BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,781,152	0	A	G	21.6%	199.8 / 25.7	102	intergenic (+135/‑63)	lldD/trmL	L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand): ref base A (53/27); new base G (0/22); total (53/49)
Fisher's exact test for biased strand distribution p-value = 4.16e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 104 total aligned reads displayed.

AGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGC  >  NC_000913/3781056‑3781251

agagGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAggg                                                                                                    <  1:2766826/100‑1 (MQ=255)

 gagGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAggg‑g                                                                                                  >  2:1547431/1‑100 (MQ=255)

 gagGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAggg‑g                                                                                                  <  2:32771/100‑1 (MQ=255)

     ggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑aaaa                                                                                             >  1:2189997/1‑100 (MQ=255)

     ggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑aaaa                                                                                             <  2:3184565/100‑1 (MQ=255)

      gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGCGAGGGCC‑AGGGTGAGGG‑G‑AAAAg                                                                                            >  1:2830124/1‑100 (MQ=255)

      gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAg                                                                                            <  1:3111586/100‑1 (MQ=255)

      gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAg                                                                                            >  2:2729489/1‑100 (MQ=255)

      gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAg                                                                                            >  1:1697859/1‑100 (MQ=255)

       tctgTTCGGCGCTGTATGTACTCCCTCACACCGACACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑ATAAg                                                                                            <  1:587993/96‑1 (MQ=37)

        ccGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGc                                                                                          >  2:45768/1‑100 (MQ=255)

          gTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAggga                                                                                         >  2:587992/1‑97 (MQ=255)

            tCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCa                                                                                      >  2:833720/1‑100 (MQ=255)

              ggCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACt                                                                                    >  1:721934/1‑100 (MQ=255)

               gcgcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTg                                                                                   >  1:3189463/1‑100 (MQ=255)

                 gcTGTATTTCCCCCCCCCCCCTAAACCGACCCCGCACTC‑TTTTTTCCCC‑CCCCCCCCCCCGGGGGGGGGGCCGGGGGGGGGGG‑G‑AAAAGGCCGc                                                                                       <  2:1222325/94‑1 (MQ=255)

                 gcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCt                                                                                 >  1:1410975/1‑100 (MQ=255)

                  cTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAACGGCCGCACTGCTc                                                                                >  2:1953272/1‑100 (MQ=255)

                   tgtatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCa                                                                               >  2:1369640/1‑100 (MQ=255)

                      ttccgatctcTCACTCGGAAACGACCCCGCACTCTTTTTTTCTCC‑CGCGCCCCCCCGGGGGGGGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTc                                                                                <  2:2300780/87‑1 (MQ=255)

                      atgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCa                                                                            >  1:1705791/1‑100 (MQ=255)

                      atgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCa                                                                            >  1:1706318/1‑100 (MQ=255)

                        gtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAAt                                                                          >  2:1864433/1‑100 (MQ=255)

                              ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTg                                                                    >  2:1347117/1‑100 (MQ=255)

                              ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTg                                                                    >  1:3183117/1‑100 (MQ=255)

                               ccccctctAAAACGACCCCCCCCTCTTTTTTCCCCC‑CCCCCCCCCCCGGGGAGGGGGCG‑GGGGGGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGc                                                                   <  1:480527/96‑1 (MQ=255)

                               cTCCCTCTAAAGCGACACCGCACTCTTTTTTTCCCC‑CCCGCCCCCCCGGGGAGAGGCCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGc                                                                   <  2:1326902/100‑1 (MQ=255)

                               cTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑CAAAGGCCGCCCTGCTCACCAATTTTCTGc                                                                   >  2:3077151/1‑100 (MQ=255)

                                tCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCt                                                                  >  1:2265495/1‑100 (MQ=255)

                                 cACTCTAAAACGACACCGCACTCTTTTTTTCGCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑A‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTa                                                                 <  2:1068236/100‑1 (MQ=255)

                                    tctGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCTGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                              <  1:2163769/100‑1 (MQ=255)

                                     ctGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                             >  1:2005585/1‑100 (MQ=255)

                                     ctGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                             >  1:1336380/1‑100 (MQ=255)

                                         aaCGACCCCGCCCTCTTTTTTCCCCC‑CTCGCCCCCCGGGGGAGGGGCCG‑GGGGTGGGGG‑A‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccc                                                         <  2:2490489/100‑1 (MQ=255)

                                           cGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc                                                       >  2:1934883/1‑100 (MQ=255)

                                           cGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc                                                       >  1:1333874/1‑100 (MQ=255)

                                            gACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                                                      >  1:2461463/1‑100 (MQ=255)

                                            gACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                                                      >  1:2705697/1‑100 (MQ=255)

                                                cgccACTCTTTTTTTCTCC‑CGCCCCCGCCGGGGGGGGGGGCC‑GGGGGGGGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                              <  2:1240662/85‑1 (MQ=255)

                                                ccGCCCTTTTTTTTTCCCC‑CCCGCCCCCCGGGGGGGGGGGCG‑GGGGGGGGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCAc                                                   <  2:1336377/99‑1 (MQ=255)

                                                ccGCCCTCTTTTTTTCCCC‑CTCGCCCCCCGGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCAc                                                   <  2:2265489/99‑1 (MQ=38)

                                                   cgctctTTTTTTCCCC‑CTCTCCCCCCCGGGGAGATGCCG‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcc                                                          <  2:1306190/87‑1 (MQ=255)

                                                   cACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAg                                               >  1:1892018/1‑100 (MQ=255)

                                                    ccgttttttgtCTCCGCTCCCCCCCCGGAGGGGAGGGCG‑GGGGTGGGGG‑A‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGTCCCCGCAc                                                   <  1:2175069/92‑1 (MQ=255)

                                                    ccctcttttttCCCC‑CCCCCCCCTCCGGGGGGGGGGGC‑GGGGGGGGGG‑GAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTaa                                                <  1:3322404/94‑1 (MQ=255)

                                                      tctTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                            >  2:2793058/1‑100 (MQ=255)

                                                       ctTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                           >  2:1150833/1‑100 (MQ=255)

                                                       ctTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                           >  2:3149337/1‑100 (MQ=255)

                                                        tttttttCTCC‑CTCGCCCCCCCGGGGAGGGGGCG‑GGGGGGAGGG‑A‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggg                                             <  2:3064999/97‑1 (MQ=255)

                                                        tttttttCTCC‑CCCCCCCCGCCGGGGGGGGGGCC‑GGGGGGGGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc                                          <  1:2466641/100‑1 (MQ=255)

                                                        tttttttC‑CC‑CCCCCCCCCCCGGGGGGGGGGCCGGGGGTGAGGG‑A‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggg                                             <  2:3189450/97‑1 (MQ=255)

                                                         ttttttCCCC‑CGGGCCCCCCCGGGGGGGGGGCG‑GGGGGGGGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc                                          <  2:2791790/99‑1 (MQ=255)

                                                         ttttttCACC‑CAACCCCCCCGGGAGAGGCGCCC‑GGGGTGGGGG‑A‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcc                                                          <  2:2203588/83‑1 (MQ=255)

                                                          tttttCCCC‑CCCCCCCCCCGGGGGGGGGGGCG‑GGGGGGGGGG‑A‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAg                                        <  1:435293/100‑1 (MQ=255)

                                                           ttttCCCC‑CCCCCCCCGCGGGGGGGGGGGCG‑GGGGTGGGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt                                       <  1:1876722/100‑1 (MQ=255)

                                                            tttCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTa                                      >  1:1704695/1‑100 (MQ=255)

                                                            tttCTCC‑CTCCCCCTTCGGGGGAGGGGGCG‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTa                                      <  1:1369643/100‑1 (MQ=255)

                                                             ttCTCC‑CCCGCCCCCCGGGGGGGGGGGCG‑GGGGGGGGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAt                                     <  1:1101063/100‑1 (MQ=255)

                                                             ttCCCC‑CCCGCCCCCCCGGGGAGGGGCCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTa                                      <  2:1410972/99‑1 (MQ=255)

                                                             tccccc‑cTCCCCCCCGGGGGGGGGGGGCG‑GGGGGGGGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTa                                      <  1:3149349/95‑1 (MQ=255)

                                                              tctcC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggagatcggaag                                    >  2:736080/1‑90 (MQ=255)

                                                               ctcC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGc                                   >  2:582918/1‑100 (MQ=255)

                                                               cccc‑cTCGCCCCCCGGGGGAGGGGGCG‑GGGGTGGGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGc                                   <  1:1547434/98‑1 (MQ=255)

                                                                ccccccccccccccCGGGGGAGGGGCC‑GGGGGGGGGG‑GAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATg                                    <  1:2729498/92‑1 (MQ=255)

                                                                ccc‑cTCGCCCCCCGGGGGGGGGGGCG‑GGGGTGGGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAt                                     <  1:183707/96‑1 (MQ=255)

                                                                 cc‑cTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa                                 >  1:2577297/1‑100 (MQ=255)

                                                                 cc‑cTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa                                 >  1:33360/1‑100 (MQ=255)

                                                                 cc‑cTCGCCCCCCGGGGGAGGGGGCC‑GGGGGGGGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATg                                    <  2:1755970/97‑1 (MQ=255)

                                                                  c‑cTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa                                >  2:2577672/1‑100 (MQ=255)

                                                                    cTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaaa                               >  1:1721217/1‑100 (MQ=255)

                                                                    cTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaaa                               >  2:1721530/1‑100 (MQ=255)

                                                                     cgcccccTCCGGGGGGGAGGCC‑GGGGGGGGGGTT‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaaa                               <  1:2648896/97‑1 (MQ=255)

                                                                      cGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACa                             >  2:2865503/1‑100 (MQ=255)

                                                                        ccccggaggggCGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc                           >  2:845701/8‑100 (MQ=255)

                                                                         cccTCGGGGGGGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCg                          <  1:579783/100‑1 (MQ=255)

                                                                         cccTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCg                          >  1:676210/1‑100 (MQ=255)

                                                                          ccccgggggggggggCG‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCg                          <  1:294077/94‑1 (MQ=255)

                                                                           cTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                        >  1:607656/1‑100 (MQ=255)

                                                                           cTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGAACTAAGGGGGCAGTATGCTAAACATCGTa                        >  2:1361687/1‑100 (MQ=255)

                                                                            ccgggggggggggCG‑GGGGTGGGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTAc                       <  1:149017/97‑1 (MQ=255)

                                                                             cgggggAGAGGGCG‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTAc                       <  2:3058551/97‑1 (MQ=255)

                                                                             ccGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACt                      >  2:1576419/1‑100 (MQ=255)

                                                                               ggggAGGGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGt                         <  2:1892014/95‑1 (MQ=255)

                                                                               ggggAGGGGCCG‑GGGGGGGGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACttt                    <  1:350548/100‑1 (MQ=255)

                                                                               ggggAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACttt                    >  1:3071619/1‑100 (MQ=255)

                                                                                 atctagGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                  <  1:2384165/96‑1 (MQ=255)

                                                                                  gcgagGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg                 <  1:845701/98‑1 (MQ=255)

                                                                                     agGGGC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAc              <  2:360609/100‑1 (MQ=255)

                                                                                     agGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCTCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAcagat              >  2:2384158/1‑96 (MQ=255)

                                                                                      gggCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc             >  1:945493/1‑100 (MQ=255)

                                                                                      gggCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc             >  2:2260372/1‑100 (MQ=255)

                                                                                      gggCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc             >  2:1705406/1‑100 (MQ=255)

                                                                                       gccc‑GGGGTGGGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                  <  2:721934/92‑1 (MQ=255)

                                                                                       cgatctgggTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc             <  1:11406/94‑1 (MQ=255)

                                                                                        gCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCCGTAATTTGCTGCTATCCAGCCCCCGCGCCAGGGGGTCATTATGCTAAATAGCGTACCTTActcacga            >  2:203766/1‑93 (MQ=255)

                                                                                            gggggggggg‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGaa         <  1:2254904/99‑1 (MQ=255)

                                                                                             gggTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGatcg       >  2:11406/1‑97 (MQ=255)

                                                                                                 gAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTcc    <  2:1706316/99‑1 (MQ=255)

                                                                                                 gAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTcc    <  2:1705789/99‑1 (MQ=255)

                                                                                                  aGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGc  <  2:1721214/100‑1 (MQ=255)

AGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCC‑CTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGG‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGC  >  NC_000913/3781056‑3781251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`