Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,866,592 +C coding (960/993 nt) rpoS ← RNA polymerase, sigma S (sigma 38) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,866,5881.C98.4% 459.0 / 0.4 124coding (964/993 nt)rpoSRNA polymerase, sigma S (sigma 38) factor
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base C (65/57);  total (65/59)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 131 total aligned reads displayed.

CATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAG‑CCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACA  >  NC_000913/2866489‑2866682
                                                                                                    |                                                                                              
cATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAg                                                                                                 <  2:1355547/100‑1 (MQ=255)
cATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAg                                                                                                 <  1:3006679/100‑1 (MQ=255)
    ggACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAG‑cccc                                                                                            >  1:2792041/1‑100 (MQ=255)
     gACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAG‑ccccc                                                                                           <  2:1493744/100‑2 (MQ=255)
        cTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCCTACTTACTCGCGAACCAGCGCTTCGATATTCAG‑ccccctcc                                                                                        >  2:1118675/1‑96 (MQ=255)
           ttATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGtt                                                                                      <  2:2657263/100‑1 (MQ=255)
            tATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGttt                                                                                     >  1:1591624/1‑100 (MQ=255)
             aTTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTg                                                                                    <  2:360703/100‑1 (MQ=255)
               tgtgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCa                                                                                  <  1:3260484/100‑1 (MQ=255)
               tgtgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAG‑CCCCTGCGTTTGCAg                                                                                 <  2:718272/100‑1 (MQ=255)
                gtgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAg                                                                                 <  2:2091804/100‑1 (MQ=255)
                gtgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAg                                                                                 <  1:1975886/100‑1 (MQ=255)
                gtgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAg                                                                                 <  2:2995730/100‑1 (MQ=255)
                gtgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAg                                                                                 >  1:2576182/1‑100 (MQ=255)
                 tgCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAgg                                                                                <  1:517785/100‑1 (MQ=255)
                  gCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGa                                                                               >  2:3113611/1‑100 (MQ=255)
                   cacaGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGAt                                                                              >  1:2586945/1‑100 (MQ=255)
                    acaGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGAtt                                                                             >  2:37837/1‑100 (MQ=255)
                    acaGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGAtt                                                                             >  1:2314947/1‑100 (MQ=255)
                      aGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTc                                                                           >  2:3145277/1‑100 (MQ=255)
                       gAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAgatcggaa                                                                          >  1:294923/1‑93 (MQ=255)
                        aaaaGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTcgc                                                                         >  2:2532793/1‑100 (MQ=255)
                        aaaaGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTcgc                                                                         <  2:822352/100‑1 (MQ=255)
                         aaaGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTcgcg                                                                        <  1:2114800/100‑1 (MQ=255)
                         aaaGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTcgcg                                                                        >  1:3069436/1‑100 (MQ=255)
                           aGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCa                                                                      >  1:1098440/1‑100 (MQ=255)
                           aGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCa                                                                      <  1:362228/100‑1 (MQ=255)
                            ggCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCaa                                                                     >  2:3264549/1‑100 (MQ=255)
                            ggCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCaa                                                                     <  1:1585253/100‑1 (MQ=255)
                               cAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACg                                                                  <  2:1043205/100‑1 (MQ=255)
                                   cTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGgcgc                                                              >  1:2093394/1‑100 (MQ=255)
                                   cTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGgcgc                                                              <  1:2532801/100‑1 (MQ=255)
                                   cTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGgcgc                                                              <  2:2586937/100‑1 (MQ=255)
                                    tCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCa                                                             <  1:1267477/100‑1 (MQ=255)
                                     cGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAg                                                            >  1:2932024/1‑100 (MQ=255)
                                     cGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAg                                                            >  1:1118775/1‑100 (MQ=255)
                                     cGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAg                                                            >  2:1752681/1‑100 (MQ=255)
                                      gCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAgg                                                           >  2:3177069/1‑100 (MQ=255)
                                       cTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGc                                                          <  1:992899/100‑1 (MQ=255)
                                        ttGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGcc                                                         <  1:1945111/100‑1 (MQ=255)
                                          gagaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCtt                                                       <  1:1285991/100‑1 (MQ=255)
                                           agaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTc                                                      >  1:1644130/1‑100 (MQ=255)
                                            gaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCa                                                     >  2:1991321/1‑100 (MQ=255)
                                            gaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCa                                                     <  2:1644128/100‑1 (MQ=255)
                                            gaCTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCGTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCa                                                     <  2:327799/100‑1 (MQ=255)
                                               tGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAAcc                                                  >  1:3148753/1‑100 (MQ=255)
                                               tGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAAcc                                                  <  1:3145289/100‑1 (MQ=255)
                                               tGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAAcc                                                  <  1:2902488/100‑1 (MQ=255)
                                                ggCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCt                                                 >  1:2970335/1‑100 (MQ=255)
                                                  ccTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGa                                               <  1:1953959/100‑1 (MQ=255)
                                                     ttCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATc                                            >  2:2436041/1‑100 (MQ=255)
                                                     ttCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATc                                            <  1:940913/100‑1 (MQ=255)
                                                       cTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTg                                          >  2:1193721/1‑100 (MQ=255)
                                                       cTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTg                                          >  1:796475/1‑100 (MQ=255)
                                                        tGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTgg                                         >  1:1819414/1‑100 (MQ=255)
                                                         gACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGc                                        >  2:1790570/1‑100 (MQ=255)
                                                         gACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGc                                        >  2:279848/1‑100 (MQ=255)
                                                         gACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGc                                        >  2:2766653/1‑100 (MQ=255)
                                                          aCAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCg                                       <  1:508476/100‑1 (MQ=255)
                                                          aCAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCg                                       <  2:1937483/100‑1 (MQ=255)
                                                           cAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGc                                        >  2:2276280/1‑98 (MQ=255)
                                                            aGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGaa                                     <  2:2188790/100‑1 (MQ=255)
                                                            aGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGaa                                     >  2:2201275/1‑100 (MQ=255)
                                                            aGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAG‑CCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAac                                    <  2:2584269/100‑1 (MQ=255)
                                                             gATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAac                                    <  2:2690604/100‑1 (MQ=255)
                                                                gCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACg                                 >  2:2912864/1‑100 (MQ=255)
                                                                gCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGAGTTCGCGCAAACGGCGCGGGCCTTCAACCTGAATCTGGCGAACACg                                 <  1:992202/100‑1 (MQ=255)
                                                                  ttacttacTCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGtt                               <  1:37837/100‑1 (MQ=255)
                                                                     cttactCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCAc                            >  1:1579661/1‑100 (MQ=255)
                                                                     cttactCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCAc                            >  2:1094448/1‑100 (MQ=255)
                                                                       tactCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACgg                          <  1:670653/100‑1 (MQ=255)
                                                                       tactCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACgg                          >  1:1084505/1‑100 (MQ=255)
                                                                       tactCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACgg                          >  1:34152/1‑100 (MQ=255)
                                                                        actCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACggg                         <  1:109828/100‑1 (MQ=255)
                                                                        actCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACggg                         <  2:1000497/100‑1 (MQ=255)
                                                                        actCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACgg                          <  1:2394639/99‑1 (MQ=255)
                                                                         ctCGCGGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGt                        >  1:2759914/1‑100 (MQ=255)
                                                                           cgcgGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAGCACGTTCACGGGTGa                      >  2:3009348/1‑100 (MQ=255)
                                                                           cgcgGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGa                      >  1:1459912/1‑100 (MQ=255)
                                                                           cgcgGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGa                      >  1:57674/1‑100 (MQ=255)
                                                                           cgcgGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGa                      >  2:1563849/1‑100 (MQ=255)
                                                                           cgcgGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGa                      >  2:2552445/1‑100 (MQ=255)
                                                                           cgcgGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGa                      >  1:136130/1‑100 (MQ=255)
                                                                           cgccGAACAGCCCTTCGATATTCAGCCCCCTGCGTTTGCACGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTg                       <  2:3282214/99‑1 (MQ=255)
                                                                             cgGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAgg                    <  2:2500962/100‑1 (MQ=255)
                                                                             cgGAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAgg                    <  2:796475/100‑1 (MQ=255)
                                                                               gAACAGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGcc                  >  1:909476/1‑100 (MQ=255)
                                                                                aaCAGCGCTTCGATACTCAGCCCCCTGCGTTTGCAGGATTTCGCTCAACCCGCGCAGGCCTTCAACCTCAATCTTGCGAACACGTTCACGGGTGAGGc                   <  2:3298361/98‑1 (MQ=255)
                                                                                   aGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAAtt              >  1:206793/1‑100 (MQ=255)
                                                                                   aGCGCTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAAtt              >  1:2882007/1‑100 (MQ=255)
                                                                                      gcTTCGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCa           >  2:2878246/1‑100 (MQ=255)
                                                                                          cGATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGAc       <  2:2759903/100‑1 (MQ=255)
                                                                                           gATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGAcc      <  2:3351394/100‑1 (MQ=255)
                                                                                           gATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGAcc      <  2:2997340/100‑1 (MQ=255)
                                                                                           gATATTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGAcc      >  1:494452/1‑100 (MQ=255)
                                                                                            atatTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCt     <  1:3113623/100‑1 (MQ=255)
                                                                                            atatTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCt     >  1:947930/1‑100 (MQ=255)
                                                                                             tatTCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTa    >  1:1972807/1‑100 (MQ=255)
                                                                                               ttCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACa  <  1:2773967/100‑1 (MQ=255)
                                                                                               ttCAGCCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACa  <  1:736864/100‑1 (MQ=255)
                                                                                                    |                                                                                              
CATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAG‑CCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAACCTGAATCTGGCGAACACGTTCACGGGTGAGGCCAATTTCACGACCTACA  >  NC_000913/2866489‑2866682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑