Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,920,809 G→A 17.1% P27L (CCG→CTG)  atpF ← F0 sector of membrane‑bound ATP synthase, subunit b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,920,8090GA17.1% 485.3 / 76.9 204P27L (CCG→CTG) atpFF0 sector of membrane‑bound ATP synthase, subunit b
Reads supporting (aligned to +/- strand):  ref base G (86/82);  new base A (15/20);  total (102/102)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.76e-01

Only 100 of 218 total aligned reads displayed.

TGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAG  >  NC_000913/3920711‑3920908
                                                                                                  |                                                                                                   
 ggCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcggc                                                                                                   <  1:2665959/100‑1 (MQ=255)
 ggCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcggc                                                                                                   <  2:1499811/100‑1 (MQ=255)
  gCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGcc                                                                                                  <  1:1572574/100‑1 (MQ=255)
   ccTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCa                                                                                                 >  1:3715400/1‑100 (MQ=255)
    cTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAt                                                                                                <  2:194030/100‑1 (MQ=255)
    cTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAt                                                                                                <  2:4282764/100‑1 (MQ=255)
     tttGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATa                                                                                               <  2:3855353/100‑1 (MQ=255)
       tGCAAGGTCAAGGTCCTTGTGTGCTGGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtc                                                                                               <  1:612044/98‑2 (MQ=39)
       tGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACg                                                                                             <  2:2441489/100‑1 (MQ=255)
        gCAAGGTCAAGTTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGAGGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGt                                                                                            >  1:236213/1‑100 (MQ=255)
        gCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGt                                                                                            >  2:1281115/1‑100 (MQ=255)
        gCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGt                                                                                            >  2:3846449/1‑100 (MQ=255)
        gCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGt                                                                                            <  2:2149171/100‑1 (MQ=255)
        gCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGt                                                                                            >  1:4251067/1‑100 (MQ=255)
         caaggtcaaggtCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTa                                                                                           >  2:309748/1‑100 (MQ=255)
         caaggtcaaggtCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAtcgaaa                                                                                           <  1:3504287/100‑6 (MQ=38)
            ggtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACtt                                                                                        <  1:3400723/100‑1 (MQ=255)
            ggtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACtt                                                                                        >  1:1383758/1‑100 (MQ=255)
            ggtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACtt                                                                                        >  2:3507821/1‑100 (MQ=255)
             gtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTCCTTc                                                                                       >  1:115947/1‑100 (MQ=255)
             gtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTc                                                                                       <  1:1660464/100‑1 (MQ=255)
             gtcaaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTc                                                                                       >  2:1711076/1‑100 (MQ=255)
               caaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCAt                                                                                     >  2:1068494/1‑100 (MQ=255)
               caaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCAt                                                                                     <  2:2349004/100‑1 (MQ=255)
               caaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCAt                                                                                     >  1:379299/1‑100 (MQ=255)
                aaggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATg                                                                                    <  1:2421759/100‑1 (MQ=255)
                 aggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTTTTTTTTTTTTATGGCTGCCATTAATGGCGGCCATACGTACTTCAt                                                                                     >  1:2848841/1‑98 (MQ=255)
                 aggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGc                                                                                   >  2:3629777/1‑100 (MQ=255)
                 aggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGc                                                                                   <  1:2941906/100‑1 (MQ=255)
                 aggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGc                                                                                   <  2:827745/100‑1 (MQ=255)
                 aggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGc                                                                                   <  1:2253704/100‑1 (MQ=255)
                  ggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGca                                                                                  <  2:3426296/100‑1 (MQ=255)
                   gtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcag                                                                                 <  2:3715426/100‑1 (MQ=255)
                    tcCTTATGTGCTCGTTCTGCTGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcaga                                                                                <  2:4364033/100‑1 (MQ=255)
                      cTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaac                                                                              <  2:655862/100‑1 (MQ=255)
                      cTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaac                                                                              >  1:3909714/1‑100 (MQ=255)
                      cTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaac                                                                              >  2:273924/1‑100 (MQ=255)
                      cTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaac                                                                              <  1:270238/100‑1 (MQ=255)
                      cTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaa                                                                               >  1:584959/1‑99 (MQ=255)
                      cTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAAGGGCGGCCATACGTACTTCATGcagaac                                                                              <  1:2475940/100‑1 (MQ=255)
                       ttATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaaca                                                                             <  1:847504/100‑1 (MQ=255)
                        tATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaacag                                                                            <  2:379300/100‑1 (MQ=255)
                        tATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaacag                                                                            >  1:1629153/1‑100 (MQ=255)
                            tgCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacg                                                                        >  1:3473832/1‑100 (MQ=255)
                            tgCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacg                                                                        >  2:949930/1‑100 (MQ=255)
                             gCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGCTGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacga                                                                       >  2:1226848/1‑100 (MQ=255)
                             gCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacga                                                                       >  1:4160557/1‑100 (MQ=255)
                             gCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacga                                                                       >  2:1544421/1‑100 (MQ=255)
                             gCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGAAGAACAgaacga                                                                       >  1:3541654/1‑100 (MQ=255)
                              cTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacgaa                                                                      <  2:1516579/100‑1 (MQ=255)
                               tCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacgaac                                                                     <  2:1773281/100‑1 (MQ=255)
                                 gTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAg                                                                   >  1:1366196/1‑100 (MQ=255)
                                  ttCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAgn                                                                  <  2:565928/100‑2 (MQ=255)
                                   tCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGa                                                                 <  1:774396/100‑1 (MQ=255)
                                   tCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGa                                                                 <  1:3295235/100‑1 (MQ=255)
                                     tGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACa                                                               >  1:20981/1‑100 (MQ=255)
                                     tGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACa                                                               >  1:3321178/1‑100 (MQ=255)
                                      gCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACaa                                                              >  2:1356435/1‑100 (MQ=255)
                                         gAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAAcg                                                           <  2:1098509/100‑1 (MQ=255)
                                         gAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAAcg                                                           <  2:125440/100‑1 (MQ=255)
                                         gAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAAcg                                                           <  2:2262169/100‑1 (MQ=255)
                                           aGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAAcgcg                                                         >  2:1813498/1‑100 (MQ=255)
                                             cAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGCACTTCCTGCAGAACGGAACGAACAGGACAAACGCGAt                                                       >  1:2981088/1‑100 (MQ=255)
                                               aGGCCGTCAGCAATTTCTTTTTGACGTTTTGCGATGGCTGCCAGTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtgg                                                     >  1:4381224/1‑100 (MQ=255)
                                                ggCCGTCAGCAATTTCTTTTTGACGTTTTTTTATTTCTTCCATTAATGGCGCCCATACCTACTTCCTGCAGAGCAGAACGAACAGGACAAACGTGAtagc                                                    >  1:3084596/1‑100 (MQ=255)
                                                  ccGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggcc                                                   <  1:2584928/99‑1 (MQ=255)
                                                  ccGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggc                                                    <  1:1815104/98‑1 (MQ=255)
                                                        gCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGa                                            >  1:4346937/1‑100 (MQ=255)
                                                         cAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAg                                           <  1:2494381/100‑1 (MQ=255)
                                                          aaTTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAgg                                          <  1:2000757/100‑1 (MQ=255)
                                                          aaTTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAgg                                          <  2:4056605/100‑1 (MQ=255)
                                                           aTTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGa                                         >  2:4192618/1‑100 (MQ=255)
                                                            tttCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAt                                        >  2:2021490/1‑100 (MQ=255)
                                                             ttCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGAtt                                       <  2:755677/100‑1 (MQ=255)
                                                                 ttttGACGTTTTTAGATGGCTGCCATTAATGGCGGACATACGTACTTCATGCAGCACAGAACGAACAGGACAAACGCGATGGCCCGGGCgggg                                          >  1:4291052/1‑93 (MQ=255)
                                                                  tttGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGc                                  >  2:3314691/1‑100 (MQ=255)
                                                                   ttGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCg                                 <  2:1957136/100‑1 (MQ=255)
                                                                      aCGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTa                              <  2:3909744/100‑1 (MQ=255)
                                                                      aCGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTa                              <  2:3974016/100‑1 (MQ=255)
                                                                       cGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTaa                             <  2:1408239/100‑1 (MQ=255)
                                                                       cGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTaa                             >  2:922985/1‑100 (MQ=255)
                                                                         agtacgtaTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGa                           >  2:3388922/8‑100 (MQ=38)
                                                                          gtacgtaTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGAt                          >  2:1530661/7‑100 (MQ=38)
                                                                           nacgtaTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGAtt                         >  2:2773709/6‑100 (MQ=38)
                                                                            acgtatgaccgCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTc                        >  2:1890881/11‑100 (MQ=255)
                                                                            acgtaTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTc                        >  2:1365766/5‑100 (MQ=39)
                                                                              gtaTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTcac                      >  2:1582132/3‑100 (MQ=255)
                                                                              gtaTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTcac                      >  2:2832457/3‑100 (MQ=255)
                                                                               gATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTcaca                     <  1:2143814/100‑1 (MQ=255)
                                                                                 tGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGc                   >  1:2211047/1‑100 (MQ=255)
                                                                                   gCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGcac                 >  2:3766921/1‑100 (MQ=255)
                                                                                   gCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGcac                 <  1:3875607/100‑1 (MQ=255)
                                                                                      gCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAAt              >  1:2542421/1‑100 (MQ=255)
                                                                                      gCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAAt              >  1:3815689/1‑100 (MQ=255)
                                                                                         aTTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGcc           >  1:3181072/1‑100 (MQ=255)
                                                                                         aTTAATGGAGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGcc           <  1:2809631/100‑1 (MQ=255)
                                                                                             aTGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTa       <  1:3212628/100‑1 (MQ=255)
                                                                                               ggcggcCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTAtt     >  1:699961/1‑100 (MQ=255)
                                                                                               ggcggcCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTAtt     >  2:1838667/1‑100 (MQ=255)
                                                                                                  ggcCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACATTGCCTCTATTTAg  <  1:2730903/100‑1 (MQ=255)
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TGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAG  >  NC_000913/3920711‑3920908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: