BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	410,479	A→G	100%	S112S (TCA→TCG)	mak →	manno(fructo)kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	410,479	0	A	G	98.8%	599.4 / ‑3.6	175	S112S (TCA→TCG)	mak	manno(fructo)kinase
Reads supporting (aligned to +/- strand): ref base A (2/1); new base G (90/82); total (92/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

AACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGC  >  NC_000913/410383‑410574

aaCCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGaa                                                                                              >  1:4193446/1‑100 (MQ=255)

  ccTGGCTCAACGGTCAGCCATTCGATTAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGc                                                                                            >  2:2693210/1‑100 (MQ=255)

  ccTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGGGGTTTCGGAAGc                                                                                            >  1:1634354/1‑100 (MQ=255)

  ccTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGc                                                                                            >  2:1266138/1‑100 (MQ=255)

  ccTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGc                                                                                            <  2:4323681/100‑1 (MQ=255)

  ccTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGc                                                                                            <  2:3933044/100‑1 (MQ=255)

  ccTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGc                                                                                            <  2:3690696/100‑1 (MQ=255)

  ccTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGc                                                                                            <  1:3171259/100‑1 (MQ=255)

  ccTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGc                                                                                            <  1:2547408/100‑1 (MQ=255)

   cTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGCCGCTAACTGGCTGGCGGTTTCGGAAGCa                                                                                           >  1:4380492/1‑100 (MQ=255)

   cTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCa                                                                                           <  1:3479480/100‑1 (MQ=255)

    tGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAg                                                                                          <  2:1593499/100‑1 (MQ=255)

     ggCTCAACGTTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGt                                                                                         <  1:136906/100‑1 (MQ=255)

      gCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGTGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTa                                                                                        <  2:261793/100‑1 (MQ=255)

      gCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGtn                                                                                        <  1:794690/100‑2 (MQ=255)

      gCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTa                                                                                        >  1:26553/1‑100 (MQ=255)

      gCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTa                                                                                        >  1:2859587/1‑100 (MQ=255)

       cTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAg                                                                                       >  2:1476770/1‑100 (MQ=255)

       cTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAg                                                                                       >  2:712555/1‑100 (MQ=255)

        tCAACGGTCAGCCATTCGATAAAGAATTACGCGCGGGGTTGCAGCGGGAAGTGCGGCCGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGa                                                                                      <  2:2629578/100‑1 (MQ=255)

         cAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGAt                                                                                     >  1:3618088/1‑100 (MQ=255)

            cGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGgc                                                                                  <  2:2072364/100‑1 (MQ=255)

               tCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGATGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGcgcg                                                                               <  1:3090784/100‑1 (MQ=255)

                 aGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGc                                                                             >  2:3050766/1‑100 (MQ=255)

                  gCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCa                                                                            >  1:548511/1‑100 (MQ=255)

                   ccATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAg                                                                           >  2:4031448/1‑100 (MQ=255)

                    cATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGc                                                                          >  2:3786108/1‑100 (MQ=255)

                      ttCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGACTGGCAAATGACGCTAACTGTCTGGAGGTTTCAGAAGCAGTAGCTGGCGCGGCa                                                                            >  1:369778/1‑96 (MQ=255)

                        cGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGa                                                                      >  2:3770537/1‑100 (MQ=255)

                         gATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCggg                                                                       >  1:2158622/1‑98 (MQ=255)

                         gATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAg                                                                     >  2:2864718/1‑100 (MQ=255)

                         gATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAg                                                                     >  1:277449/1‑100 (MQ=255)

                         gATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAg                                                                     >  1:1626697/1‑100 (MQ=255)

                          aTAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAgc                                                                    <  1:1662437/100‑1 (MQ=255)

                             aagccgtAAGCGCGAGGTTGTAGCTGGAAGAGAAGCTGGCGAATGACGCGAAATGTCTGGCGGGTTAGGAAGCAGTAGATGGCGCGGCAGCGGGAgcgc                                                                  <  1:1842561/93‑1 (MQ=255)

                             aaGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCGGGCAAGTGACGCTGACTGTCTGGGGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCa                                                                 <  1:123998/100‑1 (MQ=255)

                                aCTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGAc                                                              <  1:1564923/100‑1 (MQ=255)

                                 cTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACg                                                             <  1:4017676/100‑1 (MQ=255)

                                 cTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACg                                                             >  2:1435145/1‑100 (MQ=255)

                                     aGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTAt                                                         <  2:60571/100‑1 (MQ=255)

                                       cgcgAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTAttt                                                       >  2:1249836/1‑100 (MQ=255)

                                       cgcgAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTAt                                                         >  1:137168/1‑98 (MQ=255)

                                        gcgAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTAttt                                                       <  1:3934439/99‑1 (MQ=255)

                                         cgAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGc                                                     <  2:4021080/100‑1 (MQ=255)

                                          gAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGcc                                                    >  2:3936102/1‑100 (MQ=255)

                                            ggTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGt                                                  <  1:357928/100‑1 (MQ=255)

                                            ggTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGt                                                  <  2:1821593/100‑1 (MQ=255)

                                            ggTTGCAGCGGGAAGTGCGGCGGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGt                                                  <  1:1919861/100‑1 (MQ=255)

                                             gTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTg                                                 <  2:2762629/100‑1 (MQ=255)

                                             gTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTg                                                 <  2:1315744/100‑1 (MQ=255)

                                              ttGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCTGGAGCGCAGACGGTATTTGCCGTg                                                 <  1:4150770/99‑1 (MQ=255)

                                               tGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGa                                                >  1:3294577/1‑99 (MQ=255)

                                               tGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGAt                                               >  2:2262368/1‑100 (MQ=255)

                                               tGCAGCGGGAAGTGCGGCTGGCAAATGACGATCAGAGTCGGGCCGTGTCGGAAGCGGGAGAGGCCGTGGGTGCGGGACGGTAGACGGTAtat                                                       >  1:3845729/1‑90 (MQ=255)

                                                gCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGAtt                                              >  2:2436004/1‑100 (MQ=255)

                                                 cAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTa                                             >  2:2602012/1‑100 (MQ=255)

                                                    cGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGCGCGCAGACGGAATTTGCCGTGATTATCg                                          >  2:3278962/1‑100 (MQ=255)

                                                      ggAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGc                                        >  2:3599370/1‑100 (MQ=255)

                                                       gAAGTGCGGCTGGCAAATGCGCCTACATGTCTGGCGGATGCGGAAGCAGTAGATGTCGCAGCACGGGGGGCGCAGACGGTATTTAGCGTGATTCTCgtc                                        >  1:1076881/1‑97 (MQ=255)

                                                       gAAGTGCGGCTGGCAAATGACGCTACCTGTCCGGCGGTTTCGGCAGCAGTAGATGGCCCGGCATCGTGAGCGCAGACGGTATTTGCCATGATTATGGGCa                                       >  1:1076752/1‑100 (MQ=255)

                                                         aGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACg                                     <  1:2525436/100‑1 (MQ=255)

                                                          gTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGAGCAGACGGTATTTGCCGTGATTATCGGCa                                       >  1:4379528/1‑97 (MQ=255)

                                                            gCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGa                                  <  2:4245907/100‑1 (MQ=255)

                                                             cGGCTGGCAAATGACGCTCACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGGCGGTACTTGCCGTGATTATCGGCACGGGAt                                 >  2:1210627/1‑100 (MQ=255)

                                                              ggCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGTGCAGACGGTATTTGCCGTGATTATAGGCACGGGATg                                <  1:2192325/100‑1 (MQ=255)

                                                              ggCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGacg                                >  1:3879764/1‑98 (MQ=255)

                                                              ggCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGAt                                 >  2:3404915/1‑99 (MQ=255)

                                                               gCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATgc                               >  1:640683/1‑100 (MQ=255)

                                                                cTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATgcg                              <  2:1083375/100‑1 (MQ=255)

                                                                cTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATgcg                              >  1:910890/1‑100 (MQ=255)

                                                                  ggCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATgcggc                            >  1:1880275/1‑100 (MQ=255)

                                                                     aaaTGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGcgcg                         <  1:2864619/100‑1 (MQ=255)

                                                                        tGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGAGGGCGGGGCAGCGGGAGCCCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGgtgccg                        <  1:2030957/98‑3 (MQ=255)

                                                                         gACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCg                     <  1:4281042/100‑1 (MQ=255)

                                                                           cGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTg                   >  2:3475625/1‑100 (MQ=255)

                                                                           cGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTg                   >  2:2985971/1‑100 (MQ=255)

                                                                           cGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTg                   <  2:2289357/100‑1 (MQ=255)

                                                                            ccTAACTGTCTGGAGGTTCCGGAAACAGAAGCTCGCGCGGCAGCGGGAGCGACGCCGGTAACTGCCGTGATTATCGGCACGGGAAGAGGCGCgg                        >  1:3736223/2‑94 (MQ=255)

                                                                             cTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGc                 <  1:2985953/100‑1 (MQ=255)

                                                                             cTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGc                 >  2:1939267/1‑100 (MQ=255)

                                                                             cTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGc                 <  1:3404895/100‑1 (MQ=255)

                                                                              tAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCa                >  2:2127752/1‑100 (MQ=255)

                                                                              tAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCa                <  2:355784/100‑1 (MQ=255)

                                                                              tAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCa                >  2:1975366/1‑100 (MQ=255)

                                                                                aCTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGACGCGGGCGTGGCAtt              >  2:4351740/1‑100 (MQ=255)

                                                                                 cTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTc             <  1:2592752/100‑1 (MQ=255)

                                                                                  tGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCa            >  1:3075425/1‑100 (MQ=255)

                                                                                   gTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCaa           <  2:2717469/100‑1 (MQ=255)

                                                                                   gTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCaa           <  1:3119155/100‑1 (MQ=255)

                                                                                     cTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATg         >  1:1646579/1‑100 (MQ=255)

                                                                                     cTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATg         >  1:490265/1‑100 (MQ=255)

                                                                                      tGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgg        >  1:1546258/1‑100 (MQ=255)

                                                                                      tGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCAGGAGCGCAGAGGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgg        <  1:1146844/100‑1 (MQ=255)

                                                                                       ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATggg       >  1:184654/1‑100 (MQ=255)

                                                                                          ggTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcgg    >  2:2354676/1‑100 (MQ=255)

                                                                                           gTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcggg   <  1:3046984/100‑1 (MQ=255)

                                                                                            tttCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcgggc  <  2:3930162/100‑1 (MQ=255)

                                                                                            tttCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcgggc  >  2:3831318/1‑100 (MQ=255)

                                                                                            tttCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcgggc  >  1:3564229/1‑100 (MQ=255)

                                                                                            tttCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcgggc  <  2:1452519/100‑1 (MQ=255)

AACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGC  >  NC_000913/410383‑410574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`