Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,156,590 C→A 15.9% intergenic (+72/‑33) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,156,5900CA15.9% 476.4 / 71.8 178intergenic (+72/‑33)yqhD/dkgAaldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
Reads supporting (aligned to +/- strand):  ref base C (74/74);  new base A (15/13);  total (89/89)
Fisher's exact test for biased strand distribution p-value = 8.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

Only 100 of 184 total aligned reads displayed.

CGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCT  >  NC_000913/3156492‑3156689
                                                                                                  |                                                                                                   
 gccgTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCt                                                                                                    >  1:4126558/1‑99 (MQ=255)
      atataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCg                                                                                              >  2:3277287/1‑100 (MQ=255)
      atataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCg                                                                                              >  2:1493615/1‑100 (MQ=255)
      atataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCg                                                                                              >  2:2563740/1‑100 (MQ=255)
       tataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCGCCGGAGCCTGCTCCgg                                                                                             >  2:1468254/1‑100 (MQ=255)
        ataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGt                                                                                            <  1:1447042/100‑1 (MQ=255)
        ataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTGTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGt                                                                                            <  1:1838135/100‑1 (MQ=255)
         taCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTg                                                                                           >  2:1305957/1‑100 (MQ=255)
          aCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGa                                                                                          <  2:2670411/100‑1 (MQ=255)
           cGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAg                                                                                         <  2:1875014/100‑1 (MQ=255)
           cGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGAg                                                                                         <  1:55432/100‑1 (MQ=255)
           cGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGAg                                                                                         <  2:620766/100‑1 (MQ=255)
            gAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGt                                                                                        <  2:773082/100‑1 (MQ=255)
            gAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGt                                                                                        <  2:2510796/100‑1 (MQ=255)
            gAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGt                                                                                        >  1:1152087/1‑100 (MQ=255)
             aaGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGtt                                                                                       <  1:1565258/100‑1 (MQ=255)
             aaGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGAggt                                                                                       <  2:4054274/100‑3 (MQ=255)
               gccgccCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCCTTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGATTTCa                                                                                     <  1:1858478/100‑1 (MQ=255)
               gccgccCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCa                                                                                     >  2:2917151/1‑100 (MQ=255)
                ccgcccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCat                                                                                    >  2:1141799/1‑100 (MQ=255)
                 cgcccgcTAAGCTTTTTCCGCCTCAAACTTTCGTTTGCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGAggt                                                                                       <  1:4169260/96‑3 (MQ=38)
                 cgcccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCata                                                                                   <  1:1498870/100‑1 (MQ=255)
                 cgcccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCata                                                                                   <  2:66343/100‑1 (MQ=255)
                   cccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCTCCGGAGCCTGCTCCGGTGAGTTCAtata                                                                                 <  2:1521038/100‑1 (MQ=255)
                   cccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCAtata                                                                                 >  1:2494735/1‑100 (MQ=255)
                     cgcTAAGCTTTTTACGCCTCAAACTTTCCTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCTTAGCCTTCTCCGGTGAGATCacat                                                                                  <  1:1058862/97‑1 (MQ=255)
                       cTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAagg                                                                             >  1:377698/1‑100 (MQ=255)
                       cTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAagg                                                                             <  2:3141573/100‑1 (MQ=255)
                        tAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAagga                                                                            >  2:3851041/1‑100 (MQ=255)
                        tAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAagga                                                                            >  2:3168042/1‑100 (MQ=255)
                         aaGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAaggag                                                                           <  1:259694/100‑1 (MQ=255)
                          aGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAaggagg                                                                          >  1:3782173/1‑100 (MQ=255)
                          aGCTTTTTACGCCTCAAACTTTCGGTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTACGGTGAGTTCATATAAaggagg                                                                          >  1:853525/1‑100 (MQ=255)
                           gCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAggagga                                                                         >  1:375315/1‑100 (MQ=255)
                           gCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAggagga                                                                         >  2:3421085/1‑100 (MQ=255)
                             tttttACGCCTCAAAATTTCGTTTTCGGGCATTTCGTCCAGACTGAAGTTCACAACACCTCACCGGAGCCTGGTCCGGTGAGTTCATATAAAGGAGGAAc                                                                       >  1:649930/1‑100 (MQ=255)
                                  aCGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATg                                                                  <  1:1341473/100‑1 (MQ=255)
                                  aCGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATg                                                                  <  2:4164745/100‑1 (MQ=255)
                                   cGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATgg                                                                 >  2:1924799/1‑100 (MQ=255)
                                    gCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGc                                                                <  1:3424771/100‑1 (MQ=255)
                                    gCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGc                                                                >  1:3642745/1‑100 (MQ=255)
                                     ccTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCt                                                               >  2:3021988/1‑100 (MQ=255)
                                     ccTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCt                                                               >  1:1354369/1‑100 (MQ=255)
                                            cTTTCGTTTTCAGGTATTTCGTCCAGACTTAAGTTCACAACACAGCGCCGGAGCCTGCTCCGGTGAGTTCATATAAAGGGGGAACGTATGGCTAATcc                                                          <  1:1991564/98‑1 (MQ=255)
                                            cTTTCGTGTTCGGGCATTTCGTCCCGACTTAAGATCACAACACCTCCCCTGCGCCTGCGCCGGTGATGTCATATGAAGGAGGCACGGATGGCTAATACa                                                         >  1:3488798/1‑99 (MQ=255)
                                               tCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCg                                                     <  1:4323845/100‑1 (MQ=255)
                                               tCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCg                                                     <  2:2696748/100‑1 (MQ=255)
                                               tCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCg                                                     <  2:2375/100‑1 (MQ=255)
                                               tCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCg                                                     <  2:2123959/100‑1 (MQ=255)
                                                cGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGt                                                    >  2:1183559/1‑100 (MQ=255)
                                                 gTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGTGCCTGCTCCGTTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGtt                                                   >  1:2440295/1‑100 (MQ=255)
                                                 gTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGtt                                                   >  1:804121/1‑100 (MQ=255)
                                                 gTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGtt                                                   >  1:953561/1‑100 (MQ=255)
                                                 gTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGtt                                                   >  2:1781781/1‑100 (MQ=255)
                                                    ttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttatt                                                >  1:632819/1‑100 (MQ=255)
                                                     tCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttatta                                               >  2:4155027/1‑100 (MQ=255)
                                                       gggCATTTCGTCCAGACTTAAGTTCACAACACCACACCGGAGCGTGTTCCGGTGAGTTCATATAAAAGAGGGACGTATGGTTAATCCAACCGTTATTaa                                              <  1:2792530/99‑1 (MQ=255)
                                                        ggCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGc                                            <  2:1256143/100‑1 (MQ=255)
                                                         gCATTTCGCCCAGACTGAAGTTAACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttatt                                                <  1:1616548/95‑1 (MQ=255)
                                                           aTTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTAc                                         >  1:1118441/1‑100 (MQ=255)
                                                            tttCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACa                                        <  2:3663029/100‑1 (MQ=255)
                                                            tttCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACa                                        <  2:1118601/100‑1 (MQ=255)
                                                                gTCCAGACTTAAGTTAACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGATAATCCAAACGTTATTAAGCTACAGGa                                     >  1:2298319/1‑99 (MQ=255)
                                                                 tCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATg                                   >  2:568538/1‑100 (MQ=255)
                                                                 tCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAAAGTATGGCTAATCCAACCGTTATTAAGATACAgg                                      >  1:3979572/1‑97 (MQ=255)
                                                                  ccAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATgg                                  >  2:1107600/1‑100 (MQ=255)
                                                                  ccAGACTTAAGTTCACAACACCCTACCGGAGCCTTCTCCGGTAAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATg                                   <  1:1005600/99‑1 (MQ=255)
                                                                    aGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCa                                >  1:1738106/1‑100 (MQ=255)
                                                                       cTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATg                             >  2:1627737/1‑100 (MQ=255)
                                                                       cTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATg                             <  2:408167/100‑1 (MQ=255)
                                                                        ttAAGTTCACAACACCTCACCGGAGCCGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGt                            <  1:3038171/100‑1 (MQ=255)
                                                                          aaGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCa                          <  1:253717/100‑1 (MQ=255)
                                                                            gTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCAt                         >  1:3417347/1‑99 (MQ=255)
                                                                            gTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATg                        >  2:3747838/1‑100 (MQ=255)
                                                                              tCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGcc                      <  1:4037549/100‑1 (MQ=255)
                                                                               cacaACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGccc                     >  2:2866927/1‑100 (MQ=255)
                                                                                 caacaCCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCa                   >  2:3977391/1‑100 (MQ=255)
                                                                                   tgaacTCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAACCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGc                 >  1:3306589/5‑100 (MQ=38)
                                                                                   acacCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGc                 >  2:3548887/1‑100 (MQ=255)
                                                                                     acCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTg               <  1:4176197/100‑1 (MQ=255)
                                                                                      ccTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCACCCCTTATTACGCTACAGGATGGCAATGTCATGCCCCAGCTgg              >  1:3096891/1‑100 (MQ=255)
                                                                                      ccTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTgg              <  2:4222506/100‑1 (MQ=255)
                                                                                       cTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTggg             <  2:827732/100‑1 (MQ=255)
                                                                                       cTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTggg             >  2:1603305/1‑100 (MQ=255)
                                                                                       cTCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTggg             >  2:3254348/1‑100 (MQ=255)
                                                                                       cTCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTggg             >  2:1768677/1‑100 (MQ=255)
                                                                                       cTCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTggg             >  2:4002718/1‑100 (MQ=255)
                                                                                        tCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGa            >  2:2367331/1‑100 (MQ=255)
                                                                                         cACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGAc           <  2:1866302/100‑1 (MQ=255)
                                                                                         cACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTggg             >  1:1457925/1‑98 (MQ=255)
                                                                                         cACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGAc           >  2:1186250/1‑100 (MQ=255)
                                                                                         cACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGAc           >  2:2230761/1‑100 (MQ=255)
                                                                                             ggAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTggg       <  1:4339115/100‑1 (MQ=255)
                                                                                             ggAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTggg       <  1:3550156/100‑1 (MQ=255)
                                                                                             ggAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTggg       <  1:3215481/100‑1 (MQ=255)
                                                                                             ggAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATACCCCAGCTGGGACTggg       >  1:46665/1‑100 (MQ=255)
                                                                                               aGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCg     <  2:3284255/100‑1 (MQ=255)
                                                                                                gCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGt    >  2:2285575/1‑100 (MQ=255)
                                                                                                gCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGt    >  1:2527178/1‑100 (MQ=255)
                                                                                                  cTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCt  >  2:3083810/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
CGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCT  >  NC_000913/3156492‑3156689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: