Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,223,854 T→C 17.4% R9R (CGT→CGC metH → homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,223,8540TC17.4% 352.5 / 66.8 161R9R (CGT→CGCmetHhomocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
Reads supporting (aligned to +/- strand):  ref base T (61/72);  new base C (15/13);  total (76/85)
Fisher's exact test for biased strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

Only 100 of 174 total aligned reads displayed.

CACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTT  >  NC_000913/4223756‑4223953
                                                                                                  |                                                                                                   
cacaGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgcgtg                                                                                                    <  2:3956291/100‑1 (MQ=255)
cacaGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgcgtg                                                                                                    <  2:350721/100‑1 (MQ=255)
    gATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCa                                                                                                <  2:1822533/100‑1 (MQ=255)
     aTGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAg                                                                                               <  2:1732763/100‑1 (MQ=255)
     aTGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAg                                                                                               <  2:340853/100‑1 (MQ=255)
       gCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGtt                                                                                             >  2:2562291/1‑100 (MQ=255)
        cGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTa                                                                                            <  2:1423486/100‑1 (MQ=255)
        cGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTa                                                                                            <  1:2433746/100‑1 (MQ=255)
         gTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGCACGCAGttgt                                                                                           <  1:926955/100‑3 (MQ=255)
         gTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCATGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTaa                                                                                           >  1:2228360/1‑100 (MQ=255)
          tttATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTaaa                                                                                          >  2:4225361/1‑100 (MQ=255)
           ttATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGCACGCAGttgttc                                                                                         <  2:4089496/100‑5 (MQ=255)
           ttATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGCACGCAGttgttc                                                                                         <  2:1304190/100‑5 (MQ=255)
            tATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGTGTTGAGCGTATCTGGAGCAAGTATGAGAAGCAAAGTGGAACAACTGCGTGCGCAGTTAAAt                                                                                         >  1:261267/1‑99 (MQ=255)
            tATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGCACGCAGttgttcc                                                                                        <  2:1391851/100‑6 (MQ=255)
             aTGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGCACGCAGttgttcca                                                                                       <  2:4170257/100‑7 (MQ=255)
              tGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGaa                                                                                      >  2:3625623/1‑100 (MQ=255)
               gCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAAc                                                                                     >  2:3667102/1‑100 (MQ=255)
                ccAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACg                                                                                    <  1:1863196/100‑1 (MQ=255)
                ccAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACg                                                                                    <  2:3386382/100‑1 (MQ=255)
                ccAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACg                                                                                    >  2:3288286/1‑100 (MQ=255)
                ccAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACg                                                                                    >  2:4383392/1‑100 (MQ=255)
                ccAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGCACGCAGttgttccac                                                                                      <  2:497838/98‑8 (MQ=255)
                 cAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGt                                                                                   <  2:2944864/100‑1 (MQ=255)
                       ggTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTg                                                                             >  2:1160448/1‑100 (MQ=255)
                        gTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTgg                                                                            <  2:2964041/100‑1 (MQ=255)
                        gTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTgg                                                                            <  2:1122322/100‑1 (MQ=255)
                        gTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTgg                                                                            <  2:905806/100‑1 (MQ=255)
                          ttgttgAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTg                                                                          <  1:2948035/100‑1 (MQ=255)
                           tgttgAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGc                                                                         <  1:3319052/100‑1 (MQ=255)
                            gttgAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCt                                                                        <  2:2734380/100‑1 (MQ=255)
                              tgAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTg                                                                       >  1:768147/1‑99 (MQ=255)
                              tgAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAAAGTATTCTGGTGCTgg                                                                      >  1:786734/1‑100 (MQ=255)
                               gAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGa                                                                     >  1:2449801/1‑100 (MQ=255)
                                 aTTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGGTAAATGAACGTATTCTGGTGCTGGAc                                                                    >  1:3953605/1‑99 (MQ=255)
                                    tttATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGAcggcg                                                                >  1:536525/1‑100 (MQ=255)
                                     ttattaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGAcggcgg                                                               >  2:3901272/1‑100 (MQ=255)
                                     ttattaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGAcggcgg                                                               <  1:1160444/100‑1 (MQ=255)
                                      tattaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGAGCAGTTAAATGAACGTATTCTGGTACTGGAcggcg                                                                >  1:2716863/1‑98 (MQ=255)
                                         taAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATg                                                           <  2:3234254/100‑1 (MQ=255)
                                         taAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATg                                                           >  1:3637204/1‑100 (MQ=255)
                                         taAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATg                                                           <  2:2457828/100‑1 (MQ=255)
                                          aaaTCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATgg                                                          >  1:618864/1‑100 (MQ=255)
                                          aaaTCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATg                                                           >  1:2858586/1‑99 (MQ=255)
                                            aTCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGc                                                        >  2:423322/1‑100 (MQ=255)
                                            aTCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGc                                                        <  1:291226/100‑1 (MQ=255)
                                             tCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCa                                                       >  1:3807101/1‑100 (MQ=255)
                                             tCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCa                                                       <  2:110700/100‑1 (MQ=255)
                                              cTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCAc                                                      >  2:277124/1‑100 (MQ=255)
                                               tGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCAcc                                                     <  1:1014397/100‑1 (MQ=255)
                                                 ggTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCAt                                                   >  2:1469305/1‑100 (MQ=255)
                                                  gTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCAt                                                   >  1:1489477/1‑99 (MQ=255)
                                                    tGAGCGTGTCGGGAGCAACTGTGAGCAGAAAAGCGGAACAACTGCGTGCGCAGCTACATGAACGTATTCTGGTGCTGGACGGCGGTATGGTCACCATg                                                  >  1:1329205/1‑98 (MQ=255)
                                                     gAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATc                                               <  2:2132105/100‑1 (MQ=255)
                                                      aGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGCGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGAGCTGGACGGCGGTATGGGCACCATGATcc                                              >  1:99451/1‑100 (MQ=255)
                                                          tgtCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCagag                                          <  2:2720242/100‑1 (MQ=255)
                                                          tgtCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCagag                                          <  2:1604121/100‑1 (MQ=255)
                                                          tgtCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCagag                                          <  2:2591951/100‑1 (MQ=255)
                                                             cGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTa                                       >  2:2736714/1‑100 (MQ=255)
                                                              gggAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTAt                                      >  2:2416936/1‑100 (MQ=255)
                                                              gggAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTAt                                      >  1:2697525/1‑100 (MQ=255)
                                                               ggAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTAt                                      <  1:2384106/99‑1 (MQ=255)
                                                               ggAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATc                                     <  1:1630563/100‑1 (MQ=255)
                                                               ggAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATc                                     <  1:1521231/100‑1 (MQ=255)
                                                                gAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCg                                    <  2:3297774/100‑1 (MQ=255)
                                                                gAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCg                                    <  2:3777266/100‑1 (MQ=255)
                                                                gAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCg                                    <  1:2500299/100‑1 (MQ=255)
                                                                     aGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGa                               >  2:4139679/1‑100 (MQ=255)
                                                                      gtgtGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGaa                              >  1:4183662/1‑100 (MQ=255)
                                                                        gtgAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATAGACTGAACg                            >  1:3452547/1‑100 (MQ=255)
                                                                         tgAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGa                           >  2:297258/1‑100 (MQ=255)
                                                                         tgAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGa                           <  1:2373630/100‑1 (MQ=255)
                                                                         tgAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGa                           <  2:1568926/100‑1 (MQ=255)
                                                                         tgAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGa                           >  2:4047029/1‑100 (MQ=255)
                                                                             cagcaAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGcc                       <  2:2170274/100‑1 (MQ=255)
                                                                               gcaAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGa                     >  2:670229/1‑100 (MQ=255)
                                                                               gcaAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGAAGGCGGTATGAGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGa                     >  1:2973971/1‑100 (MQ=255)
                                                                                  aaGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGAttt                  <  2:3482895/100‑1 (MQ=255)
                                                                                     tcatttaacTGCGCACGCAGTTAAATGAACGTATTCTGGTGCTGGCCGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCg               >  1:1760125/7‑100 (MQ=255)
                                                                                     tcatttaacTGCGCACGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCg               >  2:1568770/7‑100 (MQ=255)
                                                                                     tGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCg               <  2:1740350/100‑1 (MQ=255)
                                                                                      ggAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgt              >  1:703727/1‑100 (MQ=255)
                                                                                      catttaacTGCGCACGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgt              >  2:854786/6‑100 (MQ=255)
                                                                                      catttaacTGCGCACGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAACCGATTTTCgt              >  1:1556662/6‑100 (MQ=255)
                                                                                      catttaacTGCGCACGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATAGACTGAACGAAGCCGATTTTCgt              >  1:3630603/6‑100 (MQ=255)
                                                                                       gAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtg             <  2:3792302/100‑1 (MQ=255)
                                                                                       atttaacTGCGCACGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtg             >  2:1625181/5‑100 (MQ=255)
                                                                                       atttaacTGCGCACGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtg             >  1:1220440/5‑100 (MQ=255)
                                                                                       atttaacTGCGCACGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtg             >  1:3468984/5‑100 (MQ=255)
                                                                                       atttaacTGCGCACGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtg             >  1:3893587/5‑100 (MQ=255)
                                                                                       atttaacTGCGCACGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtg             >  2:706284/5‑100 (MQ=255)
                                                                                        aacaacTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtgg            >  1:3672314/1‑100 (MQ=255)
                                                                                        aacaacTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtgg            >  2:1930463/1‑100 (MQ=255)
                                                                                        aacaacTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtgg            >  1:534741/1‑100 (MQ=255)
                                                                                          taacTGCGCACGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtggtg          >  1:1105983/2‑100 (MQ=255)
                                                                                          caacTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtggtg          >  1:321827/1‑100 (MQ=255)
                                                                                            acTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGaa        >  1:3100585/1‑100 (MQ=255)
                                                                                               gcgtgcgCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGc     <  1:2733211/100‑1 (MQ=255)
                                                                                                cgtgcgCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCt    >  1:454701/1‑100 (MQ=255)
                                                                                                 gtgcgCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCtt   <  2:2863490/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
CACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTT  >  NC_000913/4223756‑4223953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: