BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	805,312	G→T	16.7%	E604* (GAG→TAG) ‡	ybhJ →	aconitase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	805,312	0	G	T	16.7%	499.0 / 81.8	190	E604* (GAG→TAG)	ybhJ	aconitase family protein
Reads supporting (aligned to +/- strand): ref base G (78/80); new base T (12/20); total (90/100)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01

Only 100 of 192 total aligned reads displayed.

CTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGA  >  NC_000913/805213‑805408

cTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCTGGGAATGTCAGCg                                                                                                  <  1:2728084/100‑1 (MQ=255)

  ggCGGAGTTTGCCCGGTCGCGCCGCGATCCCGATTATGTTAGCAGAAGTAAAGCGCCTGCTCAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAg                                                                                                <  1:1124385/100‑1 (MQ=255)

  ggCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAg                                                                                                >  1:399704/1‑100 (MQ=255)

  ggCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAg                                                                                                >  1:1705727/1‑100 (MQ=255)

   gCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGc                                                                                               <  1:4263991/100‑1 (MQ=255)

   gCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGc                                                                                               <  1:1305326/100‑1 (MQ=255)

    cGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCt                                                                                              <  1:3667736/100‑1 (MQ=255)

     ggAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTg                                                                                             <  2:119336/100‑1 (MQ=255)

       aGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGAc                                                                                           >  1:3426424/1‑100 (MQ=255)

          ttACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCTCGCTGACatt                                                                                        <  2:2055554/100‑3 (MQ=255)

           tACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCTCGCTGACattc                                                                                       <  2:1294807/100‑4 (MQ=255)

           tACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCTCGCTGACattc                                                                                       <  2:2246593/100‑4 (MQ=255)

           tACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACagag                                                                                       <  2:2888048/100‑1 (MQ=255)

            aCCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGACAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAgg                                                                                      >  1:2017350/1‑100 (MQ=255)

             cccTGTCTCGCCGCGGTCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCTCGCTGACattcc                                                                                      <  1:4305315/99‑5 (MQ=255)

             cccTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCTCGCTGACattccc                                                                                     <  1:4293944/100‑6 (MQ=255)

             cccTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGgt                                                                                     >  1:868639/1‑100 (MQ=255)

             cccTGTCGCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAgg                                                                                      <  1:2911783/99‑1 (MQ=255)

              ccTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCTCGCTGACattcccc                                                                                    <  1:2896353/100‑7 (MQ=255)

              ccTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGgtg                                                                                    <  1:1546257/100‑1 (MQ=255)

                tGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGtt                                                                                  <  2:1357921/100‑1 (MQ=255)

                  tctctgcgcgACCACGGTTATGTTAGCAGAAGTAAAGTGACTGCTGAGCTGGAAAATGAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTg                                                                                <  1:3652850/94‑1 (MQ=255)

                  tctcGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTg                                                                                <  1:2469364/100‑1 (MQ=255)

                   ctcGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGCATGTCAGCGAGCTGACAGAGGTGTTTgc                                                                               >  2:1064217/1‑100 (MQ=255)

                   ctcGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTgc                                                                               >  2:3025493/1‑100 (MQ=255)

                   ctcGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTgc                                                                               >  1:4200443/1‑100 (MQ=255)

                    tcGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTgcg                                                                              >  2:111617/1‑100 (MQ=255)

                      gccgcGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTgcgcg                                                                            <  2:4333918/100‑1 (MQ=255)

                      gacgcgATCCCGGCTATGTTAGCTGAAGTAAAGCGCCTGCTGAGCTGGACAAACAGCCTCTGGCGGGGAATGTCAGGGAGCTGACAGAGGCGTTTgcgc                                                                             >  1:3498868/3‑99 (MQ=255)

                       ccgcGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTgcgcgc                                                                           >  1:3537639/1‑100 (MQ=255)

                       ccgcGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTgcgcgc                                                                           <  2:4191249/100‑1 (MQ=255)

                        cgcgATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCa                                                                          >  1:3715525/1‑100 (MQ=255)

                          cgATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCAtt                                                                        >  2:1835759/1‑100 (MQ=255)

                           gATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTa                                                                       >  2:2572230/1‑100 (MQ=255)

                            aTCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTaa                                                                      >  1:1315216/1‑100 (MQ=255)

                             tCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAg                                                                     <  2:1833454/100‑1 (MQ=255)

                              cccGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTaa                                                                      <  2:3088780/98‑1 (MQ=255)

                              cccGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAg                                                                     <  1:1269803/99‑1 (MQ=255)

                               ccGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCa                                                                   <  2:1331927/100‑1 (MQ=255)

                               ccGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCa                                                                   >  2:234596/1‑100 (MQ=255)

                                ccgTTATGTTAGCGGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAg                                                                  >  2:2971114/3‑100 (MQ=255)

                                 ggTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGa                                                                 >  1:1147397/1‑100 (MQ=255)

                                 ggTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGa                                                                 >  2:1132045/1‑100 (MQ=255)

                                      tGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCt                                                            >  2:3725176/1‑100 (MQ=255)

                                       gTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTg                                                           <  2:3629194/100‑1 (MQ=255)

                                       gTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTg                                                           <  2:743905/100‑1 (MQ=255)

                                       gTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTg                                                           >  1:2684795/1‑100 (MQ=255)

                                        ttAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTgg                                                          >  1:4115841/1‑100 (MQ=255)

                                          ttccgatctAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCACATTAAGCAGATTGCTGGTc                                                        <  1:3102060/92‑1 (MQ=255)

                                            cAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGAGAGAGGTGTTTGCGCGCATTAAGCAGATTTCTGGTCa                                                       >  1:2051524/1‑99 (MQ=255)

                                            cAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAg                                                      >  2:2087871/1‑100 (MQ=255)

                                             aGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAgg                                                     <  1:675816/100‑1 (MQ=255)

                                              gAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGa                                                    >  1:391381/1‑100 (MQ=255)

                                               aaGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAg                                                   <  2:3713561/100‑1 (MQ=255)

                                                 gTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCa                                                 <  1:2782787/100‑1 (MQ=255)

                                                   aaaGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCata                                               <  2:3548998/100‑1 (MQ=255)

                                                   aaaGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGAGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCACATTAAGCAGATTGCTGGTCAgatcggaa                                               >  2:3102078/1‑93 (MQ=255)

                                                     aGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATAtt                                             >  1:2605242/1‑100 (MQ=255)

                                                      gCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTg                                            >  1:434990/1‑100 (MQ=255)

                                                       cGACTGCTGAGCTGGTAAATCAGCGTCTGGCGGCGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGCAAGGGGCat                                                <  1:2066844/95‑1 (MQ=255)

                                                        gACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGa                                           >  1:3811523/1‑99 (MQ=255)

                                                        gACTGCTGAGCAGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGAt                                          >  1:2306280/1‑100 (MQ=255)

                                                         aCTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATc                                         <  1:2553988/100‑1 (MQ=255)

                                                          ctgctgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATcc                                        <  1:3244876/100‑1 (MQ=255)

                                                          ctgctgAGCTGGAAAAGTAGCATCTGGAGCGGAATGTCAGCGGGCTGACAGAGATGTTTGCGTCCACTAAGGAGATTGCTTGTAAGGATTTTATTGATcc                                        <  1:2647851/100‑1 (MQ=255)

                                                            gctgAGCTGGACCATAAGCGTCTGGCGGGGAATGTCAGCGAGTTGACCGACGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGc                                      <  2:2404135/100‑1 (MQ=255)

                                                            gctgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGc                                      <  1:3277247/100‑1 (MQ=255)

                                                             ctgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCt                                     >  2:1554704/1‑100 (MQ=255)

                                                             ctgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCt                                     <  2:2130849/100‑1 (MQ=255)

                                                             ctgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCt                                     >  2:2225394/1‑100 (MQ=255)

                                                              tgggCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCt                                     <  1:19192/96‑1 (MQ=255)

                                                              tgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTg                                    <  1:798160/100‑1 (MQ=255)

                                                              tgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTg                                    <  2:3820809/100‑1 (MQ=255)

                                                              tgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTg                                    <  2:2603815/100‑1 (MQ=255)

                                                                 gCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCACTGCaa                                 <  1:383781/100‑1 (MQ=255)

                                                                  cTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCaaa                                >  2:3645619/1‑100 (MQ=255)

                                                                    ggAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACt                              >  1:766299/1‑100 (MQ=255)

                                                                      aaaaTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGTGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGa                            >  1:574666/1‑100 (MQ=255)

                                                                      aaaaTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGa                            <  2:2012100/100‑1 (MQ=255)

                                                                      aaaaTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATCGATCCGCTGCAAACTGa                            <  2:1705734/100‑1 (MQ=255)

                                                                      aaaaTCAGCGCCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGTGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGa                            >  1:2388883/1‑100 (MQ=255)

                                                                        aaTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGaaa                          <  2:4380277/100‑1 (MQ=255)

                                                                         aTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAAt                         <  2:3205990/100‑1 (MQ=255)

                                                                           cAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTg                       <  2:2684810/100‑1 (MQ=255)

                                                                           cAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTg                       <  2:4010660/100‑1 (MQ=255)

                                                                                 cTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgcagca                 >  2:940905/1‑100 (MQ=255)

                                                                                 cTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgcagca                 >  2:2486146/1‑100 (MQ=255)

                                                                                   ggCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCTCATTAAGCAGATTGCTGGTTAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATg               <  1:2403223/100‑1 (MQ=255)

                                                                                   ggCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATg               >  1:959384/1‑100 (MQ=255)

                                                                                    gCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATgg              <  1:946031/100‑1 (MQ=255)

                                                                                      ggggAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTc            >  2:600189/1‑100 (MQ=255)

                                                                                        cctctGTCAGCTCGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTa          >  2:295156/5‑100 (MQ=255)

                                                                                        cctctGTCAGCTCGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTa          >  2:2758208/5‑100 (MQ=255)

                                                                                        cctctGTCAGCTCGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTa          >  2:2426717/5‑100 (MQ=255)

                                                                                          aaTGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTAt         <  1:3305470/99‑1 (MQ=255)

                                                                                          aaTGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATg        <  2:1511912/100‑1 (MQ=255)

                                                                                             gTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCgg     <  2:4078/100‑1 (MQ=255)

                                                                                               cAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTg   <  2:3314442/100‑1 (MQ=255)

                                                                                               cAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTg   <  1:4061814/100‑1 (MQ=255)

                                                                                                aGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGa  <  1:3378765/100‑1 (MQ=255)

CTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGA  >  NC_000913/805213‑805408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`