BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,182,712	G→A	69.8%	A55T (GCC→ACC)	zupT →	zinc transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,182,712	0	G	A	69.8%	211.2 / 122.8	168	A55T (GCC→ACC)	zupT	zinc transporter
Reads supporting (aligned to +/- strand): ref base G (23/28); new base A (59/57); total (83/85)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 169 total aligned reads displayed.

GGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACA  >  NC_000913/3182613‑3182809

 gCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGc                                                                                                  <  2:568359/100‑1 (MQ=255)

  cgatctcGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGcc                                                                                                 <  2:3320312/97‑1 (MQ=255)

  cGTTCTCGGGCAAAAACCCTCGAGCCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTAcc                                                                                                 <  1:1068186/100‑1 (MQ=255)

  cGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGcc                                                                                                 <  1:4276052/100‑1 (MQ=255)

  cGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGcc                                                                                                 <  2:2554255/100‑1 (MQ=255)

     tctcGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCa                                                                                              >  1:3387648/1‑100 (MQ=255)

      ctcGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCAc                                                                                             >  1:2457014/1‑100 (MQ=255)

      ctcGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCAc                                                                                             <  2:143918/100‑1 (MQ=255)

      ctcGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGATCATCTCCTTAATGGAAATGCTTCCTGCCGCAc                                                                                             >  1:778123/1‑100 (MQ=255)

        cGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTa                                                                                           <  2:2878940/100‑1 (MQ=255)

         gggCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTag                                                                                          >  2:3688303/1‑100 (MQ=255)

          ggCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGCAATGCTTCCTACCGCACTagc                                                                                         >  1:276630/1‑100 (MQ=255)

          ggCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTagc                                                                                         >  2:108708/1‑100 (MQ=255)

          ggCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTagc                                                                                         >  2:901287/1‑100 (MQ=255)

            cAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTagcag                                                                                       <  2:1120712/100‑1 (MQ=255)

            cAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTagcag                                                                                       >  1:1455446/1‑100 (MQ=255)

            cAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTagcag                                                                                       >  1:3152308/1‑100 (MQ=255)

              aaaaCCCTCGAACCGCTTACGGGCTGTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTagcagc                                                                                      <  1:2508990/99‑1 (MQ=255)

                aaCCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGAGGGGATCATGTTGCTAATCTCATTAATGGGAATGCTTCCTACCGGACTagcag                                                                                       >  1:1070124/1‑96 (MQ=255)

                   cctaaaaCCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGAAATCATGTTGCTCATATCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAg                                                                                 <  2:199024/94‑1 (MQ=255)

                   ccTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGGCGCAATAGCAGCTGAAg                                                                                 >  1:3420938/1‑99 (MQ=255)

                   ccTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAgg                                                                                >  2:1050915/1‑100 (MQ=255)

                   ccTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAgg                                                                                <  1:2857909/100‑1 (MQ=255)

                     tCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGCTCATGTTGCTCATCTCATTACTGGAAATGCTTCCTACCGCACTAGCAGCTGACGGa                                                                               >  1:288792/1‑99 (MQ=255)

                       gAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATg                                                                            >  1:2343472/1‑100 (MQ=255)

                            gCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGcc                                                                       <  1:2854124/100‑1 (MQ=255)

                              tttcTGTCGTTTTCGCTATGTTTTGCGTGTGGGATCATGTTGCTGATCTCATTAATGGAAATGATTCCTACCGCACTCGCAGCTGAAGGAATGTCg                                                                         <  1:4094391/93‑1 (MQ=255)

                               tACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCtgt                                                                    >  2:119881/1‑100 (MQ=255)

                                 cTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTgtgt                                                                  >  2:293554/1‑100 (MQ=255)

                                 cTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCtgtg                                                                   >  1:1990222/1‑99 (MQ=255)

                                 cTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTgtgt                                                                  >  2:3478762/1‑100 (MQ=255)

                                   ggCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTg                                                                >  2:2201855/1‑100 (MQ=255)

                                   ggCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTg                                                                >  2:3640238/1‑100 (MQ=255)

                                    gCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTgg                                                               <  2:1354261/100‑1 (MQ=255)

                                    gCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTgg                                                               <  1:3640213/100‑1 (MQ=255)

                                     cGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTggg                                                              <  2:664666/100‑1 (MQ=255)

                                      gTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGt                                                             <  1:3202375/100‑1 (MQ=255)

                                      gTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGt                                                             <  2:3170390/100‑1 (MQ=255)

                                       ttttCGCTAGGTTTTGCGGCGGGGTTCAGGTTGCTCAGCTCATTAATGGACATGCTTCCTACCGCACTAGCAGCTGAAGGATTTTCGCCTGTTTTggg                                                              <  1:896008/98‑1 (MQ=255)

                                       ttttCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGtn                                                            <  1:1935331/100‑2 (MQ=255)

                                           cGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATgg                                                        <  1:108708/100‑1 (MQ=255)

                                           cGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATgg                                                        >  1:1661465/1‑100 (MQ=255)

                                           cGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATgg                                                        <  2:1924805/100‑1 (MQ=255)

                                            gCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTCGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGa                                                       <  2:3113304/100‑1 (MQ=255)

                                            gCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGa                                                       >  2:3421512/1‑100 (MQ=255)

                                              tAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAAt                                                     <  2:1212595/100‑1 (MQ=255)

                                              tAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAAt                                                     <  1:1776837/100‑1 (MQ=255)

                                              tAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAAGGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAAt                                                     <  1:671383/100‑1 (MQ=255)

                                               aGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATg                                                    <  2:42808/100‑1 (MQ=255)

                                                ggTTTTGCGGCGGCGATCATGTTGCTCATCTCATTAATGGAAATGCTTACTACCGCACTAGCAGCTGAAGGAATGTCGCCTGCGTTGGGTTATGGAATGt                                                   >  1:2729059/1‑100 (MQ=255)

                                                 gTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGtt                                                  <  2:643337/100‑1 (MQ=255)

                                                 gTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGtt                                                  >  1:3483900/1‑100 (MQ=255)

                                                  ttttGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGttt                                                 >  1:2044806/1‑100 (MQ=255)

                                                    ttGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTa                                                <  2:511013/99‑1 (MQ=255)

                                                    ttGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTAt                                               <  2:1541294/100‑1 (MQ=255)

                                                    ttGCCGCGGGGAACATGTTGCTCATTTCATTAATTGAAATGCTTCCTGCCGCACTAGCATCTGAAGTAATGTCGCCTGTGTTGGTTTATGGAATGTTTAt                                               <  1:1229211/100‑1 (MQ=255)

                                                     tGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATc                                              >  1:4049753/1‑100 (MQ=255)

                                                     tGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATc                                              >  1:1261056/1‑100 (MQ=255)

                                                       cggcggGGATCATGTTGCTCATGTCATTAATGGAGATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTAATGTAATGTTTATCtt                                            >  1:2197671/1‑100 (MQ=255)

                                                       cggcggGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCtt                                            >  2:4158318/1‑100 (MQ=255)

                                                       cggcggGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCtt                                            >  2:3774590/1‑100 (MQ=255)

                                                          cggGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCgg                                         <  1:1589777/100‑1 (MQ=255)

                                                             ggaccATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCt                                      <  2:1815435/96‑1 (MQ=255)

                                                             ggATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCt                                      >  1:1775513/1‑100 (MQ=255)

                                                             ggATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCgg                                         <  1:526456/97‑1 (MQ=255)

                                                              gATCATGTTGCTCATCGCATTAATGGAAATGCTTGCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCt                                             <  1:1823020/92‑1 (MQ=255)

                                                                tCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCt                                   >  1:1329028/1‑100 (MQ=255)

                                                                 cATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCtt                                  <  1:4300800/100‑1 (MQ=255)

                                                                 cATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCtt                                  >  2:863397/1‑100 (MQ=255)

                                                                   tGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTg                                 >  1:1292486/1‑99 (MQ=255)

                                                                    gTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGc                               <  2:1327960/100‑1 (MQ=255)

                                                                    gTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGc                               >  2:1874492/1‑100 (MQ=255)

                                                                     ttGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCt                              >  2:1731613/1‑100 (MQ=255)

                                                                        ctcatctcatTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTAtt                           >  1:2806733/1‑100 (MQ=255)

                                                                          catctcatTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTAtttt                         >  2:3727838/1‑100 (MQ=255)

                                                                           atctcatTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTg                        <  1:330099/100‑1 (MQ=255)

                                                                              tcatTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTTGGTTATGGCATGTTTATATTCGGTCTGCTTGGCTATTTTg                        >  1:827746/1‑97 (MQ=255)

                                                                              tcatTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGcc                     <  1:3033944/100‑1 (MQ=255)

                                                                              tcatTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGcc                     <  2:2745727/100‑1 (MQ=255)

                                                                              tcatTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGcc                     <  2:1057188/100‑1 (MQ=255)

                                                                               catTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCt                    >  2:270130/1‑100 (MQ=255)

                                                                               catTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCt                    >  1:3794540/1‑100 (MQ=255)

                                                                               catTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCt                    >  1:188798/1‑100 (MQ=255)

                                                                                 ttAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTgg                  <  1:3925467/100‑1 (MQ=255)

                                                                                 ttAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTgg                  >  1:1188446/1‑100 (MQ=255)

                                                                                  tAATGGAAATGCTTACTGCCGCACTAGCAGCAGAAGGAATGTCGCCTGTGTTGGGTTATGGCATGTTTATATTAGGTCTGCTTGGCTATTTTAGc                      >  1:3943070/1‑95 (MQ=255)

                                                                                   aaTGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCt                    >  1:3824442/1‑96 (MQ=255)

                                                                                    aTGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGAcc               <  2:1022190/100‑1 (MQ=255)

                                                                                      ggAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGc             >  2:2260403/1‑100 (MQ=255)

                                                                                         aaTGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATg          <  1:3727812/100‑1 (MQ=255)

                                                                                          aTGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGt         <  2:267437/100‑1 (MQ=255)

                                                                                            gCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTg       <  1:119881/100‑1 (MQ=255)

                                                                                             cTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGc      <  2:2509632/100‑1 (MQ=255)

                                                                                              ttCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGcc     <  2:397831/100‑1 (MQ=255)

                                                                                               tCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCca    <  2:3483923/100‑1 (MQ=255)

                                                                                               tCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCca    >  2:442697/1‑100 (MQ=255)

                                                                                               tCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCca    <  2:716436/100‑1 (MQ=255)

                                                                                                 cTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCcaca  >  1:3612074/1‑100 (MQ=255)

                                                                                                 cTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCcaca  >  1:222347/1‑100 (MQ=255)

                                                                                                  tGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCCTGTTGcc     <  1:2632494/96‑1 (MQ=255)

GGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACA  >  NC_000913/3182613‑3182809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`