Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,716,033 C→A 15.1% intergenic (+23/+33) eamB → / ← grcA cysteine and O‑acetylserine exporter/autonomous glycyl radical cofactor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,716,0330CA15.1% 517.2 / 71.0 191intergenic (+23/+33)eamB/grcAcysteine and O‑acetylserine exporter/autonomous glycyl radical cofactor
Reads supporting (aligned to +/- strand):  ref base C (82/79);  new base A (26/3);  total (109/82)
Fisher's exact test for biased strand distribution p-value = 6.80e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-01

Only 100 of 200 total aligned reads displayed.

TTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAG  >  NC_000913/2715934‑2716132
                                                                                                   |                                                                                                   
ttCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGc                                                                                                     <  2:1867528/100‑1 (MQ=255)
 tCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGcc                                                                                                    >  1:556374/1‑100 (MQ=255)
  cGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGccc                                                                                                   <  2:3279924/100‑1 (MQ=255)
  cGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGccc                                                                                                   <  2:1779085/100‑1 (MQ=255)
      aGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCtt                                                                                               >  1:839885/1‑100 (MQ=255)
        tATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTcn                                                                                             <  2:4108295/100‑2 (MQ=255)
        tATGGTCGCCAGTTAAATATCGTGCTTGCACTGTTGCTGGTCTATTGCGCGATACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTcc                                                                                             <  1:941046/100‑1 (MQ=255)
         aTGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCg                                                                                            >  2:1353221/1‑100 (MQ=255)
          tGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGc                                                                                           <  2:425211/100‑1 (MQ=255)
          tGGTCGCCAGGTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCg                                                                                            <  1:129239/99‑1 (MQ=255)
           ggTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCt                                                                                          <  2:103637/100‑1 (MQ=255)
           ggTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCt                                                                                          <  1:2207466/100‑1 (MQ=255)
            gTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTAAAAAAAAAAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCtt                                                                                         <  1:103681/100‑1 (MQ=255)
            gTCGCCAGTTAAATAGCGTGCTTGCCCTGTTGATGATCTATGGCGCGGTACGCATGTTCTAAAAACAAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCtt                                                                                         <  1:1845224/100‑1 (MQ=255)
             tCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGGCGACCTCTTCCGCttt                                                                                        <  2:2651786/100‑2 (MQ=255)
             tCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGGCGACCTCTTCCGCttt                                                                                        <  2:3435299/100‑2 (MQ=255)
               gCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGt                                                                                      <  1:2260419/100‑1 (MQ=255)
               gCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTa                                                                                     <  2:3619290/100‑1 (MQ=255)
                ccAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTa                                                                                     >  1:948989/1‑100 (MQ=255)
                ccAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTa                                                                                     <  1:4216393/100‑1 (MQ=255)
                  aGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAAc                                                                                   >  1:911613/1‑100 (MQ=255)
                      aaaTATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGc                                                                               <  2:3587028/100‑1 (MQ=255)
                       aaTATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCt                                                                              <  1:1353216/100‑1 (MQ=255)
                       aaTATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCt                                                                              <  2:1682815/100‑1 (MQ=255)
                        atatCGTGCTTGCCCTGTTGCTGTTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGGGGTCGCCCTCTTCCGCTTAGTAACTTGCTa                                                                             <  2:1890425/100‑1 (MQ=255)
                         tatCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTAc                                                                            <  2:1141474/100‑1 (MQ=255)
                          atCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACt                                                                           >  1:1129941/1‑100 (MQ=255)
                             gTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTaa                                                                        >  1:3392898/1‑100 (MQ=255)
                                cTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGcc                                                                     >  2:1287247/1‑100 (MQ=255)
                                cTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGcc                                                                     >  1:2109828/1‑100 (MQ=255)
                                cTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTATATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGcc                                                                     <  2:2857999/100‑1 (MQ=255)
                                  tGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCtt                                                                   >  2:2324367/1‑100 (MQ=255)
                                     ccTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACa                                                                <  1:1407603/100‑1 (MQ=255)
                                     ccTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACa                                                                <  2:664323/100‑1 (MQ=255)
                                      cTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAg                                                               <  2:3774114/100‑1 (MQ=255)
                                       tGTTGCTTGTCTATTTCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGCGGTCGCCCGCTTCCTCTTAGTAACGTGCTACTTAAGCCTTACAgg                                                              >  1:2182690/1‑100 (MQ=255)
                                       tGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAgg                                                              <  2:2600931/100‑1 (MQ=255)
                                          tGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCtt                                                           <  2:3989724/100‑1 (MQ=255)
                                           gCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCttt                                                          >  2:24964/1‑100 (MQ=255)
                                            cTGGTCTATTGGGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTc                                                         <  1:1577334/100‑1 (MQ=255)
                                            cTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTc                                                         <  2:3566012/100‑1 (MQ=255)
                                              ggTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAg                                                       >  1:3752095/1‑100 (MQ=255)
                                              ggTCTACTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAg                                                       <  2:3002373/100‑1 (MQ=255)
                                               gTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGt                                                      >  1:2601786/1‑100 (MQ=255)
                                                tCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTa                                                     <  2:3384750/100‑1 (MQ=255)
                                                 cTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTaa                                                    >  1:525540/1‑100 (MQ=255)
                                                 cTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTaa                                                    >  2:1529842/1‑100 (MQ=255)
                                                   aTTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAg                                                  >  2:2918372/1‑100 (MQ=255)
                                                   aTTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAg                                                  >  2:4079228/1‑100 (MQ=255)
                                                   aTTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCAGCTTAGTAACTTGCTACTTAAGCCTGACAGGCTTTCAGTaa                                                    >  1:2479508/1‑98 (MQ=255)
                                                    ttGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAgg                                                 <  2:2039829/100‑1 (MQ=255)
                                                      gcgcGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTa                                               >  1:3186958/1‑100 (MQ=255)
                                                        gcgGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACg                                             <  2:2870647/100‑1 (MQ=255)
                                                          ggTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTTACTTGCTACTGAAGCCTTACAGGCTTTCTGTAAAGGTACgtg                                           <  2:2977037/100‑3 (MQ=255)
                                                          ggTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTATAGGCTTTCAGTAAAGGTACGAg                                           >  1:1947566/1‑100 (MQ=255)
                                                             aCGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGa                                        >  1:4076354/1‑100 (MQ=255)
                                                               gCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATa                                      >  2:1481681/1‑100 (MQ=255)
                                                               gCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACGTGCTACTTAAGCCTTACAGGCTTTCAGAAAAGGTACGAGCGATa                                      <  1:2081701/100‑1 (MQ=255)
                                                                cagtttCTATTAACGAAAAAAAGTGGAAGAGGTCGCCCTCTTCCGGTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATa                                      <  1:3336567/96‑1 (MQ=255)
                                                                  ttttCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACg                                   >  2:1986867/1‑100 (MQ=255)
                                                                   tttCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACg                                   <  1:4255546/99‑1 (MQ=255)
                                                                       tATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTcgcg                              >  2:900388/1‑100 (MQ=255)
                                                                        aTTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCgcgc                             >  2:215015/1‑100 (MQ=255)
                                                                         ttAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCgct                            <  1:1899768/100‑1 (MQ=255)
                                                                         ttAACGAAAAAAAGCGGAAGAGGGCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCgct                            <  1:4300831/100‑1 (MQ=255)
                                                                         ntAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCgct                            >  2:828654/2‑100 (MQ=255)
                                                                          tAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCgctg                           >  2:162136/1‑100 (MQ=255)
                                                                           aaCGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCgctgc                          <  1:4163244/100‑1 (MQ=255)
                                                                           aaCGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCgctgc                          >  2:4073055/1‑100 (MQ=255)
                                                                           aaCGAAAAAAAGCGGAAGAGGGCGCCCTCATCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGCACGAGCGATAACGTCGCgctgc                          >  2:1155936/1‑100 (MQ=255)
                                                                            aCGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCgctgct                         >  1:2360835/1‑100 (MQ=255)
                                                                             cGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGctgctg                        <  2:1391975/100‑1 (MQ=255)
                                                                             agttactaaGCGGCAGAGGGCGACCACTTCCGCTCAGTAACTTGCTACTTCAGACTTCCAGGCTTTCAGTCACGGTACGAGCGATACCGTCGCgcagct                         >  1:685260/8‑99 (MQ=255)
                                                                              gAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGt                       >  2:2712753/1‑100 (MQ=255)
                                                                                 actaaGCGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTcc                    >  2:3043646/4‑100 (MQ=38)
                                                                                  ctaaGCGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCg                   >  2:3482094/3‑100 (MQ=255)
                                                                                   aaaGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCgg                  <  1:4408031/100‑1 (MQ=255)
                                                                                     aGCGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCCGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAg                >  1:3598098/1‑100 (MQ=39)
                                                                                     aGCGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCgg                  >  1:795265/1‑98 (MQ=255)
                                                                                     aGCGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAg                >  2:3254522/1‑100 (MQ=255)
                                                                                     aGCGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAg                >  2:2397584/1‑100 (MQ=255)
                                                                                      gCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACCTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGt               >  2:4187113/1‑100 (MQ=255)
                                                                                      gCGGAAGAGGGCGACCTCTTCCGCTTAGTAACTCGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGt               >  1:2651660/1‑100 (MQ=39)
                                                                                       cGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGt               >  1:209636/1‑99 (MQ=255)
                                                                                       cGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTc              >  2:1828016/1‑100 (MQ=255)
                                                                                       cGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTc              >  1:2882588/1‑100 (MQ=255)
                                                                                       cGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTc              >  2:2277652/1‑100 (MQ=255)
                                                                                        ggAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCa             >  2:31270/1‑100 (MQ=255)
                                                                                        ggAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCa             >  2:4315808/1‑100 (MQ=255)
                                                                                        ggAAGAGAGCGACCTCTTCCGCTTAGGAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGGCa             >  2:2195785/1‑100 (MQ=255)
                                                                                        ccgatctgTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCa             <  1:3656708/93‑1 (MQ=255)
                                                                                         gAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCag            <  1:174709/100‑1 (MQ=255)
                                                                                           agagGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCagag          <  1:388836/100‑1 (MQ=255)
                                                                                           agagGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGAACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCagag          <  1:2712738/100‑1 (MQ=255)
                                                                                             agGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCagagag        <  2:4045184/100‑1 (MQ=255)
                                                                                             agGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCagagag        >  1:1429596/1‑100 (MQ=255)
                                                                                             agGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCagagag        >  2:936856/1‑100 (MQ=255)
                                                                                              ggTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGt       >  2:449361/1‑100 (MQ=255)
                                                                                               gTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCaagatcgg      >  2:3656733/1‑93 (MQ=255)
                                                                                                   cccTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAg  >  2:495847/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
TTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTAAAG  >  NC_000913/2715934‑2716132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: