Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 693,030 C→A 15.3% V117L (GTA→TTA)  ybeZ ← heat shock protein, putative NTPase; PhoH‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913693,0300CA15.3% 408.9 / 51.9 150V117L (GTA→TTA) ybeZheat shock protein, putative NTPase; PhoH‑like protein
Reads supporting (aligned to +/- strand):  ref base C (59/65);  new base A (7/16);  total (69/81)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 166 total aligned reads displayed.

GGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTG  >  NC_000913/692932‑693129
                                                                                                  |                                                                                                   
ggtTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTAcg                                                                                                    >  2:2202556/1‑100 (MQ=255)
ggtTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTAcg                                                                                                    >  1:4411779/1‑100 (MQ=255)
 gtTTTACCCGTACCCGCCGGGTCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCGTAATTAagc                                                                                                   <  1:1941965/100‑1 (MQ=255)
 gtTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGCTGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTAcgc                                                                                                   >  1:3007467/1‑100 (MQ=255)
 gtTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTAcgc                                                                                                   >  1:3559107/1‑100 (MQ=255)
 gtTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTAcgc                                                                                                   >  2:2086552/1‑100 (MQ=255)
 gtTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTAcgc                                                                                                   >  2:1972599/1‑100 (MQ=255)
 gtTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTAcgc                                                                                                   >  2:4380227/1‑100 (MQ=255)
   tttACCCATACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCTATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTAcgccg                                                                                                 >  1:3098521/1‑100 (MQ=255)
     tacccgtacccgCCGGGGCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGCTGTACTGCGTCTGGTTTGGCGTGCGCGGCTTAATTATGCcgcg                                                                                               >  2:2450595/1‑100 (MQ=255)
      acccgtacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGt                                                                                              >  1:1989489/1‑100 (MQ=255)
        ccgtacccgCTGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTTGTTTGGCGTGCGCGGCGTAATTAAGCcgcgcac                                                                                            <  1:2898073/100‑4 (MQ=255)
        ccgtacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCGTAATTAAGCcgcgcac                                                                                            <  1:101548/100‑4 (MQ=255)
        ccgtacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCGTAATTAAGCcgcgcac                                                                                            <  1:3559955/100‑4 (MQ=255)
        ccgtacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCGTAATTAAGCcgcgcac                                                                                            <  1:90872/100‑4 (MQ=255)
         cgtacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTg                                                                                           >  1:3165608/1‑100 (MQ=255)
         cgtacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCGTAATTAAGCcgcgcacg                                                                                           <  2:1260753/100‑5 (MQ=255)
         cgtacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCGTAATTAAGCcgcgcacg                                                                                           <  2:391157/100‑5 (MQ=255)
         cgtacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCGTAATTAAGCcgcgcacg                                                                                           <  2:3621678/100‑5 (MQ=255)
         cgtacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCGTAATTAAGCcgcgcacg                                                                                           <  2:2587983/100‑5 (MQ=255)
         cgtacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTGTGGCGTGCGCGGCTTAATTACGCCGCGTTTg                                                                                           >  2:3706678/1‑100 (MQ=255)
          gtacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTgg                                                                                          <  1:2954317/100‑1 (MQ=255)
          gtacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCGTAATTAAGCcgcgcacgc                                                                                          <  1:3712791/100‑6 (MQ=255)
           tacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCGTAATTAAGCcgcgcacgcc                                                                                         <  1:3116153/100‑7 (MQ=39)
            acccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGtt                                                                                        >  2:3977986/1‑100 (MQ=255)
            acccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGtt                                                                                        >  2:2436277/1‑100 (MQ=255)
             ccggccgGGCCAACGCCTAAGGTAATGTCCTGGTCGCGCATATTCCCGACGTGTTGCGACTGGGCTGGCGTGACCGGCTTACTTCCGTCGCGCTTgcat                                                                                        >  1:3377866/4‑96 (MQ=255)
             cccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGttt                                                                                       <  1:3749217/100‑1 (MQ=255)
              ccgccgGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGtttt                                                                                      >  1:862340/1‑100 (MQ=255)
               cgccgGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTg                                                                                     >  1:4372894/1‑100 (MQ=255)
               cgccgGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTg                                                                                     <  2:2621156/100‑1 (MQ=255)
                gccgGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGa                                                                                    <  1:3327512/100‑1 (MQ=255)
                gccgGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGa                                                                                    <  2:1248479/100‑1 (MQ=255)
                  cgGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGata                                                                                  >  2:1710231/1‑100 (MQ=255)
                  cgGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGata                                                                                  <  2:4285084/100‑1 (MQ=255)
                  cgGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGata                                                                                  <  1:2753822/100‑1 (MQ=255)
                   gggCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGatat                                                                                 >  1:791962/1‑100 (MQ=255)
                     gCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTg                                                                               >  1:981964/1‑100 (MQ=255)
                     gCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTg                                                                               >  1:1256497/1‑100 (MQ=255)
                     gCCAAAGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTg                                                                               <  1:3790145/100‑1 (MQ=255)
                      ccAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGa                                                                              <  1:1156683/100‑1 (MQ=255)
                       cAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGAc                                                                             >  2:2875228/1‑100 (MQ=255)
                          cGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTAGGCCGCGTTTGGTTTTGCTATTGACCGc                                                                          >  1:2030838/1‑100 (MQ=255)
                             cGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCttt                                                                       >  2:3849280/1‑100 (MQ=255)
                             cGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCttt                                                                       >  1:2746222/1‑100 (MQ=255)
                                 ggTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCg                                                                   <  1:567060/100‑1 (MQ=255)
                                 ggTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCg                                                                   >  2:3552544/1‑100 (MQ=255)
                                 ggTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCg                                                                   <  1:3693851/100‑1 (MQ=255)
                                    aaTGTCATGGACGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTAc                                                                <  1:1962298/100‑1 (MQ=255)
                                      tGTCATTGTGGAGAATATTGGCGATGTACTGCGCCTGTTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTc                                                              <  1:976317/100‑1 (MQ=255)
                                          aTGGTCGAGAATATTGGCGACGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGc                                                          >  1:2703168/1‑100 (MQ=255)
                                           tGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCa                                                         <  2:3422181/100‑1 (MQ=255)
                                           tGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCa                                                         >  2:843962/1‑100 (MQ=255)
                                           tGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCa                                                         <  1:1364787/100‑1 (MQ=255)
                                             gTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACg                                                       >  1:3211189/1‑100 (MQ=255)
                                                 agaATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGctct                                                   <  1:1903639/100‑1 (MQ=255)
                                                   aaTATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTcc                                                 <  2:1549843/100‑1 (MQ=255)
                                                    atatTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCcg                                                <  1:799311/100‑1 (MQ=255)
                                                    atatTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCcg                                                <  2:3218101/100‑1 (MQ=255)
                                                      atTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCcgcg                                              <  1:705257/100‑1 (MQ=255)
                                                       ttGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCgcgc                                             >  1:230268/1‑100 (MQ=255)
                                                          gCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCttt                                          >  2:1190121/1‑100 (MQ=255)
                                                          gCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCttt                                          <  1:3197521/100‑1 (MQ=255)
                                                          gCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCtt                                           <  1:2844306/99‑1 (MQ=255)
                                                           cGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTg                                         >  2:4017057/1‑100 (MQ=255)
                                                            gATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGc                                        <  1:2299948/100‑1 (MQ=255)
                                                               gTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTcc                                     <  1:408014/100‑1 (MQ=255)
                                                                 aCTGCGCCTGTTTTGGCTTCCGCAGCTTAATTCCTCCGCGGTCCGGTTTGATATTAACCGCTTTGCCGTACTCCGCCACGCTCTCCGCGCTTTGCCCCAg                                   <  1:3611239/100‑1 (MQ=255)
                                                                 aCTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCGGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAg                                   >  1:2934001/1‑100 (MQ=255)
                                                                 aCTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAg                                   <  2:1343466/100‑1 (MQ=255)
                                                                   tGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTa                                 >  2:4124970/1‑100 (MQ=255)
                                                                    gcgcCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTAc                                >  1:4213093/1‑100 (MQ=255)
                                                                     cgcCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTAcc                               <  2:265649/100‑1 (MQ=255)
                                                                     cgcCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTAcc                               <  2:1544885/100‑1 (MQ=255)
                                                                      gcCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTAccc                              >  1:1591463/1‑100 (MQ=255)
                                                                       ccTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCcg                             <  2:2221078/100‑1 (MQ=255)
                                                                          ggTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCgcgc                          >  2:2242765/1‑100 (MQ=255)
                                                                          ggTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCgcgc                          >  2:42135/1‑100 (MQ=255)
                                                                           gTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGct                         >  1:1870925/1‑100 (MQ=255)
                                                                           gTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGct                         <  2:3165626/100‑1 (MQ=255)
                                                                           caaaaccaaacgcgGCGTAATTAAGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGct                         >  2:928587/11‑100 (MQ=38)
                                                                             ttGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGcttc                       <  2:2808898/100‑1 (MQ=255)
                                                                              tGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTCTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGcttct                      <  2:3545639/100‑1 (MQ=255)
                                                                                gCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCttt                    <  2:54738/100‑1 (MQ=255)
                                                                                 caaacgcgGCGTAATTAAGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTa                   >  1:2067473/5‑100 (MQ=255)
                                                                                  gTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTaa                  >  1:355050/1‑100 (MQ=255)
                                                                                  gTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTaa                  <  1:3989612/100‑1 (MQ=255)
                                                                                  aaacgcgGCGTAATTAAGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTaa                  >  2:4146307/4‑100 (MQ=255)
                                                                                   tGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAAt                 >  2:4406693/1‑100 (MQ=255)
                                                                                    gcgcGGCTTAATTAGGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGGGCTTTGCTCCAGTACCCGCGCTTCTTTAATc                >  1:3672473/1‑100 (MQ=255)
                                                                                    gcgcGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATc                <  2:835194/100‑1 (MQ=255)
                                                                                     cgcgGCTTAATTACGCCGCGGTTGGTTTTGAGATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTGTAATCg               <  1:703125/100‑1 (MQ=255)
                                                                                           ttAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGTTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGt         >  2:1980501/1‑100 (MQ=255)
                                                                                            tAATCACGCCGCTTTTAGATCTGATATTGACCCCTTTGCCTTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTg        <  2:2569883/100‑1 (MQ=255)
                                                                                               ttACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGAt     >  2:2305124/1‑100 (MQ=255)
                                                                                               ttACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGAt     >  1:827021/1‑100 (MQ=255)
                                                                                                tACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATc    >  1:121580/1‑100 (MQ=255)
                                                                                                  cgccgcGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGTCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTg  <  1:4077659/100‑1 (MQ=255)
                                                                                                  cgccgcGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTg  >  1:942636/1‑100 (MQ=255)
                                                                                                  cgccgcGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTg  >  1:1554287/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
GGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTG  >  NC_000913/692932‑693129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: