Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,615,188 A→G T129A (ACT→GCT)  yneJ → putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,615,1880AG98.4% 464.9 / ‑2.7 129T129A (ACT→GCT) yneJputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (54/72);  total (56/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 129 total aligned reads displayed.

GGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACGGGATACCGGTATACCGCG  >  NC_000913/1615091‑1615284
                                                                                                 |                                                                                                
ggAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCt                                                                                                <  1:2206328/100‑1 (MQ=255)
    aGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGc                                                                                            <  2:2939584/100‑1 (MQ=255)
     gCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGc                                                                                            <  2:3064518/99‑1 (MQ=255)
      cACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTg                                                                                          <  1:628509/100‑1 (MQ=255)
       aCCGCTGCAGTGCGCATTCCAGCCACTCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTgg                                                                                         <  2:2416830/100‑1 (MQ=255)
       aCCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTgg                                                                                         >  2:250689/1‑100 (MQ=255)
        ccGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGa                                                                                        >  2:708074/1‑100 (MQ=255)
          gCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATg                                                                                      <  1:1323092/100‑1 (MQ=255)
          gCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATg                                                                                      <  2:969952/100‑1 (MQ=255)
           cTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATgg                                                                                     <  2:753908/100‑1 (MQ=255)
             gCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGgtg                                                                                   >  1:1346321/1‑100 (MQ=255)
             gCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGgtg                                                                                   >  1:549223/1‑100 (MQ=255)
             gCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGgtg                                                                                   >  1:3345854/1‑100 (MQ=255)
              cAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGgtgt                                                                                  <  2:2647830/100‑1 (MQ=255)
                gTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTAc                                                                                >  1:356775/1‑100 (MQ=255)
                gTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTAc                                                                                <  1:667095/100‑1 (MQ=255)
                 tGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACt                                                                               <  1:2724735/100‑1 (MQ=255)
                  gcgcATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTg                                                                              <  2:2886058/100‑1 (MQ=255)
                  gcgcATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTg                                                                              <  2:2640747/100‑1 (MQ=255)
                  gcgcATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGGTGTACTg                                                                              >  2:2897113/1‑100 (MQ=255)
                   cgcATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTgg                                                                             <  1:3048520/100‑1 (MQ=255)
                   cgcATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTgg                                                                             >  2:2146301/1‑100 (MQ=255)
                       ttCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAggg                                                                         >  1:355560/1‑100 (MQ=255)
                         ccAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGaa                                                                       >  2:1465048/1‑100 (MQ=255)
                          cAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGaaa                                                                      >  2:386281/1‑100 (MQ=255)
                          cAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGaaa                                                                      <  2:1607116/100‑1 (MQ=255)
                           aGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGaaaa                                                                     >  2:1750830/1‑100 (MQ=255)
                            gCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAAc                                                                    >  1:1200957/1‑100 (MQ=255)
                            gCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGGTGTACTGGAGGGAAAAc                                                                    <  1:2486250/100‑1 (MQ=255)
                             ccACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGCCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACt                                                                   >  2:248968/1‑100 (MQ=255)
                              cACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTg                                                                  <  1:1634232/100‑1 (MQ=255)
                               aCGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGa                                                                 >  2:3013520/1‑100 (MQ=255)
                                cGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGaa                                                                <  2:243341/100‑1 (MQ=255)
                                 gCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAAt                                                               <  2:2158499/100‑1 (MQ=255)
                                  cTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATg                                                              <  2:1055538/100‑1 (MQ=255)
                                  cTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATg                                                              >  2:2041177/1‑100 (MQ=255)
                                    ggCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTTTGCTGGATGGTGTACTGGAGGGAAAACTGAATgcg                                                            >  2:464577/1‑100 (MQ=255)
                                    ggCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATgcg                                                            <  2:1908084/100‑1 (MQ=255)
                                    ggCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATgcg                                                            >  1:2795310/1‑100 (MQ=255)
                                        gAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGt                                                        <  2:2144292/100‑1 (MQ=255)
                                          aTTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGttt                                                      <  2:789795/100‑1 (MQ=255)
                                            tCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTAt                                                    <  2:3394576/100‑1 (MQ=255)
                                             cAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTAtt                                                   <  2:3239031/100‑1 (MQ=255)
                                             cAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTAtt                                                   >  1:1576474/1‑100 (MQ=255)
                                              aaCCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTg                                                  <  1:377473/100‑1 (MQ=255)
                                                ccGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGAt                                                <  1:3013532/100‑1 (MQ=255)
                                                ccGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGAt                                                <  1:1211153/100‑1 (MQ=255)
                                                 cgtcgtTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATg                                               <  1:708074/100‑1 (MQ=255)
                                                 cgtcgtTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATg                                               <  1:860109/100‑1 (MQ=255)
                                                 cgtcgtTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATg                                               >  1:942399/1‑100 (MQ=255)
                                                 cgtcgtTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATg                                               <  2:3241737/100‑1 (MQ=255)
                                                    cgtTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGAc                                            >  1:3169071/1‑100 (MQ=255)
                                                     gtTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGAcc                                           <  1:2708616/100‑1 (MQ=255)
                                                     gtTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGAcc                                           <  2:1637590/100‑1 (MQ=255)
                                                       tctCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCa                                         <  1:1959921/98‑1 (MQ=255)
                                                       tATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCa                                         <  2:3302057/100‑1 (MQ=255)
                                                       tATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCa                                         <  2:355559/100‑1 (MQ=255)
                                                         tCCCCAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCAtt                                       <  2:3135448/100‑1 (MQ=255)
                                                         tCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCAtt                                       >  2:1480602/1‑100 (MQ=255)
                                                         tCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCAtt                                       <  1:2161613/100‑1 (MQ=255)
                                                          cccAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGAGATATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGAcacaa                                        >  2:1959917/1‑97 (MQ=255)
                                                           ccAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTaa                                     >  2:2051704/1‑100 (MQ=255)
                                                           ccAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTaa                                     >  1:1256985/1‑100 (MQ=255)
                                                           ccAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTaa                                     >  1:2172018/1‑100 (MQ=255)
                                                           ccAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTaa                                     >  2:2703898/1‑100 (MQ=255)
                                                            cAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAAc                                    <  1:1725538/100‑1 (MQ=255)
                                                            cAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAAc                                    >  2:1631474/1‑100 (MQ=255)
                                                             aaaaTTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAAcc                                   <  1:2857758/100‑1 (MQ=255)
                                                             aaaaTTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAAcc                                   <  1:2413493/100‑1 (MQ=255)
                                                              aaaTTCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCa                                  <  1:1750834/100‑1 (MQ=255)
                                                                  tCAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACt                              <  2:3036003/100‑1 (MQ=255)
                                                                   cAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTg                             <  2:1053396/100‑1 (MQ=255)
                                                                   cAGTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTg                             <  1:1671923/100‑1 (MQ=255)
                                                                     gTTTTCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGcc                           >  2:3152607/1‑100 (MQ=255)
                                                                      ttttCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCa                          <  2:3381125/100‑1 (MQ=255)
                                                                        ttCACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATc                        <  1:290646/100‑1 (MQ=255)
                                                                          cACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGa                      >  1:425970/1‑100 (MQ=255)
                                                                          cACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGa                      >  1:736418/1‑100 (MQ=255)
                                                                          cACTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGa                      >  2:2335093/1‑100 (MQ=255)
                                                                           aCTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGAc                     >  2:3040989/1‑100 (MQ=255)
                                                                           aCTTTCCACCGGCCCTTCCGCCCCTATGCTGGATGGTGTACCGGAGGGAAAACTGAATGCGGCGATTATTGATGGACCCATTAACCATACTGCCATCGAc                     >  2:1483448/1‑100 (MQ=255)
                                                                            cTTTCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACg                    >  1:1857228/1‑100 (MQ=255)
                                                                             tttCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGTGATAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATCCTGCCATCGACgg                   <  1:1132169/100‑1 (MQ=255)
                                                                             tttCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACgg                   <  1:386282/100‑1 (MQ=255)
                                                                             tttCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACgg                   >  2:1318469/1‑100 (MQ=255)
                                                                              ttCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACggg                  <  1:250690/100‑1 (MQ=255)
                                                                              ttCCACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACggg                  <  2:356774/100‑1 (MQ=255)
                                                                                ccACCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGACATCGACGGGAt                >  2:1591516/1‑100 (MQ=255)
                                                                                  aCCGGCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACGGGATAc              >  1:3016557/1‑100 (MQ=255)
                                                                                     ggCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACGGGATACCgg           >  2:885562/1‑100 (MQ=255)
                                                                                     ggCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACGGGATACCgg           <  1:1539819/100‑1 (MQ=255)
                                                                                      gCCCTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACGGGATACCGGt          <  2:884401/100‑1 (MQ=255)
                                                                                       cccTTCCGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACGGGATACCGGta         >  2:1111083/1‑100 (MQ=255)
                                                                                             cGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACGGGATACCGGTATACcgc   >  2:2004252/1‑100 (MQ=255)
                                                                                             cGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACGGGATACCGGTATACcgc   <  1:1079930/100‑1 (MQ=255)
                                                                                             cGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACGGGATACCGGTATACcgc   <  1:426551/100‑1 (MQ=255)
                                                                                             cGGCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACGGGATACCGGTATACcgc   >  1:3266174/1‑100 (MQ=255)
                                                                                              ggCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACGGGATACCGGTATACcgcg  >  2:2085595/1‑100 (MQ=255)
                                                                                              ggCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACGGGATACCGGTATACcgcg  >  2:2923887/1‑100 (MQ=255)
                                                                                              ggCGCTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACGGGATACCGGTATACcgcg  >  2:2924096/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGGTGTACTGGAGGGAAAACTGAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATCGACGGGATACCGGTATACCGCG  >  NC_000913/1615091‑1615284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: