Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,322,705 C→T 16.9% intergenic (‑200/+28) secG ← / ← glmM preprotein translocase membrane subunit/phosphoglucosamine mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,322,7050CT16.9% 413.0 / 63.0 171intergenic (‑200/+28)secG/glmMpreprotein translocase membrane subunit/phosphoglucosamine mutase
Reads supporting (aligned to +/- strand):  ref base C (70/72);  new base T (13/16);  total (83/88)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 181 total aligned reads displayed.

AGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCAC  >  NC_000913/3322607‑3322804
                                                                                                  |                                                                                                   
aGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATcgcc                                                                                                    <  2:1755271/100‑1 (MQ=255)
 gCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATcgccg                                                                                                   <  1:2114765/100‑1 (MQ=255)
   gAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATcgccgc                                                                                                  >  1:1744971/1‑99 (MQ=255)
         ccTTCGCAAGGGCAATTTTATCGCATTTTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTa                                                                                           <  2:534092/100‑1 (MQ=255)
           ttCGCAAGGGCAATTTTATCGCATTGTATTAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGGCGATTGTCGCCttttttct                                                                                         <  1:2175479/100‑4 (MQ=37)
           ttCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGc                                                                                         <  2:47162/100‑1 (MQ=255)
            tCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGGCGATTGTCGCCttttttctc                                                                                        <  2:1044228/100‑5 (MQ=37)
            tCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGGCGATTGTCGCCttttttctc                                                                                        <  1:3993386/100‑5 (MQ=38)
            tCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGcc                                                                                        <  2:3177519/100‑1 (MQ=255)
              gCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGGCGATTGTCGCCttttttctcag                                                                                      <  2:924849/100‑7 (MQ=37)
              gCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGGCGATTGTCGCCttttttctcag                                                                                      <  2:838357/100‑7 (MQ=37)
               cAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGGCGATTGTCGCCttttttctcag                                                                                      <  1:1588784/99‑7 (MQ=37)
                aaggcaaaTTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAg                                                                                      <  1:1602032/92‑1 (MQ=255)
                 aGGACAATTTTATCGCATTGTATCAACTGCGAAAAAAAACAGCAAAACCGATGTGTTCGCTTAGAAAAAAGGCGACAATCGCCGCTTTTTTAGCCAGtt                                                                                    <  1:2719558/99‑1 (MQ=255)
                  gggCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTAt                                                                                  >  1:3352196/1‑100 (MQ=255)
                  gggCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTAt                                                                                  >  1:3533444/1‑100 (MQ=255)
                  gggCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTAt                                                                                  >  2:1477979/1‑100 (MQ=255)
                   ggCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATc                                                                                 >  2:4303328/1‑100 (MQ=255)
                    gCAATTTTATCGCATTGTATCAACTGCGGAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTa                                                                               <  2:3495742/100‑1 (MQ=255)
                     cAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGGAAAAAGCCGACAATCGCCGCCTTTTTAGCCAGTTATCTa                                                                               >  1:3099451/1‑100 (MQ=255)
                        ttttATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACg                                                                            <  2:4049186/100‑1 (MQ=255)
                         tttATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGc                                                                           >  2:1173554/1‑100 (MQ=255)
                         tttATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGc                                                                           <  2:2521078/100‑1 (MQ=255)
                          ttATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGca                                                                          >  1:2521065/1‑99 (MQ=255)
                          ttATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCt                                                                          <  1:314197/100‑1 (MQ=255)
                           tATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCtt                                                                         <  1:4571/100‑1 (MQ=255)
                            aTCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCttt                                                                        >  2:323348/1‑100 (MQ=255)
                             tCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCTATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTa                                                                       >  1:2219264/1‑100 (MQ=255)
                             tCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTa                                                                       <  2:1193518/100‑1 (MQ=255)
                             tCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTa                                                                       >  1:4136633/1‑100 (MQ=255)
                             tCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTa                                                                       <  2:1995862/100‑1 (MQ=255)
                              cGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTa                                                                       <  1:3555793/99‑1 (MQ=255)
                               gCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTATTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATTTAACGCTTTaaa                                                                     >  1:2437037/1‑100 (MQ=255)
                                cATTGTATCAACTGCGGAAAAAAACAGCAAAACGTGTGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAAc                                                                    <  1:2305023/100‑1 (MQ=255)
                                    gTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCt                                                                >  2:1429563/1‑100 (MQ=255)
                                     tATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCtt                                                               <  2:2590480/100‑1 (MQ=255)
                                      aTCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCttt                                                              >  2:1214287/1‑100 (MQ=255)
                                       tCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCtttt                                                             >  1:2281388/1‑100 (MQ=255)
                                        cAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCtttt                                                             <  1:2230685/99‑1 (MQ=255)
                                         aaCTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTAc                                                           >  1:1014423/1‑100 (MQ=255)
                                          aCTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACt                                                          <  2:1576633/100‑1 (MQ=255)
                                           ctacGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAACCAGTTATCTAACGCTTTAAACGGCTTTTACTg                                                         <  1:2910159/97‑1 (MQ=255)
                                            tGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGc                                                        <  1:1411915/100‑1 (MQ=255)
                                             gCGGCAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGAAAATAGCAGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTATTACTGc                                                        >  1:657658/1‑99 (MQ=255)
                                             gCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGc                                                        <  1:3190837/99‑1 (MQ=255)
                                             gCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCa                                                       >  2:390243/1‑100 (MQ=255)
                                                 aaaaaaaCAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCgg                                                   >  1:262014/1‑100 (MQ=255)
                                                  aaaaaaCAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGc                                                  <  2:1894213/100‑1 (MQ=255)
                                                  aaaaaaCAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGc                                                  <  2:2123779/100‑1 (MQ=255)
                                                  aaaaaaCAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTACATCGGc                                                  <  1:612787/100‑1 (MQ=255)
                                                   aaaaaCAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCg                                                 <  1:149802/100‑1 (MQ=255)
                                                     aaaCAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGAt                                               >  2:1675286/1‑100 (MQ=255)
                                                     aaaCAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTCTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGAt                                               <  1:2451478/100‑1 (MQ=255)
                                                       aCAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGc                                             <  1:4327707/100‑1 (MQ=255)
                                                          gCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCTGCGATGCg                                            <  1:3019095/98‑1 (MQ=255)
                                                          gCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGgt                                          >  2:3726520/1‑100 (MQ=255)
                                                          gCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAAGCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGCCGATGCAgt                                          <  2:4111471/100‑1 (MQ=255)
                                                           cAAAACCGATGTGTTCGCTGAGAAAAAATGCGACAATCGCCGCCTTTTTAGCCAGATATCTAACACATTAAACGGCTTTTACTGCATCGACGATGc                                             >  1:700449/1‑96 (MQ=255)
                                                           cAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGCCGATGCAgta                                         <  1:380021/100‑2 (MQ=255)
                                                             aaaCCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGtgtg                                       >  1:1899315/1‑100 (MQ=255)
                                                             aaaCCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGCCGATGcagtaaa                                       <  2:4020954/100‑7 (MQ=255)
                                                              aaCCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGc                                      <  2:552979/100‑1 (MQ=255)
                                                              aaCCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGc                                      <  2:1601655/100‑1 (MQ=255)
                                                               aCCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGc                                      <  1:1860438/99‑1 (MQ=255)
                                                               aCCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCa                                     >  1:803938/1‑100 (MQ=255)
                                                                ccGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCaa                                    >  1:3842735/1‑100 (MQ=255)
                                                                 cGATGTGTTCGCTGAGAAAAAATGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCaaa                                   <  2:4376670/100‑1 (MQ=255)
                                                                 cGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCaaa                                   <  2:2534227/100‑1 (MQ=255)
                                                                 cGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCaaa                                   <  2:3892626/100‑1 (MQ=255)
                                                                  gATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAAt                                  <  1:1377365/100‑1 (MQ=255)
                                                                   aTGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAAtt                                 <  1:1931995/100‑1 (MQ=255)
                                                                   aTGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAAtt                                 <  2:1838219/100‑1 (MQ=255)
                                                                      tgtTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCTGCGATGCGGTGTGCAAATTCAg                              <  1:3057715/100‑1 (MQ=255)
                                                                      tgtTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCac                              <  2:674776/100‑2 (MQ=255)
                                                                      tgtTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAg                              <  2:1550516/100‑1 (MQ=255)
                                                                      tggtCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAg                              <  1:1600164/97‑1 (MQ=255)
                                                                        ttCGCTGAGAAAAAAGGCGACAATCGCCGCCTGTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGTTGTGCAAATTc                                <  1:2000294/96‑1 (MQ=255)
                                                                         tCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTc                            <  1:4137073/99‑1 (MQ=255)
                                                                           actggctaaaaaGGCGGCGATTGTCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCAcc                         >  2:1358933/8‑100 (MQ=37)
                                                                            cTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCt                        >  2:2044946/1‑100 (MQ=255)
                                                                            cTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCt                        >  1:268767/1‑100 (MQ=255)
                                                                            cTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCt                        >  1:1788920/1‑100 (MQ=255)
                                                                               gctaaaaaGGCGGCGATTGTCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTgcg                     >  2:6497/4‑100 (MQ=38)
                                                                               gctaaaaaGGCGGCGATTGTCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTgcg                     >  2:1136439/4‑100 (MQ=38)
                                                                               gctaaaaaGGCGGCGATTGTCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTgcg                     >  2:3763798/4‑100 (MQ=38)
                                                                               agaAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTgcg                     >  1:3919104/1‑100 (MQ=255)
                                                                               agaAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTg                       >  1:2671024/1‑98 (MQ=255)
                                                                                ctaaaaaGGCGGCGATTGTCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTgcg                     >  1:569705/3‑99 (MQ=38)
                                                                                 taaaaaGGCGGCGATTGTCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCt                   >  2:2817250/2‑100 (MQ=38)
                                                                                 taaaaaGGCGGCGATTGTCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCt                   >  1:1150610/2‑100 (MQ=38)
                                                                                  aaaaaGGCGGCGATTGTCGCCTTTTTAGCCAGTTATATAACGCTTTAAACGGCTTTTACTGCATCGACGATGCGGTGTGCAAATCCAGTCACCTgc                      >  1:1694742/1‑96 (MQ=37)
                                                                                  aaaaaGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTACAAATTCAGTCACCTGCGCtt                  <  1:1227890/100‑1 (MQ=255)
                                                                                    aaaGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCg                <  2:2476309/100‑1 (MQ=255)
                                                                                      aGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTc              >  1:1369398/1‑100 (MQ=255)
                                                                                        gCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCtt            >  1:3927646/1‑100 (MQ=255)
                                                                                           aCAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGc         >  2:1655184/1‑100 (MQ=255)
                                                                                            cAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGcc        >  1:1325262/1‑100 (MQ=255)
                                                                                              aTCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCtt      >  1:4180964/1‑100 (MQ=255)
                                                                                               tCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTc     >  1:234553/1‑100 (MQ=255)
                                                                                                 gccgccTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTcca   >  1:1054959/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
AGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCAC  >  NC_000913/3322607‑3322804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: