Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,920,796 T→C 15.1% A31A (GCA→GCG atpF ← F0 sector of membrane‑bound ATP synthase, subunit b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,920,7960TC15.1% 515.7 / 79.0 219A31A (GCA→GCGatpFF0 sector of membrane‑bound ATP synthase, subunit b
Reads supporting (aligned to +/- strand):  ref base T (91/94);  new base C (14/19);  total (105/114)
Fisher's exact test for biased strand distribution p-value = 5.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-01

Only 100 of 222 total aligned reads displayed.

CTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACA  >  NC_000913/3920697‑3920894
                                                                                                   |                                                                                                  
cTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCACTTTCTTTTTGACGTTTTTCGATGGCt                                                                                                    <  2:2682029/100‑1 (MQ=255)
 tGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCt                                                                                                    >  1:2287543/1‑99 (MQ=255)
 tGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTg                                                                                                   <  2:2292650/100‑1 (MQ=255)
  ggtcggtcGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGc                                                                                                  >  1:3163373/1‑100 (MQ=255)
   gtcggtcgCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGcc                                                                                                 <  1:2805199/100‑1 (MQ=255)
       gtcgCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTa                                                                                             >  1:3628699/1‑100 (MQ=255)
       gtcgCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTa                                                                                             >  2:2041902/1‑100 (MQ=255)
         cgcgCTGGCGTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAAt                                                                                           <  2:4054112/100‑1 (MQ=255)
          gcgcTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAAt                                                                                           >  2:571553/1‑99 (MQ=255)
            gcTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggc                                                                                        >  2:836582/1‑100 (MQ=255)
              tGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGTGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcgg                                                                                      <  2:1897505/100‑1 (MQ=255)
               ggCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcggn                                                                                     <  1:3757837/100‑2 (MQ=255)
               ggCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcggc                                                                                     <  1:2665959/100‑1 (MQ=255)
               ggCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcggc                                                                                     <  2:1499811/100‑1 (MQ=255)
                gCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGcc                                                                                    <  1:1588808/100‑1 (MQ=255)
                  cTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCan                                                                                  <  2:283296/100‑2 (MQ=255)
                  cTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAt                                                                                  <  2:638036/100‑1 (MQ=255)
                  cTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAt                                                                                  <  1:2382915/100‑1 (MQ=255)
                  cTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAt                                                                                  <  2:4282764/100‑1 (MQ=255)
                  cTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAt                                                                                  <  2:194030/100‑1 (MQ=255)
                   tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATa                                                                                 <  2:3855353/100‑1 (MQ=255)
                   tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtc                                                                                 <  1:2239337/100‑2 (MQ=255)
                   tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtc                                                                                 <  1:4374367/100‑2 (MQ=255)
                   tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtc                                                                                 <  1:2938863/100‑2 (MQ=255)
                   tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtc                                                                                 <  2:462305/100‑2 (MQ=255)
                   tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtc                                                                                 <  2:2440768/100‑2 (MQ=255)
                   tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTATTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtc                                                                                 <  1:2714999/100‑2 (MQ=255)
                     tGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACg                                                                               <  2:1214650/100‑1 (MQ=255)
                      gCAAGGTCAAGGTGCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCGGCCATTAATGGCAGCCAtcg                                                                                <  1:1311368/98‑3 (MQ=255)
                      gCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGt                                                                              >  2:3846449/1‑100 (MQ=255)
                      gCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGt                                                                              >  2:1776324/1‑100 (MQ=255)
                       caaggtcaaggtCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTa                                                                             >  2:309748/1‑100 (MQ=255)
                       caaggtcaaggtCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTa                                                                             >  1:1367074/1‑100 (MQ=255)
                       caaggtcaaggtCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTa                                                                             >  2:2004401/1‑100 (MQ=255)
                       caaggtcaaggtCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtcgaaa                                                                             <  1:3504287/100‑6 (MQ=38)
                          ggtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACtt                                                                          >  2:2752412/1‑100 (MQ=255)
                          ggtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACtt                                                                          >  2:3507821/1‑100 (MQ=255)
                           gtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTCCTTc                                                                         >  1:115947/1‑100 (MQ=255)
                           gtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTc                                                                         <  1:1660464/100‑1 (MQ=255)
                             caaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCAt                                                                       >  2:1068494/1‑100 (MQ=255)
                               aggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTTTTTTTTTTTTATGGCTGCCATTAATGGCGGCCATACGTACTTCAt                                                                       >  1:2848841/1‑98 (MQ=255)
                               aggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGc                                                                     <  2:3922915/100‑1 (MQ=255)
                               aggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGc                                                                     >  2:3629777/1‑100 (MQ=255)
                               aggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGc                                                                     >  2:2778738/1‑100 (MQ=255)
                                ggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGca                                                                    <  2:3426296/100‑1 (MQ=255)
                                ggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGca                                                                    <  1:2437506/100‑1 (MQ=255)
                                 gtcCTTAGGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcag                                                                   <  1:208724/100‑1 (MQ=255)
                                   ccTTACGTCCTCGTCCTGCGGAAGCAAGGCCTTCAGCAATTTCTTTTTGACGTTCTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcag                                                                   <  1:1525565/98‑1 (MQ=255)
                                    cTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaac                                                                >  1:3909714/1‑100 (MQ=255)
                                     ttATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaaca                                                               <  1:847504/100‑1 (MQ=255)
                                      tATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaacag                                                              <  2:379300/100‑1 (MQ=255)
                                       aTGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaacaga                                                             <  2:1254606/100‑1 (MQ=255)
                                         gtgCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCagaacagaac                                                           <  2:13586/100‑1 (MQ=255)
                                          tgCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacg                                                          >  1:3473832/1‑100 (MQ=255)
                                           gCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGAAGAACAgaacga                                                         >  1:3541654/1‑100 (MQ=255)
                                            cTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacgaa                                                        >  2:1332158/1‑100 (MQ=255)
                                            cTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacgaa                                                        <  2:1516579/100‑1 (MQ=255)
                                            cTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacgaa                                                        >  1:541423/1‑100 (MQ=255)
                                             tCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacgaac                                                       >  2:1821897/1‑100 (MQ=255)
                                             tCGTTCTGCGGAAGCAAGGCCGTCAACAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacgaac                                                       <  1:3320315/100‑1 (MQ=255)
                                               gTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTTTTTTTTTTTTATGGCTGCCATTAGTGGCTGCCATATTTTCTTTATGTAGAACAgaacgaacgg                                                     >  1:2573865/1‑98 (MQ=255)
                                               gTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAg                                                     >  2:822441/1‑100 (MQ=255)
                                                 tCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGa                                                   >  1:2304151/1‑100 (MQ=255)
                                                 tCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGa                                                   <  1:774396/100‑1 (MQ=255)
                                                  cTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGAc                                                  <  1:1894490/100‑1 (MQ=255)
                                                   tGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACa                                                 >  2:1648351/1‑100 (MQ=255)
                                                   tGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACa                                                 >  2:1542093/1‑100 (MQ=255)
                                                   tGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACa                                                 >  2:4304665/1‑100 (MQ=255)
                                                       gAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAAcg                                             <  2:2262169/100‑1 (MQ=255)
                                                              ggCCGTCAGCAATTTCTTTTTGACGTTTTTTTATTTCTTCCATTAATGGCGCCCATACCTACTTCCTGCAGAGCAGAACGAACAGGACAAACGTGAtagc                                      >  1:3084596/1‑100 (MQ=255)
                                                              ggCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggc                                      >  1:2537634/1‑100 (MQ=255)
                                                                ccGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTGATGCACAACAGAACGAACAGGACAAACGCGAtggcct                                    >  1:2974299/1‑100 (MQ=255)
                                                                ccGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggc                                      <  1:1815104/98‑1 (MQ=255)
                                                                      gCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGa                              >  1:4346937/1‑100 (MQ=255)
                                                                       cAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAg                             <  1:3331167/100‑1 (MQ=255)
                                                                       cAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAg                             <  1:4246178/100‑1 (MQ=255)
                                                                        aaTTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAgg                            <  1:2000757/100‑1 (MQ=255)
                                                                           ttCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAtt                         <  2:755677/100‑1 (MQ=255)
                                                                           ttCTTTTTGACGTTTTTCGAGGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAtt                         >  1:2700699/1‑100 (MQ=255)
                                                                              ttttttttttttttCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACCAACGCGATAGTTTTGCCGAGGAttttt                      >  1:692061/10‑100 (MQ=255)
                                                                               ttttGACGTTTTTAGATGGCTGCCATTAATGGCGGACATACGTACTTCATGCAGCACAGAACGAACAGGACAAACGCGATGGCCCGGGCgggg                            >  1:4291052/1‑93 (MQ=255)
                                                                                   gACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGtt                 >  2:3262929/1‑100 (MQ=255)
                                                                                    aCGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTa                <  2:3974016/100‑1 (MQ=255)
                                                                                     cGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGGACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTaa               >  1:3292851/1‑100 (MQ=255)
                                                                                     cGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTaa               >  2:922985/1‑100 (MQ=255)
                                                                                     cGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTaa               >  2:1956177/1‑100 (MQ=255)
                                                                                     cGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTaa               >  2:1549046/1‑100 (MQ=255)
                                                                                     cGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTaa               <  2:1408239/100‑1 (MQ=255)
                                                                                       agtacgtaTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGa             >  2:3388922/8‑100 (MQ=38)
                                                                                        gtacgtaTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGa             >  1:586434/7‑99 (MQ=38)
                                                                                          ttCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTc          >  2:1251857/1‑100 (MQ=255)
                                                                                          acgtaTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACATGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTc          >  1:2485525/5‑100 (MQ=38)
                                                                                           ncGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTca         >  1:3793401/2‑100 (MQ=255)
                                                                                            cGATGGCTGACATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTcac        <  2:1762852/100‑1 (MQ=255)
                                                                                             taTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTcaca       >  2:3755695/2‑100 (MQ=255)
                                                                                             gATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTcaca       <  1:2143814/100‑1 (MQ=255)
                                                                                             gATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTcaca       <  1:4132078/100‑1 (MQ=255)
                                                                                               tGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGc     >  2:4176295/1‑100 (MQ=255)
                                                                                                 gCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGcac   >  2:3766921/1‑100 (MQ=255)
                                                                                                 gCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGcac   <  1:3875607/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
CTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACA  >  NC_000913/3920697‑3920894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: