Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,810,872 C→T 15.2% H35Y (CAC→TAC) ‡ mprA → transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,810,8720CT15.2% 342.0 / 44.0 137H35Y (CAC→TAC) mprAtranscriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base C (55/60);  new base T (7/14);  total (62/75)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 162 total aligned reads displayed.

GATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGG  >  NC_000913/2810773‑2810971
                                                                                                   |                                                                                                   
gATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTtgcatgc                                                                                                     <  1:1003849/100‑1 (MQ=255)
 aTAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTtgcatgca                                                                                                    >  2:255228/1‑100 (MQ=255)
   aGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcaca                                                                                                  >  1:3255227/1‑100 (MQ=255)
    gTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcacat                                                                                                 >  1:710407/1‑100 (MQ=255)
      tCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcacatgc                                                                                               <  2:57792/100‑1 (MQ=255)
      tCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcacatgc                                                                                               <  2:3362330/100‑1 (MQ=255)
       cGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgtgcatgca                                                                                              <  2:3761636/100‑1 (MQ=255)
        gTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCaa                                                                                             >  1:2925248/1‑100 (MQ=255)
        gTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCaa                                                                                             >  1:2285319/1‑100 (MQ=255)
         tttACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCaaa                                                                                            <  2:3321268/100‑1 (MQ=255)
          ttACTCCCATTAAACAAATGCTAAAATTTCGCGCCAACTGCCACGAAGATTTTCCTTATCAGGAGATACTTCTTACTCGTCTTTGCATGCACATACaaa                                                                                            >  1:467376/1‑99 (MQ=255)
          ttACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaag                                                                                           <  1:4076645/100‑1 (MQ=255)
          tcttccgatctGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaag                                                                                           <  1:3278967/90‑1 (MQ=255)
           tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaaga                                                                                          <  2:2740667/100‑2 (MQ=255)
           tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaaga                                                                                          <  1:3842242/100‑2 (MQ=255)
           tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaaga                                                                                          <  1:1496042/100‑2 (MQ=255)
           tACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaag                                                                                           <  1:2780490/99‑1 (MQ=255)
            aCGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagac                                                                                         <  2:3352853/100‑3 (MQ=255)
            aCGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagca                                                                                         <  2:1507365/100‑1 (MQ=255)
            aCGCCCATGTACCAAATGCTAACATTTCGCGCCAGCCGCCACTAAGATTTTCCTTATCAGGATATCCTTCTGACTCGTCTTTGCATGCACATGCaaat                                                                                           <  1:4210457/98‑2 (MQ=255)
             cGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagacg                                                                                        <  2:3577209/100‑4 (MQ=255)
             cGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagcaa                                                                                        >  2:143748/1‑100 (MQ=255)
             cGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagcaa                                                                                        >  2:840286/1‑100 (MQ=255)
             cGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagcaa                                                                                        >  2:1310928/1‑100 (MQ=255)
             cGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagcaa                                                                                        >  1:1526533/1‑100 (MQ=255)
             cGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGGCTTTGCATGTGCATGCAaagacg                                                                                        >  1:2187004/1‑97 (MQ=255)
               cccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGTGCATGCAaagacgag                                                                                      <  1:3222950/100‑6 (MQ=255)
               cccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagcaagc                                                                                      <  2:25155/100‑1 (MQ=255)
               cccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGGCTTTGCATGTGCATGCAaagacgag                                                                                      <  2:2187011/100‑6 (MQ=38)
                ccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAgct                                                                                     <  2:3270719/100‑1 (MQ=255)
                ccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAgct                                                                                     >  1:1159243/1‑100 (MQ=255)
                 cATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAgctg                                                                                    >  1:468618/1‑100 (MQ=255)
                    tGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTGTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAcctgc                                                                                   >  1:3866455/1‑98 (MQ=255)
                     gAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTgg                                                                                <  1:2856455/100‑1 (MQ=255)
                     gAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTgg                                                                                <  1:2794467/100‑1 (MQ=255)
                     gAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTgg                                                                                <  1:3241383/100‑1 (MQ=255)
                     gAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTgg                                                                                >  1:2128077/1‑100 (MQ=255)
                     gAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTgg                                                                                <  1:3132005/100‑1 (MQ=255)
                     gAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTgg                                                                                >  1:541902/1‑100 (MQ=255)
                      aaCAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGga                                                                               >  1:848943/1‑100 (MQ=255)
                          aaTGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAAc                                                                           >  1:2469495/1‑100 (MQ=255)
                            tGCTAAGATTTCGCACCCGCCGCCACGAAGATTTTCCTATCAGGAGAACCCTCTGACTCGCCTTTGCATGCACACGCAAAGCAAGCTGCTGGAGAACCGc                                                                        <  1:866839/100‑1 (MQ=255)
                             gCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGc                                                                        <  2:568064/100‑1 (MQ=255)
                              cTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCATGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCa                                                                       >  1:297578/1‑100 (MQ=255)
                              cTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCa                                                                       >  2:4266128/1‑100 (MQ=255)
                              cTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCa                                                                       >  2:2085323/1‑100 (MQ=255)
                               tAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCaa                                                                      >  2:2902076/1‑100 (MQ=255)
                                    tttCGCGCCAGCCGCGACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATaaaa                                                                 <  2:151944/100‑1 (MQ=255)
                                     ttCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGAACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAAt                                                                <  1:1291930/100‑1 (MQ=255)
                                      tCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAAt                                                                <  2:3643421/99‑1 (MQ=255)
                                      tCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCGGGAGAACCGCAATaaaa                                                                 <  1:3847416/98‑1 (MQ=255)
                                        gcgcCAGCCGCCACGAAGATTTTCCTGATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCt                                                             >  2:2693469/1‑100 (MQ=255)
                                          gcCAGCCGCCACGAATATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGa                                                           <  2:4349634/100‑1 (MQ=255)
                                           ccAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGaa                                                          >  2:1816119/1‑100 (MQ=255)
                                            cAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAg                                                         <  1:739572/100‑1 (MQ=255)
                                            cAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAg                                                         <  1:1863187/100‑1 (MQ=255)
                                             aGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAgg                                                        <  1:76994/100‑1 (MQ=255)
                                                   cACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAgg                                                  >  2:3828846/1‑100 (MQ=255)
                                                   cACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAgg                                                  <  2:516502/100‑1 (MQ=255)
                                                     cGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAgggg                                                >  1:2048815/1‑100 (MQ=255)
                                                     cGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAgggg                                                >  2:3239339/1‑100 (MQ=255)
                                                     cGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAgggg                                                >  1:3951528/1‑100 (MQ=255)
                                                      gAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGa                                               >  2:2615376/1‑100 (MQ=255)
                                                            tttCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACg                                         <  1:4350260/100‑1 (MQ=255)
                                                               ccTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACgaga                                      <  2:2128084/100‑1 (MQ=255)
                                                                cTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGAc                                     >  1:3187458/1‑100 (MQ=255)
                                                                  tATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgt                                   >  2:809087/1‑100 (MQ=255)
                                                                    tCAGGAGATCCTTCTGACTCGTCTTTGCAGGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttg                                 <  1:1830006/100‑1 (MQ=255)
                                                                      aGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCTAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt                               >  1:3919226/1‑100 (MQ=255)
                                                                      aGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGCTTGACGGGACg                                    >  2:2569710/1‑95 (MQ=255)
                                                                      aGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt                               <  2:1933087/100‑1 (MQ=255)
                                                                      aGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt                               >  2:1886732/1‑100 (MQ=255)
                                                                      aGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt                               <  2:1124659/100‑1 (MQ=255)
                                                                       ggAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGttt                              >  1:3636655/1‑100 (MQ=255)
                                                                       ggAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGttt                              >  1:1592265/1‑100 (MQ=255)
                                                                        gagaTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTa                             <  2:731847/100‑1 (MQ=255)
                                                                         agaTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTAt                            <  2:3251519/100‑1 (MQ=255)
                                                                          gaTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTAt                            >  2:921690/1‑99 (MQ=255)
                                                                          gaTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATg                           >  2:3087084/1‑100 (MQ=255)
                                                                           aaccTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATgg                          <  1:2669878/98‑1 (MQ=255)
                                                                           aTCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATgg                          <  2:2422499/100‑1 (MQ=255)
                                                                               ttCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGtt                      <  2:3155426/100‑1 (MQ=255)
                                                                                tCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTg                     <  2:1012659/100‑1 (MQ=255)
                                                                                tCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTg                     <  1:3546117/100‑1 (MQ=255)
                                                                                tCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTg                     <  2:1088081/100‑1 (MQ=255)
                                                                                  tGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAt                   <  2:2301648/100‑1 (MQ=255)
                                                                                  tGACTCGTCTTGGCATGCACATGCAAAGCGAGCTGCTTGATAACCGTAATAAAATGCTGAAGGCTCAGGGGACTAATGAGACGTTGTTTATGGCGTTg                     <  1:2167921/98‑1 (MQ=255)
                                                                                     gcttgcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTAc                >  2:1596331/6‑100 (MQ=255)
                                                                                     gcttgcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTAc                >  2:1660694/6‑100 (MQ=255)
                                                                                     gcttgcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTAc                >  2:2489506/6‑100 (MQ=255)
                                                                                     cTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTAc                >  2:1326063/1‑100 (MQ=255)
                                                                                      cttgcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACg               >  2:634419/5‑100 (MQ=255)
                                                                                        tgcTTTGCATGTGCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCt             >  2:4119463/3‑100 (MQ=255)
                                                                                           tttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGa          >  1:3032469/1‑100 (MQ=255)
                                                                                            ttGCATGCACATGCAAAGCAAGCTGCTGGAGAGTCGCAATAAAATGCTGAAGGCGCAGGGGAGTAACGAGACGTTGTTTATGGCGTTGATTTCGCTGGa          <  1:2320038/99‑1 (MQ=255)
                                                                                            ttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAg         <  1:3562380/100‑1 (MQ=255)
                                                                                            ttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAg         <  1:1305120/100‑1 (MQ=255)
                                                                                             tgcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGt        >  1:3146274/1‑100 (MQ=255)
                                                                                                  gcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAg   <  2:1831454/100‑1 (MQ=255)
                                                                                                   cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAgg  <  2:643674/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGG  >  NC_000913/2810773‑2810971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: