Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,211,614 C→T 16.6% intergenic (+37/+66) icdC → / ← iraM pseudogene, isocitrate dehydrogenase C‑terminal gene fragment; idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion/RpoS stabilzer during Mg starvation, anti‑RssB factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,211,6140CT16.6% 399.9 / 58.4 163intergenic (+37/+66)icdC/iraMpseudogene, isocitrate dehydrogenase C‑terminal gene fragment; idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion/RpoS stabilzer during Mg starvation, anti‑RssB factor
Reads supporting (aligned to +/- strand):  ref base C (77/58);  new base T (15/12);  total (93/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 171 total aligned reads displayed.

ATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATG  >  NC_000913/1211515‑1211713
                                                                                                   |                                                                                                   
aTGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAac                                                                                                     >  1:4042372/1‑100 (MQ=255)
    aaGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGc                                                                                                 >  2:3798409/1‑100 (MQ=255)
     aGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGcc                                                                                                <  2:3713458/100‑1 (MQ=255)
      ggCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGccc                                                                                               <  1:302620/100‑1 (MQ=255)
        gcgcaaGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTGTTATACGCCCgt                                                                                             <  1:1218386/96‑1 (MQ=25)
         gcTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTGTTATACGCCCgtt                                                                                            <  2:7824/100‑1 (MQ=25)
         gcTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCgtt                                                                                            >  2:4029452/1‑100 (MQ=255)
          cTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCgttg                                                                                           <  2:3384702/100‑1 (MQ=255)
          cTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCgttg                                                                                           >  1:3566720/1‑100 (MQ=255)
           tAAGCTGCTGAAATGTTAAGAGTTTGGTGAACCGATCATCGAAAACATGTAATCCCTCCATGTGTTAAATATTGAAACGGGCGTGTTATACGCCCgtttc                                                                                          <  1:709574/100‑3 (MQ=25)
             aGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCgttgttn                                                                                        <  2:283191/100‑2 (MQ=255)
              gctgctGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGtttt                                                                                       <  2:278566/100‑1 (MQ=255)
                tgctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTGTTATACGCCCgtttcaatat                                                                                     <  2:1672389/100‑8 (MQ=25)
                tgctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTtttat                                                                                     <  2:2476137/100‑1 (MQ=255)
                 gctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTtttatt                                                                                    <  1:4148807/100‑1 (MQ=255)
                 gctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTtttatt                                                                                    >  1:167523/1‑100 (MQ=255)
                 gctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTtttatt                                                                                    <  1:3437688/100‑1 (MQ=255)
                 gctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCCCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTtttatt                                                                                    <  2:318076/100‑1 (MQ=255)
                 gctgAAATGTTCAGACTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTtttatt                                                                                    <  2:4343764/100‑1 (MQ=255)
                  ctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTtttattt                                                                                   >  2:1550891/1‑100 (MQ=255)
                   tgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTtttattta                                                                                  >  1:3142043/1‑100 (MQ=255)
                    gAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCATATAACACGCCCGTTGTTttatttat                                                                                 >  1:4371888/1‑100 (MQ=255)
                         gTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGa                                                                            >  2:4016394/1‑100 (MQ=255)
                          ttCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGat                                                                           >  2:2387556/1‑100 (MQ=255)
                          ttCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGat                                                                           >  1:2423224/1‑100 (MQ=255)
                           tCAGAGTTTGGTGAAGCGATGATCGAAAACATGTACTCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGata                                                                          <  2:1252734/100‑1 (MQ=255)
                           tCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGata                                                                          >  2:4016225/1‑100 (MQ=255)
                            nagagTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGatat                                                                         >  1:3230617/2‑100 (MQ=255)
                                tcttGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATattatt                                                                     <  2:4156697/98‑1 (MQ=255)
                                gTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATattatt                                                                     >  2:3197792/1‑100 (MQ=255)
                                gTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATattatt                                                                     >  1:3785215/1‑100 (MQ=255)
                                gTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATattatt                                                                     >  1:1264421/1‑100 (MQ=255)
                                 tttGGTGAAGCGATCATCGAAAACATTTAATCGCTCCATGTGTTAAATATGGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATAttatta                                                                    <  2:367400/100‑1 (MQ=255)
                                 tttGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATAttatta                                                                    <  2:106786/100‑1 (MQ=255)
                                   tGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATAttattaga                                                                  >  1:4156666/1‑98 (MQ=255)
                                       gAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCa                                                              >  1:2367032/1‑100 (MQ=255)
                                            gATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCg                                                         >  2:3375726/1‑100 (MQ=255)
                                              tcatcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAg                                                       <  1:1756743/100‑1 (MQ=255)
                                              tcatcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAg                                                       <  2:6611/100‑1 (MQ=255)
                                               catcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGc                                                      <  1:4293357/100‑1 (MQ=255)
                                               catcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGc                                                      <  2:2649190/100‑1 (MQ=255)
                                                atcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCa                                                     <  2:2954309/100‑1 (MQ=255)
                                                atcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATAGCGAGCa                                                     >  1:3251096/1‑100 (MQ=255)
                                                 tcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCat                                                    >  2:2858581/1‑100 (MQ=255)
                                                 tcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCat                                                    >  2:3709126/1‑100 (MQ=255)
                                                 tcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCat                                                    >  2:386589/1‑100 (MQ=255)
                                                    aaaaCATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATAtt                                                 <  2:1010853/100‑1 (MQ=255)
                                                    aaaaCATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATAtt                                                 >  1:216300/1‑100 (MQ=255)
                                                    aaaaCATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGGTGTTGTATTTATGTGGATATTATTAATAGCATATCGAGCatat                                                  >  2:1101173/1‑99 (MQ=255)
                                                     aaaCATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATAttt                                                <  2:410284/100‑1 (MQ=255)
                                                      aaCATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATGTATGTGGATATTATTAATAGCATATCGAGCATATTta                                               <  1:2355198/100‑1 (MQ=255)
                                                       aCATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTta                                               <  2:1417883/99‑1 (MQ=255)
                                                             aaTCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAg                                        >  2:2082964/1‑100 (MQ=255)
                                                                   tcCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCAtt                                  <  2:3824453/100‑1 (MQ=255)
                                                                        gtgtTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTg                             <  2:4230619/100‑1 (MQ=255)
                                                                        gtgtTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTg                             <  2:119543/100‑1 (MQ=255)
                                                                          gtTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAg                           <  2:2049474/100‑1 (MQ=255)
                                                                          gtTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAg                           >  1:1502061/1‑100 (MQ=255)
                                                                          gtTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAg                           >  2:134028/1‑100 (MQ=255)
                                                                           ttAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGc                          <  2:1458003/100‑1 (MQ=255)
                                                                              aataaaacaaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATTGAGCATATTTATATGAAGCCCATTAATTGAGccc                        >  1:2106834/9‑99 (MQ=25)
                                                                              aaTATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCa                       >  2:1218709/1‑100 (MQ=255)
                                                                                taaaacaaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCata                     >  2:1459996/7‑100 (MQ=38)
                                                                                  ttGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATg                   <  1:346822/100‑1 (MQ=255)
                                                                                  ttGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATg                   >  2:3554333/1‑100 (MQ=255)
                                                                                  ttGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATg                   >  1:3723303/1‑100 (MQ=255)
                                                                                   tGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATgg                  >  2:78503/1‑100 (MQ=255)
                                                                                   tGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATgg                  >  2:924351/1‑100 (MQ=255)
                                                                                   tGAAACGGGCGTATAACACGCCCGTTGTTTTATTCATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAACACATTACTTGAGCCAATATg                   >  1:1008021/1‑99 (MQ=255)
                                                                                   tGAAACGGGCGTATAACACGACCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCAAGCATATTCATCTGAAGACCATTACTTGAGCCCatat                    >  1:1007882/1‑98 (MQ=255)
                                                                                    gAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGTATATTATTATTAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAACTCATATg                   >  1:567823/1‑98 (MQ=255)
                                                                                    acaaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATggg                 <  1:2490680/98‑1 (MQ=255)
                                                                                     caaCGGGCGTGTTATAGGCTAGTTGTTTTATTTATGTGGATATTATTAATAGCATATAGAGCATATTTCTATGAAGTCAATTACTTGAGCCCatattg                  >  1:2612481/2‑96 (MQ=255)
                                                                                     aaaCGGGCGTATAACACGCCCGTTGTTTTATTTATGCGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGc                >  2:3117624/1‑100 (MQ=255)
                                                                                      aaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCa               >  2:463024/1‑100 (MQ=255)
                                                                                      aaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCa               >  2:1585818/1‑100 (MQ=255)
                                                                                      aaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCa               >  2:3028080/1‑100 (MQ=255)
                                                                                      aaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTAATAATAGCATATCGAGCATATTTATATGAAGAGAATTAATTGAGCCCATATGGGCa               >  1:819811/1‑100 (MQ=255)
                                                                                      aaCGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCa               <  2:299847/100‑1 (MQ=255)
                                                                                       aCGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATGTTTATATGAAGCCCATTACTTGAGCCCATATGGGCat              >  2:1980572/1‑100 (MQ=255)
                                                                                        cGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCata             >  2:2917177/1‑100 (MQ=255)
                                                                                        cGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCat              >  1:264114/1‑99 (MQ=255)
                                                                                         gggCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCatat            >  2:3286807/1‑100 (MQ=255)
                                                                                          ggCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATAtt           >  2:3830502/1‑100 (MQ=255)
                                                                                          ggCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATAtt           >  2:1596751/1‑100 (MQ=255)
                                                                                          ggCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATAtt           >  2:2219391/1‑100 (MQ=255)
                                                                                            cGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATAtttt         <  2:1264425/100‑1 (MQ=255)
                                                                                            cGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATAtttt         >  2:833317/1‑100 (MQ=255)
                                                                                             gTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGctcaagtaa        <  2:2797267/100‑9 (MQ=255)
                                                                                             gTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATAttttt        <  1:388094/100‑1 (MQ=255)
                                                                                             gTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATAttttt        <  1:2228226/100‑1 (MQ=255)
                                                                                             gTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATAtttt         <  2:56073/99‑1 (MQ=255)
                                                                                              tataACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTta       >  2:3945458/1‑100 (MQ=255)
                                                                                              tataACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTta       >  2:2189000/1‑100 (MQ=255)
                                                                                               ataACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTtat      >  1:1193009/1‑100 (MQ=255)
                                                                                               ataACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTta       >  1:90417/1‑99 (MQ=255)
                                                                                                taACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTtata     <  1:2952257/100‑1 (MQ=255)
                                                                                                 aaCACGCCCGTTGTTTTATTTATGTAGATATTATTATTAGCATATCGACCATATTTATATGAAGTCAATTACTTATGCCCATATGGGCATATTTATATaa    <  1:1647228/100‑1 (MQ=255)
                                                                                                  acacGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAAt   <  2:2272086/100‑1 (MQ=255)
                                                                                                  acacGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTAATTGACCCAATATGGGCATATTTTTATAAt   >  1:1876552/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
ATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTATAATG  >  NC_000913/1211515‑1211713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑