Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 741,817 C→T G247D (GGC→GAC)  dtpD ← dipeptide and tripeptide permease D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913741,8170CT98.6% 493.2 / ‑4.4 138G247D (GGC→GAC) dtpDdipeptide and tripeptide permease D
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (62/74);  total (63/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

AACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACCAGCAGC  >  NC_000913/741721‑741912
                                                                                                |                                                                                               
aacaTACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCaa                                                                                              >  1:10678/1‑100 (MQ=255)
aacaTACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCaa                                                                                              >  2:1784918/1‑100 (MQ=255)
 acaTACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCaat                                                                                             >  2:3121389/1‑100 (MQ=255)
   aTACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCaataa                                                                                           <  2:2492909/100‑1 (MQ=255)
   aTACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCaataa                                                                                           <  2:628126/100‑1 (MQ=255)
   aTACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCaataa                                                                                           >  2:1731574/1‑100 (MQ=255)
   aTACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCaataa                                                                                           >  1:1880812/1‑100 (MQ=255)
    tACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCaataat                                                                                          >  2:2473688/1‑100 (MQ=255)
    tACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCaataat                                                                                          <  1:2859366/100‑1 (MQ=255)
    tACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCaataat                                                                                          >  2:260691/1‑100 (MQ=255)
      cTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAg                                                                                        >  1:1594760/1‑100 (MQ=255)
        gaagaaGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTc                                                                                      >  1:778912/1‑100 (MQ=255)
          agaagGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTcgc                                                                                    <  1:3279767/100‑1 (MQ=255)
           gaagGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTcgcg                                                                                   >  2:666396/1‑100 (MQ=255)
           gaagGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTcgcg                                                                                   <  1:2807373/100‑1 (MQ=255)
             agGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGAc                                                                                 >  2:491573/1‑100 (MQ=255)
              ggTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACa                                                                                <  1:2478622/100‑1 (MQ=255)
               gTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACaa                                                                               >  2:1502445/1‑100 (MQ=255)
               gTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACaa                                                                               >  2:2056502/1‑100 (MQ=255)
                tGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAAt                                                                              >  1:1668519/1‑100 (MQ=255)
                  aGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTa                                                                            <  1:882654/100‑1 (MQ=255)
                  aGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTa                                                                            <  2:1594757/100‑1 (MQ=255)
                     gTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAgg                                                                         >  2:2127180/1‑100 (MQ=255)
                      tCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGc                                                                        <  2:559517/100‑1 (MQ=255)
                      tCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGc                                                                        >  1:56756/1‑100 (MQ=255)
                        tcaATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGt                                                                      <  1:500965/99‑1 (MQ=255)
                        acaATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGt                                                                      >  1:1442662/1‑100 (MQ=255)
                         caATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGta                                                                     >  2:500964/1‑100 (MQ=255)
                         caATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGta                                                                     >  2:1037183/1‑100 (MQ=255)
                         caATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGta                                                                     <  2:635417/100‑1 (MQ=255)
                         caATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGta                                                                     <  2:1473235/100‑1 (MQ=255)
                          aaTCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCTAGTACACCCAAGTCAATAATAGTCGCGATAATTAAGGCGtct                                                                    <  1:2295185/100‑3 (MQ=255)
                           aTCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGtat                                                                    <  2:537698/99‑1 (MQ=255)
                            ctcttccgatcTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATAc                                                                  <  2:1949505/90‑1 (MQ=255)
                             cAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATAcc                                                                 <  2:925931/100‑1 (MQ=255)
                              aGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCg                                                                <  2:2703846/100‑1 (MQ=255)
                              aGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCg                                                                <  1:2803916/100‑1 (MQ=255)
                               gCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGa                                                               <  1:836709/100‑1 (MQ=255)
                               gCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAAGTCAATAATAGTCGCGACAATTAAGGCGTATACCGa                                                               >  1:2389718/1‑100 (MQ=255)
                                 tcttccgatctCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGAcc                                                             <  2:1248731/90‑1 (MQ=255)
                                 cccAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGAcc                                                             <  2:1945754/100‑1 (MQ=255)
                                 cccAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGAcc                                                             <  1:796384/100‑1 (MQ=255)
                                 cccAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGAcc                                                             <  1:2127185/100‑1 (MQ=255)
                                 cccAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGAcc                                                             <  1:2079481/100‑1 (MQ=255)
                                   cAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCAc                                                           <  1:1784922/100‑1 (MQ=255)
                                      cTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCActct                                                        <  2:1204021/100‑1 (MQ=255)
                                      cTCCTTCCGCCGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATGGTTGCGACAATTAAGGCGTATACCGACCActct                                                        <  2:2235063/100‑1 (MQ=255)
                                          ttCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTcc                                                    <  1:2683101/100‑1 (MQ=255)
                                           tCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCa                                                   >  2:236133/1‑100 (MQ=255)
                                           tCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCa                                                   >  1:1001345/1‑100 (MQ=255)
                                            ccGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCagatcggaaga                                                  >  1:1248734/1‑90 (MQ=255)
                                            ccGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAg                                                  <  2:2645918/100‑1 (MQ=255)
                                             cGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGa                                                 >  1:2662529/1‑100 (MQ=255)
                                             cGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGa                                                 >  2:300373/1‑100 (MQ=255)
                                              gCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGaa                                                >  1:1566384/1‑100 (MQ=255)
                                               cTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAAc                                               <  1:126233/100‑1 (MQ=255)
                                               cTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAAc                                               <  1:722785/100‑1 (MQ=255)
                                                tGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACa                                              <  2:1668517/100‑1 (MQ=255)
                                                 gTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAg                                             <  2:155684/100‑1 (MQ=255)
                                                 gTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAg                                             >  1:995330/1‑100 (MQ=255)
                                                   ttCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTa                                           >  1:471794/1‑100 (MQ=255)
                                                   ttCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTa                                           <  2:3370175/100‑1 (MQ=255)
                                                   ttCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTa                                           >  1:3271468/1‑100 (MQ=255)
                                                     cTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATg                                         >  1:3372161/1‑100 (MQ=255)
                                                        gTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTa                                      >  1:2544248/1‑100 (MQ=255)
                                                         tcttccgatctGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTaa                                     <  2:3352469/90‑1 (MQ=255)
                                                           ttCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATc                                   <  2:2544240/100‑1 (MQ=255)
                                                             cTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAg                                 <  1:1502448/100‑1 (MQ=255)
                                                             cTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAg                                 <  2:3322204/100‑1 (MQ=255)
                                                              tGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCACTAATAGTCGCGTCAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATc                                   >  1:2050250/1‑97 (MQ=255)
                                                               gCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATACTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTg                               <  2:2389711/100‑1 (MQ=255)
                                                                cTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGc                              <  2:2823344/100‑1 (MQ=255)
                                                                cTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGc                              <  2:2991212/100‑1 (MQ=255)
                                                                cTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGc                              <  2:1477465/100‑1 (MQ=255)
                                                                 tttGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCa                             <  1:668515/100‑1 (MQ=255)
                                                                   tGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAgg                           <  2:778912/100‑1 (MQ=255)
                                                                    gCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGc                          >  1:3405513/1‑100 (MQ=255)
                                                                        tAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTg                      <  1:2943159/100‑1 (MQ=255)
                                                                        tAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTg                      <  1:2056506/100‑1 (MQ=255)
                                                                        tAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTg                      <  1:2773066/100‑1 (MQ=255)
                                                                           aTTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGcca                   >  1:910651/1‑100 (MQ=255)
                                                                             ttttGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGccacc                 <  1:1037184/100‑1 (MQ=255)
                                                                               ttGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACcag               <  2:1291735/100‑1 (MQ=255)
                                                                                  ccAGTACACCCAGGTGAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACcagcag            <  1:3347063/100‑1 (MQ=255)
                                                                                  ccAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACcagcag            >  2:2009002/1‑100 (MQ=255)
                                                                                  ccAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACcagcag            <  2:103115/100‑1 (MQ=255)
                                                                                  ccAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACcagcag            >  2:377037/1‑100 (MQ=255)
                                                                                   cAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGa           >  2:1207586/1‑100 (MQ=255)
                                                                                   cAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGa           >  1:13715/1‑100 (MQ=255)
                                                                                     gTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAAc         >  2:337290/1‑100 (MQ=255)
                                                                                      tccacCCAGGTCAATAATAGTCGCGACAATTATGGCGTATACCGACCCCTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAAcc        <  1:1550587/98‑1 (MQ=255)
                                                                                       acacCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAAcc        <  1:2473696/99‑1 (MQ=255)
                                                                                        cacCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACcag      >  1:2577139/1‑100 (MQ=255)
                                                                                        cacCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACcag      >  2:121663/1‑100 (MQ=255)
                                                                                         acCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACcagc     >  1:1266558/1‑100 (MQ=255)
                                                                                          cccAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACcagca    <  1:1637440/100‑1 (MQ=255)
                                                                                           tcAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACcagcag   <  2:3328901/99‑1 (MQ=255)
                                                                                           ccAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACcagca  >  1:2894514/1‑99 (MQ=255)
                                                                                           ccAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACcagcag   <  2:2474397/100‑1 (MQ=255)
                                                                                            cAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACCagcagc  >  1:2295306/1‑100 (MQ=255)
                                                                                                |                                                                                               
AACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACCAGCAGC  >  NC_000913/741721‑741912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: