Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,724,095 C→A 15.7% intergenic (+13/‑33) pssA → / → yfiM phosphatidylserine synthase; CDP‑diacylglycerol‑serine O‑phosphatidyltransferase/putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,724,0950CA15.7% 582.8 / 87.6 223intergenic (+13/‑33)pssA/yfiMphosphatidylserine synthase; CDP‑diacylglycerol‑serine O‑phosphatidyltransferase/putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (93/94);  new base A (16/19);  total (109/114)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01

Only 100 of 232 total aligned reads displayed.

ATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCT  >  NC_000913/2723996‑2724194
                                                                                                   |                                                                                                   
 ttGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTcc                                                                                                    <  2:1619865/100‑1 (MQ=255)
 ttGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTcc                                                                                                    <  1:3177696/100‑1 (MQ=255)
   gCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTg                                                                                                  >  1:69974/1‑100 (MQ=255)
   gCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTg                                                                                                  >  1:2474030/1‑100 (MQ=255)
     cGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTa                                                                                                >  2:3237315/1‑100 (MQ=255)
      gATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTAc                                                                                               >  2:316322/1‑100 (MQ=255)
        ttATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACgg                                                                                             >  2:941946/1‑100 (MQ=255)
         tATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACggg                                                                                            <  2:2123092/100‑1 (MQ=255)
           tCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCTGCATCCTGTAATCACAACCCCGTCCTGTACGGGgt                                                                                          <  1:4297682/100‑1 (MQ=255)
           tCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgt                                                                                          <  1:4202937/100‑1 (MQ=255)
            ccgatctaGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgt                                                                                          <  2:2541535/92‑1 (MQ=255)
            ccGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgt                                                                                          <  1:818266/99‑1 (MQ=255)
             cGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCGTGTAATCACAACCCCGTTCAGGACGGGgtgg                                                                                        <  2:2979429/100‑3 (MQ=255)
             cGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgttt                                                                                        >  2:4000932/1‑100 (MQ=255)
             cGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgttg                                                                                        <  1:195318/100‑2 (MQ=255)
             cGGTGAAGGTTCGTAAACTCATCCGCCGTGTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgttg                                                                                        <  2:4282277/100‑2 (MQ=255)
              ggTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCTTGTAATCACAACCCCGTACAGGACGGGgttgt                                                                                       <  2:4187940/100‑3 (MQ=255)
               gTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgttgtg                                                                                      <  2:4374846/100‑4 (MQ=255)
               gTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgttgtg                                                                                      <  1:3523544/100‑4 (MQ=255)
                tGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgtttgtt                                                                                     <  2:1820322/100‑1 (MQ=255)
                 aaaGCTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCATCCTGTACGGGggttg                                                                                       >  1:827121/2‑97 (MQ=255)
                   aGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGttttt                                                                                  <  1:4120406/100‑1 (MQ=255)
                   aGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgttgtgatta                                                                                  <  2:2395917/100‑8 (MQ=38)
                    ggTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGtttttt                                                                                 <  1:261713/100‑1 (MQ=255)
                    ggTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGtttttt                                                                                 >  2:2249842/1‑100 (MQ=255)
                      ttCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTgg                                                                               >  2:2041278/1‑100 (MQ=255)
                      ttCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTgg                                                                               <  1:3155510/100‑1 (MQ=255)
                      ttCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTgg                                                                               <  1:257639/100‑1 (MQ=255)
                        cGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAg                                                                             >  2:1169009/1‑100 (MQ=255)
                        cGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAg                                                                             >  2:1598629/1‑100 (MQ=255)
                         gTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAgg                                                                            >  1:3922584/1‑100 (MQ=255)
                          tAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGc                                                                           >  2:1659893/1‑100 (MQ=255)
                              cTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACg                                                                       >  1:2294328/1‑100 (MQ=255)
                                cATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACg                                                                       >  1:4410141/1‑98 (MQ=255)
                                cATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGtt                                                                     >  2:1410122/1‑100 (MQ=255)
                                 aTCCGCCGTTTGCGCCGTATCCACATCGACCGATTAATTAGTCGCATCCTGTAATCACAACCCCGTCCTGTACAGGGTTTGTTTTTTGGAGGCCACGtgt                                                                    <  1:3633443/100‑3 (MQ=255)
                                  tCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGtttt                                                                   <  1:1579598/100‑1 (MQ=255)
                                   ccgccgTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTg                                                                  <  2:4397512/100‑1 (MQ=255)
                                   ccgccgTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTg                                                                  >  1:3800188/1‑100 (MQ=255)
                                     nccgTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCg                                                                >  1:1385764/2‑100 (MQ=255)
                                     gccgTTTGCGCCGTATCCGCCTCGACCGATTCATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCg                                                                <  2:3514113/100‑1 (MQ=255)
                                             cgcCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCtt                                                         >  1:2939020/1‑99 (MQ=255)
                                              gcCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCtttt                                                       <  2:3011279/100‑1 (MQ=255)
                                              gcCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCtttt                                                       <  2:4377921/100‑1 (MQ=255)
                                              gcCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACAGGGTTTGGTTTTTGGAGGCCACGTTTTGCGTAGTCtttt                                                       >  1:1853697/1‑100 (MQ=255)
                                               ccGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCttttt                                                      >  2:476026/1‑100 (MQ=255)
                                                cGTATCCGCAGCGACCCATTAATTAGCCGCAGCCTGTAATCACTACCCCGTCCTGTACTGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTg                                                     <  1:1287209/100‑1 (MQ=255)
                                                  tATCCGCATCGACCGATTAATTAGCCGTATCCTCTAATCACAACCCCGTCCTGTATGGGGTTTGTTTTTTTGAGGCCACGTGTTGCGTATTCTTTTTg                                                     <  1:2520944/98‑1 (MQ=255)
                                                  tATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTc                                                   <  1:488858/100‑1 (MQ=255)
                                                  tATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTc                                                   <  2:1485737/100‑1 (MQ=255)
                                                   aTCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCt                                                  <  2:802283/100‑1 (MQ=255)
                                                   aTCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCt                                                  >  2:2339550/1‑100 (MQ=255)
                                                      cGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGtt                                               >  2:2498684/1‑100 (MQ=255)
                                                      cGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTCTCTGtt                                               >  1:235960/1‑100 (MQ=255)
                                                         aTCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGc                                            <  1:3258601/100‑1 (MQ=255)
                                                            gACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTa                                          <  1:3329995/99‑1 (MQ=255)
                                                            gACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTAt                                         >  2:1239579/1‑100 (MQ=255)
                                                              ccGATTAATTAGCCGCATCCTGTAATCACAACTCCGTCCTTTGCGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTGTTTTTGTTTCTTCGCTagt                                        >  1:1681969/1‑97 (MQ=255)
                                                              ccGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTg                                       <  1:3013721/100‑1 (MQ=255)
                                                                gATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGtt                                     >  2:3772483/1‑100 (MQ=255)
                                                                gATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGtt                                     <  2:3453354/100‑1 (MQ=255)
                                                                 attaattaGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTa                                    >  2:2923208/1‑100 (MQ=255)
                                                                      ttaGCCGCATCCTGTAATCACGACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTc                               <  1:225569/100‑1 (MQ=255)
                                                                       taGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCt                              <  1:4335817/100‑1 (MQ=255)
                                                                        aGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTGTTGTTACTTTCTg                             >  1:1739199/1‑100 (MQ=255)
                                                                         gCCGCATCCTGTGATCACAACCCCGTCCTGTACGGGGGTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTgg                            <  2:1802074/100‑1 (MQ=255)
                                                                         gCCGCATCCTGTAATCACAACCCCGTCCTTTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTgg                            <  2:4090549/100‑1 (MQ=255)
                                                                         gCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTgg                            <  1:3045663/100‑1 (MQ=255)
                                                                          ccGCATCCTGTAATCACAACCCCGTCCTGTACGGGTTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGa                           <  1:4000902/100‑1 (MQ=255)
                                                                          ccGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGa                           <  1:1442788/100‑1 (MQ=255)
                                                                              aTCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCa                       <  2:2811550/100‑1 (MQ=255)
                                                                                 cTGTAATCACAACCCCGTCCTGTACGGGGGTTGTTTTATGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGAAGcc                    >  1:2832193/1‑100 (MQ=255)
                                                                                    aaaaacaaaCCCCGTACAGGACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCata                 >  2:3620100/8‑100 (MQ=38)
                                                                                     aaaacaaaCCCCGTACAGGACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCatat                >  2:2696335/7‑100 (MQ=38)
                                                                                     aaaacaaaCCCCGTACAGGACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTAGCTATTGTTACTTTCTGGATGCAGCCatat                >  2:546559/7‑100 (MQ=38)
                                                                                      aaacaaaCCCCGTACAGGACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATg               >  2:654397/6‑100 (MQ=38)
                                                                                      aTCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATg               <  2:2474042/100‑1 (MQ=255)
                                                                                      aTCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATg               <  2:1919240/100‑1 (MQ=255)
                                                                                        cacaACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGc             >  2:3087676/1‑100 (MQ=255)
                                                                                        cacaACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCGATTGTTACTTTCTGGATGCAGCCATATGGc             <  1:2896226/100‑1 (MQ=255)
                                                                                        acaaaCCCCGTACAGGACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGc             >  2:1482085/4‑100 (MQ=255)
                                                                                         caaaCCCCGTACAGGACGGGGTTTGTTTTTTGTATTCCACGTTTTGCGTATTCTGTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCt            >  1:3741895/3‑100 (MQ=255)
                                                                                         acaACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGTCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCt            >  1:535116/1‑100 (MQ=255)
                                                                                         acaACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTTTGTTCGCTATTGTTACTTTCTGGATGCATCCATATGGCt            >  1:542417/1‑100 (MQ=255)
                                                                                         acaACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCt            <  2:1396240/100‑1 (MQ=255)
                                                                                         acaACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGAAGCCATATGGc             <  1:3688769/99‑1 (MQ=255)
                                                                                          caACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTa           >  1:916916/1‑100 (MQ=255)
                                                                                          caACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTa           <  2:1775504/100‑1 (MQ=255)
                                                                                          caACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTa           >  2:1149606/1‑100 (MQ=255)
                                                                                           aaCCCCGTACAGGACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTaa          >  1:751267/1‑100 (MQ=255)
                                                                                            aCCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAAc         >  2:1994563/1‑100 (MQ=255)
                                                                                            aCCCCGTACAGGACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAAc         >  2:736156/1‑100 (MQ=255)
                                                                                              cccGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGa       >  2:762050/1‑100 (MQ=255)
                                                                                              cccGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTATTATTATCTGTTCGCTATTGTTACTTTCTATATGCAGACATATGGCTAAAGa       >  1:417991/1‑100 (MQ=255)
                                                                                                cGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTTTCTTTTTTTTACTGTTACTTTCTCGATGCAGACATATTGCTAACAATa     >  1:1039816/1‑100 (MQ=255)
                                                                                                cGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATa     <  1:2062381/100‑1 (MQ=255)
                                                                                                 gTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAg    <  2:606922/100‑1 (MQ=255)
                                                                                                  tCCTGTACGGTGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTGCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGc   <  2:3898132/100‑1 (MQ=255)
                                                                                                  tCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGc   <  2:3904345/100‑1 (MQ=255)
                                                                                                   ccTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCt  >  2:2607556/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
ATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCT  >  NC_000913/2723996‑2724194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: