Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,352,673 T→C 100% E118G (GAA→GGA)  arcB ← aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,352,6730TC99.1% 658.3 / ‑5.0 197E118G (GAA→GGA) arcBaerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base C (99/95);  total (99/98)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 199 total aligned reads displayed.

GAGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTTCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGA  >  NC_000913/3352576‑3352768
                                                                                                 |                                                                                               
gAGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTccc                                                                                               <  1:4006039/100‑1 (MQ=255)
 aGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCt                                                                                              <  2:2314143/100‑1 (MQ=255)
 aGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCt                                                                                              >  2:1754023/1‑100 (MQ=255)
  ncTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTg                                                                                             >  1:123648/2‑100 (MQ=255)
  gCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTg                                                                                             <  2:2886216/100‑1 (MQ=255)
  gCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTg                                                                                             <  1:102479/100‑1 (MQ=255)
  gCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTg                                                                                             <  2:596825/100‑1 (MQ=255)
   cTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGa                                                                                            >  2:700317/1‑100 (MQ=255)
       aTTTGTGTTTCTTCGGCCACTTTGAATTCTATTTTCGGTTGGTTGAAGGTTTCCTGTAGTTCTGCTTCCGCTTTTTCAAGAACGTCAATTCCCAGAttt                                                                                         <  1:1826870/99‑1 (MQ=255)
       aTTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTa                                                                                        >  1:3403863/1‑100 (MQ=255)
        tttGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGATTCCGCTGTTGCACGAACGGCAATTCCCTGATTTAg                                                                                       <  1:715598/100‑1 (MQ=255)
         ttGTTTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGc                                                                                      <  1:1854128/100‑1 (MQ=255)
         ttGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGc                                                                                      <  2:568799/100‑1 (MQ=255)
         ttGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGc                                                                                      <  1:2103802/100‑1 (MQ=255)
          tgtgTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCt                                                                                     >  1:3333707/1‑100 (MQ=255)
          tgtgTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCt                                                                                     >  2:1132601/1‑100 (MQ=255)
          tgtgTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCt                                                                                     >  2:847096/1‑100 (MQ=255)
           gtgtCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTg                                                                                    <  2:1197962/100‑1 (MQ=255)
           gtgtCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTg                                                                                    <  2:3474702/100‑1 (MQ=255)
            tgtCTCTTCGCGGTCTTTGATTTCAATTTTCAGTTGGCCGAGGGTGTCCTGCAGTTCTGCTTCCGCTTTTTCAGGACCGGTAATTCCCTGAttt                                                                                         >  1:2431415/1‑94 (MQ=255)
            tgtCTCTTCGCGCTCTTTGATTTCACTTTTCAGTTGGCCGAAGGCTTCCCGCAGCTCTGCTTCCGCTTTTTCACCAACGACAATTCCCTgct                                                                                           >  1:1457794/1‑90 (MQ=255)
             gtCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTggg                                                                                  <  2:1976379/100‑1 (MQ=255)
             gtCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTggg                                                                                  <  1:3049342/100‑1 (MQ=255)
              tctcTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGc                                                                                 <  2:3628624/100‑1 (MQ=255)
              tctcTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGc                                                                                 >  2:1363444/1‑100 (MQ=255)
               ctctTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCa                                                                                >  2:1898574/1‑100 (MQ=255)
               ctctTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCa                                                                                >  2:3303649/1‑100 (MQ=255)
                 ctTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAat                                                                              <  1:201657/100‑1 (MQ=255)
                  ttCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCACTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAata                                                                             <  2:344744/100‑1 (MQ=255)
                   tCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAatat                                                                            <  1:3162163/100‑1 (MQ=255)
                   tCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAatat                                                                            >  2:2599299/1‑100 (MQ=255)
                    cgcgCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAAtatt                                                                           >  1:1583335/1‑100 (MQ=255)
                       gcTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTTCCTGATTTAGCTGGGCAATATTATc                                                                        <  2:3857396/100‑1 (MQ=255)
                       gcTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATc                                                                        <  2:1135409/100‑1 (MQ=255)
                        ctctTCGATTTCAATTTACAGTTGGCCGAAGGTTTCCTGCAGTTATGCTTCCGCTTTTTCACGAACAGCAATTCCCTGATTTTGCTGGGCAATATTATCt                                                                       >  1:2941018/1‑100 (MQ=255)
                          ctTTGATTTCAGTTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCt                                                                       <  1:3554073/98‑1 (MQ=255)
                          ctTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCttt                                                                     >  2:3518088/1‑100 (MQ=255)
                           tttGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCtttt                                                                    >  2:3890571/1‑100 (MQ=255)
                           tttGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCtttt                                                                    >  2:2011945/1‑100 (MQ=255)
                              gatctCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAAc                                                                 <  2:3171664/96‑1 (MQ=255)
                              gATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTCAc                                                                 >  1:1087609/1‑100 (MQ=255)
                              gATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAAc                                                                 <  2:2093352/100‑1 (MQ=255)
                              gATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGAAGTTCTGCTTCCGCTTTTTCACGAACGGCAATCCCCTGATTTAGCTGGGCACTATTATCTTTTaa                                                                  >  1:3215853/1‑99 (MQ=255)
                                tttCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTg                                                               <  2:1674857/100‑1 (MQ=255)
                                tttCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTg                                                               >  2:3617367/1‑100 (MQ=255)
                                  tCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGTACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGa                                                              <  1:2580190/99‑1 (MQ=255)
                                  tCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGaa                                                             >  1:3205111/1‑100 (MQ=255)
                                   cAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAAc                                                            >  2:1489853/1‑100 (MQ=255)
                                    aaTTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCTCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACg                                                           <  1:2353870/100‑1 (MQ=255)
                                     aTTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACg                                                           >  1:3431833/1‑99 (MQ=255)
                                       tttCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTg                                                        <  1:2242022/100‑1 (MQ=255)
                                       tttCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTg                                                        <  1:3582969/100‑1 (MQ=255)
                                       tttCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTg                                                        >  2:2171914/1‑100 (MQ=255)
                                       tttCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTg                                                        <  1:4095688/100‑1 (MQ=255)
                                          cAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAgg                                                     <  1:4158785/100‑1 (MQ=255)
                                           aGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGc                                                    >  1:174266/1‑100 (MQ=255)
                                            gTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCAGGAACTGCAATTCCTTTACTTAGCTGGGCAATATCATCTGTTAACTGAActt                                                          >  1:2632509/1‑91 (MQ=255)
                                             ttGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTc                                                  <  2:4061634/100‑1 (MQ=255)
                                              tGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCa                                                 <  2:4378562/100‑1 (MQ=255)
                                              tGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCa                                                 <  1:2028718/100‑1 (MQ=255)
                                               ggCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCaa                                                >  2:1325818/1‑100 (MQ=255)
                                                gCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCaaa                                               >  1:605237/1‑100 (MQ=255)
                                                gCCGAAGGTTTCCTGCAGTTCTGCTTACGCTTTTTCACGAACGGCAATTCCCTGCTTTCCCTGGGCAATATCATCTTTTCGCTGAACGTTGTGGCt                                                   >  1:3848049/1‑96 (MQ=255)
                                                 ccGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAt                                              >  1:580781/1‑100 (MQ=255)
                                                  cGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtc                                             <  2:2573906/100‑1 (MQ=255)
                                                      ttccgatctcAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgc                                         <  1:395354/91‑1 (MQ=255)
                                                      ggTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgc                                         >  2:2840693/1‑100 (MQ=255)
                                                      ggTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgc                                         <  1:3626774/100‑1 (MQ=255)
                                                      ggTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgc                                         <  1:3800406/100‑1 (MQ=255)
                                                       gTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtctcgct                                        >  1:2850769/1‑100 (MQ=255)
                                                       gTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgct                                        <  1:1680945/100‑1 (MQ=255)
                                                           ccTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgctcgcg                                    >  2:1271292/1‑100 (MQ=255)
                                                            cTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgctcgcgc                                   >  2:991070/1‑100 (MQ=255)
                                                            cTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgctcgcgc                                   >  2:2395692/1‑100 (MQ=255)
                                                             tGCAGTTGTGCTTCCGCTTTTTCTCGAACGGCAATTTCCCGACTGAGCGGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCa                                  <  1:1283726/100‑1 (MQ=255)
                                                             tGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGTAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCTCTCGCGCa                                  >  1:2865722/1‑100 (MQ=255)
                                                             tGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCCATATTATCTTTTAACTGACCGTTGAGGCTCAAAtcgcgctcgcgg                                   >  1:1900641/1‑98 (MQ=255)
                                                             tGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCa                                  <  1:939733/100‑1 (MQ=255)
                                                             tGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCa                                  <  1:498601/100‑1 (MQ=255)
                                                               cAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAAtcgcgcagatcggaa                                >  2:395355/1‑91 (MQ=255)
                                                                      gCTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCa                         >  2:1078659/1‑100 (MQ=255)
                                                                       cTTCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAg                        <  1:3284159/100‑1 (MQ=255)
                                                                        ttCCGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCtcctcc                          <  1:3272494/97‑1 (MQ=255)
                                                                          ccGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGttt                     >  2:632878/1‑100 (MQ=255)
                                                                           cGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGtttt                    >  2:3243657/1‑100 (MQ=255)
                                                                           cGCTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGtttt                    >  1:3006527/1‑100 (MQ=255)
                                                                             cTTTTTCTCGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTg                  <  1:2371558/100‑1 (MQ=255)
                                                                             cTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTg                  <  2:2232555/100‑1 (MQ=255)
                                                                             cTTTTTCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTg                  <  1:3533571/100‑1 (MQ=255)
                                                                              tttttCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGc                 >  1:695449/1‑100 (MQ=255)
                                                                                 ttCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCAcc              >  2:1186523/1‑100 (MQ=255)
                                                                                  tCACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCa             >  1:2760185/1‑100 (MQ=255)
                                                                                   cACGAACGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAg            <  2:3713412/100‑1 (MQ=255)
                                                                                    aCGACCGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGc           <  1:648932/100‑1 (MQ=255)
                                                                                        aCGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTg       <  1:814574/100‑1 (MQ=255)
                                                                                        aCGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTg       <  1:2424219/100‑1 (MQ=255)
                                                                                        aCGGCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTg       <  1:446148/100‑1 (MQ=255)
                                                                                          ggCAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGAc     >  2:482902/1‑100 (MQ=255)
                                                                                           ncAATTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTAGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACa    >  1:2004785/2‑100 (MQ=255)
                                                                                             aaTTCCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGa  >  2:3246979/1‑100 (MQ=255)
                                                                                                 |                                                                                               
GAGCTGAATTTGTGTCTCTTCGCGCTCTTTGATTTCAATTTTCAGTTGGCCGAAGGTTTCCTGCAGTTCTGCTTCCGCTTTTTCACGAACGGCAATTTCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGA  >  NC_000913/3352576‑3352768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: