BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	410,479	A→G	S112S (TCA→TCG)	mak →	manno(fructo)kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	410,479	0	A	G	99.1%	394.3 / ‑4.8	108	S112S (TCA→TCG)	mak	manno(fructo)kinase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (49/58); total (50/58)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 108 total aligned reads displayed.

CAACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCG  >  NC_000913/410382‑410575

cAACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGa                                                                                                >  1:2782375/1‑100 (MQ=255)

cAACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGa                                                                                                <  1:1424181/100‑1 (MQ=255)

  cgatctcTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAg                                                                                              <  1:1017110/94‑1 (MQ=255)

  aCCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAg                                                                                              <  1:1082403/100‑1 (MQ=255)

  aCCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAg                                                                                              <  2:2906393/100‑1 (MQ=255)

   cttccgatctcGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGc                                                                                             <  1:1674595/90‑1 (MQ=255)

   ccTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCGAATGACGCTAACTGTCTGGCGGTTTCGGAAGc                                                                                             <  2:324976/100‑1 (MQ=255)

   ccTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGc                                                                                             <  1:1991451/100‑1 (MQ=255)

   ccTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGc                                                                                             <  1:305369/100‑1 (MQ=255)

     tGGCTCAACGGTCAGCCATTCGATAAAGACTTAGGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGCCGCTAACTGTCTGGCGGTTTCGGAAGCAg                                                                                           >  2:2049928/1‑100 (MQ=255)

     tGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAg                                                                                           <  1:1736897/100‑1 (MQ=255)

      ggCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGt                                                                                          >  2:1362652/1‑100 (MQ=255)

       gCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGTCGCTAACTGTCTGGCGGTTTCGGAAGCAGTa                                                                                         <  1:2292064/100‑1 (MQ=255)

       gCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTa                                                                                         <  1:2779355/100‑1 (MQ=255)

        cTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAgagatcg                                                                                        >  2:1017109/1‑94 (MQ=255)

        cTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAg                                                                                        >  1:1942263/1‑100 (MQ=255)

         tCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAg                                                                                        <  1:2627666/99‑1 (MQ=255)

         tCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGa                                                                                       <  2:1871323/100‑1 (MQ=255)

          cAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGAt                                                                                      <  2:1003516/100‑1 (MQ=255)

           aaCGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATg                                                                                     <  2:2115008/100‑1 (MQ=255)

           aaCGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATg                                                                                     <  2:2961817/100‑1 (MQ=255)

            aCGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATgg                                                                                    <  2:1390930/100‑1 (MQ=255)

              ggTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGgcg                                                                                  >  2:192388/1‑100 (MQ=255)

               gTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGCCGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGgcgc                                                                                 >  1:261733/1‑100 (MQ=255)

               gTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGgcgc                                                                                 >  2:232593/1‑100 (MQ=255)

                 atctcATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCgg                                                                               <  1:2775985/96‑1 (MQ=255)

                  ttccgatctaTAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGc                                                                              <  1:3209953/91‑1 (MQ=255)

                  aGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTCGATGGCGCGGc                                                                              >  2:89536/1‑100 (MQ=255)

                   gCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCa                                                                             >  1:593444/1‑100 (MQ=255)

                   gCCATTCGATAAAGACTTAAGCGCGAGGTTGCAACGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCa                                                                             >  1:208224/1‑100 (MQ=255)

                     cATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGgcgctcccgc                                                                           >  2:474521/1‑94 (MQ=255)

                     cATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCggagat                                                                           >  2:2775974/1‑96 (MQ=255)

                      aTTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCg                                                                          >  2:1239251/1‑100 (MQ=255)

                      aTTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCg                                                                          >  2:133435/1‑100 (MQ=255)

                      aTTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCg                                                                          <  1:1959630/100‑1 (MQ=255)

                       ttCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCgg                                                                         <  1:2905661/100‑1 (MQ=255)

                        ctcttccgatcTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCggg                                                                        <  2:1976496/90‑1 (MQ=255)

                         cGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGa                                                                       >  1:2566771/1‑100 (MQ=255)

                          gATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAg                                                                      <  1:1239254/100‑1 (MQ=255)

                           aTAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAgatcggaa                                                                     >  2:3209940/1‑93 (MQ=255)

                           aTAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAgc                                                                     <  2:2122740/100‑1 (MQ=255)

                              aaGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCa                                                                  >  2:2570577/1‑100 (MQ=255)

                              aaGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCa                                                                  >  2:1906615/1‑100 (MQ=255)

                              aaGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGAAGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGGgcgc                                                                   >  2:2380603/1‑99 (MQ=255)

                                gACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGa                                                                >  2:3097570/1‑100 (MQ=255)

                                 aCTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGAc                                                               <  1:3376359/100‑1 (MQ=255)

                                 aCTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGAc                                                               >  2:557788/1‑100 (MQ=255)

                                 aCTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGAc                                                               <  1:1241125/100‑1 (MQ=255)

                                  cTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACg                                                              >  2:1157155/1‑100 (MQ=255)

                                    tAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAgatcggaagag                                                            >  1:1976500/1‑90 (MQ=255)

                                    tAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGt                                                            >  1:2112805/1‑100 (MQ=255)

                                    tAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGt                                                            >  1:2112309/1‑100 (MQ=255)

                                      aGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTAt                                                          >  2:142633/1‑100 (MQ=255)

                                       gcgcGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTAtt                                                         <  2:2529686/100‑1 (MQ=255)

                                       gcgcGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTAtn                                                         <  2:423426/100‑2 (MQ=255)

                                             ggTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGt                                                   <  2:1110436/100‑1 (MQ=255)

                                             ggTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGt                                                   <  1:2838662/100‑1 (MQ=255)

                                                tGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGAt                                                <  2:959387/100‑1 (MQ=255)

                                                tGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGAt                                                <  1:232594/100‑1 (MQ=255)

                                                 gCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGAtt                                               >  1:735598/1‑100 (MQ=255)

                                                  cAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTa                                              >  2:195090/1‑100 (MQ=255)

                                                    gCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATc                                            >  2:423351/1‑100 (MQ=255)

                                                     cGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCg                                           <  1:2380610/100‑1 (MQ=255)

                                                      gggAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCgg                                          <  1:142632/100‑1 (MQ=255)

                                                           gTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACgg                                     >  1:373780/1‑100 (MQ=255)

                                                                  tGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATgcgg                              <  1:2948106/100‑1 (MQ=255)

                                                                  tGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATgcgg                              <  1:2570585/100‑1 (MQ=255)

                                                                    gCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATgcggcg                            >  2:2903608/1‑100 (MQ=255)

                                                                     cAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGgcgc                           >  2:193093/1‑100 (MQ=255)

                                                                       aaTGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCgg                         <  2:593443/100‑1 (MQ=255)

                                                                           aCGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGt                     <  1:45394/100‑1 (MQ=255)

                                                                             gCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTgg                   <  1:3097582/100‑1 (MQ=255)

                                                                              ttAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGc                  <  2:1200156/99‑1 (MQ=255)

                                                                              cTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGc                  >  2:3204929/1‑100 (MQ=255)

                                                                               tAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGCCGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGAggta                 >  2:1446074/1‑98 (MQ=255)

                                                                               tAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCa                 <  2:261732/100‑1 (MQ=255)

                                                                               tAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCa                 <  1:1994399/100‑1 (MQ=255)

                                                                               tAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCa                 >  1:1200159/1‑100 (MQ=255)

                                                                                aaCTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCAt                <  1:2033714/100‑1 (MQ=255)

                                                                                 aCTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCAtt               <  1:3204942/100‑1 (MQ=255)

                                                                                 aCTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCAtt               <  1:405401/100‑1 (MQ=255)

                                                                                 aCTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCAtt               >  2:801454/1‑100 (MQ=255)

                                                                                  cTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTc              <  1:1245526/100‑1 (MQ=255)

                                                                                    gTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCaa            >  1:762990/1‑100 (MQ=255)

                                                                                     tcgggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAAt           <  2:2026765/97‑1 (MQ=255)

                                                                                      cTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATg          >  2:2070746/1‑100 (MQ=255)

                                                                                      cTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGCGGCATTCAATg          <  1:1157158/100‑1 (MQ=255)

                                                                                       tGGCGTTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATg          <  2:252319/99‑1 (MQ=255)

                                                                                        ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCagatcggaag        >  1:986155/1‑91 (MQ=255)

                                                                                        ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATggg        >  2:2664295/1‑100 (MQ=255)

                                                                                        ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATggg        >  1:1505273/1‑100 (MQ=255)

                                                                                        ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATggg        <  2:3390141/100‑1 (MQ=255)

                                                                                        ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATggg        <  1:742189/100‑1 (MQ=255)

                                                                                        ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATggg        >  2:1156419/1‑100 (MQ=255)

                                                                                          cGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcg      <  2:182743/100‑1 (MQ=255)

                                                                                           ggTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcgg     >  1:1161331/1‑100 (MQ=255)

                                                                                            gTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTGTCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcggg    >  2:2400087/1‑100 (MQ=255)

                                                                                            gTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcggg    <  1:195091/100‑1 (MQ=255)

                                                                                             tttCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcgggc   <  1:2370569/100‑1 (MQ=255)

                                                                                              ttCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGggcgggcg  >  1:3308430/1‑100 (MQ=255)

CAACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCG  >  NC_000913/410382‑410575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`