Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,115,214 C→T 30.2% K122K (AAG→AAA glpX ← fructose 1,6‑bisphosphatase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,115,2140CT30.2% 238.0 / 109.1 158K122K (AAG→AAAglpXfructose 1,6‑bisphosphatase II
Reads supporting (aligned to +/- strand):  ref base C (52/57);  new base T (29/18);  total (81/77)
Fisher's exact test for biased strand distribution p-value = 1.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

CTCAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATGCGC  >  NC_000913/4115116‑4115310
                                                                                                  |                                                                                                
cTCAATGGTTTGCCGAGCGCCCCCGCTACATTGCGCATGTTATCCGCCAGCGTCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACCATCAGc                                                                                                  <  1:2898005/99‑1 (MQ=255)
cTCAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCt                                                                                                 <  1:3763558/100‑1 (MQ=255)
 tCAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCtt                                                                                                >  1:1228472/1‑100 (MQ=255)
  cAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTtc                                                                                               >  2:3795722/1‑100 (MQ=255)
     cGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTcc                                                                                            <  1:1563028/100‑1 (MQ=255)
       gTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCat                                                                                          >  2:3231148/1‑100 (MQ=255)
       gTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCat                                                                                          >  2:2013009/1‑100 (MQ=255)
        tttGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCata                                                                                         <  2:2085815/100‑1 (MQ=255)
         ttGCCGCGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCatat                                                                                        <  1:3321962/100‑1 (MQ=255)
           gCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGACAGGTTCAGATCAATGGTGGCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATAc                                                                                      >  1:2403263/1‑100 (MQ=255)
                gcgcCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATc                                                                                 >  1:3895425/1‑100 (MQ=255)
                gcgcCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATc                                                                                 >  1:1962947/1‑100 (MQ=255)
                  gccgccgcTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCg                                                                               >  2:1119393/1‑100 (MQ=255)
                   ccgccgcTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTATCCATATACATCTCCg                                                                               >  1:180878/1‑99 (MQ=255)
                    cgccgcTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGgc                                                                             <  2:2615237/100‑1 (MQ=255)
                      ccgcTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGgcgc                                                                           >  1:679998/1‑100 (MQ=255)
                       cgcTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCa                                                                          >  2:3654886/1‑100 (MQ=255)
                          tACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTg                                                                       <  1:492092/100‑1 (MQ=255)
                           aCATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGa                                                                      >  2:1792474/1‑100 (MQ=255)
                           aCATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGa                                                                      <  2:890655/100‑1 (MQ=255)
                            cATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAg                                                                     <  1:517396/100‑1 (MQ=255)
                            cATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAg                                                                     <  2:3590116/100‑1 (MQ=255)
                             aTTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAgg                                                                    <  2:1962954/100‑1 (MQ=255)
                               tGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGacg                                                                    >  2:3931649/1‑96 (MQ=255)
                                 cgcAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAagc                                                                >  2:4008661/1‑100 (MQ=255)
                                  gcAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAagca                                                               >  1:462811/1‑100 (MQ=255)
                                  gcAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAagca                                                               <  2:1228476/100‑1 (MQ=255)
                                   cAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAagcag                                                              <  2:2650324/100‑1 (MQ=255)
                                       ttATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCtt                                                          <  2:253903/100‑1 (MQ=255)
                                       ttATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCtt                                                          >  2:1523869/1‑100 (MQ=255)
                                         aTCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGCAGCAGCCttt                                                         >  1:2522047/1‑99 (MQ=255)
                                          tCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTAt                                                       >  2:2555094/1‑100 (MQ=255)
                                           ccGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATc                                                      <  2:2298016/100‑1 (MQ=255)
                                           ccGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATc                                                      >  1:1770672/1‑100 (MQ=255)
                                            cGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCg                                                     >  1:2252747/1‑100 (MQ=255)
                                            cGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCg                                                     <  1:744937/100‑1 (MQ=255)
                                            cGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCg                                                     >  1:809919/1‑100 (MQ=255)
                                            cGCCAGCGGCAGGTTCAGATCAATGGTGCCTTAGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGAATTGAGGAAGCAGCCTTTATCg                                                     >  2:4182433/1‑100 (MQ=255)
                                             nccAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGc                                                    >  2:3556665/2‑100 (MQ=255)
                                             gCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAAACAGTTTCTCCATATACATATCCCTCGCCGTGAGTAAGCACGCTTTATCGc                                                    >  1:2421822/1‑100 (MQ=255)
                                              ccAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGcc                                                   <  2:4183452/100‑1 (MQ=255)
                                              ccAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGcc                                                   <  2:1015127/100‑1 (MQ=255)
                                               cAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCt                                                  <  2:4180804/100‑1 (MQ=255)
                                               cAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCt                                                  <  2:4011431/100‑1 (MQ=255)
                                                aGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTa                                                 <  1:2095019/100‑1 (MQ=255)
                                                 gCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTAc                                                >  1:1119301/1‑100 (MQ=255)
                                                   ggCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTg                                              >  1:1324992/1‑100 (MQ=255)
                                                   ggCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTg                                              <  1:2719092/100‑1 (MQ=255)
                                                   ggCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTg                                              <  2:3644756/100‑1 (MQ=255)
                                                    gCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGc                                             <  2:531624/100‑1 (MQ=255)
                                                    gCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGc                                             <  1:2559530/100‑1 (MQ=255)
                                                     cAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGcc                                            <  1:2390820/100‑1 (MQ=255)
                                                      aGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCa                                           <  1:4079847/100‑1 (MQ=255)
                                                      aGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCa                                           <  2:266549/100‑1 (MQ=255)
                                                       ggTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAg                                          <  1:930500/100‑1 (MQ=255)
                                                       ggTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAg                                          >  2:4330280/1‑100 (MQ=255)
                                                        gTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGc                                         >  2:1084736/1‑100 (MQ=255)
                                                         ttCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCa                                        >  1:792260/1‑100 (MQ=255)
                                                            aGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCg                                     >  2:2167162/1‑100 (MQ=255)
                                                            aGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCg                                     >  1:650233/1‑100 (MQ=255)
                                                            aGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCg                                     >  2:1388411/1‑100 (MQ=255)
                                                             gATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCTCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGc                                    >  2:4350877/1‑100 (MQ=255)
                                                             gATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGc                                    >  1:3286384/1‑100 (MQ=255)
                                                              aTCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGcc                                   <  2:3478835/100‑1 (MQ=255)
                                                               tCAATTGTGCCTTTGGCTCCCGGCGCGACAATGTGGTTCTCCATATACATATCAGGCGCATTGAGGTAGCAGCCTTTTTAGCCTACGGCGAGCACCg                                     <  1:1338060/97‑1 (MQ=255)
                                                                 aaTGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAgc                                >  2:1761211/1‑100 (MQ=255)
                                                                  gatctGCCTTTGGCTCCAGGCCAGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAgcg                               <  1:883900/96‑1 (MQ=255)
                                                                  aTGGTGCCTTTGGCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAgcg                               >  2:2224545/1‑100 (MQ=255)
                                                                    ggTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGcgcg                             >  1:4369988/1‑100 (MQ=255)
                                                                      tGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGtt                           <  2:3879463/100‑1 (MQ=255)
                                                                       gCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGtt                           >  2:3340235/1‑99 (MQ=255)
                                                                       gCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTa                          <  1:1130791/100‑1 (MQ=255)
                                                                       cttccgatcTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTa                          <  1:3228417/92‑1 (MQ=255)
                                                                         cTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGc                        >  2:277562/1‑100 (MQ=255)
                                                                          tttGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGcc                       <  1:2792596/100‑1 (MQ=255)
                                                                             ggCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTgg                    >  1:3835018/1‑100 (MQ=255)
                                                                             ggCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTgg                    >  2:710345/1‑100 (MQ=255)
                                                                              gCTCCCGGCCTGACAATCACCTTCTCCATATACATATCCGGCGCATTAAGGAAGCAGCCTTTATCCTCTACTGGCACCACCGCCAGCTCGTTTGCCTg                     >  1:3676967/1‑98 (MQ=255)
                                                                              gCTCCCGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGc                   <  2:832614/100‑1 (MQ=255)
                                                                              gCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGc                   <  1:3562662/100‑1 (MQ=255)
                                                                              gCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGc                   <  2:2999235/100‑1 (MQ=255)
                                                                               cTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGcc                  >  2:3686928/1‑100 (MQ=255)
                                                                               cTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGcc                  <  1:277561/100‑1 (MQ=255)
                                                                                tCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGccc                 >  2:3319318/1‑100 (MQ=255)
                                                                                 cccGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGcccc                 >  1:1227041/1‑99 (MQ=255)
                                                                                 cccGGCCCGACAATCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCa                >  2:1944206/1‑100 (MQ=255)
                                                                                 cccGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTaagatcggaag                >  2:3228435/1‑90 (MQ=255)
                                                                                 cccGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCa                >  2:3167137/1‑100 (MQ=255)
                                                                                 cccGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCa                >  1:484988/1‑100 (MQ=255)
                                                                                  ccGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCAt               <  2:2706630/100‑1 (MQ=255)
                                                                                    ggCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCg             <  2:4251734/100‑1 (MQ=255)
                                                                                    ggCCCGAAAATCAGCTTCTCCATATACGTATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCg             <  2:62608/100‑1 (MQ=255)
                                                                                       ccGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCg          >  1:2879274/1‑100 (MQ=255)
                                                                                       ccGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCg          >  1:2869308/1‑100 (MQ=255)
                                                                                         gACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTc        <  2:3810431/100‑1 (MQ=255)
                                                                                           cAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCAt      <  1:3167119/100‑1 (MQ=255)
                                                                                            aaTCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATg     <  2:2742485/100‑1 (MQ=255)
                                                                                             aTCAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATgc    >  1:59675/1‑100 (MQ=255)
                                                                                              tCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATgcg   >  2:206054/1‑100 (MQ=255)
                                                                                               cAGTTTCTCCATATACATATCCGGCGCATTGAGGAAGCCGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATgcgc  >  1:2728664/1‑100 (MQ=255)
                                                                                                  |                                                                                                
CTCAACGGTTTGCCGAGCGCCGCCGCTACATTGCGCAGGTTATCCGCCAGCGGCAGGTTCAGATCAATGGTGCCTTTGGCTCCCGGCCCGACAATCAGCTTCTCCATATACATATCCGGCGCATTGAGGAAGCAGCCTTTATCGCCTACTGCCAGCACCGCCAGCGCGTTAGCCTGGCCCATCGCCGTCATGCGC  >  NC_000913/4115116‑4115310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: