Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 432,193 +G intergenic (‑180/+119) tsx ← / ← yajI nucleoside channel, receptor of phage T6 and colicin K/putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913432,1891.G96.0% 539.4 / 13.2 151intergenic (‑176/+123)tsx/yajInucleoside channel, receptor of phage T6 and colicin K/putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base . (2/4);  new base G (68/77);  total (70/81)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

AGGAGCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCG  >  NC_000913/432090‑432283
                                                                                                    |                                                                                                
tccgatcTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTcgggc                                                                                                   <  1:3034252/94‑1 (MQ=255)
aggagCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTcgggc                                                                                                   <  1:817390/100‑1 (MQ=255)
   agCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTcgggcggn                                                                                               <  2:401883/100‑2 (MQ=255)
    gCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCgggg                                                                                             <  2:2982176/100‑1 (MQ=255)
    gCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCgggg                                                                                             <  2:2478472/100‑1 (MQ=255)
    atcTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCgggg                                                                                             <  1:129316/98‑1 (MQ=255)
      cTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCggggg                                                                                            >  1:2451529/1‑98 (MQ=255)
      cTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGAt                                                                                          >  1:2028771/1‑100 (MQ=255)
       tattatAAAAATCATTGGTAACAAAAATATTCTTCGTTACACGGTTCTATCATTTACGTAATCGAGTGGGTTCAGCTTTACACACATTCGGGCgggg                                                                                             <  2:3216518/97‑1 (MQ=255)
       tattatAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCgggggat                                                                                          <  1:2668969/100‑4 (MQ=255)
       tattatAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCgggggat                                                                                          >  2:2998454/1‑97 (MQ=255)
        attatAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTcgggcagatcgga                                                                                           >  2:3034240/1‑92 (MQ=255)
        attatAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTcgggcgaaagtca                                                                                          >  1:3400201/1‑93 (MQ=255)
        attatAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCgggggatt                                                                                         >  1:211803/1‑96 (MQ=255)
         ttatAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCgggggattg                                                                                        <  2:98254/100‑6 (MQ=255)
           ataAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTa                                                                                      <  2:1213334/100‑1 (MQ=255)
            taAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTAc                                                                                     <  1:454439/100‑1 (MQ=255)
            taAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTAc                                                                                     >  1:653778/1‑100 (MQ=255)
            taAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAAGCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTAc                                                                                     <  2:2684082/100‑1 (MQ=255)
             aaaaaTCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACTTAATCGATTGCTTTCACGTTTACACACATTCGGACgggggattg                                                                                        >  2:1536803/1‑91 (MQ=255)
             aaaaaTCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACt                                                                                    <  1:2198213/100‑1 (MQ=255)
             aaaaaTCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACt                                                                                    >  2:3263772/1‑100 (MQ=255)
              ctcttccgatcTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTg                                                                                   <  1:1870172/90‑1 (MQ=255)
              aaaaTCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTg                                                                                   >  2:2320849/1‑100 (MQ=255)
                 aTCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACt                                                                                <  2:709891/100‑1 (MQ=255)
                  tCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACtt                                                                               >  1:3139285/1‑100 (MQ=255)
                    aTTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTc                                                                             <  2:2065856/100‑1 (MQ=255)
                     ttGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCg                                                                            <  1:127153/100‑1 (MQ=255)
                       gCTCACAAAAATATGTTTCGTTTCACAGTTCTATTATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGcc                                                                          <  2:1794899/100‑1 (MQ=255)
                       gCTCACAAAAATATGTTTAGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGGCTTTCGc                                                                           >  1:631310/1‑99 (MQ=255)
                       gCTCACAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGccc                                                                         >  1:2063597/1‑100 (MQ=255)
                        cTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGccc                                                                         >  2:467977/1‑100 (MQ=255)
                         tCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCt                                                                        >  2:1587023/1‑100 (MQ=255)
                          cacaAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCtt                                                                       <  1:2998466/100‑1 (MQ=255)
                           acaAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCttg                                                                      >  2:2592855/1‑100 (MQ=255)
                            caAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGCTTGTACTGACTTTCGCCCttgt                                                                     <  2:1977515/100‑1 (MQ=255)
                             aaaaaTATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCttgtt                                                                    <  1:1750820/100‑1 (MQ=255)
                              aaaaTATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCttgttg                                                                   <  2:3349392/100‑1 (MQ=255)
                                 atatGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCaa                                                                <  1:3217652/100‑1 (MQ=255)
                                   atGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAAc                                                              >  1:1497160/1‑100 (MQ=255)
                                    tGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACt                                                             >  2:326990/1‑100 (MQ=255)
                                     gtttcgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACtt                                                            >  2:2226792/1‑100 (MQ=255)
                                     gtttcgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACtt                                                            >  2:45093/1‑100 (MQ=255)
                                     gtttcgtttcACAGTTCTATCAGTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACtt                                                            >  2:1980706/1‑100 (MQ=255)
                                       ttcgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACtttt                                                          <  2:3356103/100‑1 (MQ=255)
                                       ttcgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACtttt                                                          <  2:1497157/100‑1 (MQ=255)
                                       ttcgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACtttt                                                          <  2:2451521/100‑1 (MQ=255)
                                        tccgatctcaGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTa                                                         <  1:1652116/92‑1 (MQ=255)
                                         cttgtcACATTTCGAGCAGTGACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTAc                                                        <  1:2327337/96‑1 (MQ=255)
                                         cgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTAc                                                        <  1:2468184/100‑1 (MQ=255)
                                         cgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTAc                                                        >  2:108903/1‑100 (MQ=255)
                                         cgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTAc                                                        <  1:1002390/100‑1 (MQ=255)
                                          gtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACt                                                       <  2:3139273/100‑1 (MQ=255)
                                          gtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACt                                                       >  1:3330303/1‑100 (MQ=255)
                                           tttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTa                                                      <  2:779952/100‑1 (MQ=255)
                                            ttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTAt                                                     >  1:1635821/1‑100 (MQ=255)
                                            ttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTAt                                                     >  1:2565975/1‑100 (MQ=255)
                                             tcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATc                                                    >  1:408087/1‑100 (MQ=255)
                                             tcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATc                                                    <  1:212832/100‑1 (MQ=255)
                                             tcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGTATTGTACTGACTTTCGCCCTGGTTGCAAACTTTTACTATc                                                    <  2:373696/100‑1 (MQ=255)
                                              cacaGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCa                                                   <  2:3152520/100‑1 (MQ=255)
                                              cacaGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCa                                                   >  2:212831/1‑100 (MQ=255)
                                                caGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAAt                                                 >  2:2717317/1‑100 (MQ=255)
                                                  gTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATcc                                               <  2:2937398/100‑1 (MQ=255)
                                                    tCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAg                                             >  2:125317/1‑100 (MQ=255)
                                                    tCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAg                                             <  1:1271607/100‑1 (MQ=255)
                                                      tATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGt                                           <  1:26416/100‑1 (MQ=255)
                                                          aTTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGATTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATg                                       >  2:2396463/1‑100 (MQ=255)
                                                          aTTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATg                                       >  1:287749/1‑100 (MQ=255)
                                                            ttACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACCATCAATCCAGGTGATGt                                      <  2:2832984/99‑1 (MQ=255)
                                                             tACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTaa                                    <  2:1046139/100‑1 (MQ=255)
                                                             tACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTaa                                    >  1:836556/1‑100 (MQ=255)
                                                             tACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTa                                     >  1:1174148/1‑99 (MQ=255)
                                                              aCGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAg                                   <  2:1319557/100‑1 (MQ=255)
                                                                gTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAg                                   >  2:636715/1‑98 (MQ=255)
                                                                gTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCa                                 >  1:1112455/1‑100 (MQ=255)
                                                                 tAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCag                                <  1:326991/100‑1 (MQ=255)
                                                                   aTCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCagag                              >  1:961926/1‑100 (MQ=255)
                                                                    tCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGt                             <  2:184652/100‑1 (MQ=255)
                                                                      gATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTaa                           <  2:418546/100‑1 (MQ=255)
                                                                      gATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTaa                           <  2:836556/100‑1 (MQ=255)
                                                                       aTTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAAc                          >  2:488071/1‑100 (MQ=255)
                                                                        ttGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACa                         <  1:2820702/100‑1 (MQ=255)
                                                                            gTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAAt                     >  1:1553112/1‑100 (MQ=255)
                                                                            gTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAAt                     >  2:767364/1‑100 (MQ=255)
                                                                               cACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGAc                  >  1:2557486/1‑100 (MQ=255)
                                                                               cACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGAc                  <  2:2127826/100‑1 (MQ=255)
                                                                               cACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGAc                  <  2:2559037/100‑1 (MQ=255)
                                                                                 cGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACgg                <  1:467978/100‑1 (MQ=255)
                                                                                     tACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGaa            >  2:206123/1‑100 (MQ=255)
                                                                                       cacacaTTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAg          >  2:34301/1‑100 (MQ=255)
                                                                                       cacacaTTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAg          >  2:245310/1‑100 (MQ=255)
                                                                                        ncacaTTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGa         >  2:186013/2‑100 (MQ=255)
                                                                                        acacaTTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGa         >  1:1969995/1‑100 (MQ=255)
                                                                                        acacaTTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGa         <  1:1065575/100‑1 (MQ=255)
                                                                                         cacaTTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGAt        >  1:2347249/1‑100 (MQ=255)
                                                                                          acaTTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATg       <  1:2320856/100‑1 (MQ=255)
                                                                                           caTTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCATTCCCGATGATGTAAGAAGAGTAACAAAATGACGGGGAAAGATGt      >  2:1286950/1‑100 (MQ=255)
                                                                                           caTTCGGGCGGGTGATTGTACTGACTGTCGCGCTTGTTGCAAACGTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATg       <  2:3090312/99‑1 (MQ=255)
                                                                                               cgggcgggGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCg  <  2:2790088/100‑1 (MQ=255)
                                                                                                    |                                                                                                
AGGAGCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCG  >  NC_000913/432090‑432283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: