Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 569,160 G→T 16.9% D87Y (GAC→TAC) ‡ ybcK → DLP12 prophage; putative recombinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913569,1600GT16.9% 489.7 / 79.4 189D87Y (GAC→TAC) ybcKDLP12 prophage; putative recombinase
Reads supporting (aligned to +/- strand):  ref base G (77/79);  new base T (21/11);  total (99/90)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01

Only 100 of 199 total aligned reads displayed.

AAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCT  >  NC_000913/569061‑569259
                                                                                                   |                                                                                                   
aaaGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTgg                                                                                                     <  2:859668/100‑1 (MQ=255)
aaaGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTgg                                                                                                     <  2:332678/100‑1 (MQ=255)
 aaGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGa                                                                                                    >  2:137871/1‑100 (MQ=255)
 aaGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGa                                                                                                    <  2:3572648/100‑1 (MQ=255)
   ggAAAGCATGCACAATCAGGAGCTTTTTTTTTTTTTTTTTATGCTATAGAACATGGTTATATATTGCCAGGTACTACATTGTTAGTTGAAAGTCTGTACa                                                                                                  >  1:1977751/1‑100 (MQ=255)
   ggAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACa                                                                                                  >  2:3402747/1‑100 (MQ=255)
     aaaGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGa                                                                                                <  2:3889655/100‑1 (MQ=255)
       aGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACt                                                                                              <  1:2818976/100‑1 (MQ=255)
          aTGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTc                                                                                           <  1:1290813/100‑1 (MQ=255)
          aTGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTc                                                                                           >  1:1999792/1‑100 (MQ=255)
           tGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCa                                                                                          <  1:3222923/100‑1 (MQ=255)
            gCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaa                                                                                         <  2:3788393/100‑1 (MQ=255)
             cacaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaac                                                                                        <  2:1710342/100‑2 (MQ=255)
             cacaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaac                                                                                        <  1:1421537/100‑2 (MQ=255)
             cacaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaac                                                                                        <  2:1605572/100‑2 (MQ=255)
             cacaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAag                                                                                        <  1:3591178/100‑1 (MQ=255)
             cacaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAag                                                                                        >  2:1401371/1‑100 (MQ=255)
              acaagtaGGAGCTTTTTCGGAATTTTTAAATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAaga                                                                                       <  1:2599009/94‑1 (MQ=255)
              acaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAaga                                                                                       <  1:3056774/100‑1 (MQ=255)
              acaATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAaga                                                                                       >  2:4100481/1‑100 (MQ=255)
               caATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAagag                                                                                      >  2:1483745/1‑100 (MQ=255)
                   cAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATCGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGaaaa                                                                                  <  2:489144/100‑1 (MQ=255)
                     ggAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAg                                                                                >  1:265383/1‑100 (MQ=255)
                     ggAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAg                                                                                >  1:547885/1‑100 (MQ=255)
                       aGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTc                                                                              <  2:3712005/100‑1 (MQ=255)
                        gCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCg                                                                             >  2:40666/1‑100 (MQ=255)
                          tttttCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGt                                                                           <  2:569492/100‑1 (MQ=255)
                          tttttCGGAATTTTTAGATGCTATAGAGCATGATTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGt                                                                           <  1:3237590/100‑1 (MQ=255)
                           ttttCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTg                                                                          <  2:3279220/100‑1 (MQ=255)
                            tttCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGa                                                                         <  2:2005503/100‑1 (MQ=255)
                            tttCGGAATTTTTAGATACTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGATGAAATTCTGAACAGAGTTTCAAGAGAAAAAGTCGGTg                                                                          >  1:1057109/1‑99 (MQ=255)
                               cGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGc                                                                      >  2:1918058/1‑100 (MQ=255)
                               cGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGc                                                                      >  2:2448699/1‑100 (MQ=255)
                                ggAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCg                                                                     <  2:1004789/100‑1 (MQ=255)
                                ggAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCg                                                                     <  2:1400026/100‑1 (MQ=255)
                                 gAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGa                                                                    <  2:1344404/100‑1 (MQ=255)
                                 gAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGa                                                                    >  1:118858/1‑100 (MQ=255)
                                  aaTTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGAt                                                                   >  2:3072936/1‑100 (MQ=255)
                                  aaTGTTTAGATGCTATAGAGTATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGAt                                                                   <  2:3088542/100‑1 (MQ=255)
                                    tttttAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTg                                                                 >  2:623562/1‑100 (MQ=255)
                                    tttttAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTg                                                                 >  2:1168967/1‑100 (MQ=255)
                                        tAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACg                                                             <  1:79655/100‑1 (MQ=255)
                                          gATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACg                                                             <  1:1903176/98‑1 (MQ=255)
                                          gATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGtn                                                           <  2:2119276/100‑2 (MQ=255)
                                          gATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTc                                                           <  2:2745519/100‑1 (MQ=255)
                                           aTGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCt                                                          <  1:713150/100‑1 (MQ=255)
                                           aTGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCt                                                          <  1:1841467/100‑1 (MQ=255)
                                             gCTCTAGAGCATGGTTATATATTGCCAGGAACTCCACTGTTAGTTGAAAGTCTGGACAGACTTTAAAGAGAAAAAGTCGGTGAAGCGATGGAACGtgtg                                                         >  1:2539315/1‑99 (MQ=255)
                                             gCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGa                                                        >  1:924866/1‑100 (MQ=255)
                                                ataGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGaaa                                                      <  2:654907/99‑1 (MQ=255)
                                                     gCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGAtt                                                >  2:4388795/1‑100 (MQ=255)
                                                          gTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGaa                                           >  2:422646/1‑100 (MQ=255)
                                                              tatatTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCAc                                       <  2:898231/100‑1 (MQ=255)
                                                               atatTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACg                                      <  2:1179335/100‑1 (MQ=255)
                                                               atatTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACg                                      <  1:1150695/100‑1 (MQ=255)
                                                                tatTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACgg                                     <  1:137871/100‑1 (MQ=255)
                                                                tatTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACgg                                     >  1:471133/1‑100 (MQ=255)
                                                                    gCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTAtt                                 >  1:2282952/1‑100 (MQ=255)
                                                                      cAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGa                               >  2:61277/1‑100 (MQ=255)
                                                                       aGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGAt                              <  2:2583150/100‑1 (MQ=255)
                                                                       aGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGAt                              >  2:1276803/1‑100 (MQ=255)
                                                                        ggAACTACATTGTTAGTTGAAAGTCTGGCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATg                             >  1:2755951/1‑100 (MQ=255)
                                                                        ggAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATg                             >  2:3930249/1‑100 (MQ=255)
                                                                          aaCTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGtt                           <  2:3497680/100‑1 (MQ=255)
                                                                            cTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTtat                         <  1:4277708/100‑1 (MQ=255)
                                                                             tACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTtata                        <  2:4023649/100‑1 (MQ=255)
                                                                             tACATTGTGAGTGGAAAGTCTGGACAGTCTTTCGAGAGAAAATGTGGGGGAATCGATTGAACGTCTGAAAGTGATGGTGGAGCACGGTATTGATGTtata                        >  1:1466507/1‑100 (MQ=255)
                                                                              aCATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATaa                       >  2:2037788/1‑100 (MQ=255)
                                                                              aCATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATaa                       >  2:630399/1‑100 (MQ=255)
                                                                               cATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAAc                      <  2:4300792/100‑1 (MQ=255)
                                                                               cATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAAc                      <  2:562132/100‑1 (MQ=255)
                                                                                nttGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAAct                     >  1:3840856/2‑100 (MQ=255)
                                                                                 ttttctcttGAAAGTCAGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGAGATTGAACGTTTGAAATTAATTTTGAATCACGGTATGGATGTTAAAAc                      >  1:78512/8‑98 (MQ=25)
                                                                                  tGTTAGTTGAAAGTCTGGATAGACTTTCTAGAGAAAAAGTTGGTGAAGCGATTGAACTTCTGAAATTGATTTTGGATAACGGTATTGATGTTATaa                       >  1:818834/1‑96 (MQ=255)
                                                                                   ttctcttGAAAGTATGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCtt                  >  1:2725803/6‑100 (MQ=38)
                                                                                   gTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCtt                  >  2:2959869/1‑100 (MQ=255)
                                                                                      aGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGc               <  1:3306608/100‑1 (MQ=255)
                                                                                       gTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCg              >  2:2292069/1‑100 (MQ=255)
                                                                                       gTTGAAAGTCTGGAAAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATAGAACGTCTGAAATTAATTTTGAATCAAGGTATTAATGTTATAActatatg                >  1:3803755/1‑93 (MQ=255)
                                                                                       cttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCttt                 >  1:2330116/2‑97 (MQ=255)
                                                                                       cttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGc               >  1:4370283/2‑99 (MQ=255)
                                                                                       cttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCg              >  2:3417271/2‑100 (MQ=255)
                                                                                       cttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCg              >  2:633910/2‑100 (MQ=255)
                                                                                       cttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCg              >  1:3552526/2‑100 (MQ=255)
                                                                                       cttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCg              >  1:662179/2‑100 (MQ=255)
                                                                                       cttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCg              >  2:4122569/2‑100 (MQ=255)
                                                                                       cttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCAAGGTATTGATGTTATAACTCTTTGCg              >  1:1081709/2‑100 (MQ=255)
                                                                                         tGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGAc            <  2:4344735/100‑1 (MQ=255)
                                                                                         tGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGAc            <  1:1805568/100‑1 (MQ=255)
                                                                                         tGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGAc            >  2:3609936/1‑100 (MQ=255)
                                                                                         tGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGAc            >  2:3173642/1‑100 (MQ=255)
                                                                                          gAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACa           <  2:2838822/100‑1 (MQ=255)
                                                                                           aaaGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACaa          >  2:3451762/1‑100 (MQ=255)
                                                                                            aaGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAAt         >  1:4063482/1‑100 (MQ=255)
                                                                                                 tGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGt    >  2:254511/1‑100 (MQ=255)
                                                                                                  ggACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGt    >  1:3628657/1‑99 (MQ=255)
                                                                                                  ggACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTc   >  1:2635732/1‑100 (MQ=255)
                                                                                                  ggACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTc   <  1:1636184/100‑1 (MQ=255)
                                                                                                  ggACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTc   <  1:3914675/100‑1 (MQ=255)
                                                                                                   gACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCt  <  2:4316945/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
AAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCT  >  NC_000913/569061‑569259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: