BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,379,266	(C)_7→8	coding (732/1128 nt)	zapE ←	divisome ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,379,259	1	.	C	95.2%	436.5 / 16.2	125	coding (739/1128 nt)	zapE	divisome ATPase
Reads supporting (aligned to +/- strand): ref base . (5/1); new base C (59/60); total (64/61)
Fisher's exact test for biased strand distribution p-value = 2.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 141 total aligned reads displayed.

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGC  >  NC_000913/3379160‑3379353

cGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑Gttt                                                                                                    <  1:2989553/100‑1 (MQ=255)

cGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑Gttt                                                                                                    >  1:3108238/1‑100 (MQ=255)

  cacaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑cc                                                                                                <  2:92078/100‑1 (MQ=255)

  cacaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑cc                                                                                                >  1:504971/1‑100 (MQ=255)

        gtagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATCGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑ct‑cc‑cccc                                                                                        <  1:2939124/100‑2 (MQ=255)

         tagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑cc‑cc‑cccc                                                                                        >  2:329050/1‑98 (MQ=255)

          agtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑cc‑cc‑ccccgc                                                                                      >  1:2423210/1‑97 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑cc‑cc‑ccccgcc                                                                                     >  2:313689/1‑96 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑cc‑cc‑ccccgcc                                                                                     >  1:1153492/1‑96 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑cc‑cc‑ccccgcc                                                                                     >  2:1002451/1‑96 (MQ=255)

             aaaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAg                                                                                    >  1:2736712/1‑100 (MQ=255)

             aaaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAg                                                                                    >  1:1400453/1‑100 (MQ=255)

              aaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAgc                                                                                   <  2:1683250/100‑1 (MQ=255)

               aaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAgcg                                                                                  <  1:310179/100‑1 (MQ=255)

                agagACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAgcgc                                                                                 <  2:1497387/100‑1 (MQ=255)

                agagACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAgcgc                                                                                 >  1:2732445/1‑100 (MQ=255)

                 gagaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGcc                                                                                >  1:2669704/1‑100 (MQ=255)

                 gagaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGcc                                                                                >  2:73841/1‑100 (MQ=255)

                 gagaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGcc                                                                                >  2:73746/1‑100 (MQ=255)

                   gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCaa                                                                              >  2:1552801/1‑100 (MQ=255)

                    aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAAc                                                                             >  1:638587/1‑100 (MQ=255)

                    aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAAc                                                                             <  2:2736702/100‑1 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAAcc                                                                            >  2:120517/1‑100 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAAcc                                                                            <  2:1355004/100‑1 (MQ=255)

                       gCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCAt                                                                          >  1:2276996/1‑100 (MQ=255)

                         cAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAg                                                                        <  1:73745/100‑1 (MQ=255)

                         cAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAg                                                                        <  1:73840/100‑1 (MQ=255)

                              tCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTAt                                                                   <  1:2387717/100‑1 (MQ=255)

                                tGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATcc                                                                 <  1:3386897/100‑1 (MQ=255)

                                ccgatctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATcc                                                                 <  2:1207202/96‑1 (MQ=255)

                                 ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCa                                                                >  2:2721772/1‑100 (MQ=255)

                                  gTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGGTTC‑‑CC‑CC‑CCCGCCAGCCCCAACCATAGTTTAGCCAt                                                               >  2:3178583/1‑100 (MQ=255)

                                    tctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCAttt                                                             <  1:1479223/100‑1 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTg                                                            <  2:613131/100‑1 (MQ=255)

                                     cgcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTg                                                            <  2:349716/98‑1 (MQ=255)

                                      tcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTgc                                                           <  1:2502150/100‑1 (MQ=255)

                                       cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCagatcgg                                                          >  1:1207205/1‑94 (MQ=255)

                                         aCGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGcc                                                        <  1:267705/100‑1 (MQ=255)

                                             ccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCggg                                                    >  2:535603/1‑100 (MQ=255)

                                              cATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGt                                                   >  1:1960065/1‑100 (MQ=255)

                                               aTTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGtt                                                  >  2:2309767/1‑100 (MQ=255)

                                               aTTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGtt                                                  >  2:2306536/1‑100 (MQ=255)

                                               aTTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGtt                                                  >  2:1989065/1‑100 (MQ=255)

                                                ttgttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGttt                                                 <  1:1111129/100‑1 (MQ=255)

                                                 cttccgatctGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTc                                                <  2:1721775/91‑1 (MQ=255)

                                                  gttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCa                                               <  1:2549941/100‑1 (MQ=255)

                                                    tgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATc                                             >  1:933584/1‑100 (MQ=255)

                                                    tgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATc                                             >  1:3086928/1‑100 (MQ=255)

                                                    tgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATc                                             <  1:1421479/100‑1 (MQ=255)

                                                     gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATc                                             >  2:2945621/1‑99 (MQ=255)

                                                     gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCg                                            >  1:658889/1‑100 (MQ=255)

                                                     gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCg                                            <  2:1400125/100‑1 (MQ=255)

                                                     gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCg                                            >  2:497353/1‑100 (MQ=255)

                                                       tAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTg                                          >  2:2002322/1‑100 (MQ=255)

                                                         aTGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGaa                                        <  2:3097989/100‑1 (MQ=255)

                                                          tGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAg                                       <  1:1989069/100‑1 (MQ=255)

                                                           ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCagatcggaag                                      >  1:1721778/1‑91 (MQ=255)

                                                           ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGt                                      >  1:2742415/1‑100 (MQ=255)

                                                           ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑CC‑CCTCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAg                                       >  2:3293359/1‑99 (MQ=255)

                                                            gCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                     <  2:2586528/100‑1 (MQ=255)

                                                            gCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                     <  2:1793606/100‑1 (MQ=255)

                                                            gCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                     <  2:871678/100‑1 (MQ=255)

                                                            gCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACAATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                     >  2:794068/1‑100 (MQ=255)

                                                             ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                    >  2:3306931/1‑100 (MQ=255)

                                                             ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                    >  1:1717225/1‑100 (MQ=255)

                                                              cgctGGTTGCTTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑CCTCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGGGAAGTGGc                                   >  2:2329130/4‑100 (MQ=255)

                                                               tcttccgatcttCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                  <  2:1874278/90‑1 (MQ=255)

                                                               gATGGTTGATTTCTACCGTCGGTGAATTTTCTC‑CTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                  <  2:743073/100‑1 (MQ=255)

                                                                 tGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCgta                                <  1:2306542/100‑3 (MQ=255)

                                                                  ggTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaaa                               >  2:454594/1‑100 (MQ=255)

                                                                   gTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGagat                              >  2:2428825/1‑97 (MQ=255)

                                                                   gTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAg                              >  1:3187181/1‑100 (MQ=255)

                                                                   gTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAg                              <  1:780981/100‑1 (MQ=255)

                                                                   gTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAg                              <  1:780960/100‑1 (MQ=255)

                                                                   gTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAg                              >  2:2050596/1‑100 (MQ=255)

                                                                    ttGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGc                             <  2:834767/100‑1 (MQ=255)

                                                                     tGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGGGAAAGcc                            >  2:2911686/1‑100 (MQ=255)

                                                                      gATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCca                           >  2:380614/1‑100 (MQ=255)

                                                                        tttCTAACGTCGGTGAATTTTCTC‑GTTTCCCC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcac                          >  2:3294360/1‑100 (MQ=255)

                                                                        tttCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcaca                         <  2:251898/100‑1 (MQ=255)

                                                                          tCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGa                       >  1:476185/1‑100 (MQ=255)

                                                                          tCTAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGa                       >  1:2542529/1‑100 (MQ=255)

                                                                            tAACGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATg                     >  2:2424755/1‑100 (MQ=255)

                                                                             aaCGTCGGTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgc                    >  1:2169166/1‑100 (MQ=255)

                                                                              gatctggTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcg                   <  2:1949846/95‑1 (MQ=255)

                                                                                  cGGTGAAGTTTCTC‑TTGTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtg               <  1:3178595/100‑1 (MQ=255)

                                                                                   ggTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcgagatc              >  1:1949850/1‑95 (MQ=255)

                                                                                   ggTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtga              <  1:864067/100‑1 (MQ=255)

                                                                                   ggTGAATTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtga              >  1:2556488/1‑100 (MQ=255)

                                                                                      gatctttCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtg           <  1:1930900/96‑1 (MQ=255)

                                                                                        aTTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATCGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         >  1:2464431/1‑100 (MQ=255)

                                                                                        aTTTTCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         <  1:228743/100‑1 (MQ=255)

                                                                                          tttCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGagtgagtg       <  2:2043956/100‑1 (MQ=255)

                                                                                           ttCTC‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgagatc      >  2:1930896/1‑97 (MQ=255)

                                                                                           ttCTC‑GTTT‑‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTa     <  1:1042269/100‑1 (MQ=255)

                                                                                             ctc‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTAc    >  2:3014264/1‑100 (MQ=255)

                                                                                             ctc‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTAc    >  2:2894556/1‑100 (MQ=255)

                                                                                             ctc‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTAc    >  2:2556542/1‑100 (MQ=255)

                                                                                              tc‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACg   <  1:1258856/100‑1 (MQ=255)

                                                                                               c‑GTTTC‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc  >  2:1286659/1‑100 (MQ=255)

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑CC‑CC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGC  >  NC_000913/3379160‑3379353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`