Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,177,171 T→A 17.9% intergenic (+43/‑187) tufB → / → secE translation elongation factor EF‑Tu 2/preprotein translocase membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,177,1710TA17.9% 391.9 / 76.9 168intergenic (+43/‑187)tufB/secEtranslation elongation factor EF‑Tu 2/preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (60/76);  new base A (12/18);  total (73/95)
Fisher's exact test for biased strand distribution p-value = 8.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

Only 100 of 180 total aligned reads displayed.

CCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATA  >  NC_000913/4177074‑4177269
                                                                                                 |                                                                                                  
ccGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAttt                                                                                                  <  1:1675351/100‑1 (MQ=255)
   tGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACAATTGACGCAATGCGCACTAAAAGGGCATCATTTGAt                                                                                               >  2:663492/1‑100 (MQ=255)
     aaGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGc                                                                                             <  2:1003361/100‑1 (MQ=255)
       ggcggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGccc                                                                                           >  2:1705418/1‑100 (MQ=255)
       ggcggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGccc                                                                                           >  1:2132430/1‑100 (MQ=255)
         cggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtt                                                                                         <  1:2864074/100‑1 (MQ=255)
         cggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtt                                                                                         <  1:1422828/100‑1 (MQ=255)
         cggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtt                                                                                         <  1:1492292/100‑1 (MQ=255)
          ggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttt                                                                                        <  1:2953870/100‑1 (MQ=255)
          gaccGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttt                                                                                        <  1:2683438/98‑1 (MQ=255)
           gcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGATCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtttt                                                                                       <  1:1076044/100‑1 (MQ=255)
           gcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtttt                                                                                       <  2:3973235/100‑1 (MQ=255)
            ccGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtttta                                                                                      <  1:2545612/100‑2 (MQ=255)
             cGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTg                                                                                     <  2:409641/100‑1 (MQ=255)
             cGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttttag                                                                                     <  2:122326/100‑3 (MQ=255)
             cGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttttag                                                                                     <  2:919299/100‑3 (MQ=255)
             cGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttttag                                                                                     <  2:829464/100‑3 (MQ=255)
              gTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGc                                                                                    >  2:3342347/1‑100 (MQ=255)
              gTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttttagt                                                                                    <  1:3751730/100‑4 (MQ=255)
              gTACCGTTGACGGGGGCGTTGTAGCAAAAGTTCTGAGCTGATTGCCGAGAACGTTAGACGCAATGCGCACTAAAGGGGCATCAAATGGTGCCCttttagt                                                                                    <  2:4392897/100‑4 (MQ=255)
               tACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCa                                                                                   >  2:852412/1‑100 (MQ=255)
                aCCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCAc                                                                                  <  2:3353937/100‑1 (MQ=255)
                aCCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCAc                                                                                  >  2:716841/1‑100 (MQ=255)
                aCCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCAc                                                                                  <  2:297234/100‑1 (MQ=255)
                 ccGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACg                                                                                 <  1:4162102/100‑1 (MQ=255)
                      ggCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTc                                                                            <  2:4123437/100‑1 (MQ=255)
                           gggCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTAcc                                                                       <  1:3439055/100‑1 (MQ=255)
                           gggCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTAcc                                                                       <  2:4100131/100‑1 (MQ=255)
                             gCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCTCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCa                                                                      <  2:2778/99‑1 (MQ=255)
                               gTTGTAGTAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTAcc                                                                       <  2:1128428/96‑1 (MQ=255)
                                 tGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAAcc                                                                 <  1:820438/100‑1 (MQ=255)
                                 tGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAAcc                                                                 <  2:4228469/100‑1 (MQ=255)
                                 tGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTAACAGAAcc                                                                 >  1:795595/1‑100 (MQ=255)
                                    aGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTgg                                                              <  2:3590452/100‑1 (MQ=255)
                                     gCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGc                                                             <  1:761876/100‑1 (MQ=255)
                                     gCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGc                                                             >  1:4302056/1‑100 (MQ=255)
                                      cAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGTACCTGCCt                                                            >  1:4068766/1‑100 (MQ=255)
                                       aaaaGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCtc                                                           <  1:555297/100‑1 (MQ=255)
                                       aaaaGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCtc                                                           <  2:1190491/100‑1 (MQ=255)
                                        aaaGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACCAAAAGGGCATCATTTGATGCCCTTTTTGCACGCGTTCGTACCAGAACCTGGCtca                                                          <  1:203982/100‑1 (MQ=255)
                                           gTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCtcatca                                                       >  1:1867872/1‑100 (MQ=255)
                                            ttCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAg                                                      <  1:702957/100‑1 (MQ=255)
                                            ttCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAATTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCtcttca                                                       >  1:2840845/1‑99 (MQ=255)
                                              cTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTg                                                    <  2:755528/100‑1 (MQ=255)
                                                gAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGAt                                                  >  2:4268392/1‑100 (MQ=255)
                                                 aGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGAtt                                                 >  2:4031249/1‑100 (MQ=255)
                                                       ttGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCttt                                           <  2:3305945/100‑1 (MQ=255)
                                                        tGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTg                                          <  2:1997839/100‑1 (MQ=255)
                                                        tGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTg                                          <  2:2669627/100‑1 (MQ=255)
                                                        tGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTg                                          <  1:2455261/100‑1 (MQ=255)
                                                         gCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTg                                          >  1:2505416/1‑99 (MQ=255)
                                                         gCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGt                                         >  2:1753624/1‑100 (MQ=255)
                                                         gCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGt                                         >  1:1083751/1‑100 (MQ=255)
                                                          gatctAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTc                                        <  2:98506/96‑1 (MQ=255)
                                                          ccGATAACATTTGACGCAATGCGCACTAAAATGGCATCATTTGATGCCCTTTTTGCACGCTGTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTc                                        <  1:2717591/100‑1 (MQ=255)
                                                            gATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCa                                       <  1:3621983/99‑1 (MQ=255)
                                                            gATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCAt                                      >  2:2431600/1‑100 (MQ=255)
                                                            gATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCAt                                      <  2:2316345/100‑1 (MQ=255)
                                                             aTAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATa                                     >  1:1873195/1‑100 (MQ=255)
                                                              tAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATaa                                    <  2:1875205/100‑1 (MQ=255)
                                                                  aTTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCAt                                <  2:297997/100‑1 (MQ=255)
                                                                  aTTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCAt                                >  2:2207498/1‑100 (MQ=255)
                                                                    ttGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTg                              <  1:3681572/100‑1 (MQ=255)
                                                                     tGACGCAATGTGCACTAAAAGGGCACCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGc                             <  1:3343175/100‑1 (MQ=255)
                                                                     tGACGCAATGCGCACTAAAAGGGCATCCTTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGc                             >  1:2118729/1‑100 (MQ=255)
                                                                      gACGGAATGCGCACTAAAAGGGCATCATTTGATGTCCTTTTTGCACGCTTTCGTACCAGGACCTGGCTCATGTGTGATTTTCTTTGTCATAATCAtcggt                            >  1:2737457/1‑96 (MQ=255)
                                                                      gACGCAATGCGCACTAAAAGGGCATCGTTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGc                             <  1:2067724/99‑1 (MQ=255)
                                                                      gACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCt                            <  2:4208530/100‑1 (MQ=255)
                                                                      gACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCt                            <  1:2399484/100‑1 (MQ=255)
                                                                       aCGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTg                           <  2:3513085/100‑1 (MQ=255)
                                                                        cGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTga                          <  1:234743/100‑1 (MQ=255)
                                                                        cGCAATGCGCACTAAAAGGGCATCATTCGATGCCCTTATTGCACGCTTTCGTACCAGAACCTGGCTCATTAGTGATTCTCTTTGTCATAAACATTGCTg                           >  1:1790220/1‑99 (MQ=255)
                                                                          cAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTgaga                        <  2:1947536/100‑1 (MQ=255)
                                                                           aaTGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGAc                       >  1:3709686/1‑100 (MQ=255)
                                                                            aTGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACa                      <  2:2101885/100‑1 (MQ=255)
                                                                             agcgtgcaaaaaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAg                     >  1:3204769/9‑100 (MQ=37)
                                                                              gcgcACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACTAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAgg                    >  1:4369717/1‑100 (MQ=255)
                                                                              gcgcACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAgg                    <  1:2207488/100‑1 (MQ=255)
                                                                               cgtgcaaaaaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGc                   >  1:1710530/7‑100 (MQ=38)
                                                                               cgtgcaaaaaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGc                   >  1:37416/7‑100 (MQ=38)
                                                                                  gcaaaaaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGctct                >  1:1177740/4‑100 (MQ=255)
                                                                                  aCTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGctct                >  1:3449382/1‑100 (MQ=255)
                                                                                  aCTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGctct                >  1:2139620/1‑100 (MQ=255)
                                                                                   caaaaaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTg               >  2:2482092/3‑100 (MQ=255)
                                                                                   cTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTg               >  2:1737854/1‑100 (MQ=255)
                                                                                   cTAAAAGGGCACCATTTGATGTCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTg               >  2:2877623/1‑100 (MQ=255)
                                                                                    tAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGt              <  2:1728332/100‑1 (MQ=255)
                                                                                     aaaaGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATATTCTTTGTCATAATCATTGCTGAGACAGGCTCTGtt             >  1:3158960/1‑100 (MQ=255)
                                                                                     aaaaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTGGTCATAATCATTGCTGAGACAAGCTCTGtt             >  1:1531609/1‑100 (MQ=39)
                                                                                      aaaGGGCATTATTTGGTGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTg            <  1:3962718/100‑1 (MQ=255)
                                                                                       aaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGa           >  2:1346232/1‑100 (MQ=255)
                                                                                       aaGGGCATCAAATGATGCCCTTTTAGTGCGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGa           >  2:823822/1‑100 (MQ=38)
                                                                                        aGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAg          <  2:2434554/100‑1 (MQ=255)
                                                                                          ggCATCATTTGATGCCCTTTTTTTTTTTTTTTTTAACAGAAACTGGCTCATCAGTGATTTTTTTTGTCTTAATTATTGCTGAGTCAGTTTCTGTTTAgg         >  1:455782/1‑99 (MQ=255)
                                                                                          ggCATCATTTGATGCCCTTTTTTTATTTTTTTGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAggg        >  1:2279670/1‑100 (MQ=255)
                                                                                          ggCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAggg        <  2:214392/100‑1 (MQ=255)
                                                                                             atcatTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGt     <  1:1398550/100‑1 (MQ=255)
                                                                                               catTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGtat   <  2:3715814/100‑1 (MQ=255)
                                                                                               catTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGtat   <  1:337491/100‑1 (MQ=255)
                                                                                                atTTGATGCCCTTGTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTGGTCATAGTCATCGCTGAGACAGGCTCTGTTGAGGGCGta    >  1:3512381/1‑98 (MQ=255)
                                                                                                 |                                                                                                  
CCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATA  >  NC_000913/4177074‑4177269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: