Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,156,591 T→G 15.8% intergenic (+73/‑32) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,156,5910TG15.8% 452.7 / 80.8 182intergenic (+73/‑32)yqhD/dkgAaldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
Reads supporting (aligned to +/- strand):  ref base T (78/75);  new base G (15/14);  total (93/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.32e-01

Only 100 of 188 total aligned reads displayed.

CGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTG  >  NC_000913/3156492‑3156690
                                                                                                   |                                                                                                   
 gccgTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCt                                                                                                     >  1:4126558/1‑99 (MQ=255)
      atataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCg                                                                                               >  2:3277287/1‑100 (MQ=255)
        ataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGt                                                                                             <  2:180362/100‑1 (MQ=255)
         taCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTg                                                                                            >  1:3923143/1‑100 (MQ=255)
         taCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTg                                                                                            <  2:134427/100‑1 (MQ=255)
          aCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGa                                                                                           <  2:2672171/100‑1 (MQ=255)
           cGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAg                                                                                          <  2:1875014/100‑1 (MQ=255)
           cGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGAg                                                                                          <  1:55432/100‑1 (MQ=255)
           cGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGAg                                                                                          <  2:620766/100‑1 (MQ=255)
            gAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGt                                                                                         >  1:1152087/1‑100 (MQ=255)
            gAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGt                                                                                         <  2:773082/100‑1 (MQ=255)
              aGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGAggtg                                                                                       <  1:4386431/100‑4 (MQ=39)
               gccgccCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCCTTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGATTTCa                                                                                      <  1:1858478/100‑1 (MQ=255)
                ccgcccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCat                                                                                     <  2:2601239/100‑1 (MQ=255)
                 cgcccgcTAAGCTTTTTCCGCCTCAAACTTTCGTTTGCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGAggt                                                                                        <  1:4169260/96‑3 (MQ=38)
                 cgcccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCata                                                                                    <  1:1498870/100‑1 (MQ=255)
                 cgcccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCata                                                                                    <  1:1692930/100‑1 (MQ=255)
                 cgcccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCata                                                                                    <  2:66343/100‑1 (MQ=255)
                  gcccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACAACTCACCGGAGCAGGCTCCGGTGAggtgttgt                                                                                   <  1:4334526/100‑8 (MQ=37)
                   cccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCTCCGGAGCCTGCTCCGGTGAGTTCAtata                                                                                  <  2:1521038/100‑1 (MQ=255)
                   cccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCAtata                                                                                  <  1:1254243/100‑1 (MQ=255)
                     cgcTAAGCTTTTTACGCCTCAAACTTTCCTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCTTAGCCTTCTCCGGTGAGATCacat                                                                                   <  1:1058862/97‑1 (MQ=255)
                       cTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAagg                                                                              <  2:3141573/100‑1 (MQ=255)
                        tAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAagga                                                                             >  2:3851041/1‑100 (MQ=255)
                        tAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAagga                                                                             >  2:3168042/1‑100 (MQ=255)
                         aaGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAaggag                                                                            <  1:259694/100‑1 (MQ=255)
                           gCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAggagga                                                                          >  1:375315/1‑100 (MQ=255)
                            cTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAggagga                                                                          <  1:4180656/99‑1 (MQ=255)
                             tttttACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAAc                                                                        <  2:2720060/100‑1 (MQ=255)
                                  aCGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATg                                                                   <  2:4164745/100‑1 (MQ=255)
                                   cGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATgg                                                                  >  2:1924799/1‑100 (MQ=255)
                                    gCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGc                                                                 <  1:3424771/100‑1 (MQ=255)
                                    gCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGc                                                                 >  1:3642745/1‑100 (MQ=255)
                                     ccTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCt                                                                >  2:3021988/1‑100 (MQ=255)
                                     ccTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGCTCACAACCCCTCACCGGAGCCTGCTCCGGTAAGTTCATATAAAGGAGGAACGTATGGc                                                                 >  1:861928/1‑99 (MQ=255)
                                         aaaCTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATc                                                            <  2:1269034/100‑1 (MQ=255)
                                            cTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCaa                                                         >  2:1671400/1‑100 (MQ=255)
                                            cTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGATCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCaa                                                         >  2:118617/1‑100 (MQ=255)
                                            cTTTCGTTTTCAGGTATTTCGTCCAGACTTAAGTTCACAACACAGCGCCGGAGCCTGCTCCGGTGAGTTCATATAAAGGGGGAACGTATGGCTAATcc                                                           <  1:1991564/98‑1 (MQ=255)
                                            cTTTCGTGTTCGGGCATTTCGTCCCGACTTAAGATCACAACACCTCCCCTGCGCCTGCGCCGGTGATGTCATATGAAGGAGGCACGGATGGCTAATACa                                                          >  1:3488798/1‑99 (MQ=255)
                                             tttCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGGACGTATGGCTAATCCAAc                                                        >  2:4003050/1‑100 (MQ=255)
                                             tttCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAAc                                                        <  1:2499805/100‑1 (MQ=255)
                                              aaagtttgagggCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAAcc                                                       <  1:794598/91‑1 (MQ=255)
                                               tCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCg                                                      <  2:2123959/100‑1 (MQ=255)
                                                cGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGt                                                     >  2:1183559/1‑100 (MQ=255)
                                                 gTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGTGCCTGCTCCGTTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGtt                                                    >  1:2440295/1‑100 (MQ=255)
                                                  ttttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGtta                                                   >  2:2382051/1‑100 (MQ=255)
                                                    ttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttatt                                                 >  1:632819/1‑100 (MQ=255)
                                                     tCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttatta                                                >  2:4155027/1‑100 (MQ=255)
                                                       gggCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAg                                              >  1:1523056/1‑100 (MQ=255)
                                                       gggCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAg                                              <  1:1604047/100‑1 (MQ=255)
                                                       gggCATTTCGTCCAGACTTAAGTTCACAACACCACACCGGAGCGTGTTCCGGTGAGTTCATATAAAAGAGGGACGTATGGTTAATCCAACCGTTATTaa                                               <  1:2792530/99‑1 (MQ=255)
                                                        ggCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGc                                             <  2:1256117/100‑1 (MQ=255)
                                                         gCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCt                                            <  2:2540051/100‑1 (MQ=255)
                                                         gCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCt                                            <  1:516253/100‑1 (MQ=255)
                                                           aTTTCGTCCAGACTTAAGTTCACAACACCTCACCGGGGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTAc                                          >  2:3101269/1‑100 (MQ=255)
                                                           aTTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTAc                                          >  1:1118441/1‑100 (MQ=255)
                                                            tttCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACa                                         <  2:3663029/100‑1 (MQ=255)
                                                                gTCCAGACTTAAGTTAACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGATAATCCAAACGTTATTAAGCTACAGGa                                      >  1:2298319/1‑99 (MQ=255)
                                                                 tCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATg                                    >  2:568538/1‑100 (MQ=255)
                                                                 tCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAAAGTATGGCTAATCCAACCGTTATTAAGATACAgg                                       >  1:3979572/1‑97 (MQ=255)
                                                                  ccAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATgg                                   <  2:1118444/100‑1 (MQ=255)
                                                                  ccAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATgg                                   <  2:3492773/100‑1 (MQ=255)
                                                                  ccAGACTTAAGTTCACAACACCCTACCGGAGCCTTCTCCGGTAAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATg                                    <  1:1005600/99‑1 (MQ=255)
                                                                       cTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATg                              >  2:1627737/1‑100 (MQ=255)
                                                                        ttAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGt                             <  1:1350452/100‑1 (MQ=255)
                                                                        ttAAGTTCACAACACCTCACCGGAGCCGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGt                             <  1:3038171/100‑1 (MQ=255)
                                                                         tAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGCGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTc                            >  1:988554/1‑100 (MQ=255)
                                                                          aaGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCa                           <  1:253717/100‑1 (MQ=255)
                                                                            gTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCAt                          >  1:3417347/1‑99 (MQ=255)
                                                                            gTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATg                         >  2:3747838/1‑100 (MQ=255)
                                                                              tCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGcc                       <  1:4037549/100‑1 (MQ=255)
                                                                              tCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGcc                       <  2:3979602/100‑1 (MQ=255)
                                                                               cacaACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGccc                      >  2:1777237/1‑100 (MQ=255)
                                                                               cacaACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGccc                      >  1:1791316/1‑100 (MQ=255)
                                                                                   tgaacTCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAACCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGc                  >  1:3306589/5‑100 (MQ=38)
                                                                                   acacCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGc                  >  2:3548887/1‑100 (MQ=255)
                                                                                     acCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTg                >  2:2876783/1‑100 (MQ=255)
                                                                                     aacTCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTg                >  2:1126020/3‑100 (MQ=255)
                                                                                      ccTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCACCCCTTATTACGCTACAGGATGGCAATGTCATGCCCCAGCTgg               >  1:3096891/1‑100 (MQ=255)
                                                                                      ccTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTgg               <  2:4222506/100‑1 (MQ=255)
                                                                                       cgcACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTggg              >  2:4090244/3‑100 (MQ=255)
                                                                                       cTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTggg              <  2:827732/100‑1 (MQ=255)
                                                                                       cTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTggg              >  2:1603305/1‑100 (MQ=255)
                                                                                       cTCACCGGAGCCTGCGCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATTTCATGCCCCAGCTggn              <  1:804509/100‑2 (MQ=255)
                                                                                       cTCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTggg              >  2:3254348/1‑100 (MQ=255)
                                                                                       cTCACCGGAGCAGGCGCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTggg              >  2:3029454/1‑100 (MQ=255)
                                                                                        tCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGa             >  2:2367331/1‑100 (MQ=255)
                                                                                         cACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGAc            >  2:3379050/1‑100 (MQ=255)
                                                                                         cACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTggg              >  1:1457925/1‑98 (MQ=255)
                                                                                         cACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGAc            >  2:1469683/1‑100 (MQ=255)
                                                                                         cACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGAc            >  2:2230761/1‑100 (MQ=255)
                                                                                         cACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGAc            >  2:4059614/1‑100 (MQ=255)
                                                                                         cACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGAGAc            >  1:1406343/1‑100 (MQ=255)
                                                                                             ggAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTggg        <  1:3215481/100‑1 (MQ=255)
                                                                                             ggAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTggg        <  1:3550156/100‑1 (MQ=255)
                                                                                             ggAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTggg        <  1:2457339/100‑1 (MQ=255)
                                                                                                gCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGt     >  2:2285575/1‑100 (MQ=255)
                                                                                                 ccTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTc    >  1:3781604/1‑100 (MQ=255)
                                                                                                   tGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTg  <  2:2269440/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTG  >  NC_000913/3156492‑3156690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: