Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 420,470 A→G T294A (ACG→GCG)  brnQ → branched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913420,4700AG98.8% 590.7 / ‑2.7 161T294A (ACG→GCG) brnQbranched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (78/81);  total (79/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 161 total aligned reads displayed.

AACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAACGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTCGGCGGCTTCTCGAT  >  NC_000913/420374‑420567
                                                                                                |                                                                                                 
aaCGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCgg                                                                                                >  2:1257435/1‑100 (MQ=255)
aaCGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCgg                                                                                                <  1:327636/100‑1 (MQ=255)
 aCGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGc                                                                                               <  1:3318219/100‑1 (MQ=255)
 aCGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGc                                                                                               <  1:2970270/100‑1 (MQ=255)
  cGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCgg                                                                                                >  2:2940466/1‑98 (MQ=255)
  cGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCa                                                                                              >  2:905/1‑100 (MQ=255)
    gcgcTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGt                                                                                            <  2:2222461/100‑1 (MQ=255)
    gcgcTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGt                                                                                            <  1:2679430/100‑1 (MQ=255)
     cgcTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGtt                                                                                           <  2:2456706/100‑1 (MQ=255)
     cgcTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGtt                                                                                           <  1:302147/100‑1 (MQ=255)
      gctgctATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTg                                                                                          >  1:2581714/1‑100 (MQ=255)
       ctgctATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTgg                                                                                         >  1:1297502/1‑100 (MQ=255)
       ctgctATTCGGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTg                                                                                          <  2:675044/99‑1 (MQ=255)
        tgctATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGc                                                                                        >  2:2223309/1‑100 (MQ=255)
        tgctATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGc                                                                                        >  1:977216/1‑100 (MQ=255)
         gctATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGcc                                                                                       <  2:1432053/100‑1 (MQ=255)
         gctATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGcc                                                                                       >  1:939915/1‑100 (MQ=255)
         gctATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGcc                                                                                       <  1:3369131/100‑1 (MQ=255)
          ctATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCt                                                                                      >  1:2797587/1‑100 (MQ=255)
           tATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTg                                                                                     >  1:3249176/1‑100 (MQ=255)
            aTTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGa                                                                                    >  2:3038322/1‑100 (MQ=255)
              tCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGAcc                                                                                  <  1:3150293/100‑1 (MQ=255)
                tGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCtg                                                                                >  2:1864133/1‑100 (MQ=255)
                tGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCtg                                                                                <  2:2929619/100‑1 (MQ=255)
                tGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCtg                                                                                >  1:2040425/1‑100 (MQ=255)
                tGCATGCTTAAGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCAGCGTCTGCCTGGTAGCGGCAGTTGGCCTGACCtg                                                                                <  2:2241131/100‑1 (MQ=255)
                  cATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCtgtg                                                                              >  2:45067/1‑100 (MQ=255)
                    tGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCt                                                                            >  1:2573150/1‑100 (MQ=255)
                    tGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCt                                                                            <  2:1964347/100‑1 (MQ=255)
                      cTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTtg                                                                          <  1:1012439/100‑1 (MQ=255)
                      cTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTtg                                                                          <  2:201351/100‑1 (MQ=255)
                       ttACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTtgt                                                                         >  1:1723377/1‑100 (MQ=255)
                        tACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTtgtg                                                                        >  2:2168232/1‑100 (MQ=255)
                         aCGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGc                                                                       <  2:2484303/100‑1 (MQ=255)
                         aCGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGc                                                                       <  2:3029357/100‑1 (MQ=255)
                          cGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCa                                                                      >  1:80932/1‑100 (MQ=255)
                          cGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCa                                                                      <  1:1251361/100‑1 (MQ=255)
                           gTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAg                                                                     <  2:2107077/100‑1 (MQ=255)
                           gTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAg                                                                     >  2:354066/1‑100 (MQ=255)
                             tCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGaa                                                                   <  1:882520/100‑1 (MQ=255)
                              cAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAAt                                                                  >  2:182044/1‑100 (MQ=255)
                              cAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAAt                                                                  >  2:461474/1‑100 (MQ=255)
                              cAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAAt                                                                  <  2:3134892/100‑1 (MQ=255)
                               aGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAAtt                                                                 >  2:2988208/1‑100 (MQ=255)
                                   cacCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCttt                                                             >  2:2598898/1‑100 (MQ=255)
                                   cacCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCttt                                                             >  1:2187944/1‑100 (MQ=255)
                                   cacCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCttt                                                             >  1:2926485/1‑100 (MQ=255)
                                   cacCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCttt                                                             <  2:1538784/100‑1 (MQ=255)
                                   cacCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCttt                                                             <  2:326342/100‑1 (MQ=255)
                                    acCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTg                                                            <  1:72338/100‑1 (MQ=255)
                                    acCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTg                                                            <  2:3319326/100‑1 (MQ=255)
                                     ccTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGc                                                           >  1:2267744/1‑100 (MQ=255)
                                     ccTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGc                                                           <  1:1150055/100‑1 (MQ=255)
                                     ccTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGc                                                           <  1:1927398/100‑1 (MQ=255)
                                     ccTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGc                                                           <  1:3116798/100‑1 (MQ=255)
                                     ccTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGc                                                           <  1:482152/100‑1 (MQ=255)
                                      cTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTgtc                                                          <  2:1751181/100‑3 (MQ=255)
                                      cTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGcc                                                          <  1:3287624/100‑1 (MQ=255)
                                       tttGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGccc                                                         <  2:3331055/100‑1 (MQ=255)
                                           gcggcggcgGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAgta                                                     >  2:3112709/1‑100 (MQ=255)
                                           gcggcggcgGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAgta                                                     <  2:3238933/100‑1 (MQ=255)
                                           gcggcggcgGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAgta                                                     <  1:1363811/100‑1 (MQ=255)
                                            cggcggcggTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACTTGTGCTTGTGCAGAATTCTTTGCCCAgtac                                                    <  2:2670336/100‑1 (MQ=255)
                                            cggcggcggTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAgtac                                                    <  1:2223315/100‑1 (MQ=255)
                                             ggcggcggTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAgtacg                                                   >  1:1836/1‑100 (MQ=255)
                                                 gcggTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTAcc                                               <  1:1048788/100‑1 (MQ=255)
                                                 gcggTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTAcc                                               <  1:3395169/100‑1 (MQ=255)
                                                    gTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGct                                            >  1:1220198/1‑100 (MQ=255)
                                                        cTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGctctct                                        <  2:2267738/100‑1 (MQ=255)
                                                        cTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGctctct                                        >  1:1974671/1‑100 (MQ=255)
                                                        cTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGctctct                                        <  2:1974667/100‑1 (MQ=255)
                                                        cTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGctctct                                        <  1:905/100‑1 (MQ=255)
                                                        cTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGctctct                                        <  1:1870609/100‑1 (MQ=255)
                                                            ctgctgGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATc                                    >  1:2767397/1‑100 (MQ=255)
                                                             tgctgGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCg                                   >  1:314939/1‑100 (MQ=255)
                                                              gctgGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGCTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGt                                  <  1:1298647/100‑1 (MQ=255)
                                                                tgGCGGCGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTAc                                <  2:728476/100‑1 (MQ=255)
                                                                 ggcggcGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACg                               >  2:2330073/1‑100 (MQ=255)
                                                                 ggcggcGTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACg                               >  2:507097/1‑100 (MQ=255)
                                                                  gcggcgTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTAc                                <  2:2068844/98‑1 (MQ=255)
                                                                   cggcgTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCt                             >  2:2648423/1‑100 (MQ=255)
                                                                   atctgTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCt                             <  2:1625498/96‑1 (MQ=255)
                                                                       gTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCtagat                         >  1:1625500/1‑96 (MQ=255)
                                                                       gTTAATCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGgtg                         <  1:1343346/100‑1 (MQ=255)
                                                                          aaTCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGttt                      >  1:742192/1‑100 (MQ=255)
                                                                            tCTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTAt                    <  2:372154/100‑1 (MQ=255)
                                                                             cTTCATCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATc                   >  2:368801/1‑100 (MQ=255)
                                                                                 aTCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTcg               >  1:712972/1‑100 (MQ=255)
                                                                                  tCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTcgg              >  2:2800340/1‑100 (MQ=255)
                                                                                  tCGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTcgg              >  2:242717/1‑100 (MQ=255)
                                                                                   cGCCTGCCTGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTcggc             <  2:2581706/100‑1 (MQ=255)
                                                                                    gcctgcctGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTcggcg            >  2:138881/1‑100 (MQ=255)
                                                                                    gcctgcctGGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTcggcg            >  2:13511/1‑100 (MQ=255)
                                                                                       tgcctgGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTCGGCGGCt         <  2:1329474/100‑1 (MQ=255)
                                                                                       tgcctgGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTCGGCGGCt         <  1:3112721/100‑1 (MQ=255)
                                                                                       tgcctgGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAAGTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTCGGCGGCt         <  2:1158697/100‑1 (MQ=255)
                                                                                         cctgGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTCGGCGGCTtc       <  2:3249163/100‑1 (MQ=255)
                                                                                         cctgGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTCGGCGGCTtc       >  2:1115089/1‑100 (MQ=255)
                                                                                          ctgGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTCGGCGGCTtct      >  2:1061344/1‑100 (MQ=255)
                                                                                          ctgGTAGCGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTCGGCGGCTtct      >  1:2091970/1‑100 (MQ=255)
                                                                                                |                                                                                                 
AACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAACGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTCGGCGGCTTCTCGAT  >  NC_000913/420374‑420567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: