Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,171,852 C→T 15.1% intergenic (+19/+27) qseC → / ← ygiZ quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,171,8520CT15.1% 518.7 / 67.1 211intergenic (+19/+27)qseC/ygiZquorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (84/94);  new base T (18/14);  total (103/108)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

Only 100 of 216 total aligned reads displayed.

GAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACA  >  NC_000913/3171754‑3171951
                                                                                                  |                                                                                                   
gAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGcc                                                                                                    <  2:1793855/100‑1 (MQ=255)
 aaTCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCg                                                                                                   <  1:2230996/100‑1 (MQ=255)
   tCGCCAAATTGCATGGCATGAATGTTGACTTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGtt                                                                                                 >  1:1993147/1‑100 (MQ=255)
   tCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGtt                                                                                                 >  1:575611/1‑100 (MQ=255)
    cGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTa                                                                                                >  1:3215916/1‑100 (MQ=255)
     gCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaa                                                                                               >  2:807477/1‑100 (MQ=255)
     gCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaa                                                                                               >  2:3489957/1‑100 (MQ=255)
     gCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaa                                                                                               >  1:1202173/1‑100 (MQ=255)
      ccAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaa                                                                                              <  2:4024852/100‑1 (MQ=255)
       cAAATTGCATGGGAGGAATGGTGAATTGTGCAATGCGTAAAAAGGTCGATTTGAGGCTAAGGTAAGCTGTTAACTTCTCTGTCTATGAGAGCCGtt                                                                                                 <  1:4268058/96‑1 (MQ=255)
       cAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaaa                                                                                             >  2:4077366/1‑100 (MQ=255)
       cAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaaa                                                                                             >  2:2823814/1‑100 (MQ=255)
        aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAc                                                                                            >  2:2755769/1‑100 (MQ=255)
        aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAc                                                                                            >  2:3919946/1‑100 (MQ=255)
           ttGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGttttaacggc                                                                                         >  2:534320/1‑92 (MQ=255)
           ttGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGAc                                                                                         <  1:4356751/100‑1 (MQ=255)
              catggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGActct                                                                                      <  2:113472/100‑1 (MQ=255)
              catggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGActct                                                                                      >  2:3382352/1‑100 (MQ=255)
               atggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACtctc                                                                                     <  2:1467791/100‑1 (MQ=255)
               atggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACtctc                                                                                     >  1:1719929/1‑100 (MQ=255)
                 ggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCAt                                                                                   <  2:1565139/100‑1 (MQ=38)
                 ggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCAt                                                                                   <  2:2302219/100‑1 (MQ=38)
                 ggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCAt                                                                                   >  2:3629112/1‑100 (MQ=255)
                 ggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCAt                                                                                   <  2:2485837/100‑1 (MQ=255)
                  gcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATa                                                                                  <  2:231597/100‑1 (MQ=38)
                  gcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATa                                                                                  >  1:1863309/1‑100 (MQ=255)
                     tgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGac                                                                               <  2:2034586/100‑2 (MQ=38)
                     tgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGAt                                                                               >  2:1291362/1‑100 (MQ=255)
                      gAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGaca                                                                              <  1:1729972/100‑3 (MQ=37)
                         tGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTa                                                                           >  2:3235317/1‑100 (MQ=255)
                          gTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTAc                                                                          >  2:3315369/1‑100 (MQ=255)
                          gTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTAc                                                                          <  1:98903/100‑1 (MQ=255)
                           ttGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGacagagaa                                                                         <  2:2838521/100‑8 (MQ=25)
                            tGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCGGTTAAAACGAGTCTCATAGATTGTACTa                                                                        >  1:1989946/1‑100 (MQ=255)
                            tGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTa                                                                        >  1:3924515/1‑100 (MQ=255)
                            tGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTa                                                                        >  2:3683695/1‑100 (MQ=255)
                             gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACtca                                                                       >  2:3291839/1‑98 (MQ=255)
                              cgatctggAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAAt                                                                      <  1:804313/94‑1 (MQ=255)
                              aaTTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTTTGTCTATTAGAGCCGTTAAAACGACTCTCATAGATTTTACt                                                                         <  1:679434/97‑1 (MQ=255)
                               aTTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCCTTAAAACGACTCTCATAGATTTTACTAATa                                                                     <  1:2895978/100‑1 (MQ=255)
                                tttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAg                                                                    <  2:797968/100‑1 (MQ=255)
                                tttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAg                                                                    <  2:453030/100‑1 (MQ=255)
                                 ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                   >  1:1342217/1‑100 (MQ=255)
                                    ggAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGGGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaaa                                                                >  2:1272955/1‑100 (MQ=255)
                                    ggAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaaa                                                                >  2:1057691/1‑100 (MQ=255)
                                    ggAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaaa                                                                <  1:3571866/100‑1 (MQ=255)
                                     gAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaaaa                                                               <  1:1291357/100‑1 (MQ=255)
                                      aaTGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaaaa                                                               <  2:1599430/99‑1 (MQ=255)
                                      aaTGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAat                                                              <  1:1126043/100‑1 (MQ=255)
                                      aaTGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAat                                                              <  1:1506724/100‑1 (MQ=255)
                                       aTGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAata                                                             >  2:2265767/1‑100 (MQ=255)
                                           ggAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATaaa                                                         >  1:4083649/1‑100 (MQ=255)
                                             aaCAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAAcc                                                       <  1:3703220/100‑1 (MQ=255)
                                               cAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGt                                                     >  1:35093/1‑100 (MQ=255)
                                                  ggTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGGccc                                                  <  1:1212268/100‑1 (MQ=255)
                                                   gTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTcccc                                                 >  1:4207155/1‑100 (MQ=255)
                                                      gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaa                                              >  2:1166784/1‑100 (MQ=255)
                                                        tttGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaa                                            >  2:2879144/1‑100 (MQ=255)
                                                        tttGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCATACCCaaaaa                                            <  1:236701/100‑1 (MQ=255)
                                                         ttGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaaa                                           <  1:3260445/100‑1 (MQ=255)
                                                          tGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAg                                          <  2:2149564/100‑1 (MQ=255)
                                                          tGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAg                                          <  2:4219771/100‑1 (MQ=255)
                                                          tGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAg                                          <  2:4293586/100‑1 (MQ=255)
                                                             ggCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCACAATATAAACCGTCCCCAAAAAAGcca                                       >  1:439804/1‑100 (MQ=255)
                                                             ggCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcca                                       >  2:207919/1‑100 (MQ=255)
                                                               cGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccacc                                     <  2:2920962/100‑1 (MQ=255)
                                                               cGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccac                                      >  2:3129429/1‑99 (MQ=255)
                                                                  aGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGACCCCAAAAAAGCCaccaac                                  <  1:3114034/100‑1 (MQ=255)
                                                                      aaGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcaca                              <  2:19926/100‑1 (MQ=255)
                                                                          tGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAcc                          <  1:2801346/100‑1 (MQ=255)
                                                                          tGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAcc                          <  2:2671097/100‑1 (MQ=255)
                                                                           ggTAACTTCTCTGTATATGAGAGCCGTTAATAAGACTCTCATAGATTTTACTCATACCAAAATATAACCCGTCCCCACAAAACCCATCATCCACAAAcaa                         >  1:1407104/1‑98 (MQ=255)
                                                                              attagtaaaatCTGTGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCACAAAAAAGCCACCAACCACAAAAAAGAc                      >  1:2718695/11‑100 (MQ=18)
                                                                              aaCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGAc                      <  2:1415092/100‑1 (MQ=255)
                                                                                cTTCTCTGTCTATGAGAGCCGTTAAAATGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACaa                    <  2:4340472/100‑1 (MQ=255)
                                                                                   ctctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATa                 <  2:1714252/100‑1 (MQ=255)
                                                                                    tctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAc                >  2:486135/1‑100 (MQ=255)
                                                                                    tctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAc                >  1:2337155/1‑100 (MQ=255)
                                                                                    tctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAc                >  2:3954276/1‑100 (MQ=255)
                                                                                    aaaatCTATGAGAGTCGTTTTAACGGCTCTCATCGATTTTACTAATAGCAAAATATAAACCGTCCCCTAAAAAGCCACCAACCACAAACCAGACAAATa                 >  1:74506/5‑99 (MQ=25)
                                                                                      tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCAACCAACCACAAACCAGACAAATACcg              >  2:1655936/1‑100 (MQ=255)
                                                                                       gTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcga             >  2:2269313/1‑100 (MQ=255)
                                                                                       gTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcga             <  1:2375840/100‑1 (MQ=255)
                                                                                       gTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcga             >  2:1877854/1‑100 (MQ=255)
                                                                                        tCTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag            >  2:2513603/1‑100 (MQ=38)
                                                                                        tCTATGAGAGCCGTTAAAACGACTCTCATAGAGTTTACTAATAGCAAAATATAAACCGTACCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag            <  1:170343/100‑1 (MQ=255)
                                                                                         cTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc           >  2:3482282/1‑100 (MQ=38)
                                                                                          tATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg          >  2:671630/1‑100 (MQ=38)
                                                                                          tATGAGACCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCAAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc           <  1:1022858/99‑1 (MQ=255)
                                                                                           aTGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTCATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg          >  2:3100909/1‑99 (MQ=38)
                                                                                           aTGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga         >  2:190723/1‑100 (MQ=38)
                                                                                            tGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag        >  1:3848345/1‑100 (MQ=38)
                                                                                            tGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag        >  2:1032712/1‑100 (MQ=255)
                                                                                             gatagCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCgaga       >  2:4265038/4‑100 (MQ=255)
                                                                                             gagaGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCgaga       >  2:3123659/1‑100 (MQ=255)
                                                                                             gagaGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCgaga       >  1:3283295/1‑100 (MQ=255)
                                                                                             gagaGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTACCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCgaga       >  1:2920255/1‑100 (MQ=255)
                                                                                                 gCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAATAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATAc   <  1:428400/100‑1 (MQ=255)
                                                                                                 gCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATAc   >  1:503536/1‑100 (MQ=255)
                                                                                                  ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACa  <  1:1567190/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
GAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACA  >  NC_000913/3171754‑3171951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: