| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 220,255 | Δ1 bp | 100% | coding (674/816 nt) | metQ ← | DL‑methionine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 220,252 | 0 | G | . | 52.9% | 100.6 / 150.6 | 68 | coding (677/816 nt) | metQ | DL‑methionine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (14/18); new base . (16/20); total (30/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.46e-01 | |||||||||||
GCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCG > NC_000913/220153‑220348 | gctgctTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTtacgtacg > 2:14180/1‑100 (MQ=255) ctgctTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACgg < 1:1925521/100‑1 (MQ=255) tgctTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACggg > 2:196587/1‑100 (MQ=255) tgctTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACggg < 2:1635574/100‑1 (MQ=255) gctTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACgggg < 2:599611/100‑1 (MQ=255) cGTAAACTTCGTCAGACTGATAAGCCTTGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACtggactc > 1:370531/1‑95 (MQ=255) cGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGActct < 2:1911/100‑1 (MQ=255) gTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGActct > 2:166447/1‑100 (MQ=255) aaaCTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCttt > 2:1213180/1‑100 (MQ=255) aaaCTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTa < 2:970421/100‑1 (MQ=255) aaaCTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTa < 1:1495919/100‑1 (MQ=255) aCTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATc < 2:2393044/100‑1 (MQ=255) cTTCGTCAGACTGTTAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATc > 2:1760133/1‑100 (MQ=255) cTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATc < 2:399883/100‑1 (MQ=255) cTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCt > 2:877754/1‑100 (MQ=255) cGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTc > 2:358265/1‑100 (MQ=255) cGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTca > 1:1222081/1‑100 (MQ=255) cGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTca < 2:2266406/100‑1 (MQ=255) gTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGTCTCTTTATCttga < 2:1756964/100‑3 (MQ=255) gTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTcaa > 1:2033739/1‑100 (MQ=255) cttccgatcttAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTca < 2:1053740/90‑1 (MQ=255) tCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTcaa < 2:1555111/100‑1 (MQ=255) cAGACTGATAAGCCTGTACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTcaaca < 1:669764/100‑1 (MQ=255) acctaTAAGCCTGGACGAATTTCTTCTCGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTGCGTAC‑GGGACTCTTTATCTTcaaca < 1:1498295/93‑1 (MQ=255) aTAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATAc > 2:1457237/1‑100 (MQ=255) tAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTcaagatcggaag > 1:1053737/1‑91 (MQ=255) aGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCg > 2:823100/1‑100 (MQ=255) gCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGt < 2:400319/100‑1 (MQ=255) ccTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCt > 1:2287930/1‑100 (MQ=255) ccTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCt > 1:963526/1‑100 (MQ=255) cTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCtt > 1:478930/1‑100 (MQ=255) gACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCg < 2:2397790/100‑1 (MQ=255) cGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGc < 2:1158641/100‑1 (MQ=255) cGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGcc > 1:1842349/1‑100 (MQ=255) cGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGcc > 1:1821969/1‑100 (MQ=255) cGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGcc > 1:1220471/1‑100 (MQ=255) aaTTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGAACAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCgg > 1:1434738/1‑100 (MQ=255) tttCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGCCTTTCGCCGGAg < 2:1699155/100‑1 (MQ=255) ttcttcACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAg > 2:1233663/1‑100 (MQ=255) cttcACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAAGTTTACGTACGGCGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTc < 1:1196189/100‑1 (MQ=255) ttcACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAg > 1:1053969/1‑100 (MQ=255) cACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAgg < 2:635853/100‑1 (MQ=255) aCGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGc > 1:1208001/1‑100 (MQ=255) aCGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGcc < 1:2017914/100‑1 (MQ=255) cGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGcc < 2:1001774/100‑1 (MQ=255) gTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGcc < 1:783633/98‑1 (MQ=255) gTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCaa > 2:1925267/1‑100 (MQ=255) ctcGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATc < 1:2249193/100‑1 (MQ=255) ctcGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATct < 2:1809143/100‑1 (MQ=255) tcGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATct > 1:1779966/1‑100 (MQ=255) cGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctg < 2:1895766/100‑1 (MQ=255) ggCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggc < 2:700447/100‑1 (MQ=255) cGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctggctn < 2:529098/100‑2 (MQ=255) tCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCtggctggc > 2:566590/1‑100 (MQ=255) cTTTGTTATTTTCAATCGTCACGATCATATTTACGTAC‑GAGACTCTTTATTTTCAACAAAGTTACCGTCTTTCGCCGCAGTCAGGCCAATctggctggta < 1:1357996/100‑3 (MQ=255) ttGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCAt > 2:1594228/1‑100 (MQ=255) gTTATCTTCACGCGTGACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAg < 2:2555574/100‑1 (MQ=255) ttATCTTCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAggt > 1:297442/1‑100 (MQ=255) tATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAggt > 1:2449739/1‑100 (MQ=255) tATCTTCACGCGTCACGATCAGGTTTACGTACGGGGAATCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAagt > 1:109522/1‑100 (MQ=255) cTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAggtggt > 2:1259303/1‑100 (MQ=255) ttCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTAt < 1:1287417/100‑1 (MQ=255) tCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTAt < 1:1045030/100‑1 (MQ=255) tCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTAt < 1:1732355/100‑1 (MQ=255) tCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTAtt > 2:54277/1‑100 (MQ=255) tCACGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTAtt < 2:1281469/100‑1 (MQ=255) aCGCGTCACGATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGa < 2:852734/100‑1 (MQ=255) cgTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATa < 2:1276205/100‑1 (MQ=255) tCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAAc < 1:1259299/100‑1 (MQ=255) cACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACt > 1:1134365/1‑100 (MQ=255) gATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGcc < 2:398230/100‑1 (MQ=255) gATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGcc < 1:897719/100‑1 (MQ=255) gATCAGGTTTACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCTGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCa < 1:694901/100‑1 (MQ=255) tttACGTAC‑GGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGAt < 1:1925257/100‑1 (MQ=255) tacgtac‑gGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGAttt > 2:1637089/1‑100 (MQ=255) cgtac‑gGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTgc > 2:1717442/1‑100 (MQ=255) gtacgGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTgc > 1:415111/1‑100 (MQ=255) gtacgGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCACAGGCGGTATTGATAACTGCCAGAGCGCTTTgc > 1:56795/1‑100 (MQ=255) tacgGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTgcg > 2:771751/1‑100 (MQ=255) | GCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCG > NC_000913/220153‑220348 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |