Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,411,299 T→C 100% V10A (GTA→GCA)  fis → global DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,411,2990TC99.4% 589.9 / ‑4.9 167V10A (GTA→GCA) fisglobal DNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (60/106);  total (60/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

Only 100 of 167 total aligned reads displayed.

AACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACT  >  NC_000913/3411202‑3411395
                                                                                                 |                                                                                                
  cAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTg                                                                                              <  1:1058149/100‑1 (MQ=255)
  cAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTg                                                                                              >  2:2704775/1‑100 (MQ=255)
   aGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGa                                                                                             <  1:500815/100‑1 (MQ=255)
    gCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGAc                                                                                            >  1:4283238/1‑100 (MQ=255)
       ggAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCg                                                                                          <  1:631865/99‑1 (MQ=255)
       ggAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGt                                                                                         >  1:421360/1‑100 (MQ=255)
         aGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGttt                                                                                       <  2:1632558/100‑1 (MQ=255)
         aGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTAAGTTCGAACAACGCGTAAATTCTGACGCACTGACCGttt                                                                                       >  2:1827329/1‑100 (MQ=255)
          ggCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTc                                                                                      <  1:3081155/100‑1 (MQ=255)
          ggCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTc                                                                                      <  2:2292663/100‑1 (MQ=255)
          ggCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTc                                                                                      <  1:147916/100‑1 (MQ=255)
           gCGTTGGAGGCATACTTCTAAAATTTTGCGTAAACAGAAATAAAGAGCTGTCAGAACTATGTTCGAACAACGCCTAAATTCTGACGCACTGACCGTTTCt                                                                                     <  1:472445/100‑1 (MQ=255)
            cGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTa                                                                                    <  1:3390371/100‑1 (MQ=255)
            cGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTa                                                                                    <  2:2380466/100‑1 (MQ=255)
            cGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTa                                                                                    >  2:2334797/1‑100 (MQ=255)
            cGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTa                                                                                    >  2:125199/1‑100 (MQ=255)
            cGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTa                                                                                    >  1:3664679/1‑100 (MQ=255)
              ttGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTAcc                                                                                  <  2:3922846/100‑1 (MQ=255)
              ttGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTAcc                                                                                  <  2:2280332/100‑1 (MQ=255)
               tGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCg                                                                                 <  2:3044530/100‑1 (MQ=255)
               tGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCg                                                                                 <  1:1670951/100‑1 (MQ=255)
                ggAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGt                                                                                <  2:2236348/100‑1 (MQ=255)
                 gAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGCGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGCCGCACTGACCGTTTCTACCGtt                                                                               >  1:352266/1‑100 (MQ=255)
                 gAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGtt                                                                               <  1:778612/100‑1 (MQ=255)
                  aGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTa                                                                              <  2:2926815/100‑1 (MQ=255)
                  aGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTa                                                                              <  1:2288929/100‑1 (MQ=255)
                    gCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAAc                                                                            <  1:1013903/100‑1 (MQ=255)
                     cATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAAct                                                                           >  1:1805747/1‑100 (MQ=255)
                     cATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAAAGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAAc                                                                            <  2:3696402/99‑1 (MQ=255)
                        aCTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACtctc                                                                        <  2:1123643/100‑1 (MQ=255)
                         tctCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCa                                                                       <  2:1327995/98‑1 (MQ=255)
                         cTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCa                                                                       <  2:141439/100‑1 (MQ=255)
                         cTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCa                                                                       <  1:2459736/100‑1 (MQ=255)
                          ttCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAg                                                                      <  2:2478334/100‑1 (MQ=255)
                           tCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAgg                                                                     >  2:707761/1‑100 (MQ=255)
                           tCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAgg                                                                     <  1:2842571/100‑1 (MQ=255)
                            cGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCaaga                                                                    >  1:1327990/1‑97 (MQ=255)
                            cGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGa                                                                    >  1:4132307/1‑100 (MQ=255)
                             gAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGAt                                                                   >  1:3154466/1‑100 (MQ=255)
                               aaaTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCa                                                                 <  2:1821069/100‑1 (MQ=255)
                                 aTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAgg                                                               <  2:1146980/100‑1 (MQ=255)
                                 aTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAgg                                                               <  2:462999/100‑1 (MQ=255)
                                   tttGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTa                                                             <  2:3166557/100‑1 (MQ=255)
                                       cGTAAACAGAAATGAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAcc                                                          <  1:4087901/99‑1 (MQ=255)
                                       cGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAccc                                                         >  1:1388758/1‑100 (MQ=255)
                                       cGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAccc                                                         >  2:2873983/1‑100 (MQ=255)
                                       cGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAccc                                                         <  1:1492605/100‑1 (MQ=255)
                                       cGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAccc                                                         <  1:3817448/100‑1 (MQ=255)
                                       cGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAccc                                                         >  1:4245472/1‑100 (MQ=255)
                                        gTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCa                                                        <  1:3278994/100‑1 (MQ=255)
                                        gTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCa                                                        >  2:4035621/1‑100 (MQ=255)
                                             cAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAc                                                   <  2:950733/100‑1 (MQ=255)
                                                aaaTAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAcccc                                                <  1:3930624/100‑1 (MQ=255)
                                                 aaTAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCt                                               >  2:2191574/1‑100 (MQ=255)
                                                 aaTAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCt                                               >  2:3218719/1‑100 (MQ=255)
                                                  aTAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTg                                              >  2:956249/1‑100 (MQ=255)
                                                  aTAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTg                                              <  2:837312/100‑1 (MQ=255)
                                                    aaaGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCg                                            <  2:1274798/100‑1 (MQ=255)
                                                      agagCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTg                                          <  1:1213456/100‑1 (MQ=255)
                                                      agagCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTg                                          <  1:2614922/100‑1 (MQ=255)
                                                      agagCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTg                                          <  2:938646/100‑1 (MQ=255)
                                                        agCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGAc                                        <  2:4407911/100‑1 (MQ=255)
                                                        agCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGAc                                        >  2:4099447/1‑100 (MQ=255)
                                                         gCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACt                                       >  2:1164198/1‑100 (MQ=255)
                                                         gCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACt                                       >  1:3809500/1‑100 (MQ=255)
                                                          cTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTc                                      >  2:1961132/1‑100 (MQ=255)
                                                            gACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCgg                                    >  2:4337986/1‑100 (MQ=255)
                                                            gACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCgg                                    >  2:2066019/1‑100 (MQ=255)
                                                            gACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCgg                                    >  2:3220820/1‑100 (MQ=255)
                                                             aCAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGt                                   <  2:3510061/100‑1 (MQ=255)
                                                              cAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGtt                                  <  2:886860/100‑1 (MQ=255)
                                                              cAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGtt                                  <  1:1360539/100‑1 (MQ=255)
                                                               aGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTa                                 >  2:2531296/1‑100 (MQ=255)
                                                               aGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTa                                 <  1:1718850/100‑1 (MQ=255)
                                                                gAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTaa                                >  1:1892704/1‑100 (MQ=255)
                                                                gAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTaa                                <  2:1895721/100‑1 (MQ=255)
                                                                  aCTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAAc                              >  2:678335/1‑100 (MQ=255)
                                                                      tgtgCGAACAACGCGTAAATTCTGGCGCACTGAGCGTTTCTACGGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGc                          <  2:2349495/100‑1 (MQ=255)
                                                                      tGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGc                          <  2:4010549/100‑1 (MQ=255)
                                                                       gTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCa                         <  2:3579139/100‑1 (MQ=255)
                                                                            aacaacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaa                    >  2:2870250/1‑100 (MQ=255)
                                                                            aacaacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaa                    <  2:1585186/100‑1 (MQ=255)
                                                                            aacaacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaa                    >  2:1494504/1‑100 (MQ=255)
                                                                             acaacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAGAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaag                   <  2:3115508/100‑1 (MQ=255)
                                                                               aacGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaagat                 <  2:4083469/100‑2 (MQ=255)
                                                                               aacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaagaa                 <  2:3482357/100‑1 (MQ=255)
                                                                                 cgcgTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACt               >  1:2976308/1‑100 (MQ=255)
                                                                                 cgcgTAAATGCTGACGCACTGACCGTTTCTACCGTTAACTCGCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaagaan                <  2:1184973/99‑2 (MQ=255)
                                                                                     tAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTAttt           <  1:282378/100‑1 (MQ=255)
                                                                                       aaTTCTGACGCACTGACCGTTTCTACCGTTATCTCTTAGGATCAGGTAACCCAAATACCCCTGTTTGACTCGGTTAAACAGGCACTGAAGAACTATTTTg         <  1:3977240/100‑1 (MQ=255)
                                                                                       aaTTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAGAAACCCCTGCGTGCCTCGGTTAAACAGGCACTGAAGAACTAtttt          >  1:642961/1‑99 (MQ=255)
                                                                                        aTTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGc        <  2:4245504/100‑1 (MQ=255)
                                                                                        aTTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGc        >  2:1177261/1‑100 (MQ=255)
                                                                                         ttCTGACCCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCt       >  2:757927/1‑100 (MQ=255)
                                                                                          tCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTc      <  2:3771303/100‑1 (MQ=255)
                                                                                            tGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCaa    >  1:1653692/1‑100 (MQ=255)
                                                                                            tGACGCACTGACAGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCa     <  1:1989800/99‑1 (MQ=255)
                                                                                             gACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAAc   >  1:1853341/1‑100 (MQ=255)
                                                                                             gACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAAc   >  2:3264900/1‑100 (MQ=255)
                                                                                              aCGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACt  <  1:3739530/100‑1 (MQ=255)
                                                                                                 |                                                                                                
AACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACT  >  NC_000913/3411202‑3411395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑