Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 426,471 G→A R112H (CGT→CAT)  tgt → tRNA‑guanine transglycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913426,4710GA98.3% 410.7 / ‑2.8 116R112H (CGT→CAT) tgttRNA‑guanine transglycosylase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (60/54);  total (61/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 116 total aligned reads displayed.

ATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTTGATGA  >  NC_000913/426374‑426567
                                                                                                 |                                                                                                
aTGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATa                                                                                                <  1:570942/100‑1 (MQ=255)
    aGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAAccc                                                                                            <  2:3049233/100‑1 (MQ=255)
    aGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAAccc                                                                                            <  1:366977/100‑1 (MQ=255)
     gTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCg                                                                                           >  1:2761520/1‑100 (MQ=255)
     gTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCg                                                                                           >  2:636371/1‑100 (MQ=255)
     gTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCg                                                                                           >  2:1957472/1‑100 (MQ=255)
        ctaGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATc                                                                                        <  1:173881/98‑1 (MQ=255)
         aaGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCa                                                                                       <  2:1802012/100‑1 (MQ=255)
          aGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCag                                                                                      >  2:173881/1‑99 (MQ=255)
          aGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCaa                                                                                      >  1:2105435/1‑100 (MQ=255)
           ggggCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAAc                                                                                     >  2:1215914/1‑100 (MQ=255)
             ggCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCAGCGAACAGGGCGTGCACTTCCATAACCCGATCAACgg                                                                                   >  1:3238334/1‑100 (MQ=255)
              gCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGc                                                                                  >  1:3146018/1‑100 (MQ=255)
                 gATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGAt                                                                               >  1:2902581/1‑100 (MQ=255)
                   tCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATcc                                                                             <  1:2331815/100‑1 (MQ=255)
                   tCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATcc                                                                             >  2:2486316/1‑100 (MQ=255)
                    ccTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATcc                                                                             <  2:1019307/99‑1 (MQ=255)
                     cTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGa                                                                           >  2:2975948/1‑100 (MQ=255)
                     cTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGa                                                                           >  1:1950764/1‑100 (MQ=255)
                     cTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGa                                                                           >  2:1075351/1‑100 (MQ=255)
                      tCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGAt                                                                          <  2:2111323/100‑1 (MQ=255)
                      tCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGAt                                                                          >  2:718912/1‑100 (MQ=255)
                       cACTGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGAtt                                                                         >  1:830192/1‑100 (MQ=255)
                       cACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGAtt                                                                         >  2:1565397/1‑100 (MQ=255)
                       cACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATACTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCTACGGCGATCCGAtt                                                                         >  2:2312782/1‑100 (MQ=255)
                        aCCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACACGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGAttt                                                                        >  1:1424704/1‑100 (MQ=255)
                               ccGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGa                                                                 >  1:106371/1‑100 (MQ=255)
                               ccGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGa                                                                 >  2:314864/1‑100 (MQ=255)
                                cggcggCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGAt                                                                <  1:1957476/100‑1 (MQ=255)
                                  cttccgatctggTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATcc                                                              <  1:742481/90‑1 (MQ=255)
                                   cggcTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCt                                                             <  2:3238321/100‑1 (MQ=255)
                                    ggcTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTg                                                            >  1:38708/1‑100 (MQ=255)
                                       ttCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGaaa                                                         <  1:89962/100‑1 (MQ=255)
                                         ccAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGaaaaa                                                       <  2:2807740/100‑1 (MQ=255)
                                         ccAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGaaaaa                                                       <  2:3359615/100‑1 (MQ=255)
                                         ccAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGaaaaa                                                       >  2:2058612/1‑100 (MQ=255)
                                         ccAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGaaaaa                                                       >  1:574460/1‑100 (MQ=255)
                                           aGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACTGCGATACGGTTTTCCTCGATCCTGAAAAAt                                                      >  2:3189565/1‑99 (MQ=255)
                                           aGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATc                                                     <  1:351146/100‑1 (MQ=255)
                                           aGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATc                                                     <  2:2985418/100‑1 (MQ=255)
                                           aGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATc                                                     >  2:1597987/1‑100 (MQ=255)
                                            ggTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATccagatcggaag                                                    >  2:742481/1‑90 (MQ=255)
                                            ggTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCa                                                    >  2:3336202/1‑100 (MQ=255)
                                              tCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGCTTTTCCTCGATCCTGAAAAATCAAt                                                  >  1:1310219/1‑100 (MQ=255)
                                              tCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAAt                                                  <  2:1965737/100‑1 (MQ=255)
                                              tCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAAt                                                  <  1:2771144/100‑1 (MQ=255)
                                               cTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATg                                                 >  1:2088470/1‑100 (MQ=255)
                                                ttCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATgg                                                <  1:326498/100‑1 (MQ=255)
                                                ttCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATgg                                                <  1:2440676/100‑1 (MQ=255)
                                                ttCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATgg                                                >  2:1022568/1‑100 (MQ=255)
                                                 tCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGga                                               >  2:1023680/1‑100 (MQ=255)
                                                    gCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGAt                                            >  1:2989108/1‑100 (MQ=255)
                                                     ccTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGAACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGAtt                                           >  2:1821263/1‑100 (MQ=255)
                                                      cttccgatctttCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTc                                          <  2:1329143/90‑1 (MQ=255)
                                                       ttGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCa                                         <  2:795434/100‑1 (MQ=255)
                                                        tGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAg                                        <  2:2902570/100‑1 (MQ=255)
                                                           cGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTAc                                     >  1:2758928/1‑100 (MQ=255)
                                                            gATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACg                                    >  1:880893/1‑100 (MQ=255)
                                                                ttCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAgatcggaag                                >  1:1329146/1‑92 (MQ=255)
                                                                ttCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCt                                >  2:2480048/1‑100 (MQ=255)
                                                                 tCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCtt                               >  1:887106/1‑100 (MQ=255)
                                                                 tCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCtt                               >  1:2660897/1‑100 (MQ=255)
                                                                  cGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTg                              >  2:277317/1‑100 (MQ=255)
                                                                  cGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTg                              >  1:571730/1‑100 (MQ=255)
                                                                  cGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCCAGCCGATTTTACTCGATCATGAAAAATCAATGGAGATAAAGTACTATTTTg                              >  1:1476808/1‑100 (MQ=255)
                                                                   gTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTgg                             <  2:1888215/100‑1 (MQ=255)
                                                                   gTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTgg                             <  2:38708/100‑1 (MQ=255)
                                                                   gTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTgg                             <  2:3273206/100‑1 (MQ=255)
                                                                   gTAAAATCACCAAACAGGGCGTGCACTTCCATAACCCGATCAACGGCTATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTgg                             <  1:2370848/100‑1 (MQ=255)
                                                                    tAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGt                            <  2:1409936/100‑1 (MQ=255)
                                                                    tAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGt                            <  1:1128155/100‑1 (MQ=255)
                                                                      aaaTCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTc                          >  2:2925752/1‑100 (MQ=255)
                                                                        aTCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCgg                        <  2:562669/100‑1 (MQ=255)
                                                                        aTCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCgg                        <  2:1766084/100‑1 (MQ=255)
                                                                         tCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGa                       <  1:2312788/100‑1 (MQ=255)
                                                                           aCCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGata                     <  1:2972512/100‑1 (MQ=255)
                                                                           aCCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGata                     >  2:2460781/1‑100 (MQ=255)
                                                                            ccGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTTGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGatat                    >  2:3383487/1‑100 (MQ=255)
                                                                             cGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATc                   >  2:1466959/1‑100 (MQ=255)
                                                                              gAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCg                  >  1:1463853/1‑100 (MQ=255)
                                                                              gAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCg                  <  1:3184162/100‑1 (MQ=255)
                                                                              gAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCg                  <  2:2105431/100‑1 (MQ=255)
                                                                               aaCAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGt                 <  1:1850671/100‑1 (MQ=255)
                                                                                aCAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTc                >  2:29428/1‑100 (MQ=255)
                                                                                 cAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCa               <  2:2758917/100‑1 (MQ=255)
                                                                                      cgatctcTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATc          <  2:254362/94‑1 (MQ=255)
                                                                                      cGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATc          <  2:1424701/100‑1 (MQ=255)
                                                                                       gttcACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCt         <  2:587913/97‑1 (MQ=255)
                                                                                       gTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCagatcgg         >  1:1655312/1‑94 (MQ=255)
                                                                                       gTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCt         >  1:894370/1‑100 (MQ=255)
                                                                                         gCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCttt       <  2:3029822/100‑1 (MQ=255)
                                                                                         gCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCttt       <  2:1310216/100‑1 (MQ=255)
                                                                                          cACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTtg      <  1:314865/100‑1 (MQ=255)
                                                                                           aCTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTtg      <  2:2936188/99‑1 (MQ=255)
                                                                                            cTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATcagatcg    >  1:254363/1‑94 (MQ=255)
                                                                                            cTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTtgat    >  2:3331975/1‑100 (MQ=255)
                                                                                            cTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTtgat    >  2:348736/1‑100 (MQ=255)
                                                                                            cTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTtgat    >  2:1529386/1‑100 (MQ=255)
                                                                                             ttCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTtgatg   <  1:1215917/100‑1 (MQ=255)
                                                                                              tCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTtgatga  >  2:1857521/1‑100 (MQ=255)
                                                                                                 |                                                                                                
ATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTTGATGA  >  NC_000913/426374‑426567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑