Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,232,481 C→T 16.3% intergenic (+27/+19) umuC → / ← dsbB translesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,232,4810CT16.3% 409.6 / 64.4 172intergenic (+27/+19)umuC/dsbBtranslesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I
Reads supporting (aligned to +/- strand):  ref base C (78/66);  new base T (11/17);  total (89/83)
Fisher's exact test for biased strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.59e-02

Only 100 of 174 total aligned reads displayed.

TGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCG  >  NC_000913/1232383‑1232578
                                                                                                  |                                                                                                 
  gCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCa                                                                                                >  1:2007934/1‑100 (MQ=255)
   cAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAg                                                                                               <  2:2136413/100‑1 (MQ=255)
   cAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAg                                                                                               <  1:815720/100‑1 (MQ=255)
    aGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAgg                                                                                              >  1:97338/1‑100 (MQ=255)
     gATTAAGCGAGCCATGCTTTCCCCACGTTATACAACGCGAAGTTCTGATTTACTGGGGGTCAAATAAATATATCGGCAGGAAAAAAGCGCTCCCGCAGGa                                                                                             <  1:2867829/100‑1 (MQ=255)
     gATGAAGCGAGCCATGCTTTCACCACGTTATAGAACGCGAGGTTCTGATTTACTGAGGGTCAAATAAATATATCGGCAGGGAAAAAGCGCTCCCGCAGGa                                                                                             >  2:884518/1‑100 (MQ=255)
     gATGAAGCGAGCCATGCTCTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAACAAAGCGCTCCCGCacaa                                                                                             >  2:153339/1‑97 (MQ=255)
        gAAGCGAGCCATGCTTTTACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAgcg                                                                                          <  2:4018612/100‑1 (MQ=255)
        gAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAgcg                                                                                          <  2:763496/100‑1 (MQ=255)
          aGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgct                                                                                        <  2:1523347/100‑2 (MQ=255)
          aGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGcc                                                                                        <  1:1753585/100‑1 (MQ=255)
          aGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGcc                                                                                        <  2:3374315/100‑1 (MQ=255)
           gCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCg                                                                                       <  1:3146254/100‑1 (MQ=255)
            cGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttt                                                                                      <  1:2180938/100‑4 (MQ=255)
             gggacATGCTTTCACCACGTTATACAACGCAAAGTTCTGATTTACTGAGGGTCAAATAAATAAAAAAAAAAAAAAAAAGCGCTCCTGCGGGAgcgctt                                                                                       <  1:1142794/94‑3 (MQ=255)
             gAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgctttt                                                                                     <  2:1510313/100‑5 (MQ=255)
             gAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgctttt                                                                                     <  2:1674819/100‑5 (MQ=255)
             gAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgctttt                                                                                     <  1:3879430/100‑5 (MQ=255)
             gAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgctttt                                                                                     <  1:2798535/100‑5 (MQ=255)
              aGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAAt                                                                                    <  1:33399/100‑1 (MQ=255)
               gCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATg                                                                                   <  1:1615021/100‑1 (MQ=255)
                  aTGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGAt                                                                                <  1:3522368/100‑1 (MQ=255)
                   tGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGAtt                                                                               <  2:1863997/100‑1 (MQ=255)
                    gCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTa                                                                              >  2:4225511/1‑100 (MQ=255)
                     cTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATAAAAAAAAAAAAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAg                                                                             <  1:4262631/100‑1 (MQ=255)
                      tttCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGc                                                                            >  2:3320197/1‑100 (MQ=255)
                       ttCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCg                                                                           <  2:4275792/100‑1 (MQ=255)
                        tCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGa                                                                          >  1:1632447/1‑100 (MQ=255)
                         caccacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGAc                                                                         <  1:593467/100‑1 (MQ=255)
                          accatGTTATACAAAGCCAAGATCTGATTTACTGAGTGTCAAATAAATAAAAAAAAAAAAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGc                                                                            <  1:2082184/96‑1 (MQ=255)
                          accacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGAcc                                                                        <  2:1011956/100‑1 (MQ=255)
                          accacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGAcc                                                                        <  1:427076/100‑1 (MQ=255)
                          accacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGAcc                                                                        <  2:3973966/100‑1 (MQ=255)
                           ccacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCg                                                                       >  2:990602/1‑100 (MQ=255)
                            cacGTTATACTACACGAAGTTCTAGTTTTCTAAGTGTCAAATAAATATAGCAGCAGGAAAAAAGAGCTCCCTCTGGTGCGTGTAATGGATTAGCGACCg                                                                       <  1:3919725/99‑1 (MQ=255)
                              cGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAAc                                                                    >  2:234316/1‑100 (MQ=255)
                               gTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGAcc                                                                        >  1:2948814/1‑95 (MQ=255)
                               gTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAAc                                                                    <  1:614469/99‑1 (MQ=255)
                               gTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAAGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACa                                                                   <  1:289992/100‑1 (MQ=255)
                                ttATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAg                                                                  >  2:2816494/1‑100 (MQ=255)
                                ttATACAACGCGAAGTTCTGATCTACTGAGGTTCAAATAAATAAAGAAGAAAAAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAg                                                                  <  1:2969101/100‑1 (MQ=255)
                                  ataCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGa                                                                 >  1:3482839/1‑99 (MQ=255)
                                  ataCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGAt                                                                <  1:4307439/100‑1 (MQ=255)
                                   taCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGGGCGCCGAATGGATTAGCGACCGAACAGATc                                                               >  2:954972/1‑100 (MQ=255)
                                   taCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATc                                                               >  1:1482067/1‑100 (MQ=255)
                                    aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCa                                                              >  2:3148197/1‑100 (MQ=255)
                                     cAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCAc                                                             >  2:1079148/1‑100 (MQ=255)
                                      aaCGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACg                                                            >  2:1783877/1‑100 (MQ=255)
                                       aCGCGCAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGt                                                           >  2:3244918/1‑100 (MQ=255)
                                         gcgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGACCAGAGCACGttt                                                         >  1:3289094/1‑100 (MQ=255)
                                            aaGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGtttttt                                                      <  2:2044321/100‑1 (MQ=255)
                                             aGTTCTGATTTACTGGGGGTCAAATAAATAAAAAAAAAAAAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTc                                                     <  1:133877/100‑1 (MQ=255)
                                             aGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTc                                                     >  2:699605/1‑100 (MQ=255)
                                              gTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCg                                                    >  1:329687/1‑100 (MQ=255)
                                               ttCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGc                                                   <  1:2849571/100‑1 (MQ=255)
                                                tCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCt                                                  <  2:1556539/100‑1 (MQ=255)
                                                 cTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCtt                                                 >  1:690042/1‑100 (MQ=255)
                                                 cTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCttt                                                >  1:3538096/1‑100 (MQ=255)
                                                  tgatgcacggAGGGTCAAATAAAGATAGCGGCTGGAAAAAAATGCTCGCGCAGAACCGCCGAATGGAGTAACAACCGAACAGATCACGCTTTCTGGCttt                                                >  1:2325015/10‑100 (MQ=255)
                                                   gATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTa                                               >  1:1661036/1‑100 (MQ=255)
                                                   gATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTa                                               >  1:1205907/1‑100 (MQ=255)
                                                       tACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACg                                           >  1:1935506/1‑100 (MQ=255)
                                                           gAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCTCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTg                                       >  1:2383695/1‑100 (MQ=255)
                                                           gAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTg                                       >  2:2419306/1‑100 (MQ=255)
                                                           gAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTg                                       <  1:3898484/100‑1 (MQ=255)
                                                            aGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTgg                                      >  2:2633453/1‑100 (MQ=255)
                                                             gggTCAAATAAATATAGCGGCAGGAAAAACGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTggg                                     <  1:3714967/100‑1 (MQ=255)
                                                             gggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTggg                                     >  2:3041373/1‑100 (MQ=255)
                                                             gggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTggg                                     >  1:89904/1‑100 (MQ=255)
                                                             gggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTggg                                     >  2:1283866/1‑100 (MQ=255)
                                                              ggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAAcagatcgga                                    >  1:2144624/1‑92 (MQ=255)
                                                                tCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGaaa                                  <  1:2653441/100‑1 (MQ=255)
                                                                  aaataaatATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATc                                >  2:3889093/1‑100 (MQ=255)
                                                                   aataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGTTTTAAACGGCTGGGAAATca                               >  1:663653/1‑100 (MQ=255)
                                                                     taaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATcacc                             <  2:2868192/100‑1 (MQ=255)
                                                                      aaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATcacca                            <  1:1679524/100‑1 (MQ=255)
                                                                       aataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATcaccac                           >  2:1360541/1‑100 (MQ=255)
                                                                        atatAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGTTTTAAACGGCTGGGaactaac                              >  1:2275508/1‑91 (MQ=255)
                                                                        atatAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCaccacc                          >  2:2859608/1‑100 (MQ=255)
                                                                        atatAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCaccacc                          <  2:3769542/100‑1 (MQ=255)
                                                                         tataGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCAccacca                         >  2:324391/1‑100 (MQ=255)
                                                                              cGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACt                    <  2:270510/100‑1 (MQ=255)
                                                                               ggCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTg                   >  2:3579990/1‑100 (MQ=255)
                                                                                 cAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCg                 >  1:3579343/1‑100 (MQ=255)
                                                                                   ggAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGAc               >  1:977404/1‑100 (MQ=255)
                                                                                   ggAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGAc               <  2:690014/100‑1 (MQ=255)
                                                                                    gAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACa              >  1:1121787/1‑100 (MQ=255)
                                                                                      attcggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAAt            >  2:892787/6‑100 (MQ=255)
                                                                                      attcggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAAt            >  2:2163684/6‑100 (MQ=255)
                                                                                        aaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCTCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCa          >  1:3958508/1‑100 (MQ=255)
                                                                                         cggcgcTCCTGCGGGAGCGCCGAATGGATTAGTGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAg         >  2:161051/3‑100 (MQ=255)
                                                                                         cggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAg         >  2:557105/3‑100 (MQ=255)
                                                                                         cggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAAAGGCTGGGAAATCACCAACAGCACTGCGACAAt            >  1:3243699/3‑97 (MQ=255)
                                                                                          ggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAgg        >  2:2561632/2‑100 (MQ=255)
                                                                                          ggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAga        >  2:1619843/2‑99 (MQ=255)
                                                                                          aGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAgg        >  1:1783217/1‑100 (MQ=255)
                                                                                            cgcTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTa      >  2:1891141/1‑100 (MQ=255)
                                                                                              cTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTCTAAACGGCTGGGAAATCCCCACCAGCCCTGCGACCACCAGGTa      >  1:1876184/1‑98 (MQ=255)
                                                                                               tCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGc   >  1:1064306/1‑100 (MQ=255)
                                                                                                cccGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCg  >  2:3635745/1‑100 (MQ=255)
                                                                                                  |                                                                                                 
TGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCG  >  NC_000913/1232383‑1232578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: