Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,127,857 G→A P153L (CCG→CTG)  gmd ← GDP‑D‑mannose dehydratase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,127,8570GA98.4% 424.7 / ‑2.5 122P153L (CCG→CTG) gmdGDP‑D‑mannose dehydratase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (64/56);  total (65/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

Only 100 of 130 total aligned reads displayed.

GAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGAC  >  NC_000913/2127760‑2127953
                                                                                                 |                                                                                                 
ttccgatcttCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCagg                                                                                                 <  2:2713957/92‑1 (MQ=255)
tccgatctttCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCagg                                                                                                 <  1:2034946/92‑1 (MQ=255)
gAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCagg                                                                                                 >  2:1492581/1‑100 (MQ=255)
gAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCagg                                                                                                 <  1:272423/100‑1 (MQ=255)
  agagCATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGt                                                                                                <  1:2205067/99‑1 (MQ=255)
  agagAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTa                                                                                               >  2:1864061/1‑100 (MQ=255)
  agagAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTa                                                                                               >  1:314584/1‑100 (MQ=255)
     gaATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGaa                                                                                            >  2:1942196/1‑100 (MQ=255)
      aaTTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAAc                                                                                           <  1:2571700/100‑1 (MQ=255)
       aTTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACg                                                                                          >  2:2871995/1‑100 (MQ=255)
       aTTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACg                                                                                          >  2:2717625/1‑100 (MQ=255)
        ttCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACgg                                                                                         >  1:1394528/1‑100 (MQ=255)
        ttCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGAACGCATACGGAGATCGCAGGTAGAACgg                                                                                         <  2:1519442/100‑1 (MQ=255)
         tCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACgg                                                                                         >  2:125181/1‑99 (MQ=255)
           cGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGt                                                                                      <  1:1208574/100‑1 (MQ=255)
                  ggCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTctct                                                                               >  2:413213/1‑100 (MQ=255)
                  ggCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTctct                                                                               >  2:1579487/1‑100 (MQ=255)
                     atACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTc                                                                            <  2:2701907/99‑1 (MQ=255)
                        cATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGc                                                                         >  1:543606/1‑100 (MQ=255)
                         aTGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCg                                                                       >  1:2103940/1‑100 (MQ=255)
                         aTGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCg                                                                       <  1:1273742/100‑1 (MQ=255)
                         aTGCCGTAGGATGCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGGGGTCTCTTTCTGCg                                                                       >  2:2716965/1‑100 (MQ=255)
                          tGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCgg                                                                      <  1:2891597/100‑1 (MQ=255)
                          tGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCgg                                                                      >  1:2487336/1‑100 (MQ=255)
                            ccGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGaa                                                                    <  1:1864065/100‑1 (MQ=255)
                             cGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAAt                                                                   >  1:3066722/1‑100 (MQ=255)
                              gTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAAtt                                                                  >  2:2015145/1‑100 (MQ=255)
                               tAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAAttt                                                                 >  1:973096/1‑100 (MQ=255)
                                aGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTc                                                                >  2:471548/1‑100 (MQ=255)
                                aGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTc                                                                <  1:3356316/100‑1 (MQ=255)
                                aGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTc                                                                >  2:3286076/1‑100 (MQ=255)
                                 ggATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGTGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAAttttc                                                               <  1:2300029/100‑3 (MQ=255)
                                  gATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCt                                                              <  1:3006797/100‑1 (MQ=255)
                                  gATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCt                                                              <  2:125059/100‑1 (MQ=255)
                                   aTTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTg                                                             <  2:126203/100‑1 (MQ=255)
                                    ttCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGc                                                            >  2:2153078/1‑100 (MQ=255)
                                    ttCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGc                                                            >  2:2152598/1‑100 (MQ=255)
                                     tCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCa                                                           <  2:1881682/100‑1 (MQ=255)
                                      cACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCAc                                                          >  1:1921172/1‑100 (MQ=255)
                                         ggTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAg                                                       >  2:1824200/1‑100 (MQ=255)
                                          gTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGa                                                      <  1:1579490/100‑1 (MQ=255)
                                           tAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGAc                                                     <  2:3327921/100‑1 (MQ=255)
                                            aGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCAGGAAATTccgcagaaagag                                                     <  1:2362181/100‑11 (MQ=255)
                                            aGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGAcc                                                    <  2:741722/100‑1 (MQ=255)
                                            aGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGAcc                                                    <  2:314583/100‑1 (MQ=255)
                                            aGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGAc                                                     >  1:578193/1‑99 (MQ=255)
                                             gTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCa                                                   >  2:1678534/1‑100 (MQ=255)
                                             gTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCa                                                   <  2:2581304/100‑1 (MQ=255)
                                             gTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCa                                                   >  1:2552603/1‑100 (MQ=255)
                                               tAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa                                                 <  2:3117762/100‑1 (MQ=255)
                                                  cGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAg                                              >  1:1021574/1‑100 (MQ=255)
                                                   ggTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGt                                             >  1:1421916/1‑100 (MQ=255)
                                                     atctCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTc                                           <  2:1961869/97‑1 (MQ=255)
                                                      gATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATAAAGTTCa                                          >  1:1749993/1‑100 (MQ=255)
                                                       aTCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCag                                         >  2:205885/1‑100 (MQ=255)
                                                       aTCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCag                                         >  2:727156/1‑100 (MQ=255)
                                                        tCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCaga                                        <  1:3391589/100‑1 (MQ=255)
                                                        cttccgatctgTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCaga                                        <  2:1032239/90‑1 (MQ=255)
                                                         ccAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCagat                                       >  1:1961873/1‑99 (MQ=255)
                                                             tAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTgg                                   <  1:3286089/100‑1 (MQ=255)
                                                                  gTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCagaagatcggaa                               >  1:1032240/1‑90 (MQ=255)
                                                                  gTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGcc                              <  1:711542/100‑1 (MQ=255)
                                                                  gTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGcc                              <  1:135124/100‑1 (MQ=255)
                                                                  gTACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGcc                              >  1:2510342/1‑100 (MQ=255)
                                                                   tACAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCt                             >  1:1770260/1‑100 (MQ=255)
                                                                    aCAGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTg                            <  1:1636825/100‑1 (MQ=255)
                                                                      aGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGAt                          <  1:275271/100‑1 (MQ=255)
                                                                      aGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGAt                          <  2:46340/100‑1 (MQ=255)
                                                                      aGTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGAt                          <  1:1040417/100‑1 (MQ=255)
                                                                       gTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATa                         <  1:1495524/100‑1 (MQ=255)
                                                                       gTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATa                         >  1:2969483/1‑100 (MQ=255)
                                                                       gTTTGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATa                         <  1:907570/100‑1 (MQ=255)
                                                                        tttGGCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAg                        <  1:624480/100‑1 (MQ=255)
                                                                           ggCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGaaa                     >  2:3014158/1‑100 (MQ=255)
                                                                            gCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAAc                    <  2:543605/100‑1 (MQ=255)
                                                                            gCGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAAc                    >  2:3047791/1‑100 (MQ=255)
                                                                             cGACCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACg                   >  2:1333088/1‑100 (MQ=255)
                                                                               aCCGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAg                 <  2:1394525/100‑1 (MQ=255)
                                                                                ccGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGATCAGAGGTGGAAGCCTGATAGAAACGAg                 >  2:743596/1‑99 (MQ=255)
                                                                                 cGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGtt               >  2:254299/1‑100 (MQ=255)
                                                                                 cGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGtt               >  2:2281199/1‑100 (MQ=255)
                                                                                 cGCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGtt               >  1:1388378/1‑100 (MQ=255)
                                                                                  gCATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGttt              >  1:457208/1‑100 (MQ=255)
                                                                                   cATACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGtttt             >  1:2186058/1‑100 (MQ=255)
                                                                                    tctcGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGttttc            <  2:310542/97‑1 (MQ=255)
                                                                                    aTACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGttttc            <  2:3338490/100‑1 (MQ=255)
                                                                                    aTACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGttttc            <  1:3014170/100‑1 (MQ=255)
                                                                                     tACGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGttttct           >  1:2954164/1‑100 (MQ=255)
                                                                                      aCGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGttttctt          <  2:2487328/100‑1 (MQ=255)
                                                                                       cGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGttttcttt         <  2:1311051/100‑1 (MQ=255)
                                                                                       cGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGttttcttt         >  1:1584207/1‑100 (MQ=255)
                                                                                       cGGAGATCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGttttcaga         >  1:310543/1‑97 (MQ=255)
                                                                                          agaTCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTcc      <  1:2891361/100‑1 (MQ=255)
                                                                                          agaTCGCAGGTAGAACGGCGTGGTCTCTTTCTGCCGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTcc      <  2:1801209/100‑1 (MQ=255)
                                                                                           gaTCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCa     >  1:1306095/1‑100 (MQ=255)
                                                                                            aTCGCAGGTAGAACGGTGTGGTCTCTGTCTTCGGAATTTCTTTCACCAGACCATACTGTTCAGAGGTGTAAGCCTGATAGAAACGCGTTTCCTTTTCCAg    <  2:143181/100‑1 (MQ=255)
                                                                                             tCGCAGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGa   >  2:1744050/1‑100 (MQ=255)
                                                                                              cgcaGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGAc  >  1:599653/1‑100 (MQ=255)
                                                                                              cgcaGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGAc  >  1:907162/1‑100 (MQ=255)
                                                                                              cgcaGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGAc  >  1:2094962/1‑100 (MQ=255)
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GAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGAC  >  NC_000913/2127760‑2127953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: