BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,602,117	A→G	I210V (ATT→GTT)	lsrA →	autoinducer 2 import ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,602,117	0	A	G	100.0%	475.1 / NA	127	I210V (ATT→GTT)	lsrA	autoinducer 2 import ATP‑binding protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (72/55); total (72/55)

Only 100 of 127 total aligned reads displayed.

ACGCTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGG  >  NC_000913/1602020‑1602212

aCGCTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGtt                                                                                               <  1:3007820/100‑1 (MQ=255)

aCGCTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGtt                                                                                               <  1:1963239/100‑1 (MQ=255)

 cGCTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTa                                                                                              <  2:1868026/100‑1 (MQ=255)

  gCTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAg                                                                                             <  2:1522343/100‑1 (MQ=255)

   cTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGc                                                                                            >  1:813023/1‑100 (MQ=255)

     tGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGt                                                                                          <  2:255039/100‑1 (MQ=255)

      gTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTg                                                                                         <  2:963551/100‑1 (MQ=255)

      gTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTg                                                                                         >  2:1563613/1‑100 (MQ=255)

      gTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTg                                                                                         <  2:692771/100‑1 (MQ=255)

           gTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGCGTTAgatcggaaga                                                                                    >  2:1549491/1‑91 (MQ=255)

           gTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATgcg                                                                                    >  1:608083/1‑100 (MQ=255)

           gTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATgcg                                                                                    >  2:1624716/1‑100 (MQ=255)

           gTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATgcg                                                                                    >  2:1557821/1‑100 (MQ=255)

           gTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATgcg                                                                                    >  1:2908956/1‑100 (MQ=255)

           gTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATgcg                                                                                    >  2:1010328/1‑100 (MQ=255)

            tCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATgcgc                                                                                   >  1:2007400/1‑100 (MQ=255)

             cGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGcgcg                                                                                  >  2:743634/1‑100 (MQ=255)

              gCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGa                                                                                 >  1:2552074/1‑100 (MQ=255)

               cTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGAc                                                                                <  1:2068333/100‑1 (MQ=255)

                 tGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACgg                                                                              >  2:499213/1‑100 (MQ=255)

                 tGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACgg                                                                              >  2:2478563/1‑100 (MQ=255)

                  gCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGa                                                                             <  2:1510067/100‑1 (MQ=255)

                   cAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGaa                                                                            >  2:1516194/1‑100 (MQ=255)

                     agagCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAAcc                                                                          >  1:2856839/1‑100 (MQ=255)

                       agCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCAt                                                                        <  1:1180916/100‑1 (MQ=255)

                        gctgctTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATc                                                                       >  2:875658/1‑100 (MQ=255)

                           gcttgctACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGcc                                                                    <  2:2856828/100‑1 (MQ=255)

                           gcttgctACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGcc                                                                    >  2:1904143/1‑100 (MQ=255)

                           gcttgctACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGcc                                                                    >  1:2097782/1‑100 (MQ=255)

                            cttgctACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCt                                                                   >  1:522502/1‑100 (MQ=255)

                            cttgctACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCt                                                                   >  2:707187/1‑100 (MQ=255)

                             ttgctACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCtt                                                                  >  2:2876003/1‑100 (MQ=255)

                                 tACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGc                                                              >  2:2715739/1‑100 (MQ=255)

                                 tACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGc                                                              >  1:754163/1‑100 (MQ=255)

                                  aCTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCg                                                             >  2:2690353/1‑100 (MQ=255)

                                   cTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCgg                                                            >  1:3095195/1‑100 (MQ=255)

                                    tGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGc                                                           <  2:2348405/100‑1 (MQ=255)

                                     ggCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCa                                                          >  2:694304/1‑100 (MQ=255)

                                     ggCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCa                                                          >  1:3374533/1‑100 (MQ=255)

                                     ccgatctgTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCa                                                          <  2:3067691/93‑1 (MQ=255)

                                       tgTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCaaa                                                        <  1:2483671/99‑1 (MQ=255)

                                       cGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCaaa                                                        >  1:2133804/1‑100 (MQ=255)

                                        gTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCaaaa                                                       >  2:2483663/1‑100 (MQ=255)

                                          gggTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAAcc                                                     >  1:139078/1‑100 (MQ=255)

                                          gggTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAAcc                                                     <  1:1894260/100‑1 (MQ=255)

                                           ggTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCa                                                    >  1:745107/1‑100 (MQ=255)

                                            gTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCaagatcgg                                                   >  1:3067703/1‑94 (MQ=255)

                                              aTTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGcn                                                 <  2:1222084/100‑2 (MQ=255)

                                              aTTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCg                                                 <  2:1126106/100‑1 (MQ=255)

                                                tGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGaa                                               <  1:2690362/100‑1 (MQ=255)

                                                 gTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGaga                                              >  1:973011/1‑98 (MQ=255)

                                                 gTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAAc                                              >  1:2262635/1‑100 (MQ=255)

                                                  tttttATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACt                                             >  1:324150/1‑100 (MQ=255)

                                                  tttttATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACt                                             >  2:2922469/1‑100 (MQ=255)

                                                  tttttATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACt                                             >  1:873910/1‑100 (MQ=255)

                                                  tttttATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACt                                             <  1:2876014/100‑1 (MQ=255)

                                                        tctcGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTAc                                       >  2:1984664/1‑100 (MQ=255)

                                                         ctcGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTAcc                                      <  2:1947621/100‑1 (MQ=255)

                                                         ctcGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTAcc                                      >  2:213974/1‑100 (MQ=255)

                                                         ctcGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTAcc                                      >  1:1837169/1‑100 (MQ=255)

                                                         ctcGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTAcc                                      <  1:2384293/100‑1 (MQ=255)

                                                         ctcGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTAcc                                      >  2:1331281/1‑100 (MQ=255)

                                                         ctcGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTAcc                                      <  1:2690893/100‑1 (MQ=255)

                                                         ctcGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTAc                                       <  1:1984668/99‑1 (MQ=255)

                                                          tcGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACcg                                     >  2:3295459/1‑100 (MQ=255)

                                                            gCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACcgac                                   <  2:1641561/100‑1 (MQ=255)

                                                             cATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACcgacg                                  <  1:3295472/100‑1 (MQ=255)

                                                              aTAAGCCGCGGGAAGCTCGCCAGCGTGCCGAGCGAGTTAGCGGGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACcgacga                                 <  2:3228086/100‑1 (MQ=255)

                                                               tAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCgacgac                                >  1:3368380/1‑100 (MQ=255)

                                                                aaGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCgacgac                                <  1:88812/99‑1 (MQ=255)

                                                                 aGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCGTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACat                              <  2:2097778/100‑1 (MQ=255)

                                                                 aGCTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACat                              >  2:1990295/1‑100 (MQ=255)

                                                                   cTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACatta                            <  2:3095183/100‑1 (MQ=255)

                                                                   cTGCCGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACatta                            >  1:2267467/1‑100 (MQ=255)

                                                                   cTGCCGGAAATTCGCCAGATTGCCGACCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACatta                            >  1:2070483/1‑100 (MQ=255)

                                                                      ccGGAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTc                         >  1:2982223/1‑100 (MQ=255)

                                                                         gAAATTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAgg                      <  1:1968346/100‑1 (MQ=255)

                                                                          gatctCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGc                     <  1:1119305/96‑1 (MQ=255)

                                                                           taTTCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGcc                    <  1:631154/99‑1 (MQ=255)

                                                                             ttCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCAt                  <  2:3374520/100‑1 (MQ=255)

                                                                             ttCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCAt                  <  1:707187/100‑1 (MQ=255)

                                                                              tCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATc                 <  1:1010329/100‑1 (MQ=255)

                                                                              tCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATc                 <  2:2017467/100‑1 (MQ=255)

                                                                              tCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATc                 >  2:625148/1‑100 (MQ=255)

                                                                              tCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATc                 <  2:522501/100‑1 (MQ=255)

                                                                              tCGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATc                 >  2:1256885/1‑100 (MQ=255)

                                                                               cGCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGcagatc                >  2:1119303/1‑95 (MQ=255)

                                                                                tgtgctcttccGATCTAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCAc               <  1:758428/91‑1 (MQ=255)

                                                                                tctaGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCAc               <  1:890219/97‑1 (MQ=255)

                                                                                gCCAGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCAc               <  2:139079/100‑1 (MQ=255)

                                                                                  cAGATTGCCGCTCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACCTTATTCAGGCCATCAccc             >  2:3255437/1‑100 (MQ=255)

                                                                                   aGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCAcccc            >  1:3161141/1‑100 (MQ=255)

                                                                                   aGATTGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCAcaga            >  2:890219/1‑97 (MQ=255)

                                                                                      ttGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGc         >  2:1656118/1‑100 (MQ=255)

                                                                                       tGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCg        <  2:2935186/100‑1 (MQ=255)

                                                                                       tGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCg        <  2:1291083/100‑1 (MQ=255)

                                                                                       tGCCGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCg        >  1:432244/1‑100 (MQ=255)

                                                                                         ccGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGt      <  1:2922480/100‑1 (MQ=255)

                                                                                          cGATCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTa     <  1:1656120/100‑1 (MQ=255)

                                                                                             tCGAGTTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACgg  <  1:1557824/100‑1 (MQ=255)

ACGCTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGG  >  NC_000913/1602020‑1602212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`