Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,646,678 G→A 24.2% G127D (GGC→GAC)  gor → glutathione oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,646,6780GA24.2% 385.0 / 122.3 211G127D (GGC→GAC) gorglutathione oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (79/81);  new base A (33/18);  total (112/99)
Fisher's exact test for biased strand distribution p-value = 7.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

Only 100 of 214 total aligned reads displayed.

GTATTCATACTTCCTATGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATACGGTATTGATTCTGATGGCT  >  NC_000913/3646579‑3646776
                                                                                                   |                                                                                                  
gTATTCATACTTCCTATGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACgg                                                                                                    <  1:3336283/100‑1 (MQ=255)
 tATTCATACTTCCTATGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGc                                                                                                   <  1:752919/100‑1 (MQ=255)
    tCATACTTCCTATGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGACGaa                                                                                                >  2:2574797/1‑100 (MQ=255)
     cATACTTCCTATGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGaaa                                                                                               >  1:3916686/1‑100 (MQ=255)
     cATACTTCCTATGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCAAAAACGCTGGAGGTAAACGACGaaa                                                                                               >  1:1835843/1‑100 (MQ=255)
      aTACTTCCTATGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAAc                                                                                              >  1:4400631/1‑100 (MQ=255)
      aTACTTCCTATGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAAc                                                                                              >  2:4045240/1‑100 (MQ=255)
         ggaagtATGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCAt                                                                                           >  2:3709456/6‑100 (MQ=255)
          ttCCTATGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATc                                                                                          <  2:4160329/100‑1 (MQ=255)
            ccTATGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCAc                                                                                        >  2:2797519/1‑100 (MQ=255)
            ccTATGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCAc                                                                                        <  2:3681918/100‑1 (MQ=255)
            ccTATGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCAc                                                                                        <  1:2233929/100‑1 (MQ=255)
             cTATGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACg                                                                                       >  2:1142585/1‑100 (MQ=255)
             cTATGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACg                                                                                       >  1:1391691/1‑100 (MQ=255)
             cTATGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACg                                                                                       <  2:2339015/100‑1 (MQ=255)
              tATGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACgg                                                                                      <  2:3083659/100‑1 (MQ=255)
               aTGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGc                                                                                     <  2:3641945/100‑1 (MQ=255)
               aTGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGc                                                                                     >  2:509359/1‑100 (MQ=255)
                tGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGcc                                                                                    >  2:521507/1‑100 (MQ=255)
                  aaaaCGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGa                                                                                  <  2:4286668/100‑1 (MQ=255)
                    aaCGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATc                                                                                <  2:42475/100‑1 (MQ=255)
                    aaCGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATc                                                                                <  1:3879096/100‑1 (MQ=255)
                     ncGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATc                                                                                >  1:2323309/2‑99 (MQ=255)
                     aCGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAACACGCTGGAGGTAAACGACGAAACCATCACGGCCGATCa                                                                               <  1:953323/100‑1 (MQ=255)
                     aCGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCa                                                                               <  1:99206/100‑1 (MQ=255)
                      cgcgCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCat                                                                              <  1:2846195/97‑1 (MQ=255)
                      cGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATCat                                                                              >  1:3715717/1‑100 (MQ=255)
                         gCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATAtt                                                                           >  1:3073289/1‑100 (MQ=255)
                          cTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTACACGGCGAAACCATCACGGCCGATCATATTc                                                                          >  2:573973/1‑100 (MQ=255)
                          cTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTc                                                                          <  2:1378344/100‑1 (MQ=255)
                          cTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTc                                                                          >  1:4143728/1‑100 (MQ=255)
                            cGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTg                                                                        >  1:3002826/1‑100 (MQ=255)
                             ggTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGCGACCATCACGGCCGATCATATTCTGa                                                                       >  1:281901/1‑100 (MQ=255)
                             ggTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGa                                                                       >  2:3789064/1‑100 (MQ=255)
                             ggTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGa                                                                       >  1:2524556/1‑100 (MQ=255)
                               tAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATc                                                                     <  2:1981015/100‑1 (MQ=255)
                               tAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATACTCTGATc                                                                     <  2:2313878/100‑1 (MQ=255)
                                aaaaaTAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCg                                                                    <  2:3364968/100‑1 (MQ=255)
                                 aaaaTAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGc                                                                   <  1:897192/100‑1 (MQ=255)
                                     tAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCcaca                                                               <  1:1193132/100‑1 (MQ=255)
                                       aCGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATCATATTCTGATCGCCACAgg                                                             >  2:3998359/1‑100 (MQ=255)
                                        cGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGc                                                            >  2:2017878/1‑100 (MQ=255)
                                        cGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGc                                                            >  2:849665/1‑100 (MQ=255)
                                        cGTTGATGTAATCAAAGGCTATGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGc                                                            >  1:2688249/1‑100 (MQ=255)
                                            gATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGgtc                                                        >  2:1660441/1‑100 (MQ=255)
                                            gATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCCgtc                                                        >  1:805350/1‑100 (MQ=255)
                                                tAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTcc                                                    >  2:598151/1‑100 (MQ=255)
                                                 aaTCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCg                                                   >  2:2482026/1‑100 (MQ=255)
                                                  aTCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGa                                                  >  1:3171230/1‑100 (MQ=255)
                                                   tCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAg                                                 <  1:2167507/100‑1 (MQ=255)
                                                   tCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAg                                                 <  2:287324/100‑1 (MQ=255)
                                                    cAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGc                                                >  2:3505591/1‑100 (MQ=255)
                                                    cAAAGGCGTTGCCCGCTGCGTTGAGGCCAAACGGCTGGAGGGAAACGGCGGAACCATCACGGCCGAGCATATTCTGATCGCCACAGGCGGTCGTCCGAg                                                 <  1:4219682/99‑1 (MQ=255)
                                                     aaaGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGc                                                <  1:2634326/99‑1 (MQ=255)
                                                      aaGGCTTTGCCCGCTTCGTTGATGACAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCa                                              <  1:1046809/100‑1 (MQ=255)
                                                        ggCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCAcc                                            >  2:1542860/1‑100 (MQ=255)
                                                         gCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCAccc                                           <  2:125787/100‑1 (MQ=255)
                                                           tttGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCgg                                         <  2:2606871/100‑1 (MQ=255)
                                                            ttGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGa                                        <  2:2136848/100‑1 (MQ=255)
                                                             tGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGat                                       >  1:2082711/1‑100 (MQ=255)
                                                             tGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGat                                       >  1:2508243/1‑100 (MQ=255)
                                                              gCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGata                                      >  1:2264535/1‑100 (MQ=255)
                                                              gCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATCATTTTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGata                                      >  1:1443082/1‑100 (MQ=255)
                                                               cccGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACACGCGGTCGTCCGATCCAccc                                           >  1:4000393/1‑94 (MQ=255)
                                                                ccGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCCCCCGGATAtt                                    >  1:2704660/1‑100 (MQ=255)
                                                                  gCTTCGTTTATTCCAAAACGCTGGATGTAAACGGCGAAACCATCACGGCCTATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTcc                                  <  1:2930437/100‑1 (MQ=255)
                                                                  gCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTcc                                  <  2:2282442/100‑1 (MQ=255)
                                                                      cGTTGATGCCAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGc                              >  2:2245358/1‑100 (MQ=255)
                                                                       gTTGATGCCAAAACGCTGGAGGTAAACGGCGACACCATCACGGCCGATCATCTTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCg                             >  2:1466540/1‑100 (MQ=255)
                                                                       gTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCg                             >  2:1850067/1‑100 (MQ=255)
                                                                        ttGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGAATATCCgggtgg                            <  2:3623332/100‑4 (MQ=255)
                                                                         tGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTg                           <  2:61774/100‑1 (MQ=255)
                                                                         tGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTg                           >  2:3526629/1‑100 (MQ=255)
                                                                         tGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGAATATCCgggtggc                           <  2:3888927/100‑5 (MQ=255)
                                                                         tGATGCCAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTg                           >  2:4413070/1‑100 (MQ=255)
                                                                         tGATGCCAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTg                           >  2:2151732/1‑100 (MQ=255)
                                                                           aTGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGa                         <  1:1914651/100‑1 (MQ=255)
                                                                            tGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGaa                        >  1:3662140/1‑100 (MQ=255)
                                                                            tGCCAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGaa                        >  1:130183/1‑100 (MQ=255)
                                                                            tGCCAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGaa                        >  2:242255/1‑100 (MQ=255)
                                                                            tGCCAAAACGCTGGAGGTAAACGACGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGaa                        >  1:3804425/1‑100 (MQ=255)
                                                                              ccAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATa                      >  1:3525615/1‑100 (MQ=255)
                                                                              ccAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATa                      >  1:2800004/1‑100 (MQ=255)
                                                                              ccAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATa                      >  2:1401286/1‑100 (MQ=255)
                                                                                 aaaCGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATACgg                   <  1:2430089/100‑1 (MQ=255)
                                                                                 aaaCGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATACgg                   >  1:1523786/1‑100 (MQ=255)
                                                                                 aaaCGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATACgg                   <  1:2032764/100‑1 (MQ=255)
                                                                                     gCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATACGGTAtt               >  2:270758/1‑100 (MQ=255)
                                                                                     gCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATACGGTAtt               >  1:771456/1‑100 (MQ=255)
                                                                                      cTGGAGGTAAACGACGCAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATACGGTATTg              <  1:2574784/100‑1 (MQ=255)
                                                                                          aGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATACGGTATTGATTc          >  2:1423636/1‑100 (MQ=255)
                                                                                          aGGTAAACGGCGAAACCATCACGGCCGATCAAATTCTGATGGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATACGGTATAGATTc          >  1:488163/1‑100 (MQ=255)
                                                                                          aGGTAAACGACGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATACGGTATTGATTc          >  1:1754401/1‑100 (MQ=255)
                                                                                           ggTAAACGACGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATACGGTATTGATTCt         >  2:2504081/1‑100 (MQ=255)
                                                                                              aaaCGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATACGGTATTGATTCTGAt      <  1:573971/100‑1 (MQ=255)
                                                                                              aaaCGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATACGGTATTGATTCTGAt      <  2:3844776/100‑1 (MQ=255)
                                                                                              aaaCGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATACGGTATTGATTCTGAt      <  2:2207289/100‑1 (MQ=255)
                                                                                              aaaCGACGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATACGGTATTGATTCTGAt      >  2:3155871/1‑100 (MQ=255)
                                                                                               aaCGGCGAAACCATCACGGCCGATCATATTCGGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATACGGTATTGATTCTGATg     <  1:4055691/100‑1 (MQ=255)
                                                                                                  ggCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATACGGTATTGATTCTGATGGct  >  2:333237/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
GTATTCATACTTCCTATGAAAACGTGCTCGGTAAAAATAACGTTGATGTAATCAAAGGCTTTGCCCGCTTCGTTGATGCCAAAACGCTGGAGGTAAACGGCGAAACCATCACGGCCGATCATATTCTGATCGCCACAGGCGGTCGTCCGAGCCACCCGGATATTCCGGGCGTGGAATACGGTATTGATTCTGATGGCT  >  NC_000913/3646579‑3646776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: