BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,755,705	(A)_5→4	coding (8/1413 nt)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,755,701	0	A	.	98.4%	573.6 / 1.6	125	coding (4/1413 nt)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (60/63); total (61/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 139 total aligned reads displayed.

CACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCT  >  NC_000913/1755604‑1755795

 accaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaa                                                                                             <  1:3045232/100‑1 (MQ=255)

 accaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaa                                                                                             >  1:202913/1‑100 (MQ=255)

  ccaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaag                                                                                            >  1:1262718/1‑99 (MQ=255)

  ccaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaag                                                                                            <  1:14508/100‑2 (MQ=255)

   caccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaaga                                                                                           <  2:535326/100‑3 (MQ=255)

   caccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaaga                                                                                           >  1:2821413/1‑98 (MQ=255)

    accacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaagac                                                                                          >  2:426351/1‑97 (MQ=255)

     ccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaagacc                                                                                         <  2:76340/100‑5 (MQ=255)

     ccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaagacc                                                                                         >  2:3238893/1‑96 (MQ=255)

     ccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaaga                                                                                           <  2:1677916/98‑3 (MQ=255)

        cTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCaa                                                                                      >  2:712930/1‑99 (MQ=255)

          ttCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAAt                                                                                   >  1:888666/1‑100 (MQ=255)

              tAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTATAGACTAAGACTGTCATG‑AAAAGACCAAAATTGtt                                                                               >  2:2887150/1‑100 (MQ=255)

              tAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGtt                                                                               >  1:2210161/1‑100 (MQ=255)

              tAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGtt                                                                               >  1:2187993/1‑100 (MQ=255)

              gctcttccgATCCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGGCTAAGACTGTCATG‑AAAAGACCAAAATTGtt                                                                               <  2:871637/92‑1 (MQ=255)

               aaTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGttt                                                                              >  1:318803/1‑100 (MQ=255)

               aaTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGttt                                                                              <  2:1501957/100‑1 (MQ=255)

                aTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTg                                                                             <  1:2591553/100‑1 (MQ=255)

                 tACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGc                                                                            >  1:1271025/1‑100 (MQ=255)

                 tACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGc                                                                            <  1:1818150/100‑1 (MQ=255)

                 tACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGc                                                                            >  2:2268789/1‑100 (MQ=255)

                  aCCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCa                                                                           <  1:426352/100‑1 (MQ=255)

                  aCCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCa                                                                           >  2:651221/1‑100 (MQ=255)

                   ccGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCAc                                                                          >  1:2898999/1‑100 (MQ=255)

                    cGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCAcc                                                                         <  2:875381/100‑1 (MQ=255)

                        ttCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCg                                                                     >  1:621193/1‑100 (MQ=255)

                        ttCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCg                                                                     <  1:3192018/100‑1 (MQ=255)

                          cGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCAGCATCGGa                                                                   >  2:332110/1‑100 (MQ=255)

                           gCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGAc                                                                  <  2:1706264/100‑1 (MQ=255)

                           gCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGAc                                                                  <  2:312231/100‑1 (MQ=255)

                            cTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGAcc                                                                 >  1:734071/1‑100 (MQ=255)

                            cTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGAcc                                                                 >  2:518215/1‑100 (MQ=255)

                            cTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGAcc                                                                 <  1:588077/100‑1 (MQ=255)

                            cTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGAcc                                                                 <  1:2268795/100‑1 (MQ=255)

                             tttCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCg                                                                >  1:3179594/1‑100 (MQ=255)

                             tttCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCg                                                                >  1:1592296/1‑100 (MQ=255)

                               tCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGaa                                                              >  2:250235/1‑100 (MQ=255)

                                ccGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGaaa                                                             >  1:3258698/1‑100 (MQ=255)

                                  gacAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCAGCATCGGACCGAAAAc                                                           <  1:332111/98‑1 (MQ=255)

                                  gacAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAAc                                                           <  1:3134161/98‑1 (MQ=255)

                                    cAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCg                                                         <  2:2025486/100‑1 (MQ=255)

                                      gTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGaa                                                       >  1:2182432/1‑100 (MQ=255)

                                       tGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGaa                                                       <  1:410618/100‑1 (MQ=255)

                                       tGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAAt                                                      <  1:380971/100‑1 (MQ=255)

                                         cgcCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCt                                                    >  2:2341301/1‑100 (MQ=255)

                                          gcCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTg                                                   >  2:3400561/1‑100 (MQ=255)

                                           cccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGa                                                  <  2:104621/100‑1 (MQ=255)

                                              aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAaga                                               >  2:2270751/1‑100 (MQ=255)

                                               gAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAagag                                              >  2:2773738/1‑100 (MQ=255)

                                                 aaGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGAt                                            <  2:2256468/100‑1 (MQ=255)

                                                 aaGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGAt                                            <  1:2777368/100‑1 (MQ=255)

                                                 aaGCAAGTGTCGCCCATCCGTGGCAACGTAAAGACTAGTACGGTTATG‑AAATGTCCAAAATTGTTTGCACCATCGTACCGAAAAGCGAATCTGAAGAGAt                                            >  2:1132209/1‑100 (MQ=255)

                                                  aGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATg                                           >  1:833739/1‑100 (MQ=255)

                                                    cAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGtt                                         <  1:1021540/100‑1 (MQ=255)

                                                     aaGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTa                                        <  1:3400574/100‑1 (MQ=255)

                                                     aaGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTa                                        <  1:2436144/100‑1 (MQ=255)

                                                       gTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGc                                      <  2:1262715/100‑1 (MQ=255)

                                                        tttCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCt                                     <  2:1204334/100‑1 (MQ=255)

                                                             cccATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAAt                                >  1:957721/1‑100 (MQ=255)

                                                             cccATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAAt                                >  2:1882525/1‑100 (MQ=255)

                                                               cATCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGc                              <  1:2220623/100‑1 (MQ=255)

                                                                aTCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCt                             >  2:2879556/1‑100 (MQ=255)

                                                                aTCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCt                             >  2:1015789/1‑100 (MQ=255)

                                                                 tCCTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTg                            <  2:202912/100‑1 (MQ=255)

                                                                   cTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGa                          <  2:3258685/100‑1 (MQ=255)

                                                                   cTTCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGa                          <  2:2898988/100‑1 (MQ=255)

                                                                    ttCTCAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGAc                         >  1:1209033/1‑100 (MQ=255)

                                                                        cAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTg                     >  2:1751530/1‑100 (MQ=255)

                                                                        cAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTg                     >  2:1130143/1‑100 (MQ=255)

                                                                        cAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTg                     >  1:1763646/1‑100 (MQ=255)

                                                                        cAACTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTg                     <  2:621192/100‑1 (MQ=255)

                                                                         aaCTTAAAGACTAATACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTgg                    <  2:2312371/100‑1 (MQ=255)

                                                                         aaCTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTgg                    <  2:1209030/100‑1 (MQ=255)

                                                                         aaCTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTgg                    <  2:318802/100‑1 (MQ=255)

                                                                           cTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCa                  <  2:2187987/100‑1 (MQ=255)

                                                                           cTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCa                  <  2:734071/100‑1 (MQ=255)

                                                                           cTTAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCa                  <  1:1838952/100‑1 (MQ=255)

                                                                            tcatgacagtagactGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCAt                 >  2:3318596/11‑100 (MQ=255)

                                                                             tAAAGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTACACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCAt                 >  2:2098096/1‑99 (MQ=255)

                                                                              aaaGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGa               <  1:2887161/100‑1 (MQ=255)

                                                                              aaaGACTAAGACTGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGa               >  2:426065/1‑100 (MQ=255)

                                                                                 gactaagactGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACg            >  1:2031216/1‑100 (MQ=255)

                                                                                 gactaagactGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACg            >  1:2131155/1‑100 (MQ=255)

                                                                                 acagtagactGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACg            <  1:3318609/95‑1 (MQ=255)

                                                                                  actaagactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACg            >  2:1446025/1‑100 (MQ=255)

                                                                                   ctaagactGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGtt          >  1:685496/1‑100 (MQ=255)

                                                                                   ctaagactGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGtt          <  1:2270757/100‑1 (MQ=255)

                                                                                     aagactGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAt        <  2:313655/100‑1 (MQ=255)

                                                                                     aagactGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAt        >  2:2632136/1‑100 (MQ=255)

                                                                                     aagactGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAt        <  1:2537177/100‑1 (MQ=255)

                                                                                     aagactGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAt        >  1:3124406/1‑100 (MQ=255)

                                                                                     aagactGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAt        >  1:3253154/1‑100 (MQ=255)

                                                                                        actGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCg     <  1:101108/100‑1 (MQ=255)

                                                                                         ctGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGt    >  1:556366/1‑100 (MQ=255)

                                                                                          tGTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTc   <  1:1015790/100‑1 (MQ=255)

                                                                                           gTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCt  >  2:527741/1‑100 (MQ=255)

                                                                                           gTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCt  >  1:2563149/1‑100 (MQ=255)

                                                                                           gTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCt  <  2:2761283/100‑1 (MQ=255)

                                                                                           gTCATG‑AAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCt  <  2:2631354/100‑1 (MQ=255)

CACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCT  >  NC_000913/1755604‑1755795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`