATCTCCGCTAAAAATGa  >  1:2571167/1‑100 (MQ=255)
                                                                                                   ttGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGa  <  2:323491/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
ATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGA  >  NC_000913/2483807‑2484005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: