Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,952,380 G→A 73.2% L60L (CTG→CTA ilvM → acetolactate synthase 2 small subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,952,3800GA73.2% 296.4 / 135.5 195L60L (CTG→CTAilvMacetolactate synthase 2 small subunit
Reads supporting (aligned to +/- strand):  ref base G (22/30);  new base A (72/69);  total (95/100)
Fisher's exact test for biased strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01

Only 100 of 195 total aligned reads displayed.

TTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAAT  >  NC_000913/3952284‑3952477
                                                                                                |                                                                                                 
tttCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTAttt                                                                                                <  1:2863953/100‑1 (MQ=255)
 ttCCACGTCTTCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGGCTTACTGTTTa                                                                                               <  1:3344898/100‑1 (MQ=255)
  tCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAg                                                                                              <  2:3388575/100‑1 (MQ=255)
  tCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAg                                                                                              <  1:2091202/100‑1 (MQ=255)
  tCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAg                                                                                              >  2:722070/1‑100 (MQ=255)
   ccACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAg                                                                                              <  1:4411972/99‑1 (MQ=255)
     aCGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCa                                                                                           >  1:1828048/1‑100 (MQ=255)
       gTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGt                                                                                         <  1:1077846/100‑1 (MQ=255)
       gTCTGATCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTCCCAGCCCACGGACGGTCGACTTACTATTTAGTCa                                                                                           >  1:2178277/1‑98 (MQ=255)
        tCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGtt                                                                                        >  1:147780/1‑100 (MQ=255)
        tCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGtt                                                                                        <  1:1946615/100‑1 (MQ=255)
           gCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTtaaa                                                                                     <  1:1507626/100‑1 (MQ=255)
           gCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTtaaa                                                                                     <  2:333710/100‑1 (MQ=255)
           gCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTtaaa                                                                                     >  2:3936005/1‑100 (MQ=255)
             tCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTtaaata                                                                                   <  2:3863156/100‑1 (MQ=255)
              cAATGAATATGGCCGCCGCCAGGGATGCACAAAAGATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGtt                                                                                        <  1:2798168/94‑1 (MQ=255)
              cAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTtaaataa                                                                                  >  1:2599889/1‑100 (MQ=255)
              cAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTtaaata                                                                                   >  1:996031/1‑99 (MQ=255)
              cAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAATATCGAATTGACCGTTGCCAGCCCACGGCCGGTCGACTTACTGTTTAGTCAGTtaaataaa                                                                                 >  1:916233/1‑100 (MQ=255)
               aaTGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTtaaataaa                                                                                 >  1:1795223/1‑100 (MQ=255)
                aTGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAAc                                                                                >  2:2192293/1‑100 (MQ=255)
                aTGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAAc                                                                                <  2:2599230/100‑1 (MQ=255)
                 tGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACt                                                                               >  2:2575748/1‑100 (MQ=255)
                 tGAATATGGCCGCCGACAGCGAAGCACAAAATATACATATCGAATTGCCCGTTGGCAGCCAACGGACAGCAGCCTGAATGTTAAGTCAGtt                                                                                        >  1:3495673/1‑91 (MQ=255)
                   aaTATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACtgg                                                                             <  2:2661781/100‑1 (MQ=255)
                    atatGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACtggt                                                                            <  2:618920/100‑1 (MQ=255)
                     tatGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACtggtg                                                                           <  2:2973475/100‑1 (MQ=255)
                      atGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACtggtgg                                                                          >  2:2255781/1‑100 (MQ=255)
                       tGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGa                                                                         >  1:1766453/1‑100 (MQ=255)
                       tGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACtggtgg                                                                          <  1:2027923/99‑1 (MQ=255)
                       tGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGa                                                                         >  1:483066/1‑100 (MQ=255)
                        ggCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGAc                                                                        <  2:3536398/100‑1 (MQ=255)
                        ggCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGAc                                                                        >  2:853018/1‑100 (MQ=255)
                         gccgccgccAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACg                                                                       >  2:276535/1‑100 (MQ=255)
                         gccgccgccAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACg                                                                       >  1:3430948/1‑100 (MQ=255)
                             ccgccAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGc                                                                   <  2:2506126/100‑1 (MQ=255)
                             ccgccAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGc                                                                   <  1:2542305/100‑1 (MQ=255)
                                  aGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACg                                                              >  1:627460/1‑100 (MQ=255)
                                    cGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCATGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGt                                                             >  2:1439788/1‑99 (MQ=255)
                                     gATGCACAAAATATAAATATCGAAGTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGtt                                                            <  2:3545531/99‑1 (MQ=255)
                                      aTGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGc                                                          <  2:3244061/100‑1 (MQ=255)
                                      aTGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGc                                                          <  2:809817/100‑1 (MQ=255)
                                      aTGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGc                                                          <  2:1893566/100‑1 (MQ=255)
                                      aTGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGc                                                          <  2:559945/100‑1 (MQ=255)
                                        gCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCa                                                        <  2:1327040/100‑1 (MQ=255)
                                         cacaAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCAt                                                       >  2:1525852/1‑100 (MQ=255)
                                           caAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCt                                                     >  2:2417254/1‑100 (MQ=255)
                                            aaaaTATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTg                                                    <  1:1440691/100‑1 (MQ=255)
                                            aaaaTATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTg                                                    >  1:4155542/1‑100 (MQ=255)
                                            aaaaTATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTg                                                    <  2:3057177/100‑1 (MQ=255)
                                             aaatataaatatCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGc                                                   <  2:3917365/100‑1 (MQ=255)
                                               ntataaatatCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCa                                                 >  2:4151865/2‑100 (MQ=255)
                                                  taaatatCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCagag                                              >  1:38747/1‑100 (MQ=255)
                                                  taaatatCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCagag                                              <  2:3565309/100‑1 (MQ=255)
                                                  taaatatCGAATTGACCATTGCCAGCGCACGGTCGGTCGACTTACTGTTGAGTCGTTTAAAGAAACTGGTGGAGGTTGCAGACGTTTCCATCtaaaagag                                              >  1:2629418/1‑93 (MQ=255)
                                                   aaatatCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCacag                                              >  1:2055271/1‑99 (MQ=255)
                                                   aaatatCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGc                                             >  2:4360823/1‑100 (MQ=255)
                                                    aatatCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGca                                            <  1:2310417/100‑1 (MQ=255)
                                                    aatatCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGca                                            >  1:361394/1‑100 (MQ=255)
                                                    aatatCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCGTCTGCCAGAGca                                            <  1:3935975/100‑1 (MQ=255)
                                                    aatatCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGca                                            <  2:1131811/100‑1 (MQ=255)
                                                     atatCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGcac                                           <  1:4303967/100‑1 (MQ=255)
                                                      tatCTAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGc                                             <  1:4189610/97‑1 (MQ=255)
                                                      tatCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGcaca                                          <  2:361395/100‑1 (MQ=255)
                                                        tCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACaa                                         >  2:895700/1‑99 (MQ=255)
                                                         cGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAAcc                                       >  2:1631814/1‑100 (MQ=255)
                                                         cGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAAcc                                       <  1:3212603/100‑1 (MQ=255)
                                                          gAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACca                                      <  1:67730/100‑1 (MQ=255)
                                                          gAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACca                                      >  2:1964819/1‑100 (MQ=255)
                                                          gAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACca                                      >  2:3794925/1‑100 (MQ=255)
                                                            aTTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACcaca                                    >  2:1183962/1‑100 (MQ=255)
                                                             ttGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACAt                                   >  2:3806006/1‑100 (MQ=255)
                                                              tccgatctGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACAtn                                  <  1:2144766/93‑2 (MQ=255)
                                                              tGACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATc                                  >  2:2462343/1‑100 (MQ=255)
                                                               gACCGTTGCCAGCCCTCGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATca                                 <  2:4340884/100‑1 (MQ=255)
                                                               gACCGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATca                                 <  1:3814204/100‑1 (MQ=255)
                                                                  cGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCacaa                              <  1:18973/100‑1 (MQ=255)
                                                                  cGTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCacaa                              <  1:1046859/100‑1 (MQ=255)
                                                                   gTTGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCacaac                             <  1:1295988/100‑1 (MQ=255)
                                                                    ttGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCacaaca                            <  2:2107969/100‑1 (MQ=255)
                                                                     tGCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCAcaacaa                           >  2:1396663/1‑100 (MQ=255)
                                                                     tGCCAGCCCACCGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCTTACACTTTGCCATCTGCCAGAGCACAACCACATCacaaca                            <  2:1261003/99‑1 (MQ=255)
                                                                      nccAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATcagatcgga                          >  2:2144773/2‑93 (MQ=255)
                                                                      gCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACaaa                          <  1:328506/100‑1 (MQ=255)
                                                                      gCCAGCCCACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACaaa                          >  1:3727734/1‑100 (MQ=255)
                                                                       ccAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAAt                         >  2:502729/1‑100 (MQ=255)
                                                                       ccAGCCCACGGTAGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAAt                         >  2:2899500/1‑100 (MQ=255)
                                                                        cAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATc                        <  1:2228130/100‑1 (MQ=255)
                                                                           cccACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCcgc                     >  1:3148038/1‑100 (MQ=255)
                                                                            ccACGGTCGGTCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCcgcg                    <  1:2373447/100‑1 (MQ=255)
                                                                              aCGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGcc                  <  2:424401/100‑1 (MQ=255)
                                                                               cggtcggtCGACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCt                 >  2:2333441/1‑100 (MQ=255)
                                                                                    ggtcgACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAgcg            <  2:2907219/100‑1 (MQ=255)
                                                                                     gtcgACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCATAGCACAACCACATCACAACAAATCCGCGCCTGAgcg            <  1:1017785/99‑1 (MQ=255)
                                                                                     gtcgACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAgcgc           >  1:3031911/1‑100 (MQ=255)
                                                                                      tcgACTTACTGTTTAGTCAGTTAAATATACTGGTGGACGCCGCACACGTTGCCATCCGCCAGAGCACAGCCACATCACAACATATCCCCGCCTGAgcgc           <  1:705554/99‑1 (MQ=255)
                                                                                      tcgACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAgcg            >  1:2378268/1‑98 (MQ=255)
                                                                                      tcgACTTACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAGCGCa          <  2:3428448/100‑1 (MQ=255)
                                                                                            tACTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAGCGCAAAAGGa    >  2:288812/1‑100 (MQ=255)
                                                                                             aCTATTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAGCGCAAAAGGaa   <  1:1261704/100‑1 (MQ=255)
                                                                                                |                                                                                                 
TTTCCACGTCTGCTCAATGAATATGGCCGCCGCCAGCGATGCACAAAATATAAATATCGAATTGACCGTTGCCAGCCCACGGTCGGTCGACTTACTGTTTAGTCAGTTAAATAAACTGGTGGACGTCGCACACGTTGCCATCTGCCAGAGCACAACCACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAAT  >  NC_000913/3952284‑3952477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: